19° Congresso Anual da Sociedade Europeia de Micobacteriologia (ESM-98)

R!>V PORT PNEUMOL IV (3}: 327-364

19° Congresso Anual da Sociedade Europeia de Micobacteriologia (ESM-98) Lisboa, Julho 22/25, 1998

19thAnnual Congress of the European Society of Mycobacteriology (ESM-98) Lisboa, July 22/25, 1998

Resumos/Abstracts Auti-tuberculosis drug resistance in Portugal ML. ANTUNES '. J. ALEIXO DIAS1.Af. ANTUNES'. 11<1F. PERFJAA'.li. RAYMUNDO'. MF. RODRIGUES'.

Incidence of Mycobllcteri(t isolation in clinical samples from urinary tract duri.ng 11 17-year period from Greece (1980-1996)

Ml' MAR<)l !I\S' 1

lJlrcccio--Gcral da SaUdc LnstitulO Nucionul Ut.: SuUdt.: Dr. Ri~rdo Jorge Lubucl~rios- Porto, ' Administra~ao Regional de Saude de Lis boo e Vole do Tejo, Poi1\1!JIII

E. BOGL-\TZAKIS1, G. VRIONI1, A. SCROUBEWU'. P. PAPAGEORGIOU1, S. ANAONOSTOUa. E. MARINIS'

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Vol. IV N• 3

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REVISTA PORTUGUESA DE PNEUMOLOGIA

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S. BONORA, MC GUTIERREZ'. G Oi PERRI, F. ORUNELO•, F. FAVARJ", R. FONTANA', B. CONCIA, V. VINCENT'

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Vol. IV N° 3

MAW.Iunho 1998

19° CONORESSO ANUAL DA SOCIEDADE EUROPEJA DE MICOBACTERIOLOGJA (ESM-98) RESUMOSIABSTRAcrS

Mycobacterium SPP infection in three The epidemiological relatedness district councils of Setl'ibal - Portugal between two tuberculosis - explosions proven after eight years with the aid of 1 1 1 lliO
Secc;Jo de Mk.robiologia. Setvj~o de Patologiu Cllnh:u, llnspit.11 Gorcl• do Orta- Pragal. 2800 Almada Scrvi~o de l'atoiogi:. CIJn)O'I. HospitaJ Gru-cia da Horta

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REVISrA l'UR'I UGUESA Uli I'Niii ;MQLOGIA

Molecular epidemiology of bacillary tuberculosis in the Czech Republic

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19• CONGRESSO ANUAL DA SOCIEDADE EUROPE!A DE M1COBACTEIUOLOG!A {ESM-98) RIJS UMOS/ABSTRACTS

Epidemiologicul Surveillance in pulmonary tuberculosis (;AIIIUI(I,A KKAKHOI'fo:R. ALr-REDOR!BElRO.LUIS VICENTE 1:.1\cola Nacinnal de SaiJde 1·'1)hlica, f.ISb<\:~ l'ortugal OyfleGXIGf lhc XX-Ccntur:,Nmaar'l'abcrnlosinuU~·It<: I"Ml:r'~:n:nrc­ rwn~~· irl tbt NoM.wltb tco.kqutnets In l(mU of mon.ibt;<·. mor1.11~ JJIO .lbWI!tc:m h. b.a't.llf biJ. 10(bl ~t1turJ$i011 eftl'l'io!d •1\ 11\:S: ~!IOJ.JI;')I!' 1t1 CoolsetSt \~( (til !11.: nc«1 10 t!i!abli.'lh a pmgr.wn o( t4lid~:~q.juktgjull Sunillmxt in. Plilmllnlty 'flt.m:uii)'IS '~"~\(- '('(M$1(' pw~~' to tK lifiS\IJl'b:k:ol J\lt .an II,ll•:l q.ull,• ..,iJI o.m limo.' iu.llltm.IIJ:ul nnd !~t•:!:- ~oek !\lncc 1~.:­ tl~l ofll'i 5«~ "'t l'll.l.IY~ 10 tSI.\Oii.ih 11\l~-IIISIJI!.f.ICWI ful~ lOa bttll:l ll..>.:it.'ot O:I!UUa l We obuintd thcrdM ajotru ~OQ of Ult JCr\'itel fq.r ~ beel<:r Ple'lti!Wu. ~!~~SIS. I.Jt>~l.1111:ul D1 xci:al rdtlli:Sfl'OOIL

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Muio/Juuho 1998

Voi.IVN-3

335

REVlSTA POifl UGUllSA DE PNEUMOLOGJA

Synthesis and antimycobacterial activity of ncosynthcsized 2-pyridincnrboxamidrazone derivatives Antituberculosis drug toxicity io the pneumology department ofEgas Moniz b.. HANFI", MG. Mfu\10LO g• M. PREDOI'
• Uipartimcnto tli ScicJU.t.: Biomcc.H..-lu: f Uipartimcnto dj Sc:lcu21: Fonuu~utiche. Uni-.•ersitit d~glt Studi di Trieste 11 Mol.luJo di Microbiologja PoJJnonafe Ospeda1c.· Stmh'.lri(), Trieste, ltalio '" J"VO;.., I'"('U' : l ·l )

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Vol IVN•1

MaioiJwu>o 1998

19• CONGRESSO ANUAL DA SOClliOADE EUROPEIA DE MICO.BACTERJOLOGJA (ESM-98) RESUMOS/AHSTRACTS

Is 6-montb anti-tuberculosis Mycobacteria and safety in chemotherapy enough for pulmonary the laboratory tuberculosis in patients with diabetes MAI..UILM OYATES mellitus? WA.DA M. OGATA H, ITO K, YOSHIYAMA T, MIZUTANI S, SIJ<;JTA H ln.•lltute ot' litherculosis. Japan. Aoti-ruberculo.si.s Association, 3·1·2·1 Matsuyama, Kiyose-sbl, Tokyo, 204·0022 Japun \\'ltUIIdl.ll:ted a lftrtlspectiYI! ~ &MI}'tli to ddtnnl., J611>11nth slari ooune r.hr.mothcnpy

(2HJI.ZS llf FJM 111111 97 K 'M,J Cl.iiN~ positive io clto1c '1\'ilhwl. U).t Ni• peraen1 nf Q.6QC hld ~~~~- kinds nr MYtr~~t ranloe ht. tlotillfOUP. Con'l'llltme fllte 11111 no.) m(Jfllht ot dJMIII~IIoJIY Wll' 1\1%,. 11111! 1)\% fttpetfi~ly, 'fht ttlip.. till! '"'U 101 % ('..,~,) ;II patleol1 wilh D\1, 1md l l h ( Z ~( II~) in pl!it.ll4 wiltaoul OM (1'•-:
.-w-

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~I ~~ and tbt st-:o•ld by I110wt.I.•On vr Nben:ukn" mmerbl

Silt()' proc:er!ura .n therdore d~.d 10 lltnl1 ''- p:OJdutlice arnern,nlt • nd 10 ptt\'(fl! wor\:m illh~ lbetc ~u, • n4 aeo:CIJ'dl}· ~ ~pr\1\hMnmvr • W<.ri.ins mmhl)d thllt d!tl'df'IUtt

&«ide:nlal inoc:lbtiona, There m thrcoc Jlcps to.,.'lfllt the ~iii'.JIIai!OII t-1'uolf&.'lu:a by acnnd.1·. llrvl)· 111(- lti:MIO!'\' 1\it to be dtii~Jitd i6lK.I t~f .irfl~· wilfll,t U~t (IXIJII 'f'lll .et"a~e: all)' U1nw.l,; r,,,duct,(ill\lle\' ftOI!Itht .,..--oNt•l. S~ly equipment flu 10 tit ll"'ii"..J .ad ~~~ m sudl a "'liY 111111 'I'IY acrotolt l)r~oxcd areenmlll)ed •nd cannot ftaeh tbt wottt:t, l hir.Uy tiM! "''-'*bt lw to be tninrd U) WOf'lr .tdy. nd P'*" is al1(111.11111$M\"ffltllc the elimination ol ~5f!lalll,.,.;uhtllt:aS Ovl!!ll!l r:m.v lh: lftottt~· hubetnd«lsi!Od jli'OI)tfly. «fne~:l •'IUlp:lltlll "'"'*~ •nd u!~y proc«iuttf 1 ~11¢11 lhtfl tN- "'~~·cntion (l[llobQ(•t~) ~(11111~ uolfau:n:. n in IMIIAndt
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against mycobacteria other than tubercle bacilli

• Uni\'crsi[}' ofteip7.1g, Institute ofMedkaJ Microbiolo~, Germany ... NutionaJ Reference Cenr.er thr Mycobac.teria. Reseun:h Ccntc.:r of Bur.llcl, Germany

R. BAURIAUD', C. LJLit.MAS', S. POMJES", L. MARTINEZ',

H. DABERNAT Luboratmre de Microbiologie, Hopi~!\! Purp811, 3 111~9 Toulouse. Frnm.:c

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Maio/.lunho 1998

Vol. IV N' ] .

337

REVISTA PORTUGUESA DE PNEUMOU KtiA

Susceptibility testing ofM. tuberculosis by the MBIBACT (Organon Teknika)- a multicenter study BEER J <, G. DAESCHLEIN". K FELDMA l'fN3 , .1 .
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Natural resistance to claritbromycin in MycobilCterium tuberculosis is effectively reversed hy Sub-MIC concentrations of ethambutol S. IIOSN£-llA VIII, C. I'O
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MaioiJunho 1998

19' CONGRESSO ANUAL DA SOCII!llAllE EUROPEIA DE MICOBACTERlOLOGIA (L'.~M-98) RESUMOSIAIISTRACTS

Drug susceptibility testing of Evaluation ofMBIBACT for susceptibiUty testing of Mycobacterium. Mycobacterium tuberculosis using MB/Bact* process system (Organon tuberculol·is Teknika Corporation-Durham. USA) M. GARRJG6. ~.GARCIA, C. M0R£NO, P. COt.! ,

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C.....:'i..l(l
Using mycohacteriophage D29 for rapid detection of drug resistance

Laboratory diagnosis of M. xenopi pubnonary infection-one isolated case in a non-immunocompromi~ed female patient MJ. SOUSA', PJ. PINTO' , MC. GOMES" , Mf. RODRIGUES'

lnstit\lto NacionaJ d(~ S~u)dc Dr Ricardo J org~. 'fuben.:uJose c Mi<:ob.'\crerias, Porto, Port11g:-~t 1\'l.IC. SALL"S, It MeN~I(~J:'Y, NG. STOKER

l...;tbor~\t6rio

de

•• <.:enuo de Diagn<\strco PneumoiOg1co da Alameda, LJRhoa, l ,nnu.~al

London School of Hygiene&. Tropicul 1'-·l•.:Uidm.:~ Lvm.km, WCI E

7HT, UK Hct¥.}i~ . ....iu.~~U~t~.JolU..ptiblcn~ u( .... tmilltritcli.ictioo.'* ~ W• ~ drnund fnrmi)MtvlfiX diC drutt "'~,ilil:y ol,\~- ~ltJ.ri~ ~. ~tolctu.lac ta~:lhods and~ tiqui4~CIIdbcdlofbet~tt ~~tna1bcir ~~~ eo\1 lU)J U. ••ucn:alll« !lli(diliau:alal ~ !i.JUI tt.it applir;acioo i.G die poocty mnJn:cd t.~ bUllS ifl P\IIMIY~ ....nil a hi31J bunb! r:A~. We dc.monstrwtbc uacvt ~plgp 01~ wlkla1~ol ~ria to

n llunpn:u,, 1.,om.u:ld, :rtftt!tn!W)'Cin 11M t~bombutol We ~ the inhil!iblllt arplsap

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m...

