- Email: [email protected]
MRI findings in brain tumors in dogs
S46
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
and the other, a developed technology, the treatment with a synthetic analogue of gonadotropin releasing hormone (GnRH) combined with a dopamine receptor antagonist. In a private unit for intensive common carp rearing there were used comparatively the methods mentioned above, trying the cell maturation at different temperatures, 20.5 ◦ C beside 25 ◦ C and different doses of hormones. Experiments on common carp were carried out in the breeding season of 2016. When the natural hormone was injected, the very fast decreasing of plasmatic concentration was noted, consequently, the ovulation induced by analogue hormones was longer, lasting 48 h, beside 12 h in the case of the natural hormone. At 20.5 ◦ C the ovulation time increases with approximately 5 h beside the one at 25 ◦ C. It should be conclude that the latency period between treatment and ovulation was shorter and the time of ovulation was more predictable after the pituitary treatment. Meanwhile, the synthetic analogue is cheaper than pituitary due to the fact that pituitary sampling involves fish slaughtering. http://dx.doi.org/10.1016/j.jbiotec.2017.06.951 The origin of Turkish native chickens, Denizli and Gerze Meydan 1,∗ ,
Hasan Weigend 3
Mehmet Ali
Yildiz 2 ,
Steffen
1
Akdeniz University, Faculty of Agriculture, Department of Agricultural Biotechnology, Antalya, Turkey 2 Ankara University, Faculty of Agriculture, Department of Animal Sciences, Ankara, Turkey 3 Friedrich-Loeffler-Institut, Institute of Farm Animal Genetics, Mariensee, Neustadt, Germany E-mail address: [email protected] (H. Meydan). To assess genetic diversity and maternal origin of Turkish native chicken breeds, we analyzed the mtDNA D-loop sequences of Denizli and from Gerze native chicken breeds, together with the available reference sequences of G. gallus gallus in GenBank. The higher haplotype diversity was estimated for Denizli breed than Gerze breed. Only one clade or haplogroup (clade E) was found in Turkish breeds. Clade E haplotypes, which are quite common in the world poultry breeds, were observed in Denizli and Gerze populations with 4 different haplotypes, of which TRE1 was a major haplotype with the frequency of 87% (47/54) across two breeds. These results suggest that Denizli and Gerze chickens originated from the same region, the Indian subcontinent. Our results will provide reliable basic information for mtDNA haplotypes of Turkish native chickens and for studying the origin of domestic chickens. http://dx.doi.org/10.1016/j.jbiotec.2017.06.952 Association between the MspI polymorphism in growth hormone gene and milk yield in holstein cows Emine Sahin 1,∗ , Murat Soner Balcioglu 2 , Taki Karsli 2 , Eymen Demir 2 1
Department of Animal Production, Korkuteli Vocational School, Akdeniz University, Antalya, Turkey 2 Department of Animal Science, Faculty of Agriculture, Akdeniz University, Antalya, Turkey E-mail address: [email protected] (E. Sahin).
