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Antimutagenic action of some plant factors
236 chemically. It could be recovered in the acidic water-soluble fraction of the mushroom extract, was relatively thermostabile and non volatile.
136 Zdzienicka, M., M. Hryniewicz, M. Pieflkowska and T. Szymczyk, Warsaw Medical Academy, Warsaw (Poland) Antimutagenic action of some plant factors
The antimutagenic activity of various kinds of plant extracts has been investigated. Mutagenicity assay was performed with strains TA1538, TA98, TA100 of Salmonella typhimurium by the Ames standard method. Extracts from wheat, maize and pea sprouts inhibited mutagenic activity of benzo[a]pyrene and 2-acetylaminofluorene but had no effect on mutagenic activity of direct mutagens (MMS, MNNG). The results indicate that plant factors can modify metabolic activation of pro-mutagens into their active forms. The antimutagenic activity of the pea extract was thermolabile whereas those of wheat and maize extracts were heat resistant. Experiments on characterization of the above antimutagenic factors are in progress. Extracts from some common vegetables (carrot, parsley, lettuce) showed no antimutagenic activity in the system tested.
137 Zora, Z.S., S.A. Svetlana and R.B. Branislav, Boris Kidri~ Institute of Nuclear Sciences, Vin~a, Belgrade (Yugoslavia) The influence of Cerenkov light on spore-forming bacteria
It is assumed that one basic type of radiation-induced molecular lesion can be responsible for a variety of different biological effects. Recently, it was concluded that a large part of the photoreactivation that can be achieved after ionizing radiation involves repair of damage induced by UV Cerenkov light produced in the suspending medium. Photoreactivation of damage induced by T-rays depends on the type and energy of the ionizing radiation. However, relatively little is known about the biological consequences of Cerenkov light on spore-forming bacteria. Therefore experiments were performed to determine whether ionizing radiation-induced damage on Bacillus pumilus E601 could be repaired by photoreactivation by investigating the colony-forming ability after exposure to T- and Cerenkov radiations. The colonyforming ability of the irradiated cells completely recovered after 6 h holding in TGY broth and after 24 h holding in saline.
136 Zdzienicka, M., M. Hryniewicz, M. Pieflkowska and T. Szymczyk, Warsaw Medical Academy, Warsaw (Poland) Antimutagenic action of some plant factors
The antimutagenic activity of various kinds of plant extracts has been investigated. Mutagenicity assay was performed with strains TA1538, TA98, TA100 of Salmonella typhimurium by the Ames standard method. Extracts from wheat, maize and pea sprouts inhibited mutagenic activity of benzo[a]pyrene and 2-acetylaminofluorene but had no effect on mutagenic activity of direct mutagens (MMS, MNNG). The results indicate that plant factors can modify metabolic activation of pro-mutagens into their active forms. The antimutagenic activity of the pea extract was thermolabile whereas those of wheat and maize extracts were heat resistant. Experiments on characterization of the above antimutagenic factors are in progress. Extracts from some common vegetables (carrot, parsley, lettuce) showed no antimutagenic activity in the system tested.
137 Zora, Z.S., S.A. Svetlana and R.B. Branislav, Boris Kidri~ Institute of Nuclear Sciences, Vin~a, Belgrade (Yugoslavia) The influence of Cerenkov light on spore-forming bacteria
It is assumed that one basic type of radiation-induced molecular lesion can be responsible for a variety of different biological effects. Recently, it was concluded that a large part of the photoreactivation that can be achieved after ionizing radiation involves repair of damage induced by UV Cerenkov light produced in the suspending medium. Photoreactivation of damage induced by T-rays depends on the type and energy of the ionizing radiation. However, relatively little is known about the biological consequences of Cerenkov light on spore-forming bacteria. Therefore experiments were performed to determine whether ionizing radiation-induced damage on Bacillus pumilus E601 could be repaired by photoreactivation by investigating the colony-forming ability after exposure to T- and Cerenkov radiations. The colonyforming ability of the irradiated cells completely recovered after 6 h holding in TGY broth and after 24 h holding in saline.