Maio!Juuho 1998

Vol. IV N• 3

341

REVISTA PORTI:GUIJSA 08 PNOUMOLO<.iiA

Radiometric broth dilutions method and e-test agar method for clarithromycin M ICs of M. avium complex, M. xenopi and M . .~crofulaceum clinical isolates

Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis clinical isolates from Greece ( ;. VIUONI 13• E.ll0(; 1AT7.AK IS2, E. MARfNJs'. 1'. MATSJO I'A· lii:!RNAIW 1

l\1.1. SOUSA, EM. MAili'I:-.IS, MP. llOUJ(I(;tJI'S

1

Lrudituw Nu~;ionul 4.1c SuUdc Dr. Ricurdu Jorge, Laboratbrio de Tubetcu1osc c Micvl>w.;l..:rins~ Porto. Ponugul tlalillllOIII\(11. II 411 U!~llllll. kuil II N~~::/.'lf:lf:lll 0111• Tb• Tllbfm.dI)IOTn a..o\..'1i111U. Sm:t lhefll .ue no tsuib\stui IMI!Mt fbt Mos !ll'ttp'•bilty tUIIIII et fhtv .vpnm~•, ~ 5h.ll•""" t....ou.. >UC """ ...,, ~nl ..-...,. ~>~ . .n dn:v \1Qilwt~t-.- • • fooJ!d na10 b. 11'11$ili\oc ~~ 10e.G~Gc: a±::m:aiWI ~ a.el•~t~~

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Laboraloil\! de Microbiologic. Hupital Kaymond l1mcare, ()2:lXU Garchcs, f runec, : Reference Center for Tuberculosis . Laboratory of tv1terohtology, Chcslc Diseases Hospital of Alhcns, Greece Tbn• is • ¥"'VIP~ Wwibllliull tf 7"8 ~PO mullllium in .\ ('>Mhm:MI'fv... tldtii(W>'(I..t't IICCO!IIIhlll ~, nf;liiiP,'I ~r ..:I)IJ.:mivlcfi,P:il Slll\llc:,. blllll~l O:. .IIIC 1\'~~ "l\h I~ uwllr.:ld ;IQ lll.'t N ICOIII riF.I.mf'in ""'-''l'lr.f~

!.IAI jllatu\"'

Isolation of Mycobacterium tuberculosi~ Use of Line Probe Assay (INNOLIP A) by not radiometric system in n for rapid detection rifampicin Universitary Hospital resistence from clinical specimens .W.SfiS de La CA LLie I, l't\Rt!.l..ltAMOS S, RODRJGUEZ IGLESIAS MA. U)PEI.. BARBA J,

M. TRONCI, 1'. C I·IIARAI>ONNA, S. NA 1'11.1

Oepan:uu:m of Microbiolo~.y 1 C Forhmini Hospital. Rome. lcaly 41\f t•>lt:.a~
JESUS de' Lu CALLE MA

Servtce of M1cmhtology. tJnivel'sity llos.pital Puerto ReaJ. Real. Cadiz. Spain

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142

Vol. JV Nl< 3

M :~icvJunho 1~8

19' CON.GRESSO ANUAL DA SOCIEDADE EUROPElA DE M1COBACTER10LOGIA (ESM-98) RESUMOS/ABSTRACTS

Evaluation ofFASTPiaque TB for the rapid detection of Mycohactrium

Bactec 9000 BM: one year of experience

tuberculosi.(! ANA PAULA CASTRO. ELSA CALADO. SANDRA SOARES

DHIR VK., HE YDENR Y C ~l A, ALIIEI!T II

Service of Microbiology. Hospir.)l ltw ~ ,_,J UNSIIit \kOJ&UC,t ol \f)~'"

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BIOTEC Laboratories Lid., 32 Anson Road, Manlcsham !-Ieath, lp5wid~ Suffolk. 1P5 J RG. UK F 1'>7PIIII{UI! I H •~ a l;qJII! .45~ ~;~lc of d:tc.:ciog .l0"CQ~.Xfm:col ."li!:olu!do:fis (~fftl} ln. .&8 ....,n,.l rmm ti:CI!IP: •>l ll:llnj!l~. All ~I J!U~IOil of th.vly u~ tal"~:l!1111 ul :>.llS e.1 .J.• .,. .:.:~ Oao;l!< :Dawn. L)l~k1ftj,JIII o).lrnl B~~ .nol \79~ ,.., ..11n 11y. r.-.r,.cnw ly) f 1,\ !PI~u:TD iJ a ,....,.•.;t. r~!lt.:l, ,.•11sl•i\~ ·•r•••!ilie nnol u'lllt·l'rir!'1.1ly \ITA d1'1'1'r nnn -.y~m

Comparison of two fuDy automated 1ntegrantig the MB BacT rapid culture liquid culture systems for mycobacteria: system into routine diagnostic use bactec MGJT 960 and MB/Bact preliminary results KEMP M . HARRJS c.;, YATES M , ·oROBNlEWSKI F PI II.S Mycol>.,cterium lteterence llni1 (MIW), f)ulwich Hospital. London Sl\22 MQ~ Evan~clisch-Frcik.in.:hlichc~

K.n:mkcnhaus Bcnntu, Gcnnany

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MatoiJunho 1?98

lletwecn May 1996 und JWJe 1997 the Orgillloa-Technika MB IJJil:T non- radil<:m llllhc PHLS MRU. nu~e different dect:~nlamination systems were used ror I'C£pirutory sample>. A trial comparing MD etilrute bottles with or without vancomycin supple:- mentation was also undertaken. The three methods (quoting initiW 4:0nCC1ll.tatiOI'IS)were 23% trisodium phosphate (TlUI'),2%sodium hydroxide(NaOH) wilh N-ocetyl C)'Steine(NALC) :md .J% NaOFf..NALC. Positive isolation rates in 1h..: ~fB-SacT JOr .11lmyco b.;1cterial sr.;ecie.' were 9.3% (86 out uf 927)wilh TRIP, 9.6% (9 out of93)with.2% NaOH-NAL.C and 7% (13 out of i8S) with 4% NaOH-NALC. Average times to detect all M. tuberculosis isolates using !.hex dc:contaminat.. ion methods were 18.$ d.>ys using TRIP, lOdays using 2-.I.NaOH-NALC and 18 d.>ys using 4% NuOH-NALC. The overall contami~:~arion rates in the MB OncT w
Vol. lV N' 3

343

Rf.VJSTA PORTUGUESA UE l'NEIIMOLOGJA

Clinical evuluution ofMBIBACT and compnrison to solid media for isolation of mycobacteria from clinicnl specimens

Evnlnation of two liquid culture-media (MGJT and MB-REDOX) in comparison with Bactec and Lowenstein-Jensen

V. LALANDE. S. FAUVEL, C. PERRAGlJI\, S.WADEL. JC. PETIT

t:. MAIUNIS'.E. H()(; I.<\I'ZAKIS 1, ( ;. VIUONI', S. NICOl .AI!', S. ANAGNOSTOU 2, A. SCROUBELOt:2

Department of Microbiology. So.im-Antoin~ Hvspltul. Puris, franc~

• f!.·ticrobiology Laboratory, ltfi.H. «l'olykhniki>>, Athens, (irecc:e Reference Ccmer for ruhcr<::ulosis. l .ahoratory of f\·ltcrohinlogy. Chcsl Di!-!!ca~c~ Hospiwl or Athcn.s, Greece

2 lite '-U:I.'Q.ct 11110nuu-d. f)"fl:e:m, ll ttl:n 1.111 I (\:IIII'II:IU!ll.a onl~lric em delaltiiWI deoine by mew ola 'JIII}I! <;tnl.e ~<;nr ntlhe N:ll'l n( ea.~b """lc. We C04'11PIIml the ~ o( r-(D.tllcl syJ!nn to soiid mcc!.la loe·.rt.u:doJtrurn (LJ) {i)i~s ~II(') Nc ~tioo ul m)tuba~:ktU.. Noo st.cuJc ~p~;ll:l:O"'' WC(o; BacT bllrt'.e (jOO Ill) ntvl (ii) nr lrn)l oa 1 U "l~nu (4001•1), For ¢tr.t~ll"!innrtd ~~m~. llo(B.
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19• CONGRESSO ANUAl. DA SOCIIlDADE EUROPEIA Dll MLCOBACTERIOLOGIA (ESM-98} RESUMOS/ABSTRACTS

Evaluation of an automated system (MR!BacT®_) for isolation of

Mycobacterium spp R. MORENO, A GARCIA del BUSTO, F. PARDO,JV. GALIANO, S. SABATER. L HEIU-IANDEZ AGUADO

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Comparison of the BACTEC"' MGIT'" 960 systems with solid media for recovery of mycobacteria from clinical specimens A. ORTE(;A, J. MARCH

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Evaluation of recovery and time detection of M.. tuberculosis in the MB/Bact culture system compared with micobacterial growth indicator tube (MCIT), Bactcc 460 and Lowenstein - Jensen medium

Comparison of COBAS®AMPLICOR MTB automated system and smear microscopy and culture in routine TB diagnostics EMIL PAVUK 1, MARlA M(lLumov A~ MAREK BEDNAR ! EVAPROCHAzKOVA '

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Evaluation of the BACTEC'"' MGITtm 960 system for growth and detection of mycobacteria in human clinical samples

S. Ri'JSCH~;F.RDES 1 , A. EtlRAHIMZADEH'. LB. ELLIOTT'.

AW. STURM, L. le ROUX

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University of Natal. Congella, Soutl> Africa 1

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MDA Evaluation Centre. Public He.-thh Labomlory. Royal l.ntinn31)', Leicester. UK Puhlic llealth l.aooratoey. Birmi.ng)lam., UK n...•rs.a.:t(~T....,ob\,a oopol

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The subop(ir.ul t.o:r.J~illvi9' of dtil lmmunocl'.t«~~JL\19l1Phiu II!~ lulfliel ~bill il c:uurm1 be " f~pl:..:en'lCI'It fw lhc "'fflll r.~il:lllli mu'fN1n8 &ten tubcKulotis.

