- Email: [email protected]
2P-0379 Homocysteine induces osteoblast differentiation of fibroblasts in vitro
122
Tuesday September 30, 2003: Poster Session Homocysteines and other risk factors
HDL-Cholesterol (HDL-C), LDL-Cholesterol (LDL-C) and folic acid before and after administration folic acid (Filicine) 5 mg/d. The patients had well nephritic and hepatic function and had not neoplasia or megaloblastic anemia and diabetes mellitus. There were no complications from folic acid administration. Compared the effects before (b) versus after (a) treatment. All results were analyzed in the same laboratory. Results: Hcy 15.9±6.1 (b) -11.0±2.8 (a) µmol/l (p=0.01), Folic 7.9±5.8 (b) -19.3±3.0 (a) ng/ml (p=0.095), TC 225±39 (b) -229±27 (a) mg/dl (p=0.30), TG 153±82 (b) -127±42 (a) mg/dl (p=0.002), HDL 57.3±12.8 (b) -60.1±15.3 (b) mg/dl (p=,000), LDL 137±31 (b) -141±25 (a) mg/dl (p=0.53). Statistical analysis was performed using a SPSS 11.0. Conclusion: The study shows that in patients with hyperhomocysteinemia the administration 5 mg/d folic acid for 4 weeks a) decreased the homocysteine levels statistically significant, b) the TG levels decreased statistically significant, and c) the HDL cholesterol levels increased statistically significant. 2P-0378
Plasma homocysteine in an Australian population sample
P. Roach 1 , N. Naumovski 1 , B. Blades 1 , P. Lewis 2 . 1 University of Newcastle; 2 Central Coast Health Public Health Unit, Australia Objectives: To determine the plasma homocysteine concentrations in a sample of the New South Wales Central Coast population and relate them to their red cell folate and plasma vitamin B12 levels. Methods: Plasma samples were collected from 387 subjects (213 females; 174 males), with a mean age of 54.6 ± 15.5 years (mean ± sd) (females 54.2 ± 15.3; males 55.2 ± 15.8). Homocysteine was measured by HPLC. Red cell folate and vitamin B12 were measured by immunoassay. Results: The mean homocysteine concentrations were 10.3 ± 5.2 µM for the whole population sample, 10.0 ± 5.7 µM for the females and 10.6 ± 4.5 µM for the males. Notably, 14% of subjects had plasma homocysteine concentrations higher than 14 µM and 40% had concentrations higher than 10 µM. There was a positive correlation between the homocysteine concentration and age (r = 0.160; p = 0.002), a negative correlation between the homocysteine concentration and red cell folate (r = -0.247; p<0.001) and a negative correlation between the homocysteine concentration and plasma vitamin B12 levels (r = -0.230; p<0.001). Conclusions: This sample of 387 subjects had plasma homocysteine concentrations similar to other population samples with 40% having levels above the recommended 10 µM threshold. Increasing age, decreased red cell folate and decreased plasma vitamin B12 appeared to be factors contributing to increased plasma homocysteine concentrations in this population sample. 2P-0379
Homocysteine induces osteoblast differentiation of fibroblasts in vitro
Y. Kusumi, S. Sueyoshi, M. Niihashi, M. Mitsumata. Department of Pathology, Nihon University School of Medicine, Tokyo, Japan Objective: Recent evidence demonstrates that hyperhomocysteinaemia is an independent risk factor for cardiovascular diseases. Because atherogenic disorders such as diabetes and uremia, frequently showing calcified arterial lesions, also tend to have increased plasma homocysteine (Hcy) levels, we hypotheized that Hcy induces osteoblast differentiation of some vascular cells including fibroblasts. Methods: Human fibroblasts were cultured, and after subconfluent they were exposed to various concetrations of Hcy (2 mM to 8 mM) in medium. Their morphological and biochemical changes were followed for 28 days. Results: By 14 days after homocysteine at 4 mM or 6 mM, cells formed typical nodules that have similar morphology as mineralized nodules of smooth muscle cells reported by others. By histochemical staining and chemical measurement it was shown that homocysteine induces increased alkaline phosphatase activity compared with control. von Kossa staining showed calcium depositions predominantly in these nodules of cultured cells under Hcy. Conclusion: Hcy induces osteoblast differentiation of fibroblasts in vitro. Its mechanism and relationship to calcified lesions in vivo should be investigated further.