The aim of this study was to determine relationships between the bovine growth hormone (bGH) gene polymorphism and milk yield in Holstein cows raised in Antalya, Turkey. In order to determine the bGH-MspI polymorphism, polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) method was performed. For bGH gen, the fragment 891 bp was amplified and two types of alleles as C and D were observed by RFLP method. In the study, the frequencies of C and D alleles were determined as 0.8729 and 0.1271, respectively. In addition, the genotype frequencies for CC, CD and DD were calculated as 0.7627, 0.2203 and 0.0169, respectively. In bGH locus, a significant deviation from the Hardy-Weinberg equilibrium was not determined in the current population. According to the results of analysis, there was no significant relationship between the genotypes of bGH-MspI polymorphism with milk yield in Holstein cows. http://dx.doi.org/10.1016/j.jbiotec.2017.06.953 Thelazia callipaeda infection in dogs from Bucharest and surrounding areas Poliana Tudor ∗ , Iuliana Ionascu Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (P. Tudor). Thelazia callipaeda is a nematode that usually parasites the conjunctival sac of domestic and wildlife animals and as in humans. Both the adults and larvae stages are involved in the pathogenesis of the disease, and the clinical picture of the disease is characterized by a wide range of ocular signs, from subclinical to mild or severe. This study presents a case series of T. callipaeda infection from dogs living in Bucharest and surrounding areas. During 2016, in the Clinic of the Faculty of Veterinary Medicine Bucharest were diagnosed 8 cases with ocular canine telaziosis, animals with age between 1 and 10 years, 3 males and 5 females. Infection was unilateral in 2 cases and bilateral in 6 cases. Clinical signs were represented by conjunctivitis, eye discharge, pruritus, blepharospasm and corneal ulcer. After instillation of anesthetic eye drops, parasitic worms were harvested using sterile cotton swabs, and morphologically identified as T. callipaeda. Each animal was treated according to the clinical signs and followed till the remission of the signs. This report shows a higher spread of this parasite in the studied area than previously reported. Moreover, its zoonotic character requires better information of the owners, physicians and veterinarians. http://dx.doi.org/10.1016/j.jbiotec.2017.06.954 MRI findings in brain tumors in dogs Alexandru Gabriel Neagu ∗ , Mihai Savescu, Gabriel Predoi, Catalin Micsa, Niculae Tudor, Constantin Vlagioiu Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (A.G. Neagu). Primary or secondary brain tumors are often diagnosed in dogs. With the introduction of magnetic resonance imaging, their prevalence has increased considerably, making it easier to detect. The aim of this study was to evaluate MRI images in different types of brain tumors in dogs, confirmed by histopathology. Medical records of 28 dogs of different breeds, with ages between two to nine years, were studied, the criteria on which they were selected was the MRI and the histopathologic examination. MRI examination was performed using a 0.3 Tesla magnetic resonance imaging. The protocols used were T1 and T2 FSE in all three planes, in some cases T1 sequences
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
with contrast agent was acquired. Dogs were positioned in sternoabdominal recumbency and inhalatory anesthesia consisted in premedication with one of the following substances: Diazepam, Butorphanol, Domitor; induction with Propofol and maintenance with Isoflurane. Following the evaluation of the acquired images have been identified these types of lesions: the choroid plexus tumors (32.14%), pituitary adenoma (17.86%), glial-type tumors, astrocytoma (21.43%) and oligodendroglioma (28.57%). The results of the study indicated that MRI examination is useful in detecting features of brain tumors in dogs. In addition, histopathological examination is necessary for a diagnosis of certainty. http://dx.doi.org/10.1016/j.jbiotec.2017.06.955 MRI findings in intervertebral disc disease on thoracolumbar spine in dogs Mihai Savescu 1,∗ , Alexandru Gabriel Neagu 1 , Constantin Vlagioiu 1 , Niculae Tudor 1 , Gabriel Predoi 1 , Iulian Raus 2 1