Thca_WIIl_,_,_•M-tJfkd ft.lt~~>nioa•)'(l
Vol. IV N• 3

Muiu/J wthu J998

19' CONGRESSO ANUAL DA SOCIEDADE EUROPEIA DE MlCOBACTERIOLOGIA (BSM-98)

R.ESUMOSIA.BSTRACTS

Usefulness of the Accuprobe

Clinical evaluation of the amplified Mycobacterium tuberculosis complex Mycobacterium tubercu/Qsis direct test culture identification test (Genprobe, (AMTDT) San Diego, USA) ll. van SOOLINI;J(N, 1'. Van der L.AAN, .\1. DESSI'.NS

I~)SI'll-!.C I·l,

M.

Mycobat.1Cria dept., Nal lnsl. of Pub. H.:ullh wtd EnvirormlCnl, (ltiVM) 1'.0. Box J, 3720 BA Bilthown. The Nclhcrlwnb

LOTHAR UfNilER, MARJANNI! HERLBAUER. HELMUT MITrERMA YER llqlonmemofMed. Microbiology .00 Hygi<11o, Elisabelhinen Hospital. L.inz/A u..,tria

·w•."No:"- In !be

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t~.•.bt>r;~o'l\uis c;omplex-speofic inwrtion seq~.:ence ISGJfU, M pr¢'1'9n in reslne:ll~ ffi~Jm•nt I.,_Qlh potymQtPh4m1yl)lng. The •lftepbonalliotate wlthoul l ::i61 to DNA wat ld.enbfled u !l'.l~.u'!'ow Ofl basl6 ot other genetic aM {lh&o~t)'poc

conl.lned It,. M.

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'v"' 'Joo~;tta•n, o., P.I!.W. .,. Hut, I' w !Wl U•""'"'""·MA. G_.,., ~nil ,J.UA. v11n t 111b6tf\. 1\l\13.

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w't.en 1tte cuiii.U cCIIIt A..\(J'(lTwrft:.o;ecrciJ!«i •llh notoalrW culon ~'utu, but also 6e-pJt:CIIl hi~ II\ ..t il " ~~ clil'oil-.11 llld thtfl('elllic iniunn•inn, me ,QCI'DT rt-111h5- ch~ll '" fnllay,~: lttt• 'N.¥'it 10 U.l~

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d

Bacteriological diagnosis of tuberculosis Detection of Mycobacterium meningitis using the BACTEC 460TB tuberculosis in clinical sumples by DIG-PCR Elisn system system ~1.

VIVEIROS, R. BETTENCOURT, J. ALMEIDA, T. VENENO,

L. BRUM

Labonu.Orio de MicobaclCrius do lnstilulo de Higicnc c Mcdicina Tropicol (UNL,) Lisbrn~, J'orlugol

M. BORUN', A. SAJLJllllA2 , I. PAWLOWSKA', J. DL.IADEK' , JJ. McFADDEN' 1Ccnlrc of Micmbiology and ViroiOID't Polish A~;Udemy of Scicru:c:;, Lodz, Poland 2 Dept. ofGeneL ufMic:roorguni!;-m:;, Uulv. oflodz.. Lodz., Poland ' Lung Diseases Hospilul, L<>d<, PoiOJ:Jd ' School of Biological Scien(CS, Univ. of Surrey, UK llll"nllMIUMudMit il dlr:IIIUllmpo:mm r~elul utdhttil!ll!ld!ltlll~ ~ld:ulnoadl~t­ Tuh:.,.dnlh hu l!- 1hereumd1U'W"'' ~of!lt.m uwtdl!)' lllf«deois.!II~IS. ~~~~()(.\r ....o:.,...,.(,\.u ' 111116dau tpK:tlltN h f'e-?ltt.i tW lbt lld!nhlw dl~ls at ~.lbmule5ls. n~e ~Wfl n!(d tulllild ou"lllwll)'lfli M. e~(.uis » lilt !(l«hs leYd It din);:d ~ iJ die PCil l'llnllll olour 101d. •u WI e::.ctiW!

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MuiuiJuolJO 1998

Vol. IV N' 3

347

REVISTA PORTIJGI IESA rm PNBUMULOGlA

Isolation of a Specific DNA fragment Amplilled Mycobacterium and Development of a PCR-ba~ed tuberculosis test for the automated method for f'he detection of VlDAS instrument

Mycobt1cterium genavense UAN II:.I.c UIEV I!Ihl!, GAlli! II lLA OPIUSAN. 1\NAI:.NLA MAKI!.SCA, l't;( ;(;y MATSI< f rA-nEJ!}IARO. JEAN-LLJC GUESDON lnslitut P~tcur. Labonltory of J-rcdcvcloppment de:; S()ndt'Ji, me du

OJ'. Roux, 75724 Purl:;. Fruuw R. Poinc"re Hospital, Laboratory of Miorubiology, I04 bd, 1<.

Polncore. 92380 Gatches, FrMce

CLARK-DICKEY K, 1, BURNS J. 1, IIASSEI ,KUS-LJGHT C, 1, LONGIARU M 1, L.UOO L1• QUIGL£Y'1 1, SANTA ANA 5 1• SIT/IV A., WEL"iBAUM B', McKINLEY G'., MOE .1 1

<;en-pr(lbe lnc.orpornted, Sun Di<~u. CA. USA ' btoMeriex Vitek. Rockland. MA, USA 1

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P. DELLA-LAITA. S. WHITTIER Cohtmbi•-l'resb}"erian Medical Centes, New York 1kdlm:t acpimaao11 ~u IIJA1) u ..r Wlir co 9:0M $1, 'ookmoo'wi•·wcoc*a (M'J'8C). Dill ..J)· fn:a ........l)'. ~lf lllll)l'ft.M (ll~ fPI!(:ioJotru.. AKCO!Id eucntioo UAT.~ ~lim lullen:Ml.~it Dim:~) l)m':ll) T~ (~) bM lwl ~ wa~to\bc ~..w;&l ami w.lhlf9kll ~ u(MJ'BC fn:oadi;sed llflddtDirnamiiiJIII:d~(ll'y . .illtcfd, l.'rd!UIJIS'M1VIIat ilut ~ ~ .... urlillldimenl, the MID) fllillll:'d&~ T1lll fl.e 111

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348

Voi. IVN• 3

Maio/Junhn l <;lq)>t

19° CONORESSO ANUAL OA SOCIBDADB EUROPEIA DE MJCOBACTERIOLOGIA (ESM-98} RESUMOS/ABSTRACTS

Development of a quantitative diagnostic procedure for Mycobacterium tuberculosis infection

Utility of Polymerase Cbain Reaction for the diagnosis of pulmonary tuberculosis in a rural african laboratory

L DOLZAN1 1, M. l' llF;IlOMINAT0 3, E BANFI'. M. COMAR' . C.

M. Kl:VIANKINDJ\ 1, C. MEE1 ' . G. MBULO' . V. TIHON".

I.A.C.:ATOLLA 1.E

FAOIUS'. M (;JACCA'. (' LIN'. C. MO:-.JTI-Ill~AGAillN'

TONIN 1.L.ZENTI

IL :VIcNEIU'E.Y' 1

I ) lparhmcnto di Sc1cnzc H1orncdiche, 'Jm,•en;.1ta di T1't..!~1 e .: l ntcrnallm)al Center fi">r (•cnctlc Engmccnnf2 and U~ntechno log_v,

Area Sdcncc J-lmk, fricstc :; Moclulo Ui r-.·ticrobioloyia fJo)monan.:, Ospcdalc Samorio, rncsilc,

llaliu Wt, l"" IV •
.lioo..oJ '"'"1~... n..llco."l,.,q...,. .o boti
"'J!"'" (>I IU outlliM tit_....,, ~fil Ill, o~'o: 1u .., t.01I WD(I~. Clltl~ll'l: ~ Vt'IWi M~lllltud ty lllwlllllt. "''l'"'"""• " - M ~~~- rudloll"> "1 f!J t.lulnJft"''"'" n<:~

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toMtn M ""'l""'*'~ •ill
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St Francis' Hospital, Katete, Eastern Province, Zambia ' Z.:\MBART Project, University Te.:tch.iJJg J lospil~l . Lu~k~. l'amhua 1 London School ofllygi~ne 1<:.. Tropir.:~l lvfedicine. l .ondon, IlK llkfl: J~M bcca II dnmatic; rile Ul lbe iD:i&IICC' o( sm:aNKptiw Nbtmdolis in ~ A.r.-..:r~~ll.'f'i&lltllcHJ\' p.AI•II&tl'li'. t>.orioa 199Sfi1~ (lt, l19Jollllrl' oo~fA 11ulnwMfY tabo:n:ulo.tl111m l ambua dtd niltllll...c• poii.,\'C ICI~:W.l~L We b.t•~; bl.x::a in\ lmit,.uing 1he mlc Pf R a~ald plar m d!agJ~•lllis ur pufaun:lry 1'8 in a roW brola&uf)· a.nrcdlt ~n.e,&r;:r~ 7N ~" .,.,hrm filcihri~ m culllum.\f r!Jh.ol't!llltA\IJ Mltn: 11.\IU'bhlr.

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Clinical trials results using the enhanced amplified M_ycobm:terium tuberculosis direct-(MTD) test

Co-operation of clinician and luborutory in interpreting results of examination by MycobtlCterium tuberculosis direct test

V. •11 IN AS, M ACELXJ, 0 ..VI ONI ANO, K. SMJHI, G. MATHIJWS

.IAIU\11J.A KA IISTOV;i

Gcn· Probe lm:orpor-a.Lcd, San Djego. CA. USA

l(cgional ln~tinne of l lygiene, nep:.rt.menl tOr r>i.,enC't.~tit:.c: of

O hjood, 'l!!lr T u dcu!rmine the pelfammnn: ar !he ~a:und r;em:1111ian MTO leY by compari:ng rtJulu of !be~ '6'1111 pMkM dlag~tosb. .MdbOO.; A f.iolpbfJcd ..~io!l of 1!1c ltltD t.¢:51. with fewer 11epc and ~ &horw aropUflucioo th nn Ill'&-~ a\Cd kl '1:1& te~pmehll)' \CI!irn.:en IC 7 dit~iCIII tJilll Ai1n, iDCiucltn,s 6 in ll• U.S. and

w

ooc bl Sv.•luedaod. Pa.rktm tuJptn('d olbiiVIO# ro\ll:n;nll;'l'lit AAd GOl recdviag tbernpf ,.'en': CJICOikd in the J1\.ldy, from J.(j wnpkl per patKat •~rc tcs1cd. Skwltl of the MT0 t«t -w.:re a 1mpun:d w11h llu~ 11ile phy~k:illtl'!'l ctiagna~i!l. AO.Xllllill&lu tn.Ublultod cnkna. QIL'Ies wblcb 11+is ,..,,.. 111.1'.\, W,6._ \18,3'i!:F iUIJ ?S."''
MuioiJunbo 1998

Mycobacteria. Osrrdva, ( ;7ech Hepuhhc ln. I ~S. 1Jlt Otp6ltmt1'1t far fl.tyrobar.~erillluguP\I.n:~~> ul Ll• Ktv,ivoa.l JllftiMC' of II}'Jiene (JUt ~) ill Qs1ra'll perJ"ormt<< Ot&ll'llr.ttion r>f ll'l 09 'lllmp!e. !'IIIIII.IIIJ,fll'V!ol)' b)' Cl.lh\lre, MT0 ,,.,. !Uld, ._,~ fO" wine sarnpks lltd LV, 11i~ micr()'Wll!piclllly In j0 11alnmtt1 will! nc-s;tive ,_,In, '""•hxll b> clusiul 1D¢1bo4s, 1. po~tl'oe Min r~ ,.,..., ful.lnd !1' af It - p~~~la ~~ocre 1\nnil> dW¥•10-.1 as bl\ 'ill8 pulmenwy Nbetrulolls al)d 4 cvtr.l(flllhnnrwy lul~~!! wl06ul, ia b«h rll\6 Wllllm•.em•f'J b)' b•cterioloiiul invo1i3t~Soo.•.uothtr 4 pm5tnrr. N ve l:lel!le lfl&_!VX!scd 11'1 A 15; MDKdu~ tO lhl ltll~liolll.l ( lwif~<:atioa ofDht~t -1'8 QOAfirmcd b~· otbct rntlbod' • 11'1 tkis Cll:we hy ~nu ~a~t. ll\ tS ptlicn" •~1h positive MTD ttfL the

1nendin:s pllv~en ha~ n•lor:l Otrl TR •t'lil•k•gy Ul'lbr di~.

