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111 CORRELATION BETWEEN HORMONAL STATUS WITH BONE DENSITOMETRY AND BONE TURNOVER IN POSTMENOPAUSAL WOMEN
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9th European Congress on Menopause and Andropause / Maturitas 71, Supplement 1 (2012) S1–S82
110 EXPRESSION OF LEPTIN RECEPTORS ON OSTEOBLASTS IN OVARIECTOMIZED RATS WITH DIFFERENT NUTRITIONAL DIETS L. Xu 1 , L. Qiao 1 , D. Yang 2 , L. Deng 3 . 1 Obstetrics & Gynecology, West China Second University Hospital, Sichuan University; 2 Department of Radiology, West China Fourth Hospital of Sichuan University; 3 Stem Cells and Tissue Engineering, West China Hospital of Sichuan University, Chengdu, China Objectives: To investigate the molecular mechanisms of leptin on bone, the functions of different leptin receptors (OB-Ra and OB-Rb) on the membrane of osteoblasts and their signal transduction pathways. Material and methods: Wistar rats were randomly divided into four groups, sham operation with ordinary diet group (n=20), sham operation with high nutrition group (n=20), ovariectomy with ordinary diet group (n=20), and ovariectomy with high nutrition group (n=20). mRNA expressions of OB-Ra and OB-Rb were measured. Results: PCR electrophoresis results showed lost expression of OB-Rb in two high-nutrition groups. In the sham operation with high nutrition group, the loss of OB-Rb expression accounts for 20%. In the ovariectomy with high nutrition group, this number grows to 60%. In sham operation with high nutrition group, ovariectomy with normal diet group and ovariectomy with high-nutrition group, the lost expression of short receptor OB-Ra accounts for 40%, 20%, and 20% respectively. By semi-quantitative analyzing the electrophoresis on mRNA expressions did not show significant differences between different groups. Conclusions: The physiological functions of leptin on bone may be executed through regulating receptors express differentially. Obesity and nutritional factors might influence expression of the same receptors.
111 CORRELATION BETWEEN HORMONAL STATUS WITH BONE DENSITOMETRY AND BONE TURNOVER IN POSTMENOPAUSAL WOMEN S. Ziaei, Z. Haidari. Tarbiat Modares University, Tehran, Iran Introduction: To determine the correlation between hormonal status with bone densitometry and bone turnover in postmenopausal women. Methods: 150 non-surgical postmenopausal women were enrolled to this cross-sectional study. Participants completed a questionnaire and underwent a medical exam, and serum alkaline phosphates (ALP) and urine Ca/Cr ratio measured as bone turnover markers. Also, Bone densitometry (DAX) of the lumbar spine and the proximal femoral neck measured. Serum sex hormone binding globulin (SHBG), estradiol and testosterone levels were determined simultaneously. Then, the correlation between hormonal status with bone densitometry and bone turnover were evaluated. Results: There were significant correlations between serum testosterone level with urine Ca/Cr, lumbar spine and femoral bone densitometry (P:< 0.0001, r: 0.61, r: −0.61, r: −0.34, respectively). Also, there was negative correlation between serum estradiol level and serum alkaline phosphates (p: 0.006, r: −0.22. Conclusion: Estradiol and testosterone correlate with bone turnover and bone densitometry.
Ovarian Cancer
112 OVARIAN TUMORS AND THE MANNER OF THEIR SURGICAL TRETMENT IN GOC KRAGUJEVAC, SERBIA T. Kastratovic 1 , A. Zivanovic 2 , Z. Protrka 2 , P. Arsenijevic 2 . 1 Department of Obstetrics and Gynaecology; 2 Clinical Center Kragujevac, Kragujevac, Serbia Ovarian tumors are histogenetic and histologically very heterogeneous group of tumors and can be treated conservatively and/or surgically. The extent of surgical intervention depends on clinical, pathohistological and intraoperative findings, age of patients and her desire to give birth. In our retrospective study, we observed the patients with diagnosed ovarian tumor, who were surgically treated in GOC Kragujevac, Serbia in the period
from 01.01.2009. to 31.12.2010. In 152 women we diagnosed the ovarian tumor and in 87% of this cases tumor was benign and in 13% it was of malignant nature. In the patients with ovarian tumor pathohistological findings confirmed that the most common were the tumors of epithelial cells (43.59%) and 10.82% of these tumors had malignant character (cystadenocarcinoma serosum papillare ovarii 6.32% and cystadenocarcinoma mucinosum ovarii 4.5%). Tumor mass in most cases was removed with less radical surgical interventions (adnesectomia, cystectomia and resection of the ovaries) 59.89%, and 49.58% of them laparoscopically. The high percentage of radical surgery, 40.11% is mainly a consequence of associated pathological conditions (25.97%), mostly fibroids of the uterus.