Faculty of Veterinary Medicine, Bucharest, Romania 2 Emergency Hospital Dr. Constantin Papilian, Cluj-Napoca, Romania E-mail address: [email protected] (M. Savescu). Intervertebral disc disease is a common cause of back pain and neurological deficits in dogs, affecting more frequently chondrodystrophic and geriatrics dogs. The intervertebral disc degeneration can lead to a number of diseases like disc hernia, degenerative lombosacral stenosis and cervical spondylomyelopathy. The aim of this study was to evaluate the intervertebral disc in the lumbar and thoracic segment, as well as the anatomical distribution of disc hernia in dogs. Therefore, medical records from the Imaging Center of the Faculty of Veterinary Medicine of Bucharest were reviewed in order to identify dogs diagnosed with a thoracolombar herniated disc using magnetic resonance. Twenty-one dogs (12M and 9F) of different breeds, between two and nine years old, with clinical, neurological and imaging examination results, were included in the study. The clinical sings were lethargy, pain, ataxia, paraparesis and paraplegia. The imaging examination revealed intervertebral disc degeneration in a total of 52 disc, with the highest prevalence in L3-L4 (10.6%) and L6-L7 (9.0%), respectively. From the total affected disc, 10.6% showed Hansen type I hernia and 7.5% revealed Hansen type II. MRI is an essential tool for the detection of early disc degenerative phenomenon in dogs. http://dx.doi.org/10.1016/j.jbiotec.2017.06.956 Midazolam-fentanyl-ketamine infusion provides myorelaxation for laparoscopic abdominal procedures
i.m.), followed by induction with propofol (4 mg/kg i.v.) and maintained with isoflurane. Pigs were randomly allocated to one of the following groups: MFK group (receiving midazolam 0.2 mg/kg/h, fentanyl 10 g/kg/h and ketamine 10 g/kg/min) or KL group (receiving ketamine 10 g/kg/min and lidocaine 30 g/kg/min). All patients were monitored (ECG, blood pressure, pulsoximetry) during the procedures and anesthesia. The midazolam-fentanylketamine combination had the greatest success rate regarding analgesia and muscle relaxation (mean heart rate 80 bpm, compared to 115 bpm for the KL group). The medium concentration of isoflurane in the MFK group was 1%, whereas for the second group the value was 2.5%. In summary, MFK provides a safe and effective mean of sedation and myorelaxation for different abdominal laparoscopic procedures. This drug combination can provide minimal cardiovascular effects, mild reaction to injection and a favorable recovery. http://dx.doi.org/10.1016/j.jbiotec.2017.06.957 Bronchoalveolar lavage findings in dogs with pulmonary lesions Dorin Togoe ∗ , Kassem Houshaimy, Alexandru Gabriel Neagu, Catalin Micsa Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (D. Togoe). Diagnostic techniques used in small animals to evaluate lungs diseases are limited because abnormalities detected radiographically are rarely specific for a single disease process. Bronchoalveolar lavage (BAL) is a minimally invasive technique utilized to sample lower bronchi and alveolar spaces. Study was performed on 21 dogs with neoplastic disease history and radiographic pulmonary lesion, that we are divided in three groups: interstitial pattern (8), single well-defined lesion (4) and multifocal lesions in lung parenchyma (9). BAL specimens were analyzed cytological and microbiological. As a result of the cytological analysis the animals were divided in two groups: neoplastic lesions (8) and inflammatory lung disease (13). Neoplastic lesions were diagnosed as: carcinoma (4), lymphoma (3) and fibrosarcoma (1). Inflammatory diseases have been represented by: interstitial pneumonia (8), bronchopneumonia (3) and allergic reactions (2). Pathogens involved, were represented by Klebsiella spp (3), Pseudomonas spp (2), Escherichia coli (2), Corynebacterium spp (3), Mycoplasma spp (1). Allergic reactions were determined by microfilaria. In conclusion, BAL is a useful tool to provide first cytologic information in dogs with lung diseases. Further study will be necessary to establish the role of BAL in clinical veterinary medicine and standardization of technique for optimum results. http://dx.doi.org/10.1016/j.jbiotec.2017.06.958
Catalin Micsa ∗ , Dorin Togoe, Emilia Balint, Alexandru Gabriel Neagu, Kassem Houshaimy, Alexandru Sonea
The relationship between shelter management and fattening cattle welfare
Faculty of Veterinary Medicine, Bucharest, Romania
Ioana Cristina Andronie 1,∗ , Monica Parvu 1 , Cristian Mortasivu 1 , Viorel Andronie 1 , Elena Mitranescu 2
E-mail address: [email protected] (C. Micsa). Laparoscopic surgery gained a lot of field in human and veterinary medicine, successfully replacing many invasive surgical techniques. The goal of this study was to determine a successful anaesthesia which can provide sufficient analgesia, narcosis and myorelaxation for laparoscopic abdominal procedures. Twenty swines were included in this study (mean weight of 30 kg) and were premedicated with ketamine (20 mg/kg i.m.) and xylazine (2 mg/kg
S47
1
Spiru Haret University, Faculty of Veterinary Medicine, Bucharest, Romania 2 University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (I.C. Andronie).