All paliocnls rmllllt~td fbnbet follo~o~ol'd hy 1he Spaul1-.rll «
Vol. IV N' 3

349

REVISTA PORTUGUESA DE PNEUMOLOGIA

Comparative study of PCR and LCR methods in the diagnosis of tuberculosis

Evaluation of PCR amplicor

Mycobacterium tlviumlintracellulare kit for the diagnosis of MAC infections

LOPEZ. L. VITORIA A. ' CRUS ELLS MJ, RUBIO MC St'rvicio de Mictobiologia. ' Servicio de tinfennodadesln.fccc.ioSlls. HMpit.li Cllnira, 7~mgo7,,, Spain ~ n.

J. OI.IVII :R, M. CHOMARAT. F. BREYSSe

I .al>nrotory of BacterioiOI))', Fuculty of Medicine I .yon-Sud. Fmnoe

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Evaluation of the LCx MTD assay m pulmonary and extrapulmonury samples

:\1ARIA M1lLLf.IWVA

E. PALENQUE', MJ

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Ma.Oi.lunho 1998

19• CONGRESSO ANUAl. DA SOCIBDADE! EUROPeJA DE MICOBACTERJOLOGIA (ESM-98) RESUMOS/A11STRACTS

Comparison ofPCR, culture and histopathology for the diagnosis of tuberculous arthritis

Efticiency of the ligase chain reaction test (Abbott LCx) tor detecting Mycobacterium tuberculosis complex in clinical specimens

MJ. REBOLLO, E. l'ALENQUE. P. CARRI:JRA

Microbiology and ReLUnatology Oepo.nmems. Ho:;pilull2 de Oc1..ubn.:. M:uirirf Spain AIM: 'fU IIUiil) (If PCR fM 1'-t di"t"Mii. 11f nd•m lllf!OII Ull!tti• ""'' 00~4 will! ((IC~Iopd ,..,.ibc~h.

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Serv1t;.0 de P:uologi{l Clin.ica (Labomt6rlo de Bact<:riologiu). Hospilal de Santa Mana. l .is boa. Port\1gaJ Pllf)IO~I E'4hmlun llf 1M "W.U. dl.w no11.1011" {lCit) ~ 1ft lilt ~bit diapo.b e~loyU.!t;r"m-' v.dmnop.lbolw ~Itt. , dltp~ ~ th11 pesitivi!y in 1..&-uuho.JmJ~ (L-J), 1J ..,..,,. atilwf~ l;ice"'Co=l JIIIUL"r lAd ftbn.uy lllll~. (ifQU 1!111 'IJ ~~... ~) _,.., jlulm- oritpa .-J-ftiiNDet Wl':tl'l t..m .~ !SKi~ wu INdc tllfiMm*"t til tilt M""lt"' us~ Zicbl.,_lse. Del <1¢.: -.:oiiKO(Ie t:umill.c.oo, ~ ~Ia "-'lamlllllled ..nd ~eDotiaal by N lk..:ildwe:ie • md•lf ~llllill"~ '11-.r.'t ftiiOIIMIJI)'fO!RI)V~oo fOtllt-~ ta~o..lw T""u altqlltU'IIII wiAI ~lt....-m: L·l 111111 .ntdler b U:a p-.-'lrt.& ~ -111"" ll!f1'11

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LubunllOI) of Mic'n,biolog)11 Ospedale di Circnln .1:nd University of Pavia.211 00 Vun:s.:.Uuly The dfkimc:y
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ROSSI A, COLi A. CA~TARI:lLLI L, MACCAGN I S, llASSANI A, ELL<\ V, TIBILETrJ L, CONAI.UI I'<; , J'ONIOLO A

Mft!IIIIC$, Alltl~te ~tflhtly WU 9S.1%. Tbt d!idmcy or lCx _, 99.1~ wilb Cllc foiiOWIIIII l'lllo.lbud wJve.! tc:MitMa> 1».1%. ~Uklty JO~'- por.ith~ prtdio:ti\IC to>tiiiC ?1,.,.., ~~"~ puli~Siu w,loc ~.2%. r. die (UC ol onnlpU)alorwy suopkt., o:lllnu 1¢1'1Aiivil)' ' " lo\Ost, i.t. ~. La.d!Sdmcy wu 96.6% l'ilh the fi:iiOII>'ina ulwlt~ vai\IC$, w:it£ll11-:il~ 1\1..5%. 'f*ificll1 100%. poehivt Jl«dicthe mue 9U%. noepti~~tc pt'Niai.,c
MI*Mraia..

Prospt,'Ctive evaluation of Gen-Probe's 2nd genenation nmplified Mycobacterium tuberculosis direct test G. STITtNfMAJIIJII, M. SC I IOLKMANN, A. AEBJSCHER-GlJJ{ J'NEJ(, K. Mi'!JJJ .I'MANN, T, IIODMER

Univ, B~m. Clin. Microbiol., Bern. Switzerland --~llltllldJ.'The~el~~ P"CWorionGflbt<~N~u~tniiMII'fllilq~MDhn 'fellfAAituf.J,t,;o.PW"-.S..JMJM,CM~IMdina~nniiiiN)'fV'lM,_._~CMibJ

.,.,., ~vllllntd loy""""' ~wtly colle
Me(,fllft: 1\3'~lP«illltM oC?(W;ptb«lb · ·e.c ~by lht Wldlum4ocb:c)i~ ~ b}tdruddtl!ledlod, llf:ftll'Nd 4bt at:ld.b M.:llli trt 1\oo"K~Ill!t owcooeopy,IWCI a.l!Uitd..,..

Owcc Jlll llldl..tMmt~~ll*liwo. &.....oiO..xdlm--~ ~nttofbe: pi&llllt:lllfdle IMNit~turcr. Alrlpb.ficttiooe fUUits .,._ -.,.;oJ ~ liMM 'Of'\Mihn.

ltatlrst l't~)'icldtodMtb(tnqlmccl..S%).$orfV _,..., li-*"~nallrd 11t be q.~ IANn &.ro.!Uiti)W . . tD~M. ('~ l(lalln~~ ANTDT-2 tod flli~KtWiiloda~olYI.4'\lllll17-4..n..~li....,;~-Kl%wltt.tM., Rliptlllh'tf)'.

c...- .u.rTD'f.l-'"'faeMI!w 'n.._..Mlb,ud~WC.. boe• powcdl.i ll;l(al w-..a. llfUtii(C411M4Jin ,..._....,.~of~-'lnne411f*IIIIMI ftwMI&, .n ..r.wa o:wici a trt"-nt~~.nd.t)· -fllml,_,.~w~n-tnisKd.l.tbumury ~-un~IN:Ii!a y.\'JI'b me)rinlill •llbrlpriflllf (!ll'fii'OM!t!fi'AI (Onj i!ionJ protably WCIII • C;)JOI «aali ob1111,u Iii 1hc WIIWiiiiiM"- ~~~~ ~Mc!pcltllw vi lie"""""' lhluld t!Y."Wy~ \It~ In lbr: _ . , fir~ ud -lndtort~lellla.

Moioi.lunbo 1998

Vni.IVN•J

3 51

REVISTA PORTUGUESA DE I'J\EUMOLOGIA

Clinical value of rapid molecular PCR Increased detection rate ofM. tuberculosis complex in non testing in TB'S diagnosis pulmonary specimens, using Gentrob R. WEJBORA, WR. PHOL. G. HACKL. F. KUMMER, PM. amplified M. tuberculosis direct test BAYER (MTD) as compared to radiomelric Wi1helmine-Jl Hospit~t), Vienllit. Anstria Bactec culture technique

Tllbcmtlodt {TD) ~s toto • "'~'*-~ probkul. 1tK 5.VI)'dd«tJoo~ra 111 paiJcd j, \U)'~ 10M fO~Jial lq • ~ ther.lpy lltdiriwriding fnfdw 1'8 ~JUI!lsion The lr.ldMilliW c:uhure mdbat lllke Mn 11n cigk ~~tub U~ !.be ampU&adoo tned»:is., ~ u poi)Witft~ c:t~U! reeetioftt. (eat. Antph), .:be llguc th&io suaion (LCX), or 11WC:fltl!Q.. mcdialrd ampli5wion (TMA.. Gent Plobe), 4!t dr::l«ti.;u Jq~IWXI m ono .~ •

.._;ti,ky-o£96 ~ 114 ~_, 86%, ·~~..ty.la~ stm.<;iti\icynf«tlun~

II.CIIII)Ir. (LIWWQat'-1 -a H.w&.;l lOW 94 %, lbal ufZiet\1 N«ban "' %. Speciflclt)• fur nil ~~~ IWli' brt-14%aa:1 100%. 1M hip di11ienJ ~11h1c fJI PCit.. ~ i' rchnod 10 me possibility otwty 4r:l«tion &lXI ilnmoitia~.t

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VB.CARVALJ-10. M. MA'I J'lll\WS. E GOV.E.L. MARCUS

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...crt Cli!.(llttd~lizi•lll di41llliomtlri; ~0111~ ll7"t:l$.

