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Antiinflammatory and immunomodulatory effects of I-modulia, an Aquaphilus dolomiae extract, on atopic dermatitis in vitro
P8372
P8568
A 3-week moisturization study followed by a 2-week regression phase to evaluate the efficacy of a triple oat skin protectant cream versus a ceramide cream on moderate to severe dry skin Judith Nebus, MBA, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Michael Suero, Johnson & Johnson Malaysai, Petaling Jaya, Selangor, Malaysia; Warren Wallo, MS, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States Background: Flaking, roughness, and pruritus are common problems among patients with extra dry skin. Daily application of moisturizing creams with ingredients, such as skin protectants, humectants, and emollients is important to enhance skin water content and protect the skin barrier. Colloidal oatmeal is a natural skin protectant that contains a variety of components, including avenanthramides, that bind to the skin to provide a protective barrier. The purpose of this 5week study was to compare the effectiveness of a skin protectant moisturizing cream containing colloidal oatmeal, oat oil, and avenanthramides (triple oat cream) with a leading ceramide moisturizing cream in improving moisture content and barrier function in individuals with moderate to severe xerosis on the lower legs.
Antiinflammatory and immunomodulatory effects of I-modulia, an Aquaphilus dolomiae extract, on atopic dermatitis in vitro Marie-Franc¸oise Aries, PhD, Pierre Fabre DermoCosmetique, Toulouse, France; Clemence Vaissiere, Pierre Fabre DermoCosmetique, Toulouse, France; Helene Delga, Pierre Fabre DermoCosmetique, Toulouse, France; Marguerite Lev^eque, Pierre Fabre DermoCosmetique, Toulouse, France; Nathalie Castex-Rizzi, PhD, Pierre Fabre DermoCosmetique, Toulouse, France; Sandrine Bessou-Touya, PhD, Pierre Fabre DermoCosmetique, Toulouse, France; Thien Nguyen, PhD, Pierre Fabre DermoCosmetique, Toulouse, France
Methods: Subjects with moderate to severe xerosis on the lower legs used either the triple oat cream or ceramide cream twice a day for 21 days (treatment phase). On days 22 to 34 (regression phase) subjects did not use any treatment moisturizer on their legs. Transepidermal water loss (TEWL) and moisture content/hydration (Corneometer) were measured at baseline and various times during the study. Results: Thirty-five subjects completed the study. Significant improvements from baseline in TEWL measurements were observed (P \ .05) at all time points (treatment and regression phases) in groups using either product. However, the group using triple oat cream had greater improvements in TEWL (P\.05) than those using the ceramide cream on days 7 and 14. Use of either product, increased leg skin moisture content significantly from baseline (P \ .05) throughout both the treatment and regression phases. Consistent with the TEWL results, users of the triple oat cream had significantly higher leg skin moisture content compared with users of the ceramide cream at all time points during the treatment phase. Clinical evaluations for roughness showed a significant improvement from baseline at all time points (treatment and regression) for both treatment groups. Conclusions: Both the triple oat cream and ceramide cream improved skin barrier function (TEWL) and moisture content compared with baseline in individuals with moderate to severe xerosis on the lower legs. However, the triple oat cream provided signifantly greater benefits during this clinical trial than did the ceramide cream.
Atopic dermatitis (AD) is a chronic inflammatory skin disease with a complex pathophysiology, including genetic, immunologic and environmental factors interactions, and characterized by allergic inflammation, barrier disruption, and pruritic lesions. Keratinocytes which are active immunologic cells with major control over AD inflammation by means of cytokine/chemokine production contribute to the disease phenotype development. Likewise, TH1, TH2, TH17 cells which are commonly observed in AD lesions are postulated in the disease process. Emollient application makes the use of topical potent immune modulatory agents logical therapeutic consideration. The aim of the present study was to evaluate Imodulia, an original biologic extract from culture of Aquaphilus dolomiae, isolated from Avene Spring Thermal Water microflora, on different immune inflammatory cell models. Firstly, inflammatory mediators gene expression was assessed in Normal Human Keratinocytes (NHK) stimulated by the association polyI:C+IL4+IL13+TNFa and performed by Q-PCR 32 genes array. Secondly PAR-2 (protease-activated receptor-2) activity was assessed in NHK stimulated by trypsin and performed by Ca2+ flux signals monitoring. Finally TH1, TH2, TH17 cytokines basal production was assessed on CD4+ lymphocytes by 12-plex Luminex technology. Our results showed that I-modulia inhibited gene expression of numerous mediators including TSLP, IL18, IFNb1, RANTES, MCP3, TARC, MIP-3a, MDC, IL4R, and induced involucrin, psoriasin, RNase7 gene expression; I-modulia also inhibited the PAR-2 activation and the TH1, TH2, TH17 cytokines production (IL2, IFNg/IL4, IL5, IL13/IL17, respectively). Together, the present data support the high regulator activity of I-modulia, an A dolomiae extract, on keratinocyte inflammatory and lymphocyte immune responses, and reveal its powerful interest in topical preparations designed for the regulation of AD immune inflammatory pathology. Commercial support: None identified.
