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1P-0055 Lipoprotein phenotype and adhesion molecules correlate with diurnal triglyceride profiles in patients with coronary artery disease
Monday September 29, 2003: Poster Session Coronary artery disease and LDL-cholesterol) and neopterin, neopterin was the only predictor for combined end-point of death, reinfarction, and readmission (p=0.042). Conclusion: In acute coronary syndrome, serum neopterin is a predictor of severity of artery stenosis and a prognostic factor in short-term follow up. 1P-0053
Matrix metalloproteinase expression in patients with coronary artery disease
T.-C. Wu, H.-B. Leu, C.-P. Lin, W.-T. Lin, J.-W. Chen. Veteran General Hospital Taipei, Taiwan
CAD Syndrome X Control P(CADvsC) P(Sxvs C)
MMP 2
MMP 3
MMP 9
MMP 2 (active)
MMP 9 (active)
896.5±149.2 830.8±123.7 803.7±124.4 0.012 0.528
136.4±50.8 118.0±30.6 92.7±30.0 0.000 0.013
21.9±32 11.4±21.3 5.38±9.28 0.041 0.297
368.4±86.6 379.8±68.1 400.97±82.1 0.158 0.567
21.9±10.3 26.7±20.3 23.13±4.61 0.680 0.509
Conclusions: The amount of total form of MMP 2,3,9 significantly increase in patients with CAD in comparison with control group. It suggests MMPs might play a role in the clinical atherosclerosis.
A. Schmidt-Trucksäss, M.W. Baumstark, C. Daub, S. Espenschied, D. Grathwohl, A. Berg. Freiburg University Hospital, Center for Internal Medicine, Department of Rehabilitative and Preventative Sports Medicine, Germany Purpose: To assess the relationship of different diurnal triglyceride (TG) profiles (p) with the atherogenecity of the lipoprotein phenotype and adhesion molecule concentrations in patients with coronary artery disease (CAD). Methods: Repeated measurements of the fasting TG and the TGp were taken in 29 CAD patients. Fasting cholesterol levels (total-C, VLDL, LDL, HDL and small dense LDL) and soluble cell adhesion molecules (sCAM) (ICAM-1 and E-selectin) were measured once. Three different TGps were defined: fasting and all other TG levels under 200 mg/dl (LL; N = 7), fasting TG level under 200 mg/dl and maximum TG levels above 200 mg/dl (LH; N = 13) and both fasting and maximum TG levels above 200 mg/dl (HH; N =9). We then analyzed the association between the TGp types and the lipoprotein phenotype and CAMs, respectively. Results: Fasting TG did not significantly differ between LL (137.0±60.7 mg/dl) and LH (147.0±49.9 mg/dl) and was markedly higher in HH (225.1±76.2 mg/dl). However, LL had significantly lower values than LH and HH in VLDL (11.2±5.8, 18.8±9.4, 28.1±8.8 mg/dl), LDL-5 (11.6±3.3, 16.4±4.5, 22.1±7.9 mg/dl) and LDL-6 (12.0±3.2, 17.0±5.7, 25.7±9.6 mg/dl), sICAM-1 (209.4±30.3, 267.5±60.6, 273.4±59.1 ng/dl) and sE-selectin (25.1±17.6, 35.5±11.5, 48.5±20.2 ng/dl). Conclusion: Despite the not significantly different fasting TG levels between LL and LH, LH showed a more atherogenic lipoprotein phenotype and higher concentrations of adhesion molecules than LL. TGp measurement seems suitable for identifying CAD patients with a more unfavorable diurnal TG and lipoprotein metabolism. 1P-0056
1P-0054
Characterization of the human homologue to the Ath1 atherosclerosis susceptibility locus in mice
M. Ria 1 , P. Eriksson 1 , K. Forsman-Semb 2 , P.G. Olsson 2 , A. Hamsten 1 , J. Lagercrantz 1 . 1 Atherosclerosis Research Unit, King Gustaf V Research Institute, Karolinska Institute, Karolinska Hospital; 2 Astra Zeneca R&D Molndal, Sweden Objective: Certain inbred strains of mice develop atherosclerotic lesions in response to diets high in cholesterol and fat. A locus contributing to atherosclerosis susceptibility (Ath1) has been mapped in mice. Using a high resolution mapping cross the locus was narrowed down to a small region of 0.15 cM on chromosome 1 (Phelan et al. Mamm. Genome 2002; 13(10):548-53). The present project aimed of characterizing the human homologue to the Ath1 locus. Methods: Shotgun sequencing and bioinformatic tools were used to identify the human homologous region to mouse Ath1. Genotyping and association studies were performed in a well-characterized sample of 450 survivors of premature myocardial infarction and age- and sex-matched controls. Results: The mouse region was characterized by construction of a bacterial artificial chromosome (BAC) contig, and sequencing of a minimal set of overlapping BACs. The corresponding human sequences were the objects of human association studies where SNPs evenly distributed throughout the entire region were analyzed in cases and controls. A more extensive search for polymorphisms was conducted around one SNP showing difference in allele frequency between patients and controls. Suggestive associations were observed and studies are ongoing to analyze the expression of candidate genes present in the region. Conclusions: The human homologue to the Ath1 region in the mouse has been investigated and about 10 genes in the human counterpart have been identified. Data from genotyping of 14 SNPs covering the entire region reveal a clear association between this locus and susceptibility to atherosclerosis giving indications for the presence of novel candidate genes.
Lipoprotein phenotype and adhesion molecules correlate with diurnal triglyceride profiles in patients with coronary artery disease
Effect of immunoglobulin E in-hospital period in patients with acute myocardial infarction
M. Yazýcý, A. Tokaç, A. Düzenli, B.B. Altunkeser, H. Gök. Selcuk University, School of Medicine, Turkey Objective: Elevated Immunoglobulin E (Ig E) levels have been suggested to prevent patient with acute myocardial infarction (AMI) from the complications. The aim of this study was to investigate the effect of Ig E on complications in patient with acute myocardial infarction. Methods: Patient suffered from AMI and having above 200IU/ml Ig E level were included in the study as a group 1. Patient suffered from AMI and having below 200IU/ml Ig E level were included in the study as a group 2. All patients were followed up in coronary care unit 4-8 days depend on their clinical status. In the follow up period, all patients were monitorized continuously electrocardiographycally and clinically; also, blood pressures were monitorized. Obtained results are presented in table. Result: Congestive heart failure Death Severe ventricular arrythmias Post MI angina Resque PTCA MACE
Group 1 n:20
Group 1 n:20
p
5 (25%) 0 (0%) 0 (0%) 0 (0%) 1 (5%) 6 (30%)
26 (42%) 4 (6.5%) 8 (13%) 21 (34%) 5 (8%) 39 (63%)
NS NS 0.05 0.05 NS 0.05
MACE: major adverse coronary event, NS: not significant Conclusion: AMI complications are rare seen in patients having high level of Ig E. 1P-0057
Polymorphisms C(-1562)T in the promoter of the matrix metalloproteinase-9 (MMP-9), C677T of methylenetetrahydrofolate reductase (MTHFR), G298T of endothelial nitric oxide synthase (ENOS), G455A of B-fibrinogen (B Fb) genes and coronary arteriosclerosis
I. Goracy, J. Goracy, M. Brykczynski, M. Naruszewicz, A. Ciechanowicz. Pomeranian Medical University, Szczecin, Poland Objective: The arteriosclerosis of coronary arteries is a main cause of schaemic heart disease (IHD). The functional polymorphism of C(-1562)T XIIIth International Symposium on Atherosclerosis, September 28–October 2, 2003, Kyoto, Japan
MONDAY
Objectives: Matrix metalloproteinases(MMPs) play an important role in cardiovascular remodeling. We examined the expression of MMP 2,3,9 in patients with coronary artery disease(CAD). Methods: Total 115 people were included and all patients received blood sampling for soluble MMP 2,3,9. They divided into normal control (n=16), syndrome X (n=15) and CAD (n=84) groups. The CAD and syndrome X patients were diagnosted by treadmill exercise test and coronary angiography. The soluble total form of MMP 2,3,9 and active form of MMP 2.3.9 were performed by ELISA methods. Results:
1P-0055
31
Matrix metalloproteinase expression in patients with coronary artery disease
T.-C. Wu, H.-B. Leu, C.-P. Lin, W.-T. Lin, J.-W. Chen. Veteran General Hospital Taipei, Taiwan
CAD Syndrome X Control P(CADvsC) P(Sxvs C)
MMP 2
MMP 3
MMP 9
MMP 2 (active)
MMP 9 (active)
896.5±149.2 830.8±123.7 803.7±124.4 0.012 0.528
136.4±50.8 118.0±30.6 92.7±30.0 0.000 0.013
21.9±32 11.4±21.3 5.38±9.28 0.041 0.297
368.4±86.6 379.8±68.1 400.97±82.1 0.158 0.567
21.9±10.3 26.7±20.3 23.13±4.61 0.680 0.509
Conclusions: The amount of total form of MMP 2,3,9 significantly increase in patients with CAD in comparison with control group. It suggests MMPs might play a role in the clinical atherosclerosis.