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I ln!;tinu Pa$t~l1r, Pt~ris ~ t :ltl l Saint Anrotne, 1"aris

' C l ll l Claman "CHU HOtel Utcu, Nantes. France

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352

Voi. IVN91

MaioiJunho l ??8

19• CON(iRESSO ANliAL DA SOCIE.I)ADE ElJRO~EIA DE M!COBACTERlOLOGlA (ESM-98) RESUMOSIABS'J'RACTS

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A cnse refJOrt on transmission of M. tuberculosi.f from a dog to its owner HARJU .1. MA RTI'l).,A 1• MAJUUK.KA ANTTilA' , EEVA-LJJSA RJJOKONI\N', MAIUA-I.Gl\NA K.AT\LJ\:1, MA'ITIK. VIUANEN 1, KlltS'IJ LJIJKKONEN',l'.EVA-Ll lSA HINTIKKA2 1

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\.:O'to•la\\~1~" o\dt'C"''M'J (\f'rn) It u.afb 011dlbol "lie IrWin!)' lllulm'O ~til. Mol""'tf 111.1~ UIIM's ol!'t 1L<11 pttne 11111be!:ll~ w f'lhe:r mJC!dlolo.'WIW ~~"' 111'111 '"'llle ~t lntt¢e lit b'olt.'l illfeo:mo O.U _ . . . . . !lf!lllll deC lfllt"'Jft tMa ~re P'l'*"'·

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.1. GUIGAY, F. VAYLET, T. de REVEL, f'. SAINT-BLANCAI(J), Y. HERVE, F. NATAli, R. Lo VAGUERESSE, G. NEOELLEC, P. L' HER

Y. NARBONNE ·. ML. ABALAJN ', M. SA LAON ', Cli/ ll. CIIATAIGNE ".B. PICARD ..

Hoopirol of !Jl5tmction of Amty Percy, Clanutrt. France

' Labor.uoire de Microbiologie,.CHU Morvan, Bn:sl, fi'3Jlce " Roc.be Diagnostic Systems, Ncuilly, Fruncc

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19° CONGRESSO ANUAL DA SOCIEDADE EUROPEIA DB MICOBACl'ERJOLOGIA (ESM-98) R.ESUMOSIABSTRACl'S

HLA-DR antigens in pulmonary tuber- 38 kD ISCOMS: Immune responses to culosis in Polish population the 38 kD lipoglycoprotein from

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REVISTA PORIUGlJESA UJ\ I'NI'I !MOJ.O(;IA

Postantibiotic effects of clarithromycin, Human T cells recognize MPT64 and clofazimine, rifampin, spar:floxacb• and its peptides in association with multiple amikacin against Mycobacterium avium HLA- DR molecules in human macrophages AS. MIISl'AJIA. GE KORSVOL.D, HG. WJK&RJ , SHAiiA \1, I'll' RO< 'Ill', (;G FENG. W BRITTON, F. OfTUNO. L. HORGJJN, A JEROME, N. RASTOCI' Fac11ll)' ol Mtdicine. Kuwait Uni\·~r~il). KU\\o~it. l'-uliorulln.,uwte nt I'Uhhc I t..lth >nd the Nnti ~o141 H~»p.• o.Iu, Nurway; t:l:nt. ln.
' IM1irut ~ de Guadeloupe, Podeloupo: ~Cft 19'1rft)t.KI!I, ~ eiiO der'tlflr~ .... DCierll•llr:.,.,_)'CO:IC'.ett,;fft 4l't•oiM 11!"1101. ,_. 11>e ~~~~~ .rl~ 01 eW1 dfiiGdi!Pflli.fliNIII lil'l fll';:n•~atccdu""'irJ'ItrU!ml) ~"' dol;:tiii"D !Pill""" CGUI'M 01 "'«~netn~r.:o, 11 ~ ti'te ~ o11fecton ar..d nl tfiVn~OIIilllffiQ P• ... WIIIW-1iglt.Q Ill' lhw 'P..-I"'IIt11Uiit> b!Jw:)1e !111\alll'.nflle!ll (l"•le)" llo'ld' P\olW!d f'rpoiJte j f'f)" effetiJ e/lllt IOCO.f O'IIQI .-;ll!r1t M. • _.1111'!'! Ill 11\Uif'..tn

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Recombinant 38 kDa protein and a higly purified antigen from Mycobacterium t. in Elisa serodiagnosis of tuberculosiS

Effects of high intensity/low frequency noise in the reactivity of pleural milky spots (Kumpmcier's foci) b)' mycobacterial infection

MARCHETTI D, FAVERO A, CIMMINO C. DALLEKA M

OLJVUilA M.IR. 1, I'I'IU 'IRA A~ 1,..\GltASAP 1, GRAJ\UI;. Nil.', CA~TEI.O 1\llANCII NAA 1,

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356

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Vol. IV N• J

MaioiJunho 1998

19' CONGRESSO ANUAL DA SOCIEDADE EUROPEIA DE MlCOBACTERIOLOGlA (ESM·9R) RllSUMOS/ABSTRACTS

Reduced T -cell responses to virulent M. tuberculosis in pulmonary tuberculosis patients

Extended major histocompatibility complex haplotypes in pulmonary tuberculosis

ORDWAY D 1, AKKOZ: M2, UOCKKELL H', VENTI JIIA 1>1

P. SANTOS", A. MARTINHO'. 0 . SIMOES", A. FIGUEIREDO"', MJ RASGA"', L. OLIVEIRA"', H. BREDA COJMBRA'.

Ccnlro de Mulariu c' ' lra.'i Docn~a.'i Trupicais. Li.shon, Portl@l~~Londun S<:hr." 1fHygicoc & 'I nJpical Medicine, l.ondon, IlK. 1 Hospital Egas Munizt Lisbon1 PortuJ,taJ. ' London Sehoul of Hygiene & Tropical Medicine, London, IJK. • Hospital Egns :Vlo)HiZ, Lisbou, Porrugal/F~~<:. de Cicneias Mooic
A S.EGORBE-Llils'"

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Interaction beteween mycobacterial glycolipids and human nentrophils M. RIDE:LL', J. I'AUJI'1, C. J)A JIUiREN 1, AMS. AHMEJ)2, DE. MlNNLKlN'.

1

D~o:purtmcnl or Medical ~-1icruhiolnl?.)' and Immunology, UniveNaty of OUlcl>org G<.ilcborg. S·wcdcn Deprutmellt of Cl,c•ni:;try. Uni\·cr~ity of Ncwcastlc, Newcastle upon Tyne. England

M)'(:o/xn:ttnNm u,!Jm·J,Jo.v'' •nd ioevt:ral Oilier mycob11mri• l£t intra«:ltub.r pa:I:.D~<'M i c tht')' can su!""h'C' 11.nd TI'JJJtiply within ph!gocytcs Thi1 Clpt~it>' :nis:!'11 c- g be: dt•r- 11.1 myi:ul)uCII:':I tltl \ltrf~e tnultcui('S :.wh us 1he QC.m1pkx «U waU Sl?~.:olil)ids A mic:rMilet based technique w.flll dtM!Inp""'t in (It'~' le> t;lmi.Y Ihe i.ntcraclion b-e1wccn m}"(:obaQcri..l 9Cil wall lipids 8J'Id plngoc)te,. H:un\allneu.Hntlhil.s "'"~ <~110\\-ef! to intCfiiCI wilt! purified mytobattcri.ll gl}'coUpids and 11lc production of relCti..e OX)"!C:'I radi(aJs WU roJIO'itted wtth l c:bemjNMJ~ t«hnJquc Pl-lenoll<: g:lyl'Ohpids (PUt..:.) fuJflt fo~•r mytll"'ttcrial ~C
ntutrophils., v.tlik PGLa from M.marim1m and M.btYws BCG did no«

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Malolhulho 1998

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Voi.IV N• J

357

REVISTA PORTUOUESA DE PNEUMOLOGIA

Detection by Polymerase Chain Reaction of Mycobacterium avium subspecies avium in formalin-frxed, paraffin-embedded tissues of captive exotic birds (1983-1997)

Use ofrestriction fragment length polymorphism for typing Mycobacterium avium strains isolated from animals in Slovenia OCEPEKM 1. ZAllAVNIKJ 1, PLSLAK M1, 1'0SHill J1, L:VETNIC Z'

ZOLTAN S. GYIMESI, BS., ILSE H. STALlS, DVM., JANICE MILLER. DVM .. PhD., CHARLES 0 . Tll< lEN, DVM, PhD.

1

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Veterinary f:\c ulry, ( ierhic:eva flU, 11 15 l.jubtjana. Slo\'enia Croacian Veterinary lnt'ititure, Savska 143, Z.agreh, Croatia

Rr.llrittioll lhap11cnl ltujjlh !XII) I'Il.>qllo.l•l' te»l:tJ~« (JI.f LPJ wa.s u•d

lowa Slate Univcr:,ity

to ant~·se

h-6t'tl~lii.W rrwum inmr~ \fr&m~ cw11lnr•l rmm ''ilnou~ .a.mmal!. 111 Sl:c~
A Fl·nWI~•-e d.apilso(it'ii3 &ba!ulMh 1!1& \I NJ! t.'lttn lll t \'iu W~f hi• \ I:.Lr:t...,d,RJ: ~ut)~'t,olnJi: lt lioo t>~i«:r.;?l! lt. ~t p.rpou Mlbe t!Ud;· wss (11)(1~1! wh.rM !nnr~CI.\f\1. j;1UaffU:.·UDI.'<9.1d , .L~hh·;l M~ ~ cocl.d ~ teS«J Jy IX\~ cbllb n:lldu:. ( PCR) 11: pN\'ilk ~ ~~tii:ltoe and cure ~d. 4:i~ ofa.-l.,_l'.l~u.lo,i$ TiJSite~ Q'fft I!U."'Ii:wd fin~:~ bcO pocw::~ljr.i"'t ~ 'iL""~ tltbmulotls &ten ~ve r~o:r,.Qr. \liJ.t.s awm!:t6& 14 yw: pcno4. T\e ~'" ueC fw f'Ck 1Cp1ffie.4 t l~ &;,v.'~-~Of \6$ ra!'\.:'-, a StqUeo:.:t ~p~:iflt fw t:.;IIL. U}M'>flcruf-JJ~e 12'<111111 Nbtpeciet~Y(II!IIo abiJ./)oM&a.-:1"~ .OV/11111 wb~:ltfiVOII"f.,.-~-..1~"· 111 Cct«tioll ~ I2U6r em 1 Ymplf:, il \YU pt(!IS.mltd t»l /tl Pillll'l111btp. "~'M" ""U il-.c: Of'UIJO:. llti:!a: &wo::~ed. n. PCR US.:t.\1 lhc "'~ iD 26 aC Ito¢ 97 s~kt (1~) Sow or W r..q;aciva PCR ru~ Oil)' \lc ~'IIIC'd by Illy oftt''a'll C.:tDD e,t at\ltdTm ~-nbrdJt-:1 t,~~~.~. b9<~.t''tr, b: )(!o.+ 4,cccc~ocn~ iD 1lb 'CD;IIt srtmi.)' I.W\o!IIS ~ttl\1\t u & d:lilll,r$'liC ~o»l,