Sponsored 100% by Johnson & Johnson Consumer Companies, Inc.
P7926 Atopic dermatitis in adults: Association of impaired of CD38 and CD69 expression and eosinophilia. Tiago de Oliveira Titz, MMSc, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Camila de Lollo, MMSc, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Maria Notomi Sato, PhD, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Raquel Leao Orfali, MD, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Valeria Aoki, MD, PhD, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Vanessa Gonc¸alves dos Santos, MMSc, University of S~ao Paulo Medical School, S~ao Paulo, Brazil
P8062
Objective: The aim of this study was to evaluate the expression of activation markers on eosinophils from peripheral blood of atopic dermatitis (AD) patients and correlate them with disease severity.
Antiinflammatory activity of colloidal oat (Avena sativa) MIchael Southall, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Karien Rodriguez, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Khalid Mahmood, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Kurt Reynertson, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Michelle Garay, MS, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Simarna Kaur, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States Oats (Avena sativa) are one of the earliest domesticated crops, valued as food and medicine for centuries. Oatmeal baths, plasters, and extracts have a long history of use as a skin soothers and topical antiinflammatory agents. Colloidal oatmeal—finely ground whole oat kernels—is a monographed ingredient in the US FDA Skin Protectant monograph. Oats contains a high oil content, and are a rich source of unsaturated omega-3 linoleic and omega-6 linolenic acid triglycerides, mixed tocopherols and tocotrienols, beta-glucan, phenolics, starches, and proteins. Despite centuries of use many of the biochemical mechanisms that make colloidal oats beneficial to the skin remain unclear. Therefore, this study investigated the mechanisms of colloidal oat activity on human keratinocytes. Extracts of colloidal oat were found to significantly attenuate the UV-induced production of reactive oxygen species from primary human keratinocytes. Furthermore, our studies determined that extracts of colloidal oat reduced the levels of the proinflammatory mediator, IL-8, in a UV-induced inflammation model of keratinocytes. Taken together, these results demonstrate that colloidal oats have direct antioxidant and antiinflammatory activity which may provide the therapeutic effect for dermatologic formulations containing colloidal oats.
Methods: This study enrolled patients with AD from Hospital das Clınicas, University of S~ao Paulo Medical School, diagnosed according to Hanifin and Rajka criteria. Out of the 12 patients included in the study, 2 were males and 10 females, aged between 21 and 61. Twenty healthy controls, aged between 24 and 53 years, 8 males and 13 females were also enrolled. Severity of AD was established according to EASI (Eczema Area and Severity Index, patients graded as mild (58.3%), moderate (16.6%) and severe (25%). Eosinophils (lineage cocktail 1- CCR3+) from peripheral blood were analyzed for CD38, CD69, CD23, and CD62L expression by flow cytometry (LSR Fortessa, BD Biosciences) and analysis was performed using Flow Jo 7.5.6 software. Results: Our findings revealed an increased frequency of CCR3+ eosinophils, and upregulation of CCR3 medium fluorescence intensity (MFI) in AD individuals, when compared to controls. As for the CD69 and CD38 expression, we detected an increased frequency in eosinophils, with diminished medium fluorescence intensity (MFI) in AD individuals, when compared to controls. No significant difference in CD23 and CD62L expression on eosinophils were detected in the analyzed groups. The findings were not associated with AD severity.
Sponsored 100% by Johnson & Johnson Consumer Companies, Inc.
Commercial support: None identified.
MAY 2014
Background: Atopic dermatitis is a chronic, recurrent inflammatory disease, with prevalence around 10% to 20% in children and 1% to 3% in adults. Elevated serum levels of eosinophils are frequently observed in AD patients, and may be implicated in the pathogenesis of this skin condition.
Conclusion: Eosinophilia is a hallmark of atopic dermatitis; however, augmented number of eosinophils may not reflect a normal functional profile of these inflammatory cells, once it is associated with decreased activation of CD38 and CD69 eosinophil markers.
