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5008192 Enzymatic process for the preparation of optically active cyanohydrins
499
PATENT ABSTRACTS and amphoteric surfactants, and a cationically modified hydrolyzed collagen stabilizer, which stabilizer the dextranase in the presence of the anionic surfactant without substantially reducing its foaming and cleansing properties.
5002766 USE OF CATABOLIC ENZYMES FOR CONTROLLING THE ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS) AND ITS PRECURSORS (LAS, ARC) Karl Ransberger, Gerhard Stauder, Gr Federal Republic Of Germana assigned to Mucos Pharma GmbH & Co The therapy approaches known so far for the treatment of the acquired immune deficiency syndrome (AIDS) and its precursors (LAS and ARC) and the secondary diseases related thereto such as opportunistic infections and malignant tumours are either directed against the proliferation of the virus in the body or against the pathogenic organisms of the opportunistic infections or they are selected from the known agents against malignant tumours. So far all therapies did not have any significant success. The new use of catabolic enzymes for the therapy of AIDS and its precursors (LAS and ARC) is made available, with which completely unexpected successes can be achieved in the improvement of the condition of the patient.
5002871 ENZYMATIC MEMBRANE METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES Guillermo A lacobucci assigned to The CocaCola Company The present invention provides a membrane method for the enzymatic synthesis of peptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized reacting amino acids and uncharged or non-ionized peptide product in the presence ofa proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the unchanged peptide product across an ionrejection membrane which removes the uncharged peptide from the reaction mixture
and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively pulled across the membrane. An enzymatic conversion of the uncharged species utilizing an esterase having proteolytic activity such as aminoacylase I is disclosed. Copermeating reactants can be separated from the product mixture and returned to the reaction mixture. Also, the ion-rejection membrane can be utilized to resolve enantiomers of racemic carboxylic acids including D, L-amino acids.
5002872 ENZYME
MEDIATED COUPLING REACTIONS
Akiva T Gross assigned to W R Grace & Co Corm Disclosed herein is an enzymatic process to bind two amino acids in the presence of a watermi~ible solvent. Preferably the amino acids are precursors of aspartame and a preferred solvent is acetonitrile.
5002880 ENZYME CATALYZED SYNTHESIS OF METHYL URETHANES Robert W Mason assigned to Olin Corporation The present invention relates to a process for making an isocyanate which comprises the steps of: (a) reacting an amine with dimethyl carbonate in the presence of a hydrolase enzyme catalyst, preferably at a temperature not exceeding 50 degrees C. to form a methyl urethane, and (b) subjecting said methyl urethane to an elevated temperature in order to thermally cleave the urethane to produce an isocyanate.
5008192 ENZYMATIC PROCESS FOR THE PREPARATION OF OPTICALLY ACTIVE CYANOHYDRINS Uw Neidermeyer, Udo Kragl, Maria R Kula, Christia Wandrey, Kyriakos Makrylaleas, Karlheinz Drauz, Walberberg, Federal Republic Of Germany assigned to Kernforschungsanlage Juelich GmbH; Degussa Aktiengesellscha
PATENT ABSTRACTS
500
An enzymatic reaction of aliphatic, aromatic or heteroaromatic aldehydes or ketones with hydrocyanic acid to form the corresponding (R)cyanohydrins or (S)-cyanohydrins is carried out in an aqueous medium in the presence of (R)oxynitrilase (4.1.2.10) or oxynitrilase (4.1.2.1 I) under acid conditions and at temperatures such that the competing chemical reaction and racemization are negligible compared with the enzymatic synthesis. The process is preferably carried out at a pH of< or =4.5 and at reaction temperatures between -5 degrees C. and +50 degrees C. The reaction is preferably carried out continuously, advantageously in an enzyme membrane reactor. Of particular interest is the conversion of aromatic aldehydes into the corresponding optically active cyanohydrins.
