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10C. Reproductive biology: Steroids on reproductive organs 347. Endogenous concentration of gestagens and their effect on endometrial histology
319
Abstracts comparative pathology data and permit a comparison of mammary lesions seen in animals with those cases of human breast neoplasia and concurrent analysis of animal species as predictive models in human breast cancer research. Further, such studies might result in the standardization of nomentaclureand classification of canine and simian mammary neoplasms and nonmalignant proliferative breast lesions.
1OC. Reproductive biology: Steroids on reproductive organs 347. Endogenous concentration of gestagens and their effect on endometrial histology
ESCALANTE-LEGARRETA, M., GONZALEZ-DIDDI,M. and CORTES-GALLEGOS, V., Hospital de GinecoObstetricia (G.C.) JSSSTE and Departamento de Jnvestigacion Cientifica, C.M.N., J.M.S.S., Mexico 73-032, Mexico, D.F. Plasma progesterone (P) 17a-hydroxyprogesterone (17 OHP) and 20cc-dihydroprogesterone (20 OH-P) were quantifified by radioimmunoassay during the critical periovulatory period (CPP) in the human menstrual cycle; simultaneously, endometrial biopsies were taken in 60 normal menstruating women. Levels of 20 OH-P never exceeded 0,039 ng/ml in the CPP (pre-ovulatory range : 0.0040.072, R = 0,039 + 0.014fS.D.)nost-ovulatorvrange = 0~0160~109, x = 0.060 i 0.019, N’- 109). In 7 studies-17 OH-P values in the CPP overlapped considerably those of P and no vacuolization or secretory endometrial changes were found. In another 9 instances P values in the CPP ranged from 1.7 to 4.2 ng/ml higher than 17 OH-P values (0.8 to 1.9 ng/ml) and yet no endometrial secretory changes occurred. However, P values over 5,7ng/ml always showed definite secretory changes (N = 22). Our results suggest: (a) 20 OH-P does not play a roleinsecretorychanges,(b)previousreportsofhigh17 OH-P pre-ovulatory values do not necessarily mean secretory changes and (c) P values from 2.7 to 3.6 ng/ml showed definite thermogenic effects on basal body temperature but no evidence of progestational activity.
34% The effect of Norethynodrel on the reproductive system of men’ with idiopathic gynecomastia
CHEMODANOV, V. I. and SELVEROVA, N. B., Laboratory of Organ Transplantation and Department of Operative Surgery, Lumumba’s University; Institute of the Experimental Endocrinology and Biochemistry of Hormones, Moscow, U.S.S.R. The preparation was administered orally (100 mg/day) with the purpose of obtaining a therapeutic effect and to study the excretion of steroids and gonadotropins as well as the germinal function of the testis. Amoung half the patients, clinical remission was observed on the 30th day. The urinary excretion of the 17-KS and its fractions were subnormal. The decrease of 17-OHCS was noted, whereas an increase in THF/THE was found and there was not any change in the amount of F, E, S. The estrogen excretion fell to the normal value. The mobility of the spermatozoa, the concentration of fructose and acid phosphatase activity decreased in the semen of the treated patients. The results obtained show a positive effect of norethynodrel in gynecomastia. except on the germinal function of the testis.
