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4859465 Vaccine capable of eliciting multivalent antibodies
PATENT ABSTRACTS 4859465 VACCINE CAPABLE OF ELICITING MULTIVALENT ANTIBODIES William J Rutter assigned to The Regents of the University of California The present invention discloses a DNA transfer vector comprising two or more deoxynucleotide sequences coding for different antigenic materials linked together in phase with one another. The present invention further discloses the expression of said deoxynucleotide sequences either directly or as a fusion protein with the product of a procaryotic gene. The resulting expression product is then either a fusion protein comprising two or more antigenic materials or a fusion protein comprising a part of a procaryotic protein and two or more antigenic materials. These fusion products are capable of elicitingthe formation ofmultivalentantibodies which are cross-reactive with any and all of the native antigenic material. A vaccine is also described utilizing these fusion products.
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4859596 CLONING SYSTEM FOR KLUYVEROMYCES SPECIES Cornelis HoUenberg, Suni Das, Albert De Leeuw, Berg Johannes van den, Dusseldorf, Federal Republic Of Germany assigned to GistBrocades N V A new cloning system is described capable of expressing genetic material derived from recombinant DNA material, which comprises a yeast of the genus Kluyveromyces as a host. Suitable vectors are e.g. vectors containing autonomously replicating sequences (ARS) and vectors containing homologous Khiyveromyces DNA acting as a site t~or recombination with the host chromosome. New and preferred vectors are those containing ARS sequences originating from Kluyveromyces (KARS vectors). The genetically engineered new strains of Kluyveromyees produce, inter alia, lactase and chymosin. 4859601 STREPTOMYCES BGL PROTEIN GENE PROMOTER William Burnett, Thomas G Eckhardt, Louis R Fare assigned to SmithKline Beckman Corporation
4859587 RECOMBINANT HERPES SIMPLEX VIRUSES, VACCINES AND METHODS Bernard Ruizman assigned to Institut Merieux Recombinant herpes viruses useful as effective ingredients in vaccines against both virulent HSV- i and HSV-2 are disclosed. Methods of preparing the recombinant viruses, vaccines incorporating the viruses, and methods of immunizing a human host by inoculation v,,ith the vaccines are also disclosed. The genomes of the recombinant viruses each comprise a mutant of the HSV-I genome from which a portion thereof responsible for neurovirulence yet nonessential for growth is deleted. A gene from the HSV-2 genome responsible for coding an immunityinducing giycoprotein is inserted in the mutant genome between the end points of deletion therein,
A DNA fragment from Streptomyces sp. which contains the Bgl protein (P49) promoter, the P1 promoter, for expressing heterologous genes. 4861448 ELECTROPHORETIC METHODS. EMPLOYING GEL INSERTS Charles R Cantor, David C Schwartz assigned to The Trustees of Columbia University in the City of New York Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as D N A or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separat.ions. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.
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4859596 CLONING SYSTEM FOR KLUYVEROMYCES SPECIES Cornelis HoUenberg, Suni Das, Albert De Leeuw, Berg Johannes van den, Dusseldorf, Federal Republic Of Germany assigned to GistBrocades N V A new cloning system is described capable of expressing genetic material derived from recombinant DNA material, which comprises a yeast of the genus Kluyveromyces as a host. Suitable vectors are e.g. vectors containing autonomously replicating sequences (ARS) and vectors containing homologous Khiyveromyces DNA acting as a site t~or recombination with the host chromosome. New and preferred vectors are those containing ARS sequences originating from Kluyveromyces (KARS vectors). The genetically engineered new strains of Kluyveromyees produce, inter alia, lactase and chymosin. 4859601 STREPTOMYCES BGL PROTEIN GENE PROMOTER William Burnett, Thomas G Eckhardt, Louis R Fare assigned to SmithKline Beckman Corporation
4859587 RECOMBINANT HERPES SIMPLEX VIRUSES, VACCINES AND METHODS Bernard Ruizman assigned to Institut Merieux Recombinant herpes viruses useful as effective ingredients in vaccines against both virulent HSV- i and HSV-2 are disclosed. Methods of preparing the recombinant viruses, vaccines incorporating the viruses, and methods of immunizing a human host by inoculation v,,ith the vaccines are also disclosed. The genomes of the recombinant viruses each comprise a mutant of the HSV-I genome from which a portion thereof responsible for neurovirulence yet nonessential for growth is deleted. A gene from the HSV-2 genome responsible for coding an immunityinducing giycoprotein is inserted in the mutant genome between the end points of deletion therein,
A DNA fragment from Streptomyces sp. which contains the Bgl protein (P49) promoter, the P1 promoter, for expressing heterologous genes. 4861448 ELECTROPHORETIC METHODS. EMPLOYING GEL INSERTS Charles R Cantor, David C Schwartz assigned to The Trustees of Columbia University in the City of New York Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as D N A or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separat.ions. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.