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Preparation and properties of novel microporous hydrogels with poly(ethylene glycol) dimethacrylate and carboxylated carbon nanotubes
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Abstracts / Journal of Controlled Release 213 (2015) e8–e152
Fig. 1. (a) Schematic representation of temperature-responsive nanocarriers for intracellular uptake. (b) Fluorescence microscopic images of HeLa cells at 37 °C.
Keywords: temperature-responsive, nanocarrier, intracellular uptake
amphiphilic,
core–shell
References [1] P. Li, Y.S. Siu, K.M. HO, W. Li, Amphiphilic core–shell nanoparticles containing hairy polyethyleneimine shells as effective nanocarriers for gene delivery,book chapter, Selected Topics in Nanomedicine, World Scientific Publishing, 2014, ISBN 978-981-4472-85-2. [2] H. Mimi, K.M. Ho, Y.S. Siu, A. Wu, P. Li, Polyethyleneiminebased core–shell nanogels: a promising siRNA carrier for argininosuccinate synthetase mRNA knockdown in HeLa Cells, J. Control. Release 158 (2012) 123–130.
doi:10.1016/j.jconrel.2015.05.142
Preparation and properties of novel microporous hydrogels with poly(ethylene glycol) dimethacrylate and carboxylated carbon nanotubes Pei Wanga, Shuai Wangb, LianLiua, Yuan Liua, Ming Zhanga Department of Materials Science and Engineering, Dalian Maritime University, Dalian 116026, China b First Affiliated Hospital of Dalian Medical University, Dalian 116011, China E-mail address: [email protected] (P. Wang).
a
Poly(ethylene glycol) (PEG) hydrogels are widely studied and frequently used biomaterials [1,2]. A significant challenge to hydrogels is to control their shape and size. Our research interests were the design of novel microscale hybrid hydrogel networks under bio-friendly conditions. In this work, hydrogels were fabricated by free-radical redox polymerization with PEG dimethacrylate (PEGDMA) (200 g/mol) and 2-hydroxyethyl methacrylate (HEMA) using ammonium persulfate (APS) and N, N, N′, N′-tetramethylethylene diamine (TEMED) as initiators and N, N′-methylene bisacrylamide (BIS) as cross-linker (Fig. 1). Carboxylated carbon nanotubes (CNTs) were also added to the copolymer system to improve the swelling and mechanical properties. Cross-linked hydrogel samples were obtained after freeze-drying. We studied the water absorption rate of the samples with different mass contents of HEMA and BIS in water. The different aperture and crosslinking density had great influences on the ability of water uptake. It should be pointed out that hydrogels with nano-filler had a much higher water absorption rate. In particular, the equilibrium value was as high as 537% with 0.3 wt.% CNTs. SEM images (SUPRA 55 SAPPHIRE, ZEISS) of the samples exhibited a uniform microparticular morphology with a micron pore size (mesh size b1 μm). Furthermore, the microparticular morphology can be modified by changing the amount of crosslinker and nanofillers. The obtained hydrogels with appropriate crosslink density may be further exploited for biomedical applications, such as three dimensional tissue scaffolds.
Fig. 1. Schematic illustration of PEGDMA-co-HEMA/CNTs hybrid hydrogels and SEM image.
Keywords: cross-linking hydrogel, PEGDMA, free-radical redox polymerization, carbon nanotubes (CNT) Acknowledgments This work was supported by the Natural Science Foundation of Liaoning Province (2014025011) and Fundamental Research Funds for the Central Universities (3132014076, 3132014323). References [1] N. Peppas, J.Z. Hilt, A. Khademhosseini, R. Langer, Hydrogels in biology and medicine: from molecular principles to bionanotechnology, Adv. Mater. 18 (2006) 1345–1360. [2] J. Yeh, Y.b. Ling, J.M. Karpd, J. Gantze, A. Chandawarkar, G. Eng, J. Blumling, R. Langer, A. Khademhosseini, Micromolding of shape-controlled, harvestable cell-laden hydrogels, Biomaterials 27 (2006) 5391–5398.
doi:10.1016/j.jconrel.2015.05.143
Folate-conjugated biodegradable core cross-linked polyphosphoester micelles for targeted and pH-triggered drug delivery Jian Hu, Jinlin He, Mingzu Zhang, Peihong Ni* College of Chemistry, Chemical Engineering and Materials Science, Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Soochow University, Suzhou 215123, China ⁎Corresponding author. E-mail address: [email protected] (P. Ni). Polymeric micelles, self-assembled from amphiphilic block copolymers in aqueous medium with a nanoscopic size range, have been regarded as one of the most commonly used anti-cancer drug delivery systems. The applications of polymeric micelles, however, have been hampered by their poor in vivo stability, which leads to micellar dissociation and premature release of encapsulated drugs [1]. Recent interests in preventing their dissociation in the bloodstream upon intravenous administration have led to increasing stability of polymeric micelles. One of the important strategies is cross-linking of the micellar core or shell [1,2]. On the other hand, with the rapid development of “smart” nanostructured materials, stimuli-responsive polymers with cleavable linkages have attracted increasing attention. pH-Responsive polymers are the most frequently studied in triggered drug delivery system since the pH values in different tissues and cellular compartments vary tremendously [3]. It will be highly beneficial if the anti-cancer drugs are delivered by a system that are sensitive to the signal caused by the
Abstracts / Journal of Controlled Release 213 (2015) e8–e152
Fig. 1. (a) Schematic representation of temperature-responsive nanocarriers for intracellular uptake. (b) Fluorescence microscopic images of HeLa cells at 37 °C.