A. Schmidt-Trucksäss, M.W. Baumstark, C. Daub, S. Espenschied, D. Grathwohl, A. Berg. Freiburg University Hospital, Center for Internal Medicine, Department of Rehabilitative and Preventative Sports Medicine, Germany Purpose: To assess the relationship of different diurnal triglyceride (TG) profiles (p) with the atherogenecity of the lipoprotein phenotype and adhesion molecule concentrations in patients with coronary artery disease (CAD). Methods: Repeated measurements of the fasting TG and the TGp were taken in 29 CAD patients. Fasting cholesterol levels (total-C, VLDL, LDL, HDL and small dense LDL) and soluble cell adhesion molecules (sCAM) (ICAM-1 and E-selectin) were measured once. Three different TGps were defined: fasting and all other TG levels under 200 mg/dl (LL; N = 7), fasting TG level under 200 mg/dl and maximum TG levels above 200 mg/dl (LH; N = 13) and both fasting and maximum TG levels above 200 mg/dl (HH; N =9). We then analyzed the association between the TGp types and the lipoprotein phenotype and CAMs, respectively. Results: Fasting TG did not significantly differ between LL (137.0±60.7 mg/dl) and LH (147.0±49.9 mg/dl) and was markedly higher in HH (225.1±76.2 mg/dl). However, LL had significantly lower values than LH and HH in VLDL (11.2±5.8, 18.8±9.4, 28.1±8.8 mg/dl), LDL-5 (11.6±3.3, 16.4±4.5, 22.1±7.9 mg/dl) and LDL-6 (12.0±3.2, 17.0±5.7, 25.7±9.6 mg/dl), sICAM-1 (209.4±30.3, 267.5±60.6, 273.4±59.1 ng/dl) and sE-selectin (25.1±17.6, 35.5±11.5, 48.5±20.2 ng/dl). Conclusion: Despite the not significantly different fasting TG levels between LL and LH, LH showed a more atherogenic lipoprotein phenotype and higher concentrations of adhesion molecules than LL. TGp measurement seems suitable for identifying CAD patients with a more unfavorable diurnal TG and lipoprotein metabolism. 1P-0056
1P-0054
Characterization of the human homologue to the Ath1 atherosclerosis susceptibility locus in mice
M. Ria 1 , P. Eriksson 1 , K. Forsman-Semb 2 , P.G. Olsson 2 , A. Hamsten 1 , J. Lagercrantz 1 . 1 Atherosclerosis Research Unit, King Gustaf V Research Institute, Karolinska Institute, Karolinska Hospital; 2 Astra Zeneca R&D Molndal, Sweden Objective: Certain inbred strains of mice develop atherosclerotic lesions in response to diets high in cholesterol and fat. A locus contributing to atherosclerosis susceptibility (Ath1) has been mapped in mice. Using a high resolution mapping cross the locus was narrowed down to a small region of 0.15 cM on chromosome 1 (Phelan et al. Mamm. Genome 2002; 13(10):548-53). The present project aimed of characterizing the human homologue to the Ath1 locus. Methods: Shotgun sequencing and bioinformatic tools were used to identify the human homologous region to mouse Ath1. Genotyping and association studies were performed in a well-characterized sample of 450 survivors of premature myocardial infarction and age- and sex-matched controls. Results: The mouse region was characterized by construction of a bacterial artificial chromosome (BAC) contig, and sequencing of a minimal set of overlapping BACs. The corresponding human sequences were the objects of human association studies where SNPs evenly distributed throughout the entire region were analyzed in cases and controls. A more extensive search for polymorphisms was conducted around one SNP showing difference in allele frequency between patients and controls. Suggestive associations were observed and studies are ongoing to analyze the expression of candidate genes present in the region. Conclusions: The human homologue to the Ath1 region in the mouse has been investigated and about 10 genes in the human counterpart have been identified. Data from genotyping of 14 SNPs covering the entire region reveal a clear association between this locus and susceptibility to atherosclerosis giving indications for the presence of novel candidate genes.
