- Email: [email protected]
2075085 Role Of Age In The Negative Chronotropic Effect Of Rat Heart Exposed To Ultrasonic Pulses
Abstracts
S55
(0.5% duty factor). The data acquisition timing in the sham groups was the same as that in the experimental groups. Cardiac conditions were evaluated before (at baseline) and at 3 and 15 min after ultrasonic exposure ceased (3 and 15 min results normalized to each rat’s baseline values). Results: Baseline heart rates (Table) were significantly greater in the younger rat groups (US1 & CTR1) compared to the older rat groups (US2 & CTR2). CTR1: at 3 and 15 min, respectively, the heart rate decreased 2% and 5%. CTR2: at 3 and 15 min, respectively, the heart rate increased
Results: CEUS showed that the tumor blood perfusion almost vanished in all animals after treatment. The contrast peak intensity of tumor dropped 84.8% in the MEUS+PEA treated tumors when compared to 46.3% (p＜0.05) in the PEA treated tumors 24 hours after treatment. The tumor necrosis rate of the combination treatment was 97.50%, much higher than that of the MEUS (66.2%) and the PEA (81.0%) treated tumors. Conclusions: PEA combining MEUS disruption of tumor circulation can induce a much more complete tumor necrosis.
The tumor CEUS PI and AUC Values Before and 24 Hours After Treatment Before Treatment
After Treatment
24h After Treatment
groups
N
PI (%)
AUC (%s)
PI (%)
AUC (%s)
PI (%)
AUC (%s)
MEUS PEA MEUS+PEA
8 10 10
37.167.9 38.569.3 38.164.7
1941.56587.6 1709.16669.8 1721.66462.6
12.663.2y － 9.165.8y
396.46110.3y － 9.165.8y
25.766.2*yz 20.769.2*y 5.862.8y
1098.16190.4*yz 811.56522.7*y 168.86133.5y
Note. —Data are the means 6 standard deviations. *p , 0.05 indicates a significant difference compared with the MEUS+PEA group. y p , 0.05 indicates a significant difference compared with the value before treatment. z p , 0.05 indicates a significant difference compared with the value right after treatment.
2075085 Role Of Age In The Negative Chronotropic Effect Of Rat Heart Exposed To Ultrasonic Pulses Olivia Coiado,1 William O’Brien1 1Bioacoustics Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, United States Objectives: Women exhibit a delay in the onset of vascular disease compared to men. The temporal link between menopause and the rise in vascular events suggests that ovarian
Parameter Heart Rate (bpm)
5% and 8%. US1: at 3 and 15 min, respectively, the heart rate decreased 30% and 17%. US2: at 3 and 15 min, respectively, no heart rate change was observed. Conclusions: Age differences appear to play a significant role in the heart rate in female rats. Notable are 1) the 29% baseline heart rate differences between younger and older rats and 2) the marked experimental groups’ (US1 & US2) heart rate differences at 3 and 15 min between younger and older rats. This work was supported by NIH R37EB002641.
US1 (n55)
CTR1 (n55)
US2 (n55)
CTR2 (n55)
Baseline (Mean 6 SEM)
Baseline (Mean 6 SEM)
Baseline (Mean 6 SEM)
Baseline (Mean 6 SEM)
306 6 17.9
318 6 4.46
241 6 11.2
224 6 11.8
hormones may be important in reducing the risk of vascular disease in women. The aim of this animal-based study is to evaluate whether age differences in female rats play a role in the negative chronotropic effect (heart rate decrease). Methods: The transthoracic ultrasonic exposure experiments were divided into four female F344 rat groups (n55 ea): ten 3-mo-old 150-200-g rats divided into an experimental (US1) and a sham control (CTR1) groups, and ten 24mo-old 250-300-g rats divided into an experimental (US2) and a sham control (CTR2) groups. The ultrasound protocol was the same for both experimental groups: 3.5-MHz pulses of 2.0-MPa peak rarefactional pressure amplitude (equivalent to spatial peak intensities of 133 W/cm2) and variable pulse repetition frequencies (6, 5, 4, 5, 6 Hz), 10-s each sequence
2050797 The Effect Of A Simulation Training Package on Skill Acquisition for Duplex Arterial Stenosis Detection Pasha Normahani,1 Usman Jaffer,1 Prashant Singh,1 Mohammed Aslam,1 Nigel Standfield1 1Vascular Surgery, Imperial College London, London, United Kingdom Objectives: In vascular surgery duplex ultrasonography is a valuable diagnostic tool in patients with peripheral vascular disease and there is increasing demand for vascular surgeons to be able to perform duplex scanning. This study evaluates the role of a novel simulation-training package on vascular ultrasound skill acquisition.