All ll:co !l.!.u;" Wl!IG ~fat)~ l''ill)mtrm chain ta.(\!On (l'CI\) ttlil!y$($ t.a•·t J~OV!ft lbt l'OO V>N" a;f W<'mun !~e!qutcu:c ll-1~0 1 1n allto:t.ICI! 'U"""· CitnOil'llt DNAs ··••m ;,ol&tcd L."t:i C.._suttd ~~oitb rndonu(lcr.se Pw f! Tbt nh1~Nid 0:\.\ fntgmrnu v.r.c r "'1'11"1'11 r.n ~lln:~>e t4d. t• lnt:"ec~ lv a mcmbune &tid bybrU:ised .\5 t " ' Obe +.a.ot hnvc ~~~ llr.« tr Pf'R r rmt..n 'i'~dk fi:r th~ JSII•Jl. Kfl.P anal;,s.i 1 11.·u pe!'ferrned using CCL dd U'~'~f!lts of 0:\A ubtl\ml!d li-nm. Ill¥. 111:ull.!}, t llll*. l'lluul, c&ule a11d b.mca11. 1 he o':ll.ai:ICJ f~i11lt~ lndicatt tbalm Slo..,rnit u.e bn~e 111 !('nsr si• diif.-rl'l'll RFl.P l)'llr~ m ,\/}<'nl\Jrcolrmm .nv.Y.I!I 0JI lt)I.;IIS IIU!J~le lhl: RfLP anal!>''is is u~fJI tool (« tl:o:
u

Occurrence of bovine tuberculosis in animals and humans in the Czech isolation of Mycobacterium a:fricanum Republic in years 1969 to 1996 from a bull in Northern Bavaria I.I'AVLIK,J. llARTl " I. I'ARMOVA ', M. HAVELKOVA " , M . K UIJl N " , J. BAlAN'I "'

L. NAUMANN'. A. WEBER'. D. KAHLA1J}, ll. WOI .I'', N. lHIN'. U. REISCH!.'

Medical M1crobJology and Hyg1cnc , University uf Regensburp, l'ranz.Joscf-StruuB-AIIcc. II. ~JOS J Rcgcusburg,

• Institute fM

Germany

' LUA Norbuyen~ Heimerichstr. 3 I, 904 I9 NUmberg, Germany ~ LUA SUdboyern, Veterinlirstr. 2, 85762 Oberschleisshcim. Germany ,\~(C<.Ibi.I<'J,•~illllfl ,,'fi:r(lll/1111

i'>ll ('lllhl>£1!1'1 £1)111111 in lllbei.:'IIIU!o.!S p1t1~1~:!. 1:1CtttJI!I jl:l!li VI

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lill nhr.nnll!l'l\ ll t' 11 )'r.un~ hull nom.~ l~tmJ ul n !l!t m 1\\111 htm Ba.,;u~ tpldtl'lliole>giul in\cstitpti4ns uti~! IS6t )0 ftFlJ>·l)J'Itl$ .:kmMV.Ut\:d tha : tl:.: ~ex~: ot' int~\:til'ln \\;t!l f'tlllut+l)' II M!
V~.:lcri.u~y Rl::;I:'.C~r'~;h lnstitote, B1110. Czech Republic SUtlc V..:lcriua!)· Labot'OtOt)'. P(Qgue., Czech Republic Smte Heolth Institute. Pmgue, Czech Repltblic ... State Veterinary Administr:\tion_. Pl(lg:ue, t:1ech ltepuhlic: .\to nr IC· O..:I~.....I. 19~!. 1.~~ .;".. \llbr.~oii J>IS 1!1 ll•Q!O> W&; ,,m,if!l:<>(J In 11:.. \.:!C":!. ll.t;"'.IC .: ...11, 1!1 11:.fnlll!. ..'alk. (ll IUh OtUI clunonuo;m j:l ) iflmll'.c: ll'h\l t .) I')Cii), ~"'- pol1t 1U::CII:O:> r<'n)~ ~ 1969 ll I!I\16J "'a!.l)p.cal bf''tr.llhin@. of ~(W:«l SOUit <' fUCI"'IOirJ d'.::ril'l! ~~"t' (01.,..'"-l ~tl..~ f.'"""" 'M'I}'

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011!y t-porWlc UJC an rnocdd ll"' tc p•rlot )W1 l'l~ t(l l 'l~ t--wine -:..\erc;cle•\~ V..!.'> r.e':';ly l!clt:ttG m 36!1 !erml~f<$11'(' (" ~ 1e"t1 lllf~W>\ will' 'J;'I IO? •'ll-")'$l"'V."; ~r.d ' " lt."ttr f.ltn' ·. will\ mn~e tn:l', J0 d t'.ty ~0Wt) h'~ !IUIII'ftllt( t ·f hMII!t llt~r~II\Y.it Wft_~ ra11nd in )'UIIro IIi'! I, 1911";1 t il 199/l, 19n ..-6 l ll'l" tn Bt l:fl".lll' int !o'erlt.d hetw~n 1910 and 1~. dttlt v.ere alwl~s mMh"•.tm l nmlmonh t~r hn'tire mllln'llln\b. in wult dt!Kted II"' )•u-. Th• 1Jl11 in!et:rt! IIIIU:WI$ 1\Uit~f lht 11\QI 'li« ofbetd\ Wll'o U Tj=.JU a..-.d did COl u1;ud j 1., 10 H ul •cmn•h, In )'NU 1971110

or

.\f)\"ll;llm.,i ll.'fl t Q¥il wa, ..:W dii~nvid II\ 1~1<111.' 119 t11ur~~ l.tA...,.I\111111~ p rdrns. wild ~m11ub, '"'"II t•n ~··•) a11:t 111 JU u;npl~' of milk In the i!fi$C o! Cit ( l!efin11.X. (ll!l~,,.,;,.,.,., Anln•.al t~ca.r.ll Co«pll: ICr.ol:or;.·ohh~ Cud! Rep.,b)le b ~c lh:n be~iM tul:n~.,mu {p:cvlltr.ec up.~ 0.2 'll. of in!tcl«t herds or ~·..It )> l"'!11111111n Jll'l('Ohtll)ll !11 l""" I'J(i't 1,(1 1~'16 1hl' f(:lt.l~ o( l<.tu6e~er.·.'O$U '111\1) ~(ord(\·e 3n yenn (A !1: 1r ~ !ll l'~ ' 1M tllll I~

1be

infecli~-., ••~h

,r.:d:~o:l:taJ T",< - .-r&:n.·r tri:+tb<'ti'O )

rnocc

Vol. IVN'J

'il"

1'-1 111 , ,• ~·n: ir. nlmliur.: ne11rtl'l "'~ l :.-a 19 ~:.~.:..-. ,t. ' '

:.~ :1 It

Maio.:Junh<'l 199R

!lr:'

l9• CONGRESSO ANUAL DA SOClEDADE EUROPEIA OE MICOBACTERIOLOGlA (ESM-98) RESUMOSIABSTRAClS

Some unusual localization of animal Detection ofiS901 and flanking region in the strains of Mycobacterium avium tuberculosis complex isolated from swine in the l. PIRES, M. PIRES, P. RODRIGUES, A. ESTEVES, C. SAitAlVA, Czech Republic from 1996 to I 997 C. MARTINS

L:nivc.rsil)' oftr.i.s-os-Monte!\ e Alto l.)ouro, Vila Rcul. Purtugol \'.e :--~r"~'t ,,.. f':Nellt 'r.mt•mtmul r--un

ihh t!nut:c:n wu d~l'l:w\1 m ~.c 19\11>~•1;'1~ UIIJIU) llujl«rion, ln M•~~· ~~~~~~~ ~doll....,.., d~•IOhl:rt'lllln 111~ rtl htnal a~ltvhc. "'"' umUI'\ 11:.11 don' l ho•·t ""' !l'i~hl"'\tl&i""l !lilt M'IU/Im~!f*lt.lllns:lcal IJII;,n~l"'"" iO 1/ICU: o r '"~ll'it.oo\ M dt.oohl, flit ,,~,ei~M)' hupeQ(n urA loalllp)U of~'-'"" tu*i¥1

!iltbr Vni~(":1*.' \loltiii.V.IIr:ts. lntht ram. thu •t l'l'f~mt ""'- Ill• mlilll~ w~, vnlll!liiiOMI!y, •cill Ooo •~'mll!..ltld,lm!inf! mlur.llllliii,IQI :ms impi)uilll•.

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Tuberculosis compatible lesions (post mortem) in cattle PC R and Elisa test applied to paratuberculosis diagnosis in C. SA IV\ IV A, l. PIRES. A. ESTEVES. M. PIRES. F. SP.IXAS, early stadies of infection in cows C MAKTINS

Uni\'crsity ofTrfls-os Montese Alto Oouro, Vila Real, l'onup,al U~"-":11'"100•"' fot,.Tr IIM!tn nft:tll .~~.W.Itl'b\<11 ~a(MiJ IDS .46~......'11t~t4111""' P~~e u~ 115 etoo~-.uil. •1oJ l'\lllt.c Het ill! impoo...n«, d'IIJ di~ 1:011.~._, II) 1:1""& 011111 1"111 In~~•. b f(tmt (of ~Wiflr 't"tftn11!«.is Wlr-Jkotl.illll ~\Ifill -. ll "'lllol,!' I (~til''( QC> UIC a:lb\1(.<14011111 p!l!lt'lln, wuu.d..a) 10111 111d u>~tiMd ...iih IKMUit:~lllll nnd eotln. C. v.'l.1 a bo subj.:t 10 •Zilhl ·N~o lf*ir"

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CU(>I).no.

F. VITALE. S. REALE, I.. MAXIA, NS. GLOKJOSO, S. CARACAPI'A lstituto ZooprotiJattieo Sperirnentale della Sicilia "A. Mirri", Puh.:nno PCR (o;>~n'..(IC~ Jnl'l 011C Hl\11 b~l'fl:bUIJ(Ill ~ef-t 'OI.tt l ptrfCmlt; ~r. ~r.iH:. h~ll .Jrd bl.:>~d :.:.:n pt•.s· in 01lkr tO :rakt (3t\lttlbcr" 'l<'$iS dii.IJ."~"" ~~ m o~n.tmab ..!un.:111 ptduliCal nN;- ;)( ID!Il1:1i1111. 1HlllloiMls w•rc: C:X.LT.III.lUd in l'lrtt fUI'I'U l~ru,•,-j .n ~k!l\ Ric:~.:tlt.:ul;u lol'~l:. 111-!~>perlonrOO ond W<."fe .;.,m~"!lltll Wllh &lllfldirtct t\.JS,.), :tJt lOt;(:( tOulln.'h' ll~~'d h ~t• -ll->ii,JI J~;gMoSiJ. For PCR 1~s• sper.ifir. p fntm.e.nt. !o. Z29·b9 WIIS dt:~l£1\ill•'r. to~ ne-1.:!1! 1-'Ciot IM ') 11l.t riru 41r.pllf:e&tlol\ rt''-'rim:• amt a(tt• l:o$i~ Jatf'l c¢ "''lt emplo~~ in Do• BlrA 'h;.budu.oJliVII (UI. Scnaici~·ic:o: oltd s.enslbilby Qf 1ht ~o!llouJ!> "'1!' 1! mabh!M:1. ~ctui~'t U l':'lples in tl!,• '£liSt\ (&':\i l~vl) ll!~l.uun "'~ft !!IN ot:c::lcf!, Ot¢rr.Ol·~<:vlu r 11-r11tqo~ 11'111)- be: lol(i:ii:.ed ~ l');l (or nt!UJ~i.-,n .,. F-lr-llltbtl::ni:IM:> JlltfllOSis ..,ith eitr~ltM nl( thoch ar..:l ( ut J!lt~t:vu vi !.lttr.J forwJ ~t

I!~.OJtf'. :avoiein! 1!'1.! ;prr:1rl "' I..IIII!CioOO io C'lt f.um~.