5008193 ASCORBIC ACID INTERMEDIATES AND PROCESS ENZYMES Stephen Anderson, David R Light, Car Marks, William H Rastetter assigned to Genentech Inc Methods for producing ascorbic acid using recombinant means comprising the transfer of genetic material by conjugation, a host cell lacking, entirely or to such an extent as not to be commercially useful, one or more enzymes in the metabolic path converting glucose to 2 keto-Lgulonic acid.
5008245 NOVEL PEPTIDYL CARBAMATE INHIBITORS OF THE ENZYME ELASTASE
treatment comprises administering to the animal or human an enzyme elastase inhibiting amount of one of the compounds of the invention or a composition thereof.
5013648 ENZYMATIC DETECTION OF MONOVALENT ANIONS John Pasqua, Wai T Law assigned to EM Diagnostic Systems lnc An enzymatic reagent, kit and method for determining the concentration of monovalent anions, especially chloride, particularly in clinical samples, and especially in a single-reagent format suitable for automated clinical chemistry analyzers. 5017475 FLUORESCENT DETECTION METHOD BASED ON ENZYME ACTIVATED CONVERSION OF A FLUOROPHORE PRECURSOR SUBSTRATE Richard A Harte, Stephen H Mastin assigned to Microbiological Associates Inc Methods are provided for the fluorescent detection of an analyte of interest wherein an aminesubstituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.
George A Digenis, Bushra J Agha assigned to University of Kentucky Research Foundation
50~9~
Compounds selected from the group consisting of a compound of the formula See Patent for Chemical Structure (I) and compound of the formula See Patent for Chemical Structure (ii) wherein x is 1 or 2, Y is carbobenzoxy or benzoyl, and XR is See Patent for Chemical Structure have use as elastase enzyme inhibitors. Particularly potent are the L-proline diastereomers. Elastase Enzyme inhibitory compositions comprise a carrier and an elastase enzyme inhibiting amount of the compounds of the invention. A method of selectively inhibiting the enzyme elastase in an animal or a human in need of such
PROCESS FOR OBTAINING SARCOSINE OXIDASE FROM MICROORGANISMS Ulrich Mayr, Helmgar Gauhl, Hans Seidel, Rosenheim, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH The present invention provides a process for obtaining sarcosine oxidase from micro-organisms by culturing thereof and obtaining the enzyme from the biomass or from the culture broth, wherein a Pseudomonas strain is cultured.
PATENT ABSTRACTS and amphoteric surfactants, and a cationically modified hydrolyzed collagen stabilizer, which stabilizer the dextranase in the presence of the anionic surfactant without substantially reducing its foaming and cleansing properties.
5002766 USE OF CATABOLIC ENZYMES FOR CONTROLLING THE ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS) AND ITS PRECURSORS (LAS, ARC) Karl Ransberger, Gerhard Stauder, Gr Federal Republic Of Germana assigned to Mucos Pharma GmbH & Co The therapy approaches known so far for the treatment of the acquired immune deficiency syndrome (AIDS) and its precursors (LAS and ARC) and the secondary diseases related thereto such as opportunistic infections and malignant tumours are either directed against the proliferation of the virus in the body or against the pathogenic organisms of the opportunistic infections or they are selected from the known agents against malignant tumours. So far all therapies did not have any significant success. The new use of catabolic enzymes for the therapy of AIDS and its precursors (LAS and ARC) is made available, with which completely unexpected successes can be achieved in the improvement of the condition of the patient.
5002871 ENZYMATIC MEMBRANE METHOD FOR THE SYNTHESIS AND SEPARATION OF PEPTIDES Guillermo A lacobucci assigned to The CocaCola Company The present invention provides a membrane method for the enzymatic synthesis of peptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized reacting amino acids and uncharged or non-ionized peptide product in the presence ofa proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the unchanged peptide product across an ionrejection membrane which removes the uncharged peptide from the reaction mixture
and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively pulled across the membrane. An enzymatic conversion of the uncharged species utilizing an esterase having proteolytic activity such as aminoacylase I is disclosed. Copermeating reactants can be separated from the product mixture and returned to the reaction mixture. Also, the ion-rejection membrane can be utilized to resolve enantiomers of racemic carboxylic acids including D, L-amino acids.
