3p21 and 3q25 breakpoints successively involved in a philadelphia-positive chronic myelocytic leukemia with thrombocythemia

LETTERS TO THE EDITOR 3p21 and 3q25 Breakpoints Successively Involved in a Philadelphia-Positive Chronic Myelocytic Leukemia With Thrombocythemia We have previously reported a case of chronic myelocytic leukemia (CML) with the translocation t(3;20)(p21;p13), associated with t h r o m b o c y t h e m i a [1]. After our report appeared, the patient d e v e l o p e d a blastic crisis with persistence of the hyperthrombocytosis. The karyotype n o w presented a n e w and c o m p l e x translocation involving the der(3) and c h r o m o s o m e s #15 and # 1 7 (Figs. 1 and 2). Thus, the karyotype was shown to be 46,XY,t(9;22)(q34;q11)t(3;20) (p21;13) t[der(3);15;17] (q25;q22;q21). C h e m o t h e r a p y was a d m i n i s t e r e d (azacytidine and VP16); however, the patient's c o n d i t i o n deteriorated r a p i d l y and he d i e d of infection. The new breakpoint of c h r o m o s o m e #3, (3q25), is to be considered in relation to reports in the literature [2-4], relating it to t h r o m b o c y t h e m i c aspects of hematologic disorders. The i n v o l v e m e n t of chromosomes #15 and #17 also are of interest. M.F. TURCHINI P. TRAVADE A. GENEIX A. DE LAROQUE B. PERISSEL P. MALET

Laboratoire de Cytog6n6tique Facult~ de Medecine C.H.R. de Clermont Ferrand France Laboratoire d'H~matologie

The authors thank Dr. A. Hagemeijer (Erasmus University, Rotterdam) for confirming our results with her technique. Received June 16, 1986; accepted July 21, 1986.

371 Science Publishing Co., Inc. 52 Vanderbilt Ave., New York, NY 10017

© 1987 Elsevier

Cancer Genet Cytogenet 27:371-374 (1987) 0165-4608/87/$03.50

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REFERENCES 1. Turchini MF, Travade P, De Larocque A, Geneix A, Perissel B, Malet P (1986): Translocation (3;20) associated with thrombocythemia in Ph-positive CML. Cancer Genet Cytogenet 20:1-4. 2. Shimazaki C, Fujita N, Nakanishi S, Nishio A, Haruyama H, Nakagawa M, Ijichi H, Nishida K, Misawa S (1986): Inversion of chromosome 3 in a case of chronic myelogenous leukemia with abnormal thrombopoiesis. Cancer Genet Cytogenet 20:121-127. 3. Mecucci C, Vermaelen K, Tricot G, Louwagie A, Michaux J, Boslv A, Thomas J, Barbieri D, Van den Berghe H (1982): 3 q - 3q + anomaly in malignant proliferations in humans. Cancer Genet Cytogenet 8:367-381. 4. Pintado T, Ferro MT, San Roman C, Mayayo M, Larafia JG (1985): Clinical correlations of the 3q21:q26 cytogenetic anomaly. Cancer 55:535-541.