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
and the other, a developed technology, the treatment with a synthetic analogue of gonadotropin releasing hormone (GnRH) combined with a dopamine receptor antagonist. In a private unit for intensive common carp rearing there were used comparatively the methods mentioned above, trying the cell maturation at different temperatures, 20.5 ◦ C beside 25 ◦ C and different doses of hormones. Experiments on common carp were carried out in the breeding season of 2016. When the natural hormone was injected, the very fast decreasing of plasmatic concentration was noted, consequently, the ovulation induced by analogue hormones was longer, lasting 48 h, beside 12 h in the case of the natural hormone. At 20.5 ◦ C the ovulation time increases with approximately 5 h beside the one at 25 ◦ C. It should be conclude that the latency period between treatment and ovulation was shorter and the time of ovulation was more predictable after the pituitary treatment. Meanwhile, the synthetic analogue is cheaper than pituitary due to the fact that pituitary sampling involves fish slaughtering. http://dx.doi.org/10.1016/j.jbiotec.2017.06.951 The origin of Turkish native chickens, Denizli and Gerze Meydan 1,∗ ,
Hasan Weigend 3
Mehmet Ali
Yildiz 2 ,
Steffen
1
Akdeniz University, Faculty of Agriculture, Department of Agricultural Biotechnology, Antalya, Turkey 2 Ankara University, Faculty of Agriculture, Department of Animal Sciences, Ankara, Turkey 3 Friedrich-Loeffler-Institut, Institute of Farm Animal Genetics, Mariensee, Neustadt, Germany E-mail address: [email protected] (H. Meydan). To assess genetic diversity and maternal origin of Turkish native chicken breeds, we analyzed the mtDNA D-loop sequences of Denizli and from Gerze native chicken breeds, together with the available reference sequences of G. gallus gallus in GenBank. The higher haplotype diversity was estimated for Denizli breed than Gerze breed. Only one clade or haplogroup (clade E) was found in Turkish breeds. Clade E haplotypes, which are quite common in the world poultry breeds, were observed in Denizli and Gerze populations with 4 different haplotypes, of which TRE1 was a major haplotype with the frequency of 87% (47/54) across two breeds. These results suggest that Denizli and Gerze chickens originated from the same region, the Indian subcontinent. Our results will provide reliable basic information for mtDNA haplotypes of Turkish native chickens and for studying the origin of domestic chickens. http://dx.doi.org/10.1016/j.jbiotec.2017.06.952 Association between the MspI polymorphism in growth hormone gene and milk yield in holstein cows Emine Sahin 1,∗ , Murat Soner Balcioglu 2 , Taki Karsli 2 , Eymen Demir 2 1
Department of Animal Production, Korkuteli Vocational School, Akdeniz University, Antalya, Turkey 2 Department of Animal Science, Faculty of Agriculture, Akdeniz University, Antalya, Turkey E-mail address: [email protected] (E. Sahin).