2P-0380
Serum homocysteine and MCP-1 are associated with abdominal aortic aneurysm size and interluminal thrombus dimension
B. Millo, I. Wiernicki, H. Bukowska, B. Gorecka-Szyld, K. Chelstowski, M. Naruszewicz. Pomeranian Medical University, Szczecin, Poland Objective: The risk of nonaneurysm cardiovascular mortality before and after surgery increased with abdominal aortic aneurysms (AAA) diameter. Surgery of aneurysm with diameter smaller than 5 cm is not advised. Chronic inflammation is observed in patients with AAA but both clinical and biochemical assessment remains insufficient. The purpose of this study was to examine if elevated biochemical markers: homocysteine (HCY), lipoprotein a (Lp(a)), monocyte chemotactic protein 1 (MCP-1), C-reactive protein (CRP), total cholesterol (Ch), HDL-Ch, oxidized LDL (Oxy-LDL), triglyceride (Tg) can distinguish patients with higher risk of cardiovascular diseases. Methods: We examined 31 patients divided according aneurysm size: I gr. >5 cm (n=15) and II gr. <5 cm (n=16). Mean age was: 70.8±6.8 in gr I; 67.7±8.6 in gr II (NS). AAA diameter as well as interluminal thrombus dimension (ITD) were assessed by an abdominal USG and MRI or CT. HCY levels were determined by HPLC; MCP-1, Oxy-LDL by ELISA and CRP by high sensitivity ELISA technique. Results: Mean AAA size was 6.08±1.01 cm in gr. I, 4.00±0.54 cm in gr. II (p<0.01). Mean ITD was 1.16±0.60 cm in gr. I, 0.59±0.32 cm in gr. II (p<0.01). Mean HCY was 18.42±4.21 µmol/L in gr. I, 14.78±3.36 µmol/L in gr. II (p<0.01). HCY levels correlated significantly with the ITD (r=0.42); MCP-1 with the ITD and with AAA diameter (r=0.57 and r=0.45, respectively). Conclusion: HCY and MCP-1 levels may be useful in management of patients with AAA which are at high risk of cardiovascular mortality. 2P-0381
The assessment of the influence of natural coffee and its modified form on the level of homocysteine, vitamin B6 and folic acid in healthy volunteers
H. Bukowska, I. Goracy, K. Chelstowski, M. Naruszewicz. Department of Clinical Biochemistry and Laboratory Diagnostics, Pomeranian Medical University, Szczecin, Poland Objective: An elevated level of plasma homocysteine is a know risk factor for atherosclerotic vascular diseases. The aim of our investigation was the assessment of the influence of natural coffee and that modified by water and pressure extraction (60% less of 2-methyl isoborneol) on the level of homocysteine, folic acid and vitamin B6 in healthy volunteers. Methods: The study was conducted on 36 healthy volunteers: 20 women and 16 men; smokers constituted half of the group. The study was conducted as a double blind trial (coffee without labels) after randomization into two groups. Initially one group drank natural coffee and the other a modified one. After four weeks there was a 28-day break in drinking coffee, after which the groups swapped roles and another trial lasted for the subsequent 4 weeks. All people examined drank three servings of coffee a day brewed from 13g of material in 180 ml of boiling water. Throughout the entire experiment the examined subjects did not change their diets and did not take any vitamin supplements. Blood for analysis was drawn four times and the following analyses were carried out: homocysteine, folic acid, vitamin B6, total, LDL and HDL-cholesterol, triglicerides, Lp(a) and fibrinogen. Results: After 4 weeks of drinking of both kinds of coffee we did not observe any significant changes in the body weight, concentration of analyzed lipid parameters, fibrinogen, folic acid and vitamin B6. We found a significant increase level of plasma homocysteine from 9.6 to 11.4 mikromol (p<0.001) in persons drinking natural, unfiltered coffee. However drinking modified coffee free from irritants resulted in a tendency towards lowering the level of homocysteine (from 9.1 to 8.7 mikromol). Conclusion: From the above study it may be concluded that lowering the content of isoborneol in coffee results in reducing its undesired influence on the homocysteine level. 2P-0382
Homocysteine and premature coronary heart disease
M. Ezhov, V. Naumov, M. Afanasieva, S. Pokrovsky. Cardiology Research Center, Russia The aim of our study was to assess homocysteine (Hcy) level in patients with premature coronary heart disease (CHD). Material and Methods: We examined 32 CHD patients (30 men, 2 women, XIIIth International Symposium on Atherosclerosis, September 28–October 2, 2003, Kyoto, Japan