Lipoprotein phenotype and adhesion molecules correlate with diurnal triglyceride profiles in patients with coronary artery disease
Effect of immunoglobulin E in-hospital period in patients with acute myocardial infarction
M. Yazýcý, A. Tokaç, A. Düzenli, B.B. Altunkeser, H. Gök. Selcuk University, School of Medicine, Turkey Objective: Elevated Immunoglobulin E (Ig E) levels have been suggested to prevent patient with acute myocardial infarction (AMI) from the complications. The aim of this study was to investigate the effect of Ig E on complications in patient with acute myocardial infarction. Methods: Patient suffered from AMI and having above 200IU/ml Ig E level were included in the study as a group 1. Patient suffered from AMI and having below 200IU/ml Ig E level were included in the study as a group 2. All patients were followed up in coronary care unit 4-8 days depend on their clinical status. In the follow up period, all patients were monitorized continuously electrocardiographycally and clinically; also, blood pressures were monitorized. Obtained results are presented in table. Result: Congestive heart failure Death Severe ventricular arrythmias Post MI angina Resque PTCA MACE
Group 1 n:20
Group 1 n:20
p
5 (25%) 0 (0%) 0 (0%) 0 (0%) 1 (5%) 6 (30%)
26 (42%) 4 (6.5%) 8 (13%) 21 (34%) 5 (8%) 39 (63%)
NS NS 0.05 0.05 NS 0.05
MACE: major adverse coronary event, NS: not significant Conclusion: AMI complications are rare seen in patients having high level of Ig E. 1P-0057
Polymorphisms C(-1562)T in the promoter of the matrix metalloproteinase-9 (MMP-9), C677T of methylenetetrahydrofolate reductase (MTHFR), G298T of endothelial nitric oxide synthase (ENOS), G455A of B-fibrinogen (B Fb) genes and coronary arteriosclerosis
I. Goracy, J. Goracy, M. Brykczynski, M. Naruszewicz, A. Ciechanowicz. Pomeranian Medical University, Szczecin, Poland Objective: The arteriosclerosis of coronary arteries is a main cause of schaemic heart disease (IHD). The functional polymorphism of C(-1562)T XIIIth International Symposium on Atherosclerosis, September 28–October 2, 2003, Kyoto, Japan
MONDAY
Objectives: Matrix metalloproteinases(MMPs) play an important role in cardiovascular remodeling. We examined the expression of MMP 2,3,9 in patients with coronary artery disease(CAD). Methods: Total 115 people were included and all patients received blood sampling for soluble MMP 2,3,9. They divided into normal control (n=16), syndrome X (n=15) and CAD (n=84) groups. The CAD and syndrome X patients were diagnosted by treadmill exercise test and coronary angiography. The soluble total form of MMP 2,3,9 and active form of MMP 2.3.9 were performed by ELISA methods. Results:
1P-0055
31