The aim of this study was to determine relationships between the bovine growth hormone (bGH) gene polymorphism and milk yield in Holstein cows raised in Antalya, Turkey. In order to determine the bGH-MspI polymorphism, polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) method was performed. For bGH gen, the fragment 891 bp was amplified and two types of alleles as C and D were observed by RFLP method. In the study, the frequencies of C and D alleles were determined as 0.8729 and 0.1271, respectively. In addition, the genotype frequencies for CC, CD and DD were calculated as 0.7627, 0.2203 and 0.0169, respectively. In bGH locus, a significant deviation from the Hardy-Weinberg equilibrium was not determined in the current population. According to the results of analysis, there was no significant relationship between the genotypes of bGH-MspI polymorphism with milk yield in Holstein cows. http://dx.doi.org/10.1016/j.jbiotec.2017.06.953 Thelazia callipaeda infection in dogs from Bucharest and surrounding areas Poliana Tudor ∗ , Iuliana Ionascu Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (P. Tudor). Thelazia callipaeda is a nematode that usually parasites the conjunctival sac of domestic and wildlife animals and as in humans. Both the adults and larvae stages are involved in the pathogenesis of the disease, and the clinical picture of the disease is characterized by a wide range of ocular signs, from subclinical to mild or severe. This study presents a case series of T. callipaeda infection from dogs living in Bucharest and surrounding areas. During 2016, in the Clinic of the Faculty of Veterinary Medicine Bucharest were diagnosed 8 cases with ocular canine telaziosis, animals with age between 1 and 10 years, 3 males and 5 females. Infection was unilateral in 2 cases and bilateral in 6 cases. Clinical signs were represented by conjunctivitis, eye discharge, pruritus, blepharospasm and corneal ulcer. After instillation of anesthetic eye drops, parasitic worms were harvested using sterile cotton swabs, and morphologically identified as T. callipaeda. Each animal was treated according to the clinical signs and followed till the remission of the signs. This report shows a higher spread of this parasite in the studied area than previously reported. Moreover, its zoonotic character requires better information of the owners, physicians and veterinarians. http://dx.doi.org/10.1016/j.jbiotec.2017.06.954 MRI findings in brain tumors in dogs Alexandru Gabriel Neagu ∗ , Mihai Savescu, Gabriel Predoi, Catalin Micsa, Niculae Tudor, Constantin Vlagioiu Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (A.G. Neagu). Primary or secondary brain tumors are often diagnosed in dogs. With the introduction of magnetic resonance imaging, their prevalence has increased considerably, making it easier to detect. The aim of this study was to evaluate MRI images in different types of brain tumors in dogs, confirmed by histopathology. Medical records of 28 dogs of different breeds, with ages between two to nine years, were studied, the criteria on which they were selected was the MRI and the histopathologic examination. MRI examination was performed using a 0.3 Tesla magnetic resonance imaging. The protocols used were T1 and T2 FSE in all three planes, in some cases T1 sequences
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
with contrast agent was acquired. Dogs were positioned in sternoabdominal recumbency and inhalatory anesthesia consisted in premedication with one of the following substances: Diazepam, Butorphanol, Domitor; induction with Propofol and maintenance with Isoflurane. Following the evaluation of the acquired images have been identified these types of lesions: the choroid plexus tumors (32.14%), pituitary adenoma (17.86%), glial-type tumors, astrocytoma (21.43%) and oligodendroglioma (28.57%). The results of the study indicated that MRI examination is useful in detecting features of brain tumors in dogs. In addition, histopathological examination is necessary for a diagnosis of certainty. http://dx.doi.org/10.1016/j.jbiotec.2017.06.955 MRI findings in intervertebral disc disease on thoracolumbar spine in dogs Mihai Savescu 1,∗ , Alexandru Gabriel Neagu 1 , Constantin Vlagioiu 1 , Niculae Tudor 1 , Gabriel Predoi 1 , Iulian Raus 2 1