349. Progesterone and reduced reproduction ia heat-acclimatized female rats
BEDRAK,E. and SOD-MORIAH,U. A., Department of Biology, University of the Negev, Beer Sheva, Israel Acclimatization of adult female rats (HA) to a high environmental temperature (35 “C) adversely reproductive performance possibly related to increased progesterone levels as compared with controls [Cl (22°C). HA females show a prolonged estrus cycle, pseudopregnancy and pregnancy and the process of parturition is irregular. In addition, pregnancy is successfully maintained to midterm with fewer corpora luteu [CL] than are required by C animals. Progesterone was determined in peripheral blood plasma and CL using the competitive binding procedure. It was determined in cycling ammals and in those in the first 4 days of pregnancy. Progesterone concentrations are 20% higher in C animals than in HA (P < 0.01) at proestrus whereas at diestrus HA values are 44% higher (P < 0.05) than in C. In early pregnancy, plasma progesterone levels of HA animals are constantly higher than in C by 20, 16, 5 1 and 20% for days 1, 2, 3 and 4, respectively. There is no significant difference in mean CL weight between C and HA animals. Progesterone content of CL of day 4 of pregnancy is higher in HA than in C animals by 262”;. These data confirm the hypothesis of increased progesterone levels in HA animals. This could be due to enhanced synthesis, or slower catabolism of progesterone, or both. 350. Dynamics
of testosterone concentrations in sheep
and eestradiol-17jl
plasma
TERQUI, M., GARNIER, D. H., COTTA, Y. and ROUGER, Y. A., Station de Physiologie Animale C.R.V.Z. 37380 Nouzilly, France We have observed large and rapid unexplained variations in peripheral blood plasma concentration of testosterone in ramsand 17goestradiol in ewes. To evaluate these variations we have performed two studies. In the first we have simulated pulse secretions by the gonads using testicular vein injection of 3H-testosterone and ovarian vein injection of 17-p-3H-oestradiol. Jugular blood samples were taken every 1Osec from 5 min pre- to 10min post-injection and at 5min intervals the following h. In the ram unconjugated testosterone radioactivity was maximal at 50 set (this represents 95% of total plasma radioactivity) decreases rapidly to half maximum radioactivity at 150sec and to 10% of maximum at 20 min. In the ewe unconjugated 178oestradiol radioactivity increased within 20 sec. was maximum at 50 set (95% of total plasma radioactivity), and by 90 set was one-half maximum. In the second study we have measured endogenous testosterone secretion by rapid serial sampling (10 set) of the testicular vein, We observed large variations in concentration (IO-22 ng/ml). The very rapid disappearance of these steroids from plasma and endogenous pulse secretion, as observed for testosterone, may explain the variations in peripheral blood concentration observed for these two steroids. 351. Effect of different levels of circulating testosterone on plasma LH and response of plasma testosterone to HCG in the prepuberal male miniature pig
ELSAESSER,F., ELLENM)RFF,F., PARVIZI, N. and KOENIG,A., Division of Clinical and Experimental Endocrinology University Womens’ Hospital and Division of Reproductive Biology, Department of Animal Science, Gottingen, FRG. Previously we have shown that in the prepuberal
male
Abstracts comparative pathology data and permit a comparison of mammary lesions seen in animals with those cases of human breast neoplasia and concurrent analysis of animal species as predictive models in human breast cancer research. Further, such studies might result in the standardization of nomentaclureand classification of canine and simian mammary neoplasms and nonmalignant proliferative breast lesions.
1OC. Reproductive biology: Steroids on reproductive organs 347. Endogenous concentration of gestagens and their effect on endometrial histology
ESCALANTE-LEGARRETA, M., GONZALEZ-DIDDI,M. and CORTES-GALLEGOS, V., Hospital de GinecoObstetricia (G.C.) JSSSTE and Departamento de Jnvestigacion Cientifica, C.M.N., J.M.S.S., Mexico 73-032, Mexico, D.F. Plasma progesterone (P) 17a-hydroxyprogesterone (17 OHP) and 20cc-dihydroprogesterone (20 OH-P) were quantifified by radioimmunoassay during the critical periovulatory period (CPP) in the human menstrual cycle; simultaneously, endometrial biopsies were taken in 60 normal menstruating women. Levels of 20 OH-P never exceeded 0,039 ng/ml in the CPP (pre-ovulatory range : 0.0040.072, R = 0,039 + 0.014fS.D.)nost-ovulatorvrange = 0~0160~109, x = 0.060 i 0.019, N’- 109). In 7 studies-17 OH-P values in the CPP overlapped considerably those of P and no vacuolization or secretory endometrial changes were found. In another 9 instances P values in the CPP ranged from 1.7 to 4.2 ng/ml higher than 17 OH-P values (0.8 to 1.9 ng/ml) and yet no endometrial secretory changes occurred. However, P values over 5,7ng/ml always showed definite secretory changes (N = 22). Our results suggest: (a) 20 OH-P does not play a roleinsecretorychanges,(b)previousreportsofhigh17 OH-P pre-ovulatory values do not necessarily mean secretory changes and (c) P values from 2.7 to 3.6 ng/ml showed definite thermogenic effects on basal body temperature but no evidence of progestational activity.
