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5595896 Expression of heterologous genes in transgenic plants and plant cells using plant asparagine synthetase promoters
792
PATENT ABSTRACTS
micelle-forming agent, and an oil. This antigen formulation is preferably lacking in an immunostimulating peptide component, or has sufficiently low levels of such a component that the desired CTL response is not diminished. This formulation is provided as a stable oil-in-water emulsion.
5585544 M E T H O D OF CAUSING SOMATIC HYBRIDIZATION B E T W E E N T W O SPECIES OF ALGAE Cheney Donald P; Wang Le Zhong Ipswich, MA, UNITED STATES Assigned to Northeastern University A method of causing somatic cell hybridization between two species of algae by growing somatic algal tissue from each of the two species of algae in close proximity, in a nutrient solution, and isolating and culturing the hybrid somatic shoots that form.
5591616 METHOD FOR TRANSFORMING MONOCOTYLEDONS Hiei Yokoh; Komari Toshihiko lwata gun, JAPAN Assigned to Japan Tobacco Inc PCT No. PCT/JP93/00925 Sec. 371 Date May 3, 1994 Sec. 102(e) Date May 3, 1994 PCT Filed Jul. 6, 1993 PCT Pub. No. WO94/00977 PCT Pub. Date Jan. 20, 1994. A method for transforming a monocotyledon by which the time required from transformation to regeneration of a plant is shorter so that the frequency of emergence of mutants is smaller than the conventional methods, which may be generally applied even to the plants for which the regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledon comprising transforming a cultured tissue during de,differentiation process or a dedifferentiated cultured tissue of said monocotyledon with a bacterium belonging to
genus Agrobacterium containing a desired gene.
5595896 EXPRESSION OF H E T E R O L O G O U S GENES IN TRANSGENIC PLANTS A N D PLANT CELLS USING PLANT ASPARAGINE S Y N T H E T A S E PROMOTERS Comzzi Gloria; Tsai Fong-Ying New York, NY, UNITED STATES Assigned to New York University The identification and cloning of the gene(s) for plant asparagine synthetase Assigned to, an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.
5596131 R E G E N E R A T I O N OF G R A M I N A C E O U S PLANTS OF THE SUBFAMILY P O O I D E A E F R O M PROTOPLASTS Horn Michael E; Harms Christian T; Shillito Raymond Woodland, CA, UNITED STATES Assigned to Ciba-Geigy Corporation The present invention provides cell cultures derived from graminaceous plants which cultures are capable of being regenerated into plants, including fertile plants. Methods of accomplishing this regeneration are also provided. In addition, a novel procedure for the cryopreservation of embryogenic cell cultures is described.
PATENT ABSTRACTS
micelle-forming agent, and an oil. This antigen formulation is preferably lacking in an immunostimulating peptide component, or has sufficiently low levels of such a component that the desired CTL response is not diminished. This formulation is provided as a stable oil-in-water emulsion.
5585544 M E T H O D OF CAUSING SOMATIC HYBRIDIZATION B E T W E E N T W O SPECIES OF ALGAE Cheney Donald P; Wang Le Zhong Ipswich, MA, UNITED STATES Assigned to Northeastern University A method of causing somatic cell hybridization between two species of algae by growing somatic algal tissue from each of the two species of algae in close proximity, in a nutrient solution, and isolating and culturing the hybrid somatic shoots that form.
5591616 METHOD FOR TRANSFORMING MONOCOTYLEDONS Hiei Yokoh; Komari Toshihiko lwata gun, JAPAN Assigned to Japan Tobacco Inc PCT No. PCT/JP93/00925 Sec. 371 Date May 3, 1994 Sec. 102(e) Date May 3, 1994 PCT Filed Jul. 6, 1993 PCT Pub. No. WO94/00977 PCT Pub. Date Jan. 20, 1994. A method for transforming a monocotyledon by which the time required from transformation to regeneration of a plant is shorter so that the frequency of emergence of mutants is smaller than the conventional methods, which may be generally applied even to the plants for which the regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledon comprising transforming a cultured tissue during de,differentiation process or a dedifferentiated cultured tissue of said monocotyledon with a bacterium belonging to
genus Agrobacterium containing a desired gene.
5595896 EXPRESSION OF H E T E R O L O G O U S GENES IN TRANSGENIC PLANTS A N D PLANT CELLS USING PLANT ASPARAGINE S Y N T H E T A S E PROMOTERS Comzzi Gloria; Tsai Fong-Ying New York, NY, UNITED STATES Assigned to New York University The identification and cloning of the gene(s) for plant asparagine synthetase Assigned to, an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.
5596131 R E G E N E R A T I O N OF G R A M I N A C E O U S PLANTS OF THE SUBFAMILY P O O I D E A E F R O M PROTOPLASTS Horn Michael E; Harms Christian T; Shillito Raymond Woodland, CA, UNITED STATES Assigned to Ciba-Geigy Corporation The present invention provides cell cultures derived from graminaceous plants which cultures are capable of being regenerated into plants, including fertile plants. Methods of accomplishing this regeneration are also provided. In addition, a novel procedure for the cryopreservation of embryogenic cell cultures is described.