Faculty of Veterinary Medicine, Bucharest, Romania 2 Emergency Hospital Dr. Constantin Papilian, Cluj-Napoca, Romania E-mail address: [email protected] (M. Savescu). Intervertebral disc disease is a common cause of back pain and neurological deficits in dogs, affecting more frequently chondrodystrophic and geriatrics dogs. The intervertebral disc degeneration can lead to a number of diseases like disc hernia, degenerative lombosacral stenosis and cervical spondylomyelopathy. The aim of this study was to evaluate the intervertebral disc in the lumbar and thoracic segment, as well as the anatomical distribution of disc hernia in dogs. Therefore, medical records from the Imaging Center of the Faculty of Veterinary Medicine of Bucharest were reviewed in order to identify dogs diagnosed with a thoracolombar herniated disc using magnetic resonance. Twenty-one dogs (12M and 9F) of different breeds, between two and nine years old, with clinical, neurological and imaging examination results, were included in the study. The clinical sings were lethargy, pain, ataxia, paraparesis and paraplegia. The imaging examination revealed intervertebral disc degeneration in a total of 52 disc, with the highest prevalence in L3-L4 (10.6%) and L6-L7 (9.0%), respectively. From the total affected disc, 10.6% showed Hansen type I hernia and 7.5% revealed Hansen type II. MRI is an essential tool for the detection of early disc degenerative phenomenon in dogs. http://dx.doi.org/10.1016/j.jbiotec.2017.06.956 Midazolam-fentanyl-ketamine infusion provides myorelaxation for laparoscopic abdominal procedures
i.m.), followed by induction with propofol (4 mg/kg i.v.) and maintained with isoflurane. Pigs were randomly allocated to one of the following groups: MFK group (receiving midazolam 0.2 mg/kg/h, fentanyl 10 g/kg/h and ketamine 10 g/kg/min) or KL group (receiving ketamine 10 g/kg/min and lidocaine 30 g/kg/min). All patients were monitored (ECG, blood pressure, pulsoximetry) during the procedures and anesthesia. The midazolam-fentanylketamine combination had the greatest success rate regarding analgesia and muscle relaxation (mean heart rate 80 bpm, compared to 115 bpm for the KL group). The medium concentration of isoflurane in the MFK group was 1%, whereas for the second group the value was 2.5%. In summary, MFK provides a safe and effective mean of sedation and myorelaxation for different abdominal laparoscopic procedures. This drug combination can provide minimal cardiovascular effects, mild reaction to injection and a favorable recovery. http://dx.doi.org/10.1016/j.jbiotec.2017.06.957 Bronchoalveolar lavage findings in dogs with pulmonary lesions Dorin Togoe ∗ , Kassem Houshaimy, Alexandru Gabriel Neagu, Catalin Micsa Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (D. Togoe). Diagnostic techniques used in small animals to evaluate lungs diseases are limited because abnormalities detected radiographically are rarely specific for a single disease process. Bronchoalveolar lavage (BAL) is a minimally invasive technique utilized to sample lower bronchi and alveolar spaces. Study was performed on 21 dogs with neoplastic disease history and radiographic pulmonary lesion, that we are divided in three groups: interstitial pattern (8), single well-defined lesion (4) and multifocal lesions in lung parenchyma (9). BAL specimens were analyzed cytological and microbiological. As a result of the cytological analysis the animals were divided in two groups: neoplastic lesions (8) and inflammatory lung disease (13). Neoplastic lesions were diagnosed as: carcinoma (4), lymphoma (3) and fibrosarcoma (1). Inflammatory diseases have been represented by: interstitial pneumonia (8), bronchopneumonia (3) and allergic reactions (2). Pathogens involved, were represented by Klebsiella spp (3), Pseudomonas spp (2), Escherichia coli (2), Corynebacterium spp (3), Mycoplasma spp (1). Allergic reactions were determined by microfilaria. In conclusion, BAL is a useful tool to provide first cytologic information in dogs with lung diseases. Further study will be necessary to establish the role of BAL in clinical veterinary medicine and standardization of technique for optimum results. http://dx.doi.org/10.1016/j.jbiotec.2017.06.958
Catalin Micsa ∗ , Dorin Togoe, Emilia Balint, Alexandru Gabriel Neagu, Kassem Houshaimy, Alexandru Sonea
The relationship between shelter management and fattening cattle welfare
Faculty of Veterinary Medicine, Bucharest, Romania
Ioana Cristina Andronie 1,∗ , Monica Parvu 1 , Cristian Mortasivu 1 , Viorel Andronie 1 , Elena Mitranescu 2
E-mail address: [email protected] (C. Micsa). Laparoscopic surgery gained a lot of field in human and veterinary medicine, successfully replacing many invasive surgical techniques. The goal of this study was to determine a successful anaesthesia which can provide sufficient analgesia, narcosis and myorelaxation for laparoscopic abdominal procedures. Twenty swines were included in this study (mean weight of 30 kg) and were premedicated with ketamine (20 mg/kg i.m.) and xylazine (2 mg/kg
S47
1
Spiru Haret University, Faculty of Veterinary Medicine, Bucharest, Romania 2 University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (I.C. Andronie).