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4873187 Bifunctional DNA-protein conjugating agent
PATENT ABSTRACTS
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other to form resolved rows on a support medium, which comprises a process including: (I) determining on each of the resolved rows a scanning line for signal processing; (2) detecting on each of the resolved rows sampling points on said scanning line; and (3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, ademine, thymine and cytosine; said process being applied to digital signals corresponding to an autoradiograph having the locational information on the radioactively labeled synthetic products, said digital signals being obtained by the use of a stimulable phosphor sheet or by the use of a radiosensitive material.
sequence homologies to immunoglobulin V and C genes. Both the T cell receptor protein and its subunits may be produced from the cDNA clones. The protein molecules may be further used for the production of T-cell clone specific antibodies. 4873191 GENETIC TRANSFORMATION ZYGOTES
OF
Thomas E Wagner, Peter Hoppe assigned to Ohio University
4873190
Genetic transformation of a zygote and the embryo and mature organism which result therefrom is obtained by placing or inserting exogenous genetic material into the nucleus of the zygote or into any genetic material which ultimately forms at least a part of the nucleus of the zygote. It is preferred that the exogenous genetic material be added to a pronuclei of the zygote and is particularly preferred that it be added to the male pronucleus of the zygote. Thereafter, the zygote is allowed to undergo differentiation and development into the organism. The genotype of the zygote and the organism which results therefrom will include the genotype of the exogenous genetic material and the exogenous genetic material will be phenotypically expressed. The invention can be utilized in a variety of ways including, for example, animal and plant breeding to modify or create new species, it can be used in ¢pigenetics and in the understanding and treatment of genetic diseases.
HETERODIMERIC T LYMPHOCYTE RECEPTOR
4873192
4873187 BIFUNCTIONAL DNA-PROTEIN CONJUGATING AGENT Floy Taub assigned to Digene Diagnostics Incorporated A cationic detergent having a positively charged group and a hydrophobic group is used to conjugate an enzymatically active enzyme molecule with a single-stranded nucleic acid molecule. The conjugate may be used to detect the presence of nucleic acid molecules having a nucleotide sequence which is complementary to that of the single-stranded molecule of the conjugate,
Haruo Saito, David Kranz, Herman N Eisen, Susumu Tonegawa assigned to Massachusetts Institute of Technology
PROCESS FOR SITE SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION
Disclosed is a beterodimeric T lymphocyte receptor comprising an alpha and a beta subunit, Each subunit consists of a signal peptide, variable, joining, constant, transmembrane, and cytoplasmic regions. The two subunits are connected by a disulfide bond between cysteine residues located between the constant and transmembrane region. The structure, amino acid, and nucleotide sequence of the lymphocyte receptor were determined using cDNA clones derived from a functional murine cytotoxic T lymphocyte clone. The genes corresponding to these cDNA are expressed and rearranged specifically in T cells and have significant
Thomas A Kunkelassigned to The United States of America as represented by the Department of Health and Human Services The present invention discloses several DNA mutagenesis processes using a DNA template containing several uracil residues in place of thymine, which can be applied without selection techniques to produce altered DNA sequences with approximately 10-fold greater efficiency than current methods of site-specific mutagenesis. This template has relatively normal coding potential in the in vitro reactions typical of standard site-directed mutagenesis protocols
37~
other to form resolved rows on a support medium, which comprises a process including: (I) determining on each of the resolved rows a scanning line for signal processing; (2) detecting on each of the resolved rows sampling points on said scanning line; and (3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, ademine, thymine and cytosine; said process being applied to digital signals corresponding to an autoradiograph having the locational information on the radioactively labeled synthetic products, said digital signals being obtained by the use of a stimulable phosphor sheet or by the use of a radiosensitive material.
sequence homologies to immunoglobulin V and C genes. Both the T cell receptor protein and its subunits may be produced from the cDNA clones. The protein molecules may be further used for the production of T-cell clone specific antibodies. 4873191 GENETIC TRANSFORMATION ZYGOTES
OF
Thomas E Wagner, Peter Hoppe assigned to Ohio University
4873190
Genetic transformation of a zygote and the embryo and mature organism which result therefrom is obtained by placing or inserting exogenous genetic material into the nucleus of the zygote or into any genetic material which ultimately forms at least a part of the nucleus of the zygote. It is preferred that the exogenous genetic material be added to a pronuclei of the zygote and is particularly preferred that it be added to the male pronucleus of the zygote. Thereafter, the zygote is allowed to undergo differentiation and development into the organism. The genotype of the zygote and the organism which results therefrom will include the genotype of the exogenous genetic material and the exogenous genetic material will be phenotypically expressed. The invention can be utilized in a variety of ways including, for example, animal and plant breeding to modify or create new species, it can be used in ¢pigenetics and in the understanding and treatment of genetic diseases.
HETERODIMERIC T LYMPHOCYTE RECEPTOR
4873192
4873187 BIFUNCTIONAL DNA-PROTEIN CONJUGATING AGENT Floy Taub assigned to Digene Diagnostics Incorporated A cationic detergent having a positively charged group and a hydrophobic group is used to conjugate an enzymatically active enzyme molecule with a single-stranded nucleic acid molecule. The conjugate may be used to detect the presence of nucleic acid molecules having a nucleotide sequence which is complementary to that of the single-stranded molecule of the conjugate,
Haruo Saito, David Kranz, Herman N Eisen, Susumu Tonegawa assigned to Massachusetts Institute of Technology
PROCESS FOR SITE SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION
Disclosed is a beterodimeric T lymphocyte receptor comprising an alpha and a beta subunit, Each subunit consists of a signal peptide, variable, joining, constant, transmembrane, and cytoplasmic regions. The two subunits are connected by a disulfide bond between cysteine residues located between the constant and transmembrane region. The structure, amino acid, and nucleotide sequence of the lymphocyte receptor were determined using cDNA clones derived from a functional murine cytotoxic T lymphocyte clone. The genes corresponding to these cDNA are expressed and rearranged specifically in T cells and have significant
Thomas A Kunkelassigned to The United States of America as represented by the Department of Health and Human Services The present invention discloses several DNA mutagenesis processes using a DNA template containing several uracil residues in place of thymine, which can be applied without selection techniques to produce altered DNA sequences with approximately 10-fold greater efficiency than current methods of site-specific mutagenesis. This template has relatively normal coding potential in the in vitro reactions typical of standard site-directed mutagenesis protocols