Mo.io/Junlto Lq.QK

Vol. IV N' 3

359

R£VISTA PORTUGUESA DE PNEl:MOLOGIA

Identification and spoligotyping of Mycobacterium bovis strains isolated in Jtaly from different animal species and man

Nontuberculous mycobacteria in cystic fibrosis patients ADALAIN ML.. ~ARBONNE V., 'l HIJJJEI("J J .• SALAON MB,

Lllboratoirc de 1JactC:riolog1c. t:lll l Mnn1J~n. llrt(l, l'r:lnce Ll

FERRJTII 1, S

rAGLIADUE 1,

E. TORTOLJ'.

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1;. CAROU, N LOMBARDI. E. TORTOJ.J'. C. BAGLIO~! . 1- l.J.VRJ':, A.JIA(ii;(ANI

S:nc and

l~nnuve

Medic. lie l,;nhersity of PiliB

(Italy)

OJCK van SI HJI,IN(;t:r>. PETRA HAAS. KRISTIN KREMfR.. RFMCO van der flOCK. JAN van EMBOEN Mycohact< tnvironmcnl (RIVM), 1'.0 . Box, I, 3720 IIA, llihhove Nctllcrl•nds. (d. VWI. [email protected]) Aa 1 tQIII! 4lf tNt 011111~ ~ ONA ~~lf"'*O IWJtt of~ lftiCI'06' .OW.. IN!A

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360

Vul. IV N'1

Mau>l.lunhn IWX

199 CONGRESSO ANUAL DA SOCIEDAIJ£ EUROPEIA DE MICOBACTERlOLOGIA (ESM-98) RESUMOS/ABSTRACTS

Atypical micobactcria in environment

Mycobacteria in water and biofilms in drinking water systems

MF. THOREL•. R. MOR.r:AU..

JOSEPH 0. FALKINHAI\1, lll 1, MYRA n. WILL.IAMS 1• CHERYL D. NORTON1. ~lU<. W. LcClJ.J;:V A1.1.11 m'

' CNEVA-Aiti>rt. IH' 67, 22 rue l'1erreCurie, 947!13 Mai!
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Mycobttcteritt in tap wnter: role m human diseases V. VINCENT lilstirut Pasteur. 75015 PMis. france

SIN I SIIOMAI.AINF.N 1, Plll.IQ TOR.KK02• MERJA SUUTAR1 2, I'II.A IIVANAINI!N', l iNJHCO TOR'n.ll.l', LARS PAUUN 1• MA IUA-UmNA KATII .A'

Mycobntteria otbct(bllft wbet~ kcilll !Ire bttelY fttJeot ID 1M ett\'~:tt ~ llleir i:nfecfi(ln$ ll.llllll>'llm•l!ll ln be~l}ired froo l.hec1wiron:oent. The)' ircqoec.U)' coor.unil'l111e Ia!) willet 'o'"'lliclt may be: tbe S..'lUI.;t f<:r il)(eCt lun\.ln. f:u.r(Jt~~e, M. Jl,lmkmfJt!, .W. unnpi. aod.M. ktllullJU repR:&ect d:lc pttdomilwlt speci-es , ,\f.jortuiAJ.m aOO /If. chtiC¥10~& Mt

lut ftcquen.tJy iso.Jt((d. ~ tJSA. M. a \iJtm i1 o!t~:n ltOI1tcd (iWQ u.p ,..ater wbe"~U ~lkl\ isoladoo temniM mce ill Europe C
lnsLilutc of Biotechnology. Hn-000 I 4 Univei"!'Jty of I JeiRinkJ National Public Health Institute, fin-'/0?0 I K.uopin ' Kwpio Uuive..,ity Hospital, Fiu-70211 Kuvpio, Finland ' Bocte.riologicol and Virologicallabornwry, 50139 Fircrw:.ltaly We h'"\'cc~iiiiii:O.I "'WII:f" ~ (o<:~W r 100,1U$Ia su~ c.:>c....IUII: ;~~ ld«Wlif• ofetroUOIIO~

m~ 'NS ~ Go:Uinbolu Sllxook •Mer ''"'~''" 'l'fCK>kstxd fwll"'"t:li "'~• pi~m«tt pcodlfrtic.lnfl jU!"''(h II 111, >·:1, l f, -U .1M 41'(" _ , AIJI) IIMI.t~d b)· I;IIJ 1!qltl.4 ~.h~hy (('.IC) A~$ lei dltOv: \loa -.1 0C (!til~ w~ Rlfclllf &me .wain1 krf ~ \fWd)' (E4l, El~ I Md Ellf) ~~-w 1o bllt:lw. w.,~~(fonll•u~·~ 'l'hf t)p:~of Ill '...vp.'. AT<.'C Ji'UO' ,..~ d10111111¥tltc 1$1~.-: lli-

'lp('f!i~n .,.,.i~h

e.xogeoo~ fO)'CObaCtt(JII.. ()(10 lallO'l;tUu: g!C31 Mlf:lltlii'.L'e!.l~~tUid, ~lttl.lm:Jl.ICS., IXll'Cicoide\, mcsolher.tp)'), to ln\'oUI~'C Ci~r.G1-is by cr.do~o~y. to dia!)'s.is, to ~·ou.nd IR;l!mc;~c. <:r to ~t.:.ril:l')' ' t~:J~ ;o&:1:ic:ad~; 111. s:-trlliu~a ol' 'o\!11-!r, sol~..~. ~t~tdical. ot sutgicn.l

drt.g

r.·.-:•;

iosuumrnu. Auwclt~ve ileriUzodon bus to~ sm.mgly rt'Cc-mtntndc:d

Jikf~{O{II~rtr.ailll~mahfo· or~g»liquid~pl~·o(edlulatbfto.·~l!c~f!oiJMI\i ~'O..'IIk xidd w • ~ f'.rol W..n.&~ "Mohr. wl!lo ~nttx -trm oriltx-~o::on~l R~~ r~"' The ~Mit<- IllS rrt~.te-.•n.iW inll':flllll mtMI'lriMj ~la\et (IT$) flm','Uft 1"'-C: 111111 ~>< J111:S. t'.'l:lt ttq~ Wi.ompr~I'M tn fht: uq01mo: nfW l)-liCMmill n.t re~•ID«fftv~ lh."la.tdcd.OMofthebd~~~~:~;, eus, "ullilm1• .. 1J. Xf'I'MP'· Tolllll' pM~'J!~bm\lerl&ll!, tis is Oktir.:t fiiiP.'ituf,t/. ~r/'lfJpl &vm ~ ":Jr..tr.l lbeoi:lw:• tllo111u>llU\. E 1: .ar..d f""· " I'IY~:I!IIOl':Kh••h•"*"''' o.'•'l'":
Jt:od ,•.- ;

t•• ·•~

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(JI.\.' .....,l~s.o~ .-.;l ...ioclio.' ~~- n011 d.:IJA iAcl!:lld tlo..ol \l!<'W o•~tl:ok' b:IOilli lo)l ""'' "l~~l<:tul spedts Jld"'~PfOJIO'IG ittobcillanltdftto~<11:'Jtl'l""'~VOOIC' Sp-. IIOto'.

MtliQ/Junhn 199M

Vol.lVN' 3

361

REVISTA PORTUGUE:SA Oil PNilUMOLQ<;IA

Role ofthe integrase on the mycobacteriophage MS6 site-specific recombination event

Characterization of a mycobacteriophage Ms6 promoter region

1o:. Al'.-ES. J. M
M. GARCIA, M. PIMENTEL. C. AFONSO, J. MONIZ I'EREIRA

lab. Mk.Tobiulog,ia Molcculur! Fac. f:.mn3cia. Univ. of Portugul

Lisbon~

'-J)Nt>.O:kM~ ,\1J:> a t ltmN•~It ~~col ,\ttovt.... ~oc•;..., ..-¥-.;;. Jw lu:m• ..:.\>~ bW4o:l' !lpfd& illlt,UiiW ;t~_,!;ti>IOOI, , ,_ ll!IVJikt -;U»IIf \M \ofta Pl"'icJiidy t~:leilbl Wid ~HIUd

lo. a t h'-

Ttc ftltt!U< pll'lldll It i't\liohd (/lt lf~ I~IIN\...u i1iM c.UI> n( '"""'' ~,...,.,, ~M~>I¢!' It) lil!llllY.u4 IIII!Mr O f 1>111> ''".VOI~n·~o.(oJ..., l"tfO Mil$ G (~!lvlllllf)' atnl...u•ou.
Ill flP f11:tw'l \Itt IIIII ,. lllllllle M•f> ~ Mtt'IUI i\ ~ o:loN'Iy ""l"i:r-4 ~ ltl( ir."t.&n!!"!! c<>~lll, I 1!1! 11"10:,, .... lb•IIW uo tywwu~ lh< ,.,lwsco:~. 1'uJUbgr~.,l!td ll~r lltlb.leulw !!It tfiMI!Miw '.t"!' .:H ro !he "'''rv lo '"~tif r~P ooo• .,r 11114 oiiele.u M'lhr. ~·V. <>ttdo IIIU;UU tl,.l !110 tll
..,,..,*,. ,..