Tuesday September 30, 2003: Poster Session Homocysteines and other risk factors
HDL-Cholesterol (HDL-C), LDL-Cholesterol (LDL-C) and folic acid before and after administration folic acid (Filicine) 5 mg/d. The patients had well nephritic and hepatic function and had not neoplasia or megaloblastic anemia and diabetes mellitus. There were no complications from folic acid administration. Compared the effects before (b) versus after (a) treatment. All results were analyzed in the same laboratory. Results: Hcy 15.9±6.1 (b) -11.0±2.8 (a) µmol/l (p=0.01), Folic 7.9±5.8 (b) -19.3±3.0 (a) ng/ml (p=0.095), TC 225±39 (b) -229±27 (a) mg/dl (p=0.30), TG 153±82 (b) -127±42 (a) mg/dl (p=0.002), HDL 57.3±12.8 (b) -60.1±15.3 (b) mg/dl (p=,000), LDL 137±31 (b) -141±25 (a) mg/dl (p=0.53). Statistical analysis was performed using a SPSS 11.0. Conclusion: The study shows that in patients with hyperhomocysteinemia the administration 5 mg/d folic acid for 4 weeks a) decreased the homocysteine levels statistically significant, b) the TG levels decreased statistically significant, and c) the HDL cholesterol levels increased statistically significant. 2P-0378
Plasma homocysteine in an Australian population sample
P. Roach 1 , N. Naumovski 1 , B. Blades 1 , P. Lewis 2 . 1 University of Newcastle; 2 Central Coast Health Public Health Unit, Australia Objectives: To determine the plasma homocysteine concentrations in a sample of the New South Wales Central Coast population and relate them to their red cell folate and plasma vitamin B12 levels. Methods: Plasma samples were collected from 387 subjects (213 females; 174 males), with a mean age of 54.6 ± 15.5 years (mean ± sd) (females 54.2 ± 15.3; males 55.2 ± 15.8). Homocysteine was measured by HPLC. Red cell folate and vitamin B12 were measured by immunoassay. Results: The mean homocysteine concentrations were 10.3 ± 5.2 µM for the whole population sample, 10.0 ± 5.7 µM for the females and 10.6 ± 4.5 µM for the males. Notably, 14% of subjects had plasma homocysteine concentrations higher than 14 µM and 40% had concentrations higher than 10 µM. There was a positive correlation between the homocysteine concentration and age (r = 0.160; p = 0.002), a negative correlation between the homocysteine concentration and red cell folate (r = -0.247; p<0.001) and a negative correlation between the homocysteine concentration and plasma vitamin B12 levels (r = -0.230; p<0.001). Conclusions: This sample of 387 subjects had plasma homocysteine concentrations similar to other population samples with 40% having levels above the recommended 10 µM threshold. Increasing age, decreased red cell folate and decreased plasma vitamin B12 appeared to be factors contributing to increased plasma homocysteine concentrations in this population sample. 2P-0379
Homocysteine induces osteoblast differentiation of fibroblasts in vitro
Y. Kusumi, S. Sueyoshi, M. Niihashi, M. Mitsumata. Department of Pathology, Nihon University School of Medicine, Tokyo, Japan Objective: Recent evidence demonstrates that hyperhomocysteinaemia is an independent risk factor for cardiovascular diseases. Because atherogenic disorders such as diabetes and uremia, frequently showing calcified arterial lesions, also tend to have increased plasma homocysteine (Hcy) levels, we hypotheized that Hcy induces osteoblast differentiation of some vascular cells including fibroblasts. Methods: Human fibroblasts were cultured, and after subconfluent they were exposed to various concetrations of Hcy (2 mM to 8 mM) in medium. Their morphological and biochemical changes were followed for 28 days. Results: By 14 days after homocysteine at 4 mM or 6 mM, cells formed typical nodules that have similar morphology as mineralized nodules of smooth muscle cells reported by others. By histochemical staining and chemical measurement it was shown that homocysteine induces increased alkaline phosphatase activity compared with control. von Kossa staining showed calcium depositions predominantly in these nodules of cultured cells under Hcy. Conclusion: Hcy induces osteoblast differentiation of fibroblasts in vitro. Its mechanism and relationship to calcified lesions in vivo should be investigated further.