J AM ACAD DERMATOL
AB61
P8568
A 3-week moisturization study followed by a 2-week regression phase to evaluate the efficacy of a triple oat skin protectant cream versus a ceramide cream on moderate to severe dry skin Judith Nebus, MBA, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Michael Suero, Johnson & Johnson Malaysai, Petaling Jaya, Selangor, Malaysia; Warren Wallo, MS, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States Background: Flaking, roughness, and pruritus are common problems among patients with extra dry skin. Daily application of moisturizing creams with ingredients, such as skin protectants, humectants, and emollients is important to enhance skin water content and protect the skin barrier. Colloidal oatmeal is a natural skin protectant that contains a variety of components, including avenanthramides, that bind to the skin to provide a protective barrier. The purpose of this 5week study was to compare the effectiveness of a skin protectant moisturizing cream containing colloidal oatmeal, oat oil, and avenanthramides (triple oat cream) with a leading ceramide moisturizing cream in improving moisture content and barrier function in individuals with moderate to severe xerosis on the lower legs.
Antiinflammatory and immunomodulatory effects of I-modulia, an Aquaphilus dolomiae extract, on atopic dermatitis in vitro Marie-Franc¸oise Aries, PhD, Pierre Fabre DermoCosmetique, Toulouse, France; Clemence Vaissiere, Pierre Fabre DermoCosmetique, Toulouse, France; Helene Delga, Pierre Fabre DermoCosmetique, Toulouse, France; Marguerite Lev^eque, Pierre Fabre DermoCosmetique, Toulouse, France; Nathalie Castex-Rizzi, PhD, Pierre Fabre DermoCosmetique, Toulouse, France; Sandrine Bessou-Touya, PhD, Pierre Fabre DermoCosmetique, Toulouse, France; Thien Nguyen, PhD, Pierre Fabre DermoCosmetique, Toulouse, France
Methods: Subjects with moderate to severe xerosis on the lower legs used either the triple oat cream or ceramide cream twice a day for 21 days (treatment phase). On days 22 to 34 (regression phase) subjects did not use any treatment moisturizer on their legs. Transepidermal water loss (TEWL) and moisture content/hydration (Corneometer) were measured at baseline and various times during the study. Results: Thirty-five subjects completed the study. Significant improvements from baseline in TEWL measurements were observed (P \ .05) at all time points (treatment and regression phases) in groups using either product. However, the group using triple oat cream had greater improvements in TEWL (P\.05) than those using the ceramide cream on days 7 and 14. Use of either product, increased leg skin moisture content significantly from baseline (P \ .05) throughout both the treatment and regression phases. Consistent with the TEWL results, users of the triple oat cream had significantly higher leg skin moisture content compared with users of the ceramide cream at all time points during the treatment phase. Clinical evaluations for roughness showed a significant improvement from baseline at all time points (treatment and regression) for both treatment groups. Conclusions: Both the triple oat cream and ceramide cream improved skin barrier function (TEWL) and moisture content compared with baseline in individuals with moderate to severe xerosis on the lower legs. However, the triple oat cream provided signifantly greater benefits during this clinical trial than did the ceramide cream.
Atopic dermatitis (AD) is a chronic inflammatory skin disease with a complex pathophysiology, including genetic, immunologic and environmental factors interactions, and characterized by allergic inflammation, barrier disruption, and pruritic lesions. Keratinocytes which are active immunologic cells with major control over AD inflammation by means of cytokine/chemokine production contribute to the disease phenotype development. Likewise, TH1, TH2, TH17 cells which are commonly observed in AD lesions are postulated in the disease process. Emollient application makes the use of topical potent immune modulatory agents logical therapeutic consideration. The aim of the present study was to evaluate Imodulia, an original biologic extract from culture of Aquaphilus dolomiae, isolated from Avene Spring Thermal Water microflora, on different immune inflammatory cell models. Firstly, inflammatory mediators gene expression was assessed in Normal Human Keratinocytes (NHK) stimulated by the association polyI:C+IL4+IL13+TNFa and performed by Q-PCR 32 genes array. Secondly PAR-2 (protease-activated receptor-2) activity was assessed in NHK stimulated by trypsin and performed by Ca2+ flux signals monitoring. Finally TH1, TH2, TH17 cytokines basal production was assessed on CD4+ lymphocytes by 12-plex Luminex technology. Our results showed that I-modulia inhibited gene expression of numerous mediators including TSLP, IL18, IFNb1, RANTES, MCP3, TARC, MIP-3a, MDC, IL4R, and induced involucrin, psoriasin, RNase7 gene expression; I-modulia also inhibited the PAR-2 activation and the TH1, TH2, TH17 cytokines production (IL2, IFNg/IL4, IL5, IL13/IL17, respectively). Together, the present data support the high regulator activity of I-modulia, an A dolomiae extract, on keratinocyte inflammatory and lymphocyte immune responses, and reveal its powerful interest in topical preparations designed for the regulation of AD immune inflammatory pathology. Commercial support: None identified.
