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Indoleamine 2,3-Dioxygenase-Expressing Aortic Plasmacytoid Dendritic Cells Protect against Atherosclerosis by Induction of Regulatory T Cells
Cell Metabolism
Corrections Indoleamine 2,3-Dioxygenase-Expressing Aortic Plasmacytoid Dendritic Cells Protect against Atherosclerosis by Induction of Regulatory T Cells Tae Jin Yun, Jun Seong Lee, Kawthar Machmach, Dahee Shim, Junhee Choi, Young Jin Wi, Hyung Seok Jang, In-Hyuk Jung, Kyeongdae Kim, Won Kee Yoon, Mohammad Alam Miah, Bin Li, Jinsam Chang, Mariana G. Bego, Tram N.Q. Pham, Jakob Loschko, Jo¨rg Hermann Fritz, Anne B. Krug, Seung-Pyo Lee, Tibor Keler, Jean V. Guimond, Elie Haddad, Eric A. Cohen, Martin G. Sirois, Ismail El-Hamamsy, Marco Colonna, Goo Taeg Oh,* Jae-Hoon Choi,* and Cheolho Cheong* *Correspondence: [email protected] (G.T.O.), [email protected] (J.-H.C.), [email protected] (C.C.) http://dx.doi.org/10.1016/j.cmet.2016.11.008
(Cell Metabolism 23, 852–866; May 10, 2016) In the preparation and publication of this article, one figure showing the efficacy of prolonged plasmacytoid dendritic cell (pDC) depletion in BDCA2-DTR bone marrow (BM) reconstituted mice was omitted from the manuscript due to author oversight. To selectively deplete pDCs in our athero-prone mouse model, we lethally irradiated Ldlr / mice and reconstituted with BM cells of BDCA2-DTR mice. After 8 weeks, the reconstituted Ldlr / mice were fed Western-type diet (WD) and injected with PBS alone or with diphtheria toxin (DT) for the indicated time period as described in the original paper’s Figure 4H. Reconstituted mice showed prolonged pDC depletion in spleen by DT injection for 12 weeks (see new Figures 1A and 1B). We also depleted pDCs from the reconstituted mice to test the role of pDCs in already-established atherosclerosis by feeding them WD for 12 weeks but injecting DT only for the last 6 weeks, and observed successful depletion of splenic pDCs (see new Figures 1C and 1D). This additional figure does not affect the conclusion of our paper, and we apologize for any inconvenience.
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Figure 1. Prolonged Depletion of Splenic pDCs in Ldlr–/– Mice Reconstituted with Bone Marrow Cells of BDCA2-DTR Mice (A–D) Lethally irradiated Ldlr / mice reconstituted with BM cells of BDCA2-DTR mice were fed WD and injected i.p. with PBS or 0.5 mg DT weekly for 12 weeks (A and B), or were fed WD for 12 weeks and injected i.p. with PBS or 0.5 mg DT weekly only for the last 6 weeks (C and D). (A) Enumeration of pDCs in spleen (per 1 3 106 CD45+ cells). Bars indicate mean; n R 10. (B) Representative FACS plots of pDCs in spleen. Numbers in plots indicate the percentages of pDCs. (C) Enumeration of pDCs in spleen (per 1 3 106 CD45+ cells). Bars indicate mean; n R 8. (D) Representative FACS plots of pDCs in spleen. Numbers in plots indicate the percentages of pDCs.
886 Cell Metabolism 24, 886–888, December 13, 2016 ª 2016 Elsevier Inc.
Corrections Indoleamine 2,3-Dioxygenase-Expressing Aortic Plasmacytoid Dendritic Cells Protect against Atherosclerosis by Induction of Regulatory T Cells Tae Jin Yun, Jun Seong Lee, Kawthar Machmach, Dahee Shim, Junhee Choi, Young Jin Wi, Hyung Seok Jang, In-Hyuk Jung, Kyeongdae Kim, Won Kee Yoon, Mohammad Alam Miah, Bin Li, Jinsam Chang, Mariana G. Bego, Tram N.Q. Pham, Jakob Loschko, Jo¨rg Hermann Fritz, Anne B. Krug, Seung-Pyo Lee, Tibor Keler, Jean V. Guimond, Elie Haddad, Eric A. Cohen, Martin G. Sirois, Ismail El-Hamamsy, Marco Colonna, Goo Taeg Oh,* Jae-Hoon Choi,* and Cheolho Cheong* *Correspondence: [email protected] (G.T.O.), [email protected] (J.-H.C.), [email protected] (C.C.) http://dx.doi.org/10.1016/j.cmet.2016.11.008
(Cell Metabolism 23, 852–866; May 10, 2016) In the preparation and publication of this article, one figure showing the efficacy of prolonged plasmacytoid dendritic cell (pDC) depletion in BDCA2-DTR bone marrow (BM) reconstituted mice was omitted from the manuscript due to author oversight. To selectively deplete pDCs in our athero-prone mouse model, we lethally irradiated Ldlr / mice and reconstituted with BM cells of BDCA2-DTR mice. After 8 weeks, the reconstituted Ldlr / mice were fed Western-type diet (WD) and injected with PBS alone or with diphtheria toxin (DT) for the indicated time period as described in the original paper’s Figure 4H. Reconstituted mice showed prolonged pDC depletion in spleen by DT injection for 12 weeks (see new Figures 1A and 1B). We also depleted pDCs from the reconstituted mice to test the role of pDCs in already-established atherosclerosis by feeding them WD for 12 weeks but injecting DT only for the last 6 weeks, and observed successful depletion of splenic pDCs (see new Figures 1C and 1D). This additional figure does not affect the conclusion of our paper, and we apologize for any inconvenience.
A
B
C
D
Figure 1. Prolonged Depletion of Splenic pDCs in Ldlr–/– Mice Reconstituted with Bone Marrow Cells of BDCA2-DTR Mice (A–D) Lethally irradiated Ldlr / mice reconstituted with BM cells of BDCA2-DTR mice were fed WD and injected i.p. with PBS or 0.5 mg DT weekly for 12 weeks (A and B), or were fed WD for 12 weeks and injected i.p. with PBS or 0.5 mg DT weekly only for the last 6 weeks (C and D). (A) Enumeration of pDCs in spleen (per 1 3 106 CD45+ cells). Bars indicate mean; n R 10. (B) Representative FACS plots of pDCs in spleen. Numbers in plots indicate the percentages of pDCs. (C) Enumeration of pDCs in spleen (per 1 3 106 CD45+ cells). Bars indicate mean; n R 8. (D) Representative FACS plots of pDCs in spleen. Numbers in plots indicate the percentages of pDCs.
886 Cell Metabolism 24, 886–888, December 13, 2016 ª 2016 Elsevier Inc.