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[120-POS]
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Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156
[119-POS] ADMA is lower and endothelial-dependent vascular function is improved in hypercholesterolemia with acute caffeine treatment Robert Powers a, Paul Kukunas a, Marcia Gallaher b (a University of Pittsburgh, Pittsburgh, PA, USA, b MageeWomens Research Institute, Pittsburgh, PA, USA) Objectives: Coffee consumption is associated with a significant decreased risk of CVD and a decreased risk of preeclampsia, however the mechanism behind this relationship is unknown. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthase and metabolized primarily by the enzyme DDAH1. ADMA is an independent risk factor of CVD among low-, mediumand high-risk patients, and significantly elevated in hypercholesterolemia and preeclampsia. The focus of this study was to investigate if caffeine treatment affects circulating levels of ADMA and vascular function in the hypercholesterolemia apo E / mouse, which is known to have elevated ADMA. Methods: Female apo E / mice were treated with caffeine (10 lg/day) via drinking water for 7 days. Endothelial-dependent and independent-vascular function from caffeine treated (n = 5) and control (n = 5) mice were investigated. Plasma ADMA, SDMA, arginine, cholesterol, triglycerides and blood pressure were measured. Results: ADMA was significantly lower in caffeine treated mice (0.09 ± 0.04 vs. 0.24 ± 0.05lmol/L, p < 0.01). There was no difference in blood pressure prior to caffeine treatment between groups, but was significantly lower in caffeine treated mice (119 ± 4/88 ± 5) compared to controls (134 ± 5/104 ± 5 mmHg, p < 0.05). Methacholine-induced endothelial-dependent relaxation was significantly improved in arteries from caffeine treated mice (65 ± 5%) compared to control mice (49 ± 5%, p < 0.05). Conclusions: Acute caffeine treatment in female hypercholesterolemic apo E / mice is associated with lower ADMA, a decrease in blood pressure and a significant improvement in endothelial-dependent relaxation in the absence of changes in circulating cholesterol. The potential beneficial effects of coffee consumption on cardiovascular risk and preeclampsia may be related to changes in the metabolism of ADMA. Disclosures: R. Powers: None. P. Kukunas: None. M. Gallaher: None. doi:10.1016/j.preghy.2014.10.125
[120-POS] Association between cystatin C and markers of renal function in preeclamptic pregnant women Roberto A. Costa a, Maria T. Peracoli b, Mariana Romão b, Ingrid C. Weel b, Joelcio F. Abbade a, Jose C. Peracoli a (a Botucatu Medical School, Sao Paulo State University, Botucatu, Brazil, b Institute of Biosciences, Sao Paulo State University, Botucatu, Brazil)
Objectives: Preeclampsia is a complex multi-systemic disorder of human pregnancy characterized by hypertension and proteinuria, and frequently associated with altered renal function. Serum level of cystatin C seems to reflect the glomerular filtration rate and is associated with preeclampsia. Considering that the serum level of cystatin C is a good marker for glomerular filtration the present study aimed to compare the levels of cystatin C, urea and creatinine in serum and proteinuria in preeclamptic pregnant women. Methods: Sixty-seven women with preeclampsia and 20 normotensive pregnant women matched for gestational age were included. Preeclamptic women were classified according to the proteinuric levels in 24 h-urine as: mild proteinuria (0.3–1.99 g/24 h; n = 27); severe proteinuria (2.0–9.99 g/24 h; n = 28) or massive proteinuria (P10.0 g/ 24 h; n = 12). Serum levels of urea and creatinine were determined using commercially available assay kits, and cystatin C levels were evaluated using an enzyme-linked immunosorbent assay. Comparisons between pregnant groups were analyzed by non-parametric tests with significance set at p < 0.05. Results: Cystatin C level in preeclamptic women with massive proteinuria was significantly higher than in severe and mild proteinuria groups as well as in the normotensive one. Moreover, patients with severe proteinuria had higher levels of cystatin C compared with mild proteinuria. The levels of urea and creatinine were higher in massive proteinuria compared with mild proteinuria. Correlation analysis between cystatin C and the other parameters showed association with proteinuria (r = 0.3262; p = 0.0043), urea (r = 0.3493; p = 0.0023) and creatinine (r = 0.3319; p = 0.0037). Conclusions: Association between serum level of cystatin C with the markers of renal function such as urea and creatinine, as well as with different degrees of proteinuria, suggest that cystatin C may be employed as a marker of renal injury and preeclampsia severity. Disclosures: R.A. Costa: None. M.T. Peracoli: None. M. Romão: None. I.C. Weel: None. J.F. Abbade: None. J.C. Peracoli: None. doi:10.1016/j.preghy.2014.10.126
[121-POS] Maternal blood pressure as a risk factor of hypertension and hyperlipidemia in later life Kanji Tanaka, Maika Oishi, Hitomi Chiba, Kaori Iino, Hideki MIzunuma (Hirosaki University Hospital, Hirosaki, Japan) Objectives: The objective of this study was to determine whether the blood pressure level during pregnancy is a risk factor of cardiovascular disease in later life. Methods: We used the Maternity Health Record Books, provided for all pregnant women by the Japanese government since 1942, as a source of past pregnancy records. Of 641 identified subjects, 584 participants were included
Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156
[119-POS] ADMA is lower and endothelial-dependent vascular function is improved in hypercholesterolemia with acute caffeine treatment Robert Powers a, Paul Kukunas a, Marcia Gallaher b (a University of Pittsburgh, Pittsburgh, PA, USA, b MageeWomens Research Institute, Pittsburgh, PA, USA) Objectives: Coffee consumption is associated with a significant decreased risk of CVD and a decreased risk of preeclampsia, however the mechanism behind this relationship is unknown. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthase and metabolized primarily by the enzyme DDAH1. ADMA is an independent risk factor of CVD among low-, mediumand high-risk patients, and significantly elevated in hypercholesterolemia and preeclampsia. The focus of this study was to investigate if caffeine treatment affects circulating levels of ADMA and vascular function in the hypercholesterolemia apo E / mouse, which is known to have elevated ADMA. Methods: Female apo E / mice were treated with caffeine (10 lg/day) via drinking water for 7 days. Endothelial-dependent and independent-vascular function from caffeine treated (n = 5) and control (n = 5) mice were investigated. Plasma ADMA, SDMA, arginine, cholesterol, triglycerides and blood pressure were measured. Results: ADMA was significantly lower in caffeine treated mice (0.09 ± 0.04 vs. 0.24 ± 0.05lmol/L, p < 0.01). There was no difference in blood pressure prior to caffeine treatment between groups, but was significantly lower in caffeine treated mice (119 ± 4/88 ± 5) compared to controls (134 ± 5/104 ± 5 mmHg, p < 0.05). Methacholine-induced endothelial-dependent relaxation was significantly improved in arteries from caffeine treated mice (65 ± 5%) compared to control mice (49 ± 5%, p < 0.05). Conclusions: Acute caffeine treatment in female hypercholesterolemic apo E / mice is associated with lower ADMA, a decrease in blood pressure and a significant improvement in endothelial-dependent relaxation in the absence of changes in circulating cholesterol. The potential beneficial effects of coffee consumption on cardiovascular risk and preeclampsia may be related to changes in the metabolism of ADMA. Disclosures: R. Powers: None. P. Kukunas: None. M. Gallaher: None. doi:10.1016/j.preghy.2014.10.125
[120-POS] Association between cystatin C and markers of renal function in preeclamptic pregnant women Roberto A. Costa a, Maria T. Peracoli b, Mariana Romão b, Ingrid C. Weel b, Joelcio F. Abbade a, Jose C. Peracoli a (a Botucatu Medical School, Sao Paulo State University, Botucatu, Brazil, b Institute of Biosciences, Sao Paulo State University, Botucatu, Brazil)
Objectives: Preeclampsia is a complex multi-systemic disorder of human pregnancy characterized by hypertension and proteinuria, and frequently associated with altered renal function. Serum level of cystatin C seems to reflect the glomerular filtration rate and is associated with preeclampsia. Considering that the serum level of cystatin C is a good marker for glomerular filtration the present study aimed to compare the levels of cystatin C, urea and creatinine in serum and proteinuria in preeclamptic pregnant women. Methods: Sixty-seven women with preeclampsia and 20 normotensive pregnant women matched for gestational age were included. Preeclamptic women were classified according to the proteinuric levels in 24 h-urine as: mild proteinuria (0.3–1.99 g/24 h; n = 27); severe proteinuria (2.0–9.99 g/24 h; n = 28) or massive proteinuria (P10.0 g/ 24 h; n = 12). Serum levels of urea and creatinine were determined using commercially available assay kits, and cystatin C levels were evaluated using an enzyme-linked immunosorbent assay. Comparisons between pregnant groups were analyzed by non-parametric tests with significance set at p < 0.05. Results: Cystatin C level in preeclamptic women with massive proteinuria was significantly higher than in severe and mild proteinuria groups as well as in the normotensive one. Moreover, patients with severe proteinuria had higher levels of cystatin C compared with mild proteinuria. The levels of urea and creatinine were higher in massive proteinuria compared with mild proteinuria. Correlation analysis between cystatin C and the other parameters showed association with proteinuria (r = 0.3262; p = 0.0043), urea (r = 0.3493; p = 0.0023) and creatinine (r = 0.3319; p = 0.0037). Conclusions: Association between serum level of cystatin C with the markers of renal function such as urea and creatinine, as well as with different degrees of proteinuria, suggest that cystatin C may be employed as a marker of renal injury and preeclampsia severity. Disclosures: R.A. Costa: None. M.T. Peracoli: None. M. Romão: None. I.C. Weel: None. J.F. Abbade: None. J.C. Peracoli: None. doi:10.1016/j.preghy.2014.10.126
[121-POS] Maternal blood pressure as a risk factor of hypertension and hyperlipidemia in later life Kanji Tanaka, Maika Oishi, Hitomi Chiba, Kaori Iino, Hideki MIzunuma (Hirosaki University Hospital, Hirosaki, Japan) Objectives: The objective of this study was to determine whether the blood pressure level during pregnancy is a risk factor of cardiovascular disease in later life. Methods: We used the Maternity Health Record Books, provided for all pregnant women by the Japanese government since 1942, as a source of past pregnancy records. Of 641 identified subjects, 584 participants were included