Sponsored 100% by Johnson & Johnson Consumer Companies, Inc.
P7926 Atopic dermatitis in adults: Association of impaired of CD38 and CD69 expression and eosinophilia. Tiago de Oliveira Titz, MMSc, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Camila de Lollo, MMSc, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Maria Notomi Sato, PhD, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Raquel Leao Orfali, MD, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Valeria Aoki, MD, PhD, University of S~ao Paulo Medical School, S~ao Paulo, Brazil; Vanessa Gonc¸alves dos Santos, MMSc, University of S~ao Paulo Medical School, S~ao Paulo, Brazil
P8062
Objective: The aim of this study was to evaluate the expression of activation markers on eosinophils from peripheral blood of atopic dermatitis (AD) patients and correlate them with disease severity.
Antiinflammatory activity of colloidal oat (Avena sativa) MIchael Southall, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Karien Rodriguez, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Khalid Mahmood, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Kurt Reynertson, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Michelle Garay, MS, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States; Simarna Kaur, PhD, Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, United States Oats (Avena sativa) are one of the earliest domesticated crops, valued as food and medicine for centuries. Oatmeal baths, plasters, and extracts have a long history of use as a skin soothers and topical antiinflammatory agents. Colloidal oatmeal—finely ground whole oat kernels—is a monographed ingredient in the US FDA Skin Protectant monograph. Oats contains a high oil content, and are a rich source of unsaturated omega-3 linoleic and omega-6 linolenic acid triglycerides, mixed tocopherols and tocotrienols, beta-glucan, phenolics, starches, and proteins. Despite centuries of use many of the biochemical mechanisms that make colloidal oats beneficial to the skin remain unclear. Therefore, this study investigated the mechanisms of colloidal oat activity on human keratinocytes. Extracts of colloidal oat were found to significantly attenuate the UV-induced production of reactive oxygen species from primary human keratinocytes. Furthermore, our studies determined that extracts of colloidal oat reduced the levels of the proinflammatory mediator, IL-8, in a UV-induced inflammation model of keratinocytes. Taken together, these results demonstrate that colloidal oats have direct antioxidant and antiinflammatory activity which may provide the therapeutic effect for dermatologic formulations containing colloidal oats.
Methods: This study enrolled patients with AD from Hospital das Clınicas, University of S~ao Paulo Medical School, diagnosed according to Hanifin and Rajka criteria. Out of the 12 patients included in the study, 2 were males and 10 females, aged between 21 and 61. Twenty healthy controls, aged between 24 and 53 years, 8 males and 13 females were also enrolled. Severity of AD was established according to EASI (Eczema Area and Severity Index, patients graded as mild (58.3%), moderate (16.6%) and severe (25%). Eosinophils (lineage cocktail 1- CCR3+) from peripheral blood were analyzed for CD38, CD69, CD23, and CD62L expression by flow cytometry (LSR Fortessa, BD Biosciences) and analysis was performed using Flow Jo 7.5.6 software. Results: Our findings revealed an increased frequency of CCR3+ eosinophils, and upregulation of CCR3 medium fluorescence intensity (MFI) in AD individuals, when compared to controls. As for the CD69 and CD38 expression, we detected an increased frequency in eosinophils, with diminished medium fluorescence intensity (MFI) in AD individuals, when compared to controls. No significant difference in CD23 and CD62L expression on eosinophils were detected in the analyzed groups. The findings were not associated with AD severity.
Sponsored 100% by Johnson & Johnson Consumer Companies, Inc.
Commercial support: None identified.
MAY 2014
Background: Atopic dermatitis is a chronic, recurrent inflammatory disease, with prevalence around 10% to 20% in children and 1% to 3% in adults. Elevated serum levels of eosinophils are frequently observed in AD patients, and may be implicated in the pathogenesis of this skin condition.
Conclusion: Eosinophilia is a hallmark of atopic dermatitis; however, augmented number of eosinophils may not reflect a normal functional profile of these inflammatory cells, once it is associated with decreased activation of CD38 and CD69 eosinophil markers.
J AM ACAD DERMATOL
AB61