34% The effect of Norethynodrel on the reproductive system of men’ with idiopathic gynecomastia
CHEMODANOV, V. I. and SELVEROVA, N. B., Laboratory of Organ Transplantation and Department of Operative Surgery, Lumumba’s University; Institute of the Experimental Endocrinology and Biochemistry of Hormones, Moscow, U.S.S.R. The preparation was administered orally (100 mg/day) with the purpose of obtaining a therapeutic effect and to study the excretion of steroids and gonadotropins as well as the germinal function of the testis. Amoung half the patients, clinical remission was observed on the 30th day. The urinary excretion of the 17-KS and its fractions were subnormal. The decrease of 17-OHCS was noted, whereas an increase in THF/THE was found and there was not any change in the amount of F, E, S. The estrogen excretion fell to the normal value. The mobility of the spermatozoa, the concentration of fructose and acid phosphatase activity decreased in the semen of the treated patients. The results obtained show a positive effect of norethynodrel in gynecomastia. except on the germinal function of the testis.
349. Progesterone and reduced reproduction ia heat-acclimatized female rats
BEDRAK,E. and SOD-MORIAH,U. A., Department of Biology, University of the Negev, Beer Sheva, Israel Acclimatization of adult female rats (HA) to a high environmental temperature (35 “C) adversely reproductive performance possibly related to increased progesterone levels as compared with controls [Cl (22°C). HA females show a prolonged estrus cycle, pseudopregnancy and pregnancy and the process of parturition is irregular. In addition, pregnancy is successfully maintained to midterm with fewer corpora luteu [CL] than are required by C animals. Progesterone was determined in peripheral blood plasma and CL using the competitive binding procedure. It was determined in cycling ammals and in those in the first 4 days of pregnancy. Progesterone concentrations are 20% higher in C animals than in HA (P < 0.01) at proestrus whereas at diestrus HA values are 44% higher (P < 0.05) than in C. In early pregnancy, plasma progesterone levels of HA animals are constantly higher than in C by 20, 16, 5 1 and 20% for days 1, 2, 3 and 4, respectively. There is no significant difference in mean CL weight between C and HA animals. Progesterone content of CL of day 4 of pregnancy is higher in HA than in C animals by 262”;. These data confirm the hypothesis of increased progesterone levels in HA animals. This could be due to enhanced synthesis, or slower catabolism of progesterone, or both. 350. Dynamics
of testosterone concentrations in sheep
and eestradiol-17jl
plasma
TERQUI, M., GARNIER, D. H., COTTA, Y. and ROUGER, Y. A., Station de Physiologie Animale C.R.V.Z. 37380 Nouzilly, France We have observed large and rapid unexplained variations in peripheral blood plasma concentration of testosterone in ramsand 17goestradiol in ewes. To evaluate these variations we have performed two studies. In the first we have simulated pulse secretions by the gonads using testicular vein injection of 3H-testosterone and ovarian vein injection of 17-p-3H-oestradiol. Jugular blood samples were taken every 1Osec from 5 min pre- to 10min post-injection and at 5min intervals the following h. In the ram unconjugated testosterone radioactivity was maximal at 50 set (this represents 95% of total plasma radioactivity) decreases rapidly to half maximum radioactivity at 150sec and to 10% of maximum at 20 min. In the ewe unconjugated 178oestradiol radioactivity increased within 20 sec. was maximum at 50 set (95% of total plasma radioactivity), and by 90 set was one-half maximum. In the second study we have measured endogenous testosterone secretion by rapid serial sampling (10 set) of the testicular vein, We observed large variations in concentration (IO-22 ng/ml). The very rapid disappearance of these steroids from plasma and endogenous pulse secretion, as observed for testosterone, may explain the variations in peripheral blood concentration observed for these two steroids. 351. Effect of different levels of circulating testosterone on plasma LH and response of plasma testosterone to HCG in the prepuberal male miniature pig
ELSAESSER,F., ELLENM)RFF,F., PARVIZI, N. and KOENIG,A., Division of Clinical and Experimental Endocrinology University Womens’ Hospital and Division of Reproductive Biology, Department of Animal Science, Gottingen, FRG. Previously we have shown that in the prepuberal
male