2P-0380
Serum homocysteine and MCP-1 are associated with abdominal aortic aneurysm size and interluminal thrombus dimension
B. Millo, I. Wiernicki, H. Bukowska, B. Gorecka-Szyld, K. Chelstowski, M. Naruszewicz. Pomeranian Medical University, Szczecin, Poland Objective: The risk of nonaneurysm cardiovascular mortality before and after surgery increased with abdominal aortic aneurysms (AAA) diameter. Surgery of aneurysm with diameter smaller than 5 cm is not advised. Chronic inflammation is observed in patients with AAA but both clinical and biochemical assessment remains insufficient. The purpose of this study was to examine if elevated biochemical markers: homocysteine (HCY), lipoprotein a (Lp(a)), monocyte chemotactic protein 1 (MCP-1), C-reactive protein (CRP), total cholesterol (Ch), HDL-Ch, oxidized LDL (Oxy-LDL), triglyceride (Tg) can distinguish patients with higher risk of cardiovascular diseases. Methods: We examined 31 patients divided according aneurysm size: I gr. >5 cm (n=15) and II gr. <5 cm (n=16). Mean age was: 70.8±6.8 in gr I; 67.7±8.6 in gr II (NS). AAA diameter as well as interluminal thrombus dimension (ITD) were assessed by an abdominal USG and MRI or CT. HCY levels were determined by HPLC; MCP-1, Oxy-LDL by ELISA and CRP by high sensitivity ELISA technique. Results: Mean AAA size was 6.08±1.01 cm in gr. I, 4.00±0.54 cm in gr. II (p<0.01). Mean ITD was 1.16±0.60 cm in gr. I, 0.59±0.32 cm in gr. II (p<0.01). Mean HCY was 18.42±4.21 µmol/L in gr. I, 14.78±3.36 µmol/L in gr. II (p<0.01). HCY levels correlated significantly with the ITD (r=0.42); MCP-1 with the ITD and with AAA diameter (r=0.57 and r=0.45, respectively). Conclusion: HCY and MCP-1 levels may be useful in management of patients with AAA which are at high risk of cardiovascular mortality. 2P-0381
The assessment of the influence of natural coffee and its modified form on the level of homocysteine, vitamin B6 and folic acid in healthy volunteers
H. Bukowska, I. Goracy, K. Chelstowski, M. Naruszewicz. Department of Clinical Biochemistry and Laboratory Diagnostics, Pomeranian Medical University, Szczecin, Poland Objective: An elevated level of plasma homocysteine is a know risk factor for atherosclerotic vascular diseases. The aim of our investigation was the assessment of the influence of natural coffee and that modified by water and pressure extraction (60% less of 2-methyl isoborneol) on the level of homocysteine, folic acid and vitamin B6 in healthy volunteers. Methods: The study was conducted on 36 healthy volunteers: 20 women and 16 men; smokers constituted half of the group. The study was conducted as a double blind trial (coffee without labels) after randomization into two groups. Initially one group drank natural coffee and the other a modified one. After four weeks there was a 28-day break in drinking coffee, after which the groups swapped roles and another trial lasted for the subsequent 4 weeks. All people examined drank three servings of coffee a day brewed from 13g of material in 180 ml of boiling water. Throughout the entire experiment the examined subjects did not change their diets and did not take any vitamin supplements. Blood for analysis was drawn four times and the following analyses were carried out: homocysteine, folic acid, vitamin B6, total, LDL and HDL-cholesterol, triglicerides, Lp(a) and fibrinogen. Results: After 4 weeks of drinking of both kinds of coffee we did not observe any significant changes in the body weight, concentration of analyzed lipid parameters, fibrinogen, folic acid and vitamin B6. We found a significant increase level of plasma homocysteine from 9.6 to 11.4 mikromol (p<0.001) in persons drinking natural, unfiltered coffee. However drinking modified coffee free from irritants resulted in a tendency towards lowering the level of homocysteine (from 9.1 to 8.7 mikromol). Conclusion: From the above study it may be concluded that lowering the content of isoborneol in coffee results in reducing its undesired influence on the homocysteine level. 2P-0382
Homocysteine and premature coronary heart disease
M. Ezhov, V. Naumov, M. Afanasieva, S. Pokrovsky. Cardiology Research Center, Russia The aim of our study was to assess homocysteine (Hcy) level in patients with premature coronary heart disease (CHD). Material and Methods: We examined 32 CHD patients (30 men, 2 women, XIIIth International Symposium on Atherosclerosis, September 28–October 2, 2003, Kyoto, Japan