S55
(0.5% duty factor). The data acquisition timing in the sham groups was the same as that in the experimental groups. Cardiac conditions were evaluated before (at baseline) and at 3 and 15 min after ultrasonic exposure ceased (3 and 15 min results normalized to each rat’s baseline values). Results: Baseline heart rates (Table) were significantly greater in the younger rat groups (US1 & CTR1) compared to the older rat groups (US2 & CTR2). CTR1: at 3 and 15 min, respectively, the heart rate decreased 2% and 5%. CTR2: at 3 and 15 min, respectively, the heart rate increased
Results: CEUS showed that the tumor blood perfusion almost vanished in all animals after treatment. The contrast peak intensity of tumor dropped 84.8% in the MEUS+PEA treated tumors when compared to 46.3% (p＜0.05) in the PEA treated tumors 24 hours after treatment. The tumor necrosis rate of the combination treatment was 97.50%, much higher than that of the MEUS (66.2%) and the PEA (81.0%) treated tumors. Conclusions: PEA combining MEUS disruption of tumor circulation can induce a much more complete tumor necrosis.
The tumor CEUS PI and AUC Values Before and 24 Hours After Treatment Before Treatment
After Treatment
24h After Treatment
groups
N
PI (%)
AUC (%s)
PI (%)
AUC (%s)
PI (%)
AUC (%s)
MEUS PEA MEUS+PEA
8 10 10
37.167.9 38.569.3 38.164.7
1941.56587.6 1709.16669.8 1721.66462.6
12.663.2y － 9.165.8y
396.46110.3y － 9.165.8y
25.766.2*yz 20.769.2*y 5.862.8y
1098.16190.4*yz 811.56522.7*y 168.86133.5y
Note. —Data are the means 6 standard deviations. *p , 0.05 indicates a significant difference compared with the MEUS+PEA group. y p , 0.05 indicates a significant difference compared with the value before treatment. z p , 0.05 indicates a significant difference compared with the value right after treatment.
2075085 Role Of Age In The Negative Chronotropic Effect Of Rat Heart Exposed To Ultrasonic Pulses Olivia Coiado,1 William O’Brien1 1Bioacoustics Research Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, United States Objectives: Women exhibit a delay in the onset of vascular disease compared to men. The temporal link between menopause and the rise in vascular events suggests that ovarian
Parameter Heart Rate (bpm)
5% and 8%. US1: at 3 and 15 min, respectively, the heart rate decreased 30% and 17%. US2: at 3 and 15 min, respectively, no heart rate change was observed. Conclusions: Age differences appear to play a significant role in the heart rate in female rats. Notable are 1) the 29% baseline heart rate differences between younger and older rats and 2) the marked experimental groups’ (US1 & US2) heart rate differences at 3 and 15 min between younger and older rats. This work was supported by NIH R37EB002641.
US1 (n55)
CTR1 (n55)
US2 (n55)
CTR2 (n55)
Baseline (Mean 6 SEM)
Baseline (Mean 6 SEM)
Baseline (Mean 6 SEM)
Baseline (Mean 6 SEM)
306 6 17.9
318 6 4.46
241 6 11.2
224 6 11.8
hormones may be important in reducing the risk of vascular disease in women. The aim of this animal-based study is to evaluate whether age differences in female rats play a role in the negative chronotropic effect (heart rate decrease). Methods: The transthoracic ultrasonic exposure experiments were divided into four female F344 rat groups (n55 ea): ten 3-mo-old 150-200-g rats divided into an experimental (US1) and a sham control (CTR1) groups, and ten 24mo-old 250-300-g rats divided into an experimental (US2) and a sham control (CTR2) groups. The ultrasound protocol was the same for both experimental groups: 3.5-MHz pulses of 2.0-MPa peak rarefactional pressure amplitude (equivalent to spatial peak intensities of 133 W/cm2) and variable pulse repetition frequencies (6, 5, 4, 5, 6 Hz), 10-s each sequence
2050797 The Effect Of A Simulation Training Package on Skill Acquisition for Duplex Arterial Stenosis Detection Pasha Normahani,1 Usman Jaffer,1 Prashant Singh,1 Mohammed Aslam,1 Nigel Standfield1 1Vascular Surgery, Imperial College London, London, United Kingdom Objectives: In vascular surgery duplex ultrasonography is a valuable diagnostic tool in patients with peripheral vascular disease and there is increasing demand for vascular surgeons to be able to perform duplex scanning. This study evaluates the role of a novel simulation-training package on vascular ultrasound skill acquisition.