Keywords: temperature-responsive, nanocarrier, intracellular uptake
amphiphilic,
core–shell
References [1] P. Li, Y.S. Siu, K.M. HO, W. Li, Amphiphilic core–shell nanoparticles containing hairy polyethyleneimine shells as effective nanocarriers for gene delivery,book chapter, Selected Topics in Nanomedicine, World Scientific Publishing, 2014, ISBN 978-981-4472-85-2. [2] H. Mimi, K.M. Ho, Y.S. Siu, A. Wu, P. Li, Polyethyleneiminebased core–shell nanogels: a promising siRNA carrier for argininosuccinate synthetase mRNA knockdown in HeLa Cells, J. Control. Release 158 (2012) 123–130.
doi:10.1016/j.jconrel.2015.05.142
Preparation and properties of novel microporous hydrogels with poly(ethylene glycol) dimethacrylate and carboxylated carbon nanotubes Pei Wanga, Shuai Wangb, LianLiua, Yuan Liua, Ming Zhanga Department of Materials Science and Engineering, Dalian Maritime University, Dalian 116026, China b First Affiliated Hospital of Dalian Medical University, Dalian 116011, China E-mail address: [email protected] (P. Wang).
a
Poly(ethylene glycol) (PEG) hydrogels are widely studied and frequently used biomaterials [1,2]. A significant challenge to hydrogels is to control their shape and size. Our research interests were the design of novel microscale hybrid hydrogel networks under bio-friendly conditions. In this work, hydrogels were fabricated by free-radical redox polymerization with PEG dimethacrylate (PEGDMA) (200 g/mol) and 2-hydroxyethyl methacrylate (HEMA) using ammonium persulfate (APS) and N, N, N′, N′-tetramethylethylene diamine (TEMED) as initiators and N, N′-methylene bisacrylamide (BIS) as cross-linker (Fig. 1). Carboxylated carbon nanotubes (CNTs) were also added to the copolymer system to improve the swelling and mechanical properties. Cross-linked hydrogel samples were obtained after freeze-drying. We studied the water absorption rate of the samples with different mass contents of HEMA and BIS in water. The different aperture and crosslinking density had great influences on the ability of water uptake. It should be pointed out that hydrogels with nano-filler had a much higher water absorption rate. In particular, the equilibrium value was as high as 537% with 0.3 wt.% CNTs. SEM images (SUPRA 55 SAPPHIRE, ZEISS) of the samples exhibited a uniform microparticular morphology with a micron pore size (mesh size b1 μm). Furthermore, the microparticular morphology can be modified by changing the amount of crosslinker and nanofillers. The obtained hydrogels with appropriate crosslink density may be further exploited for biomedical applications, such as three dimensional tissue scaffolds.
Fig. 1. Schematic illustration of PEGDMA-co-HEMA/CNTs hybrid hydrogels and SEM image.
Keywords: cross-linking hydrogel, PEGDMA, free-radical redox polymerization, carbon nanotubes (CNT) Acknowledgments This work was supported by the Natural Science Foundation of Liaoning Province (2014025011) and Fundamental Research Funds for the Central Universities (3132014076, 3132014323). References [1] N. Peppas, J.Z. Hilt, A. Khademhosseini, R. Langer, Hydrogels in biology and medicine: from molecular principles to bionanotechnology, Adv. Mater. 18 (2006) 1345–1360. [2] J. Yeh, Y.b. Ling, J.M. Karpd, J. Gantze, A. Chandawarkar, G. Eng, J. Blumling, R. Langer, A. Khademhosseini, Micromolding of shape-controlled, harvestable cell-laden hydrogels, Biomaterials 27 (2006) 5391–5398.
doi:10.1016/j.jconrel.2015.05.143
Folate-conjugated biodegradable core cross-linked polyphosphoester micelles for targeted and pH-triggered drug delivery Jian Hu, Jinlin He, Mingzu Zhang, Peihong Ni* College of Chemistry, Chemical Engineering and Materials Science, Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Soochow University, Suzhou 215123, China ⁎Corresponding author. E-mail address: [email protected] (P. Ni). Polymeric micelles, self-assembled from amphiphilic block copolymers in aqueous medium with a nanoscopic size range, have been regarded as one of the most commonly used anti-cancer drug delivery systems. The applications of polymeric micelles, however, have been hampered by their poor in vivo stability, which leads to micellar dissociation and premature release of encapsulated drugs [1]. Recent interests in preventing their dissociation in the bloodstream upon intravenous administration have led to increasing stability of polymeric micelles. One of the important strategies is cross-linking of the micellar core or shell [1,2]. On the other hand, with the rapid development of “smart” nanostructured materials, stimuli-responsive polymers with cleavable linkages have attracted increasing attention. pH-Responsive polymers are the most frequently studied in triggered drug delivery system since the pH values in different tissues and cellular compartments vary tremendously [3]. It will be highly beneficial if the anti-cancer drugs are delivered by a system that are sensitive to the signal caused by the