SCIENTIFIC EDITION methy1)sulfanilamidomethane with acetic anhydride and glacial acetic acid gave.a penta-acetyl derivative which melted at 193-195". Tri(hydroxymethy1)sulfanilamidomethane was tested bacteriostatically, in uitro, against four microorganisms, Proteus uulgaris, Escherichiu coli, Staphylococcus aureus, and Streptococcus hemolyticus. A simple qualitative method was used, in which three tubes, each containing 5 cc. of beef infusion, were inoculated with one of the organisms named above. To one of the tubes 25 mg. of tri(hydroxymethy1)sulfanilamidomethane was added, to another 25 mg. of sulfathiazole sodium, the third tube being used as a control. Tri (hydroxymethy1)sulfanilamidomethane showed inhibition of growth on Escherichiu coli, Proteus vulgaris, and Streptococcus hemolyticus, but in no case was the inhibition comparable t o that of sulfathiazole sodium. It failed t o inhibit the growth of Staphylococcus aureus, as did sulfathiazole sodium,
38 1 SUMMARY
A new derivative of sulfanilamide was prepared and tested in vitro against two Gramnegative and two Gram-positive organisms. 1. Trifhydroxymethy1)sulfanilamidomethane is more soluble than sulfanilamide, sulfathiazole, and sulfadiazine i n either alcohol or water. 2. Tri(hydroxymethy1)sulfanilamidomethane was tested in vitro on four microorganisms and found to be less bacteriostatic than sulfathiazole. REFERENCES (1) Adams. R.,Long, P. H., and Johanson. A. J., J . A m . Chcm. Sac., 61,2342(1939). (2) Crossle M. L., Northey, E. H., and Hultquist, M. E.. ibid. 62 532c940). . 3' Gbldfarb. A. R., ibrd. 67, 1352(1945). 4 Crossley. M. L., Noi. ley, E. H., and Hultquist, M. E.,a b d , 60, 2217(1938).
Book Reviews Vitamins and Hormones, Vo.'olume V . Edited by Academic ROBERTS. HARRISand V. THIMANN. Press, Inc., New York, 1947. xvi 478 pp. 15 x 23 cm. Price $7.50. The ever-increasing number of papers on the biological, chemical, and clinical properties of the vitamins and the hormones makes the wisdom of a n annual review in these fields apparent. I n Volume V of these reviews, the authors have been carefully selected, as always, t o condense and evaluate the most important recent developments. The recent synthesis of vitamin A makes a review of such synthetic methods advisable. This is ably done by Nicholas A. Milas of Massachusetts Institute of Technology: The question of the physiological availability of the vitamins is reviewed by Doctors Melnick and Oser. These authors also evaluate the limitations of nonbiological methods for determining the vitamins. Other reviews in the vitamin field deal with thiamine and neurophysiology, the effects of pterylglutamates in man, the vitamin requirements of the chick, and the requirements of the mouse. In the hormone field most of the reviews are of pharmaceutical importance. The reviews consider the properties of the growth and adrenocorticotropic hormones, the effect of exogenous estrogens on the male mammal, the biology of antithyroid agents, the use of androgens in women, and the clinicaluses of testosterone in the male. A valuable feature of the volume is the cumulative index t o the first five volumes.
The Asefitic Treatment of Wounds. By CARLW. WALTER, A.B., M.D., assistant professor of surgery a t Harvard University, and senior asso-
ciate in surgery at the Peter Bent Brigham Hospital in Boston. The Macmillan Company, 372 pp. 21 x 28.5 cm. New York. 1947 ix Price $9.00. Beginning with a history of the development of the concept of asepsis, and discussing in full detail tlie various phases of aseptic technique, this book offers a ready referenee for surgeons, nurses, pharmacists, and bacteriologists, Other hospital personnel concerned with the necessary space and equipment for carrying out aseptic technique in the hospital will also find this volume valuable. A long list of references and a good index add t o its usefulness. Of particular interest t o pharmacists in hospitals is the chapter on the preparation of parenteral fluids in which the author outlines specific procedures for the 'preparation of solutions. The design of the still, source of water supply, equipment necessary, pyrogen test, cleaning of glassware, and sterilization of solutions are all discussed in detail and diagrammatic illustrations of stills, apparatus, etc., are included. Illustrations of the autoclave along with information on its operation are covered in a separate chapter. The importance of aseptic technique and how it is accomplished are emphasized throughout this voluine, giving special attention t o all aspects of the destruction of bacteria in connection with instruments, dressings, and the skin. Routines for carrying out aseptic procedures in the operating room and central supply are outlined in detail along with drawings t o illustrate each step. Air-borne contamination, blood and plasma facilities, control of communicable disease, and maintenance of sterilizing equipment are ,also covered.