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Ccncro tlc f'utogCnc:;c Molecular, Fuc. fWTtuicia. Lisbou. Portugal A !SO bp m}.;l!b-.;._lillphllil• Ms6 pn.'COI• rqiun wu drr.«led u10inr • pwmiXw ._..,ine; u• &o·h,,if.'k,,_.,.;,U l;x.' l jjfl" ti 1'11')1\ft PI'"'

r.rnh~ \C.:wt,

I he • •m Q( lh!:J 'llld.fWU lochatac~ritc lhc llt!Ot:lc r-lco'l~" of Ooi• p!VO!I~kl ltp.lll, Th< IIUI,Cii):Cion~l ~U\ silc, . Ill WU dC1t.rmind, ollifta: the ptlllltt•CXIJ;n~.:•to .U!41~51.. &!OJ ll!f -Ill rt,!:iGfl (T.~C'ACl) '*''ll; !«llo:d lll llbOIII7 ~ liPJinam. Weo did no: find lll)' c.>O>CII$'J ' . l) to:Qn.n !IC:IjiiCf!Ce •\ luo!.ct \c.'f)llt fl:t l'irll 1'>0 bp Yor:.. fn1111d flt:!wll!'fl lilt lUt'•\t~n!"n Jl(l, Jilt tt
tw!inr frnme, ~\ ~o..Td npen n:tdin¥ ft.llt!t 'MUS mopped 2.~0 hpdnv.n:mrom. U:lf)'in& lh ~ IXlfl\lnn:eio:n did nul Mnw uny jl plntm!.W..e .1o:uvll)' 'rtll< rt:\lob cr.nr.n~1.~ .,..ilh the lliq. le\·r-1 nf Pzrlntuddme ofllhi'l)' DfrMGI, .,.'bleb dt.N nQJ I!Jwt 1ht l~.tH rot~~lll!nce. l n \l!'U1 10: IPI'f~o~iv~e 1.~ ~lrKI orllli' kodn ~>el)llt!flct in. 1M lir;e OR F tl".l'~\~bn. n n.-... ~~~~~M~U'-'n w~ ~~'"" '-"o4 Ill wi11WI ll1• ludce ~"'fUfiM "~ h.-wilt oh:lesed. \\'ish !.hh ne"'· ~~n:oo'....:dll!ll, a hi~• W~tl ;l·t:tlu:u.~~ .wi~iq ll» 1Ktli11t>ed. Thi' lllldff u ..,~er.:t M~ m.lu"'lu~mly in ~ol'ol'd inl~ :he pln,mi-:1 t. IG I and 11 d:g:lilinlll de~Me Df 11·f·''·1t.JMidMr u 1" II) '.\ •;

ur

....to.med

Identification of a putative haemolysin Mutation inpncA is u major mechanism of pyrazinamide resistance gene from Mycob(tcterium avium in Mycobacterium tuberculosis .~

HlYNI:::S.BW WRllN.SS. OAS

\·11crhh1al Pa th(,g<.~nkiry Research Group, Depattml!nt of Me.Jjcul MIC.f()hl(llngy, St. n~ rrholomcw's HospitaJ and the RoyoJ Loudon School (If Medtc1ne and n enristry, l.ondon. UK In d("'!lll...... ~,.,,.,.,., «tf••"m~ l:t!OIJ0nt; Ill U t . •\l)~lofll• " tJO ' '"' COUjllu (NAChoc tl.c "'"' ""fllql 1•/ll 1/(h*mO:bt o l~ltl~ li')l)oi-II.NAC ;. • ,. . .,...111r P' IOI>GI\IIY.nikl).'f luxn lfl• wm!M:r o t l -ll..lu polh.'f""\ r... C""'ll'" ~ut<'•il l(lo:~bw .,,;~ I o w~u -.,.,_,...,1V..,'' II "'Ill'> f'l>p~o, ••»e ' "'""<)l),.:' "t''"'lo I •r>he"'ll,Jft , '"''thl•~ •~ull:,do:o...a,~unuN..,~...,_isotd looooo..,l y,., ~~~••~ too!'t"o•i•u!. t'-'•ll'o 'fl,.,·, M•CI.:holll\l.f.\' 1-U.M.. 1991\ h e:b sellil>· "'" ~mp!-,fioll • J2 1 tr .N•~•i••ll.w:n11l•1in ~~'-" ~u: t ... • tMietll ow... ot v "'""' , ,.,., d:ttf!rUit fl'llftt!. tiUip!N a;:IIIU C(01U\d tcplll$ M'pin iilu!J) cl\.o~~~~ III~IOIOI)lll' (tnht1: U ..,,.... .:l:ww .....wt~ fn>.uooto:l\llokW'r ...J I I ~OWoooo!..ol)""6 iloc 1-1 l>k•i ..bit CO!I!Itf"''iirt l'otl,n•nm V\)(IY IIIUI~N W~ IIII:ICf......&alyur ... piUIIW 11-ll!)oli&llfM

........ .............. ~

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"ll'llf" ..,..._ ~ It r"•:tt••·o: · mJ• ., rt... t''l"'lt ;ut. •htNI• : r ;lit "..l:l'o•l)\1~

I"''"~~ ""''•'U •*'

362

•,; .,, ' "

IIIRANO K, TAKAHASHIM.KAZUMI Y.FUKAZAWA Y.

AI!EC lnstilutc ofTubcrculoRis, Jap3.n Ami-Tube.rculosis Assoc::huion, 3~ I· -24-Malsuyama, Kiyose-shi, Tnkyn 204-0022. Japan To dwao:llriut lilt w....WO d dw muwimu • ihe ~ ~IW: tn.-nd*tt to)n.rilllA'!~ (P7~1 1';:4 ·'t"'"~,.,,_, !l.~•nu(~»l• t;o <11

lwt 111 PZ.. -.;lil·.-)· 111111 dn•lopll>flll uf pyr.am~~~mide milllUIU. Wt

dci(IIIIIP~ ~he I.Uoc:icioe of f'Z- ..:til.'It)'. 11Uniuw1D Jahi~ CUt.:tooli.oi!Oid .MI(:.). a.od oooullol/1111 1D \bt:

.fl'"""' ''

"""'~ 1\(lo'hfft! ),. ...:.~~y "-';"' ~u. Ooc: ~a.n.··6•~ oulof t 6f uolato:• ..,.ere P~ pMiUl., wl ll """' MfKil& Tht M!Csd PlA ¥ ~ fiO v.t=e nvw 41lllp&'ml ~ •II 1 )

I''•-A

f'laH'IIfllflh II ~lito ' ...U. liMo• uf pz-.pll~ilin bllbifs l!,'f~ eqllli 10 ,_I~ llwl Wi JJJt-'enl '"-2 J'J t'J!a:i<:'ll~o l't UOIUtS wquto.Otd. 'Jllllnlllwld_lololl_ " '""" !Ov poa:.4 , _, A mulalillll ,. .. ..,11 111 •.nou' ~P'M lhrcO~fl 1P1f. It -.stu~ IWI Nl dlf«. At~W OC 111 vouo 'riecletl JI+A, ~-~~~ m~w.nn vottt: I'~ aM 1MwtA 110 ~ '" 1hc 1"''.4 I f " Thuo mub lfdc.la, diM MdilifllW ~ ff0.1 lw iii'I'Oh'tli in r1.A ~ ••IJIIIM.\ IWft nbu,..M l11 ..n n 1 r z.m. ~-..tol"-c .\J .'.>'OI\rd in lht lm' ,i :•,!.,< l:• ·~lt' 11oa, n.·,,.'u M
doe,.....,.

w.,

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a.:a.: 10• .;,( ,..o,....,..,o,.,,

Vol. IV N•J

Maioilunho l998

19' CONGilESSO ANUAL DA SOC!£DADE EUROPEIA Dll MICOBACTI!RJOLOGIA (ESM-98) RESUMOSIABSTKACI'S

Investigating mycobacteriophage DS6A infection 1

~AIUSH.S.

HOYLE. R. McNt lt.'"I'Y. NG. STOKER

Lonfra(lomt n~,; ttJ At. 6vv11 (J(;<;. \lu.C don IIO'IIftt«t M Ji!lttfMf'l,f I" ~lfMr t.11U !nbf) lbc; Jt llai rt'f'IIMibkl &n tUillb n"" at...cq~tibilicy- DSM illtctloo w.e t\1 \'C\ISI:(ll r.(Kinitl hbr.uy ofM ll'l'~trtwf~Jfl Hl1Rv tn~mlilmvd uu At ''""'~' -r .,.o «l~UJ~i6
If-- _.._.,....,.....,.,.kiNilolaAttalllmcr--V tc.n..l\1t) .u. ,,.,.f"t"JfJ.

,....,.....,..,.J4.~r i EIOtiHI-..ndat..__..toakd-.iN ~'PC

,..,,,_.,u.

Identification of u mycobacteriophage Ms6 DNA gene that confers inhibition to superinfection

Vlrila''t~~-w.~,....rtla...-.. .~wcn~ tG .SCa "ict

f), . At •--~ ......,.. . . . Chetot h . . . . . . ~ fllpb::g ·~ tt~Jw:ta ~- ...... .,.,......... ,'INI!b pcoducc: WlldUaf .~f'l pl••~~t !OJ~"""" ccmpla dlanto • tl'le mftnn. C)ck 1 11 1'!'hl\111l ~o~nJo'Ht.ll:di•1i (!( th.; ~"'"'"' or

t"'-''1: lnf'.:c.ru:r ll!ll~ f.u::ll11.11e 11:.:

('On"n;c.t!Ofl of $111bll! mllt.1nt.'l v.11.h llaifuwd tlltoCqltibthC) to,. . . inkctlcn. T'htte In tnm 11'1.&.) pfOYi4e \IICM tools for. dl~ Mill fYrll"' lll'-"s

l\1. PIMENTEL. M GAM! :tA, J. MONIZ PEREIRA

CciJlro de Patogcnc.S
l.~
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flit ...1I115111"Ut of

t-elttliiOI~ IV'f"'llo 111/ll~ fl. &l'. ttii Wih• \11,1 U,. L(i)I)\IC4U.J!I!e~e JVI'It l II"'

1n1pcr1-. t«~>b ~ h tfi)'A'Ihacwri•l.-n•w. "•"••~A~IMW.:.O..

Determination of an internal origin of replication in a mycobacterial linear Construtioo of (nycobacteriophuge plasmid: pCLP from M . celatum D29-derived vectors for genetic manipulation of mycobacteria C. Le iJANTEC, M. P1CARJ)11AII, V. VINCENT

l.aburut.oire de RetCrenees de~ MycobactCrics, lnsthut Pasteur. Pans

GRA~A RJUF.rRO, TER£SA COSTA. JV COSTA

I lnlt nf Molccul~~r Biulogy, Mcdtctnc, Lisbon, PurtU!l"l

~utitule

1nr llygicnc and Tmpi""l

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Maiollunho IQ
Vnl IV N'3

363

REVISfA POR'JUGUESA DE PNEUMOL001A

DNA site-specific integration into BCG chromossomc by transcomplementation 1\. VIF.IKA,.I. MON IL I'I!IU'.IRA

Centro del'arngcncsc Muk;:ulur, fuculdode de Fruu•aciu W. Uuiversld•d• de l.i•boa. PortuMill &'f), .., .,..,...._ 111'1111• of M llovu. ltntklprd b) Clllltt'.- w.d C"llloir11, oll...n ..;:,... ~ MUfU .U tclh>c, .ctkk tJi bdp -~ t. :«ttll )fin JttoCdC f00l1 b ~wha.\M.. fiii.Wt 4ndOf"4 ·~ ck- Clqlf" ' * of ......,... fHQCC'I.\tU -._ttCit Of llfltormt ~ • IK..U. Ho.n•. ,..,.._. ''11!1:11:0 - _ . ,."7 !tol;lbk 1111 .tt.cr..c nl • ~ tdcnlite Ta~ .__..W)"('(dw ~ ~-..,,~tc• • n\:A ~jljlgO ptouu kMd oe lt:lt ~6: ~ ·~....w. ol UJ:Iflt#dc:

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Voi. IV N' ~