WS021 Human papillomavirus DNA in malignant and premalignant skin lesions of renal transplant recipients

WS021 Human papillomavirus DNA in malignant and premalignant skin lesions of renal transplant recipients

Workshops - HPV Infection At the beginning of the XIXth century, EN interested Physicians who were recently clinicians. But they were investigators ...

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Workshops

- HPV Infection

At the beginning of the XIXth century, EN interested Physicians who were recently clinicians. But they were investigators too and the danger was to explain all the facts they observed by the science they have just discovered. We think, now, that the EN is the result of two types of immunologic reaction according to different allergens. Two questions subsist: the predisposition of a person and the pathogenesis of EN. In the near future, we will maybe have the answer with our most recent science: Genetics. WSOI 9 The destiny of the Jewish dermatologists

in Germany in the time of Nationalsocialism S. Eppinger, A. Scholz. Institutfor Dresden,

history

of medicine,

TU

Germany

16% of the physicians in Germany in 1933 were Jews. The share of Jews of the whole population amounted few than I % in this time. Jewish physicians had to suffered under the antijewish laws of the Nationalsocialists. In this connection they were removed from the universities and the public hospitals after 1933. By the law “4. Verordnung zum Reichsbiirgergesetz” they were strucked off the Medical Register on September 30th, 1938. The share of Jews under the dermatologists were amounted about 27%. 566 Jewish dermatologists could be documented. It was possible to detect the destiny of 70% of the Jewish dermatologists. It could be shown, that 16% were murdered in concentration camps, 16% died in Germany in a natural death, 5% survived in Germany, 3% committed suicide and 60% of the Jewish dermatologists emigrated. The USA were th main emigration destination. 40% found a new home there.

HPV Infection of the Skin WSO20 Human papillomavirus cutaneous warts

(HPV) and

C.A. Harwood, J. Breuer ‘, J.M. McGregor, C.M. Proby, I.M. Leigh. Academic Departments of Dermatology; ’ Virology, Royal

Hospitals

NHS

Trust,

London,

UK

Papillomaviruses are small double-stranded DNA viruses showing a high degree of host and epithelial cell specificity. Over 70 HPV types have been described, and these fall into 3 broad categories -cutaneous, mucosal and epidermodysplasia verruciformis (EV)-related. The diversity of the clinicopathological manifestations of benign cutaneous HPV infection is considerable. For the majority of immunocompetent individuals viral warts are usually little more than a minor inconvenience as spontaneous resolution tends to occur. However, for those with abnormalities of cell mediated immunity, HPV infection may be extensive, persistent and refractory to treatment. EV is perhaps the most distinctive example of this. Various primary immunodeficiency states may also be accompanied by extensive viral warts, as may certain secondary immunodeficiency disorders including haematological malignancies, HIV infection and iatrogenic immunosuppression. Data concerning the spectrum of HPV types harboured by benign cutaneous lesions are relatively

S83

of the Skin

limited. However, evidence is emerging to suggest that the types detected in lesions from immunosuppressed individuals may be rather more diverse than those found in the immunocompetent population. WS021

Human papillomavirus DNA in malignant and premalignant skin lesions of renal transplant recipients

L.M. de Jong-Tieben ’ , J.N. Bouwes Bavinck ’ , R.J.M. Berkhout’, J. ter Schegget2. ‘Dep. Dermatology, University Hospital Center, Amsterdam,

Leiden; ‘Dep. The Netherlands

Virology,

Acad.

Medical

Comparing the data of different studies on human papillomavirus (HPV) infection in skin cancers from renal transplant recipients, it is obvious that no firm statements can be formulated about the true prevalence of HPV DNA in such cancers and about the HPV types that are involved. This controversy is probably largely attributable to differences in the techniques used. We designed a two step (nested) consensus polymerase chain reaction (PCR) method, especially suitable for the detection of epidermodysplasia verruciformis (EV)-related HPVs at high sensitivity. With this technique we analysed a series of biopsies from skin lesions from renal transplant recipients and found HPV DNA in 49 (80%) of 61 squamous cell carcinomas, in 4 (50%) of 8 basal cell carcinomas, in 14 (93%) of 15 actinic keratoses, in 2 (40%) of 5 cases of Bowen’s disease and in 4 (57%) of 7 kerathoacanthomas. HPV DNA typing revealed a wide spectrum of EV-associated HPV types, including 6 putative new HPV types. In more than 30% of the lesions mixed HPV infections were found. Furthermore the same HPV types were often found in different skin lesions from the same patient.

WS022 UV and ~53 mutations in post-transplant skin cancer J.M. McGregor. Dept Photobiology,

St. John’s Institute of Dermatology and Imperial Cancer Skin Tumour Laboratory, Royal London Hospital, London, UK

Renal transplant recipients are at considerably higher risk of developing non-melanoma skin cancers (NMSC) than the general population. Human papillomaviruses (HPV), immunosuppressive drugs and solar ultraviolet radiation (UVR) have each been implicated. A predominant role for UVR is suggested by several lines of investigation. Firstly, the cumulative incidence of skin cancers in transplant patients resident in Australia is significantly higher than those living in temperate North European climates. Secondly, skin cancers, particularly squamous cell carcinomas, occur almost exclusively on chronically sun-exposed skin. Finally, sequence analysis of the p53 tumour suppressor gene reveals that the majority of skin cancers from renal transplant patients contain UV-specific mutations. The high prevalence of p53 mutation suggests that any functional role for HPV in skin cancer is unlikely to involve ubiquitin-mediated p53 degradation.