113 BONE MASS IN WOMEN WITH THE OVARIAN CANCER M. Milivojevic. Institute of Medical Biochemistry, Clinical Centre of Serbia, Belgrade, Serbia Objective: Ovarian cancer ranks as the fifth leading cause of death from cancer and requires invasive treatment surgery and often chemotherapy, which both can reduce the bone mass. The aim of our study was to evaluate the bone mineral density (BMD) in patients with ovarian cancer before treatment. Method: A total of 36 patients with benign ovarian tumors, and 44 patients with epithelial ovarian cancer, without bone metastases, were examined. All of them were in perimenopause or menopause period. Bone density of lumbar spine and femoral neck was measured by dual energy x-ray absorptiometry (DEXA). Age, height, body weight, body mass index (BMI) and BMD of spine and femur were evaluated between these group and correlation with cancer stage was studied. Results: There was no significant difference of age (p=0066), body weight (p=0.140), and BMI (p=0.06) between groups. Femoral and spinal BMD measurements also failed to exhibit any significant difference between groups (p=0.489 i p=0.997). Moreover, BMD measurements in patients with their early and advanced OC stages were not significantly different: femoral and spinal BMD ratios were p=0.887 and p=0.489, respectively. Conclusion: BMD is not affected by OC but our recommendation is to perform study with larger sample of patients.
Preventive Medicine
114 YUMEIHO THERAPY AND MENOPAUSE Z. Mehraban 1 , L. Alizadeh 2 . 1 Department of Medicine; 2 Ardabil Branch, Islamic Azad University, Ardabil, Iran Yumeiho therapy is one of the massage therapies to treat many diseases of body and mind are used. This medicine is based on harmony, balance and symmetry in the body. In this treatment, therapist improves the lack of any symmetry and disharmony in the body and internal organs functioning. The main aim of the Yumeiho therapy is to correct the pelvic bones position and to set the vertebral column properly, and thus to mobilize the autotherapeutic forces of the organism and to reach the cause of pathological state. “Yumeiho therapy” can use to treat women’s problems such as menstrual disorders, menstrual pain, menstruation stops and partly prevent menopause, osteoporoses, pregnancy-related nausea, dysmenorrhea, prolapsed uterus and . . . Yumeiho Therapists can postpone menopausal age by treatment techniques and balancing the women’s hormone and. Since in modern societies the age of menopause decreased because of cultural condition and incorrect diet, even in cases get less than 40 years, application of this method can be very effective. Therapists can prevent premature menopause by correcting diet and offering techniques that the patient can repeat some of them at home. Also it may help diminish symptoms and reduce the risk of serious complications (such as osteoporosis and cardiovascular disease) associated with menopause.
9th European Congress on Menopause and Andropause / Maturitas 71, Supplement 1 (2012) S1–S82
110 EXPRESSION OF LEPTIN RECEPTORS ON OSTEOBLASTS IN OVARIECTOMIZED RATS WITH DIFFERENT NUTRITIONAL DIETS L. Xu 1 , L. Qiao 1 , D. Yang 2 , L. Deng 3 . 1 Obstetrics & Gynecology, West China Second University Hospital, Sichuan University; 2 Department of Radiology, West China Fourth Hospital of Sichuan University; 3 Stem Cells and Tissue Engineering, West China Hospital of Sichuan University, Chengdu, China Objectives: To investigate the molecular mechanisms of leptin on bone, the functions of different leptin receptors (OB-Ra and OB-Rb) on the membrane of osteoblasts and their signal transduction pathways. Material and methods: Wistar rats were randomly divided into four groups, sham operation with ordinary diet group (n=20), sham operation with high nutrition group (n=20), ovariectomy with ordinary diet group (n=20), and ovariectomy with high nutrition group (n=20). mRNA expressions of OB-Ra and OB-Rb were measured. Results: PCR electrophoresis results showed lost expression of OB-Rb in two high-nutrition groups. In the sham operation with high nutrition group, the loss of OB-Rb expression accounts for 20%. In the ovariectomy with high nutrition group, this number grows to 60%. In sham operation with high nutrition group, ovariectomy with normal diet group and ovariectomy with high-nutrition group, the lost expression of short receptor OB-Ra accounts for 40%, 20%, and 20% respectively. By semi-quantitative analyzing the electrophoresis on mRNA expressions did not show significant differences between different groups. Conclusions: The physiological functions of leptin on bone may be executed through regulating receptors express differentially. Obesity and nutritional factors might influence expression of the same receptors.
