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1247 Transient expression of the juvenile form of neurocan after brain injury of the adult rat
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The neurological and histological changes in the chronic compressed cauda equina of the rat Dept. of Orthopedic surgery, Chiba Univ. Sch. of Med. ‘, Dept. of Anatomy, Chiba Univ. Sch. of Med.2 Kiyoshi Yamaguchi ‘l, Hozumi Tatsuoka”, Koichi Tanaka2, Tanemichi Chiba2 We tried neurological and histological assessment for the model of the chronic rat to clarify pathophysiology of the chronic compressed cauda equina. After sia, a silastic sheet (0.3mm thick) was inserted into the epidural space. Pretest and the Treadmill test were performed until 24 weeks. The Plantar test between the experimental and control group. In the Treadmill test, walking the experimental group to 64% of the control at 24 weeks after the operation. axons decreased in the experimental cauda equina.
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Identification and characterization and up-regulated after axotomy CNS Research Laboratories, Shionogi &. Co., Ltd.
compressed cauda equina in the L4 laminectomy under anestheand post-operation, the Plantar showed no significant difference duration decreased gradually in The number of large diameter
of a novel gene (neurorep 1) expressed in nerve cells
KEN-ICHIRO UWABE, YOSHINARI GAHARA, HAJIME YAMADA, TOSHIHIKO MIYAKE, TADAHISA KITAMURA A novel gene, designated neurorep 1, was isolated by differential hybridization screening from a cDNA iibrary constructed from the rat facial nucleus whose nerve had been transected 7 days before sampling. In situ hybridization revealed that this gene was up-regulated in the repair stage after axotomy. The deduced protein, Neurorep 1, consists of 293 amino acid residues, and its molecular mass is approximately 34 kilodaltons. Protein sequence motif search indicates that this protein has an ecto-5’-nucleotidase consensus sequence at the carboxyl terminal region. In vitro studies showed that Neurorep 1 significantly increased the activity of ecto-5’-nucleotidase, which is considered to be involved in regeneration and repair of the central nervous system. Neurorep 1 might play a significant role in the repair process of nerve tissues by its regulation of ecto-5’-nucleotidase activity.
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TRANSIENT EXPRESSION OF THE JUVENILE FORM OF NEUROCAN AFTER BRAlN INJURY OF THE ADULT RAT Department of Perinatology, Institute for Developmental Research, Kasugai, Aichi 480-03, Japani, Graduate School of Science, Osaka University, Toyonaka, Osaka 560, Japan2 ATSUHIKO OOHIRA’, JUNK0 OZEKI’ , FUMIKO MATSUI’, YOKO YASUDAi$*, YOSHIHTTO TOKITA’ , SACHIKO AONO’, RITSUKO KATOH-SEMBA’, HIROOMI KEINO’ We have identified several proteoglycan species which are preferentially expressed in the developing rat brain. To determine whether there is any change in their expression patterns in the adult rat brain after brain injury, a small piece of tissue (L5.0xW0.8xD2.0mm) was cut off from the cerebral cortex. Changes in the expression pattern of each proteoglycan around the trauma were examined by immunohistochemistry and Western blot analysis up to 40 days after injury. Expression of the juvenile form of neurocan, a secreted chondroitin sulfate proteoglycan (CSPG), was observed transiently from 2 days to at least 10 days after injury. The lesion did not induce changes in the expression pattern of other brain-specific CSPGs, such as phosphacan and neuroglycan C. The expression pattern of N-syndecan, a transmembrane heparan sulfate proteoglycan, did not change significantly either. The juvenile form of neurocan may be involved in neuronal protection after brain injury.
1245
The neurological and histological changes in the chronic compressed cauda equina of the rat Dept. of Orthopedic surgery, Chiba Univ. Sch. of Med. ‘, Dept. of Anatomy, Chiba Univ. Sch. of Med.2 Kiyoshi Yamaguchi ‘l, Hozumi Tatsuoka”, Koichi Tanaka2, Tanemichi Chiba2 We tried neurological and histological assessment for the model of the chronic rat to clarify pathophysiology of the chronic compressed cauda equina. After sia, a silastic sheet (0.3mm thick) was inserted into the epidural space. Pretest and the Treadmill test were performed until 24 weeks. The Plantar test between the experimental and control group. In the Treadmill test, walking the experimental group to 64% of the control at 24 weeks after the operation. axons decreased in the experimental cauda equina.
1246
Identification and characterization and up-regulated after axotomy CNS Research Laboratories, Shionogi &. Co., Ltd.
compressed cauda equina in the L4 laminectomy under anestheand post-operation, the Plantar showed no significant difference duration decreased gradually in The number of large diameter
of a novel gene (neurorep 1) expressed in nerve cells
KEN-ICHIRO UWABE, YOSHINARI GAHARA, HAJIME YAMADA, TOSHIHIKO MIYAKE, TADAHISA KITAMURA A novel gene, designated neurorep 1, was isolated by differential hybridization screening from a cDNA iibrary constructed from the rat facial nucleus whose nerve had been transected 7 days before sampling. In situ hybridization revealed that this gene was up-regulated in the repair stage after axotomy. The deduced protein, Neurorep 1, consists of 293 amino acid residues, and its molecular mass is approximately 34 kilodaltons. Protein sequence motif search indicates that this protein has an ecto-5’-nucleotidase consensus sequence at the carboxyl terminal region. In vitro studies showed that Neurorep 1 significantly increased the activity of ecto-5’-nucleotidase, which is considered to be involved in regeneration and repair of the central nervous system. Neurorep 1 might play a significant role in the repair process of nerve tissues by its regulation of ecto-5’-nucleotidase activity.
1247
TRANSIENT EXPRESSION OF THE JUVENILE FORM OF NEUROCAN AFTER BRAlN INJURY OF THE ADULT RAT Department of Perinatology, Institute for Developmental Research, Kasugai, Aichi 480-03, Japani, Graduate School of Science, Osaka University, Toyonaka, Osaka 560, Japan2 ATSUHIKO OOHIRA’, JUNK0 OZEKI’ , FUMIKO MATSUI’, YOKO YASUDAi$*, YOSHIHTTO TOKITA’ , SACHIKO AONO’, RITSUKO KATOH-SEMBA’, HIROOMI KEINO’ We have identified several proteoglycan species which are preferentially expressed in the developing rat brain. To determine whether there is any change in their expression patterns in the adult rat brain after brain injury, a small piece of tissue (L5.0xW0.8xD2.0mm) was cut off from the cerebral cortex. Changes in the expression pattern of each proteoglycan around the trauma were examined by immunohistochemistry and Western blot analysis up to 40 days after injury. Expression of the juvenile form of neurocan, a secreted chondroitin sulfate proteoglycan (CSPG), was observed transiently from 2 days to at least 10 days after injury. The lesion did not induce changes in the expression pattern of other brain-specific CSPGs, such as phosphacan and neuroglycan C. The expression pattern of N-syndecan, a transmembrane heparan sulfate proteoglycan, did not change significantly either. The juvenile form of neurocan may be involved in neuronal protection after brain injury.