111 CORRELATION BETWEEN HORMONAL STATUS WITH BONE DENSITOMETRY AND BONE TURNOVER IN POSTMENOPAUSAL WOMEN S. Ziaei, Z. Haidari. Tarbiat Modares University, Tehran, Iran Introduction: To determine the correlation between hormonal status with bone densitometry and bone turnover in postmenopausal women. Methods: 150 non-surgical postmenopausal women were enrolled to this cross-sectional study. Participants completed a questionnaire and underwent a medical exam, and serum alkaline phosphates (ALP) and urine Ca/Cr ratio measured as bone turnover markers. Also, Bone densitometry (DAX) of the lumbar spine and the proximal femoral neck measured. Serum sex hormone binding globulin (SHBG), estradiol and testosterone levels were determined simultaneously. Then, the correlation between hormonal status with bone densitometry and bone turnover were evaluated. Results: There were significant correlations between serum testosterone level with urine Ca/Cr, lumbar spine and femoral bone densitometry (P:< 0.0001, r: 0.61, r: −0.61, r: −0.34, respectively). Also, there was negative correlation between serum estradiol level and serum alkaline phosphates (p: 0.006, r: −0.22. Conclusion: Estradiol and testosterone correlate with bone turnover and bone densitometry.
Ovarian Cancer
112 OVARIAN TUMORS AND THE MANNER OF THEIR SURGICAL TRETMENT IN GOC KRAGUJEVAC, SERBIA T. Kastratovic 1 , A. Zivanovic 2 , Z. Protrka 2 , P. Arsenijevic 2 . 1 Department of Obstetrics and Gynaecology; 2 Clinical Center Kragujevac, Kragujevac, Serbia Ovarian tumors are histogenetic and histologically very heterogeneous group of tumors and can be treated conservatively and/or surgically. The extent of surgical intervention depends on clinical, pathohistological and intraoperative findings, age of patients and her desire to give birth. In our retrospective study, we observed the patients with diagnosed ovarian tumor, who were surgically treated in GOC Kragujevac, Serbia in the period
from 01.01.2009. to 31.12.2010. In 152 women we diagnosed the ovarian tumor and in 87% of this cases tumor was benign and in 13% it was of malignant nature. In the patients with ovarian tumor pathohistological findings confirmed that the most common were the tumors of epithelial cells (43.59%) and 10.82% of these tumors had malignant character (cystadenocarcinoma serosum papillare ovarii 6.32% and cystadenocarcinoma mucinosum ovarii 4.5%). Tumor mass in most cases was removed with less radical surgical interventions (adnesectomia, cystectomia and resection of the ovaries) 59.89%, and 49.58% of them laparoscopically. The high percentage of radical surgery, 40.11% is mainly a consequence of associated pathological conditions (25.97%), mostly fibroids of the uterus.
113 BONE MASS IN WOMEN WITH THE OVARIAN CANCER M. Milivojevic. Institute of Medical Biochemistry, Clinical Centre of Serbia, Belgrade, Serbia Objective: Ovarian cancer ranks as the fifth leading cause of death from cancer and requires invasive treatment surgery and often chemotherapy, which both can reduce the bone mass. The aim of our study was to evaluate the bone mineral density (BMD) in patients with ovarian cancer before treatment. Method: A total of 36 patients with benign ovarian tumors, and 44 patients with epithelial ovarian cancer, without bone metastases, were examined. All of them were in perimenopause or menopause period. Bone density of lumbar spine and femoral neck was measured by dual energy x-ray absorptiometry (DEXA). Age, height, body weight, body mass index (BMI) and BMD of spine and femur were evaluated between these group and correlation with cancer stage was studied. Results: There was no significant difference of age (p=0066), body weight (p=0.140), and BMI (p=0.06) between groups. Femoral and spinal BMD measurements also failed to exhibit any significant difference between groups (p=0.489 i p=0.997). Moreover, BMD measurements in patients with their early and advanced OC stages were not significantly different: femoral and spinal BMD ratios were p=0.887 and p=0.489, respectively. Conclusion: BMD is not affected by OC but our recommendation is to perform study with larger sample of patients.
Preventive Medicine
114 YUMEIHO THERAPY AND MENOPAUSE Z. Mehraban 1 , L. Alizadeh 2 . 1 Department of Medicine; 2 Ardabil Branch, Islamic Azad University, Ardabil, Iran Yumeiho therapy is one of the massage therapies to treat many diseases of body and mind are used. This medicine is based on harmony, balance and symmetry in the body. In this treatment, therapist improves the lack of any symmetry and disharmony in the body and internal organs functioning. The main aim of the Yumeiho therapy is to correct the pelvic bones position and to set the vertebral column properly, and thus to mobilize the autotherapeutic forces of the organism and to reach the cause of pathological state. “Yumeiho therapy” can use to treat women’s problems such as menstrual disorders, menstrual pain, menstruation stops and partly prevent menopause, osteoporoses, pregnancy-related nausea, dysmenorrhea, prolapsed uterus and . . . Yumeiho Therapists can postpone menopausal age by treatment techniques and balancing the women’s hormone and. Since in modern societies the age of menopause decreased because of cultural condition and incorrect diet, even in cases get less than 40 years, application of this method can be very effective. Therapists can prevent premature menopause by correcting diet and offering techniques that the patient can repeat some of them at home. Also it may help diminish symptoms and reduce the risk of serious complications (such as osteoporosis and cardiovascular disease) associated with menopause.