5002872 ENZYME
MEDIATED COUPLING REACTIONS
Akiva T Gross assigned to W R Grace & Co Corm Disclosed herein is an enzymatic process to bind two amino acids in the presence of a watermi~ible solvent. Preferably the amino acids are precursors of aspartame and a preferred solvent is acetonitrile.
5002880 ENZYME CATALYZED SYNTHESIS OF METHYL URETHANES Robert W Mason assigned to Olin Corporation The present invention relates to a process for making an isocyanate which comprises the steps of: (a) reacting an amine with dimethyl carbonate in the presence of a hydrolase enzyme catalyst, preferably at a temperature not exceeding 50 degrees C. to form a methyl urethane, and (b) subjecting said methyl urethane to an elevated temperature in order to thermally cleave the urethane to produce an isocyanate.
5008192 ENZYMATIC PROCESS FOR THE PREPARATION OF OPTICALLY ACTIVE CYANOHYDRINS Uw Neidermeyer, Udo Kragl, Maria R Kula, Christia Wandrey, Kyriakos Makrylaleas, Karlheinz Drauz, Walberberg, Federal Republic Of Germany assigned to Kernforschungsanlage Juelich GmbH; Degussa Aktiengesellscha
PATENT ABSTRACTS
500
An enzymatic reaction of aliphatic, aromatic or heteroaromatic aldehydes or ketones with hydrocyanic acid to form the corresponding (R)cyanohydrins or (S)-cyanohydrins is carried out in an aqueous medium in the presence of (R)oxynitrilase (4.1.2.10) or oxynitrilase (4.1.2.1 I) under acid conditions and at temperatures such that the competing chemical reaction and racemization are negligible compared with the enzymatic synthesis. The process is preferably carried out at a pH of< or =4.5 and at reaction temperatures between -5 degrees C. and +50 degrees C. The reaction is preferably carried out continuously, advantageously in an enzyme membrane reactor. Of particular interest is the conversion of aromatic aldehydes into the corresponding optically active cyanohydrins.
5008193 ASCORBIC ACID INTERMEDIATES AND PROCESS ENZYMES Stephen Anderson, David R Light, Car Marks, William H Rastetter assigned to Genentech Inc Methods for producing ascorbic acid using recombinant means comprising the transfer of genetic material by conjugation, a host cell lacking, entirely or to such an extent as not to be commercially useful, one or more enzymes in the metabolic path converting glucose to 2 keto-Lgulonic acid.
5008245 NOVEL PEPTIDYL CARBAMATE INHIBITORS OF THE ENZYME ELASTASE
treatment comprises administering to the animal or human an enzyme elastase inhibiting amount of one of the compounds of the invention or a composition thereof.
5013648 ENZYMATIC DETECTION OF MONOVALENT ANIONS John Pasqua, Wai T Law assigned to EM Diagnostic Systems lnc An enzymatic reagent, kit and method for determining the concentration of monovalent anions, especially chloride, particularly in clinical samples, and especially in a single-reagent format suitable for automated clinical chemistry analyzers. 5017475 FLUORESCENT DETECTION METHOD BASED ON ENZYME ACTIVATED CONVERSION OF A FLUOROPHORE PRECURSOR SUBSTRATE Richard A Harte, Stephen H Mastin assigned to Microbiological Associates Inc Methods are provided for the fluorescent detection of an analyte of interest wherein an aminesubstituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.
George A Digenis, Bushra J Agha assigned to University of Kentucky Research Foundation
50~9~
Compounds selected from the group consisting of a compound of the formula See Patent for Chemical Structure (I) and compound of the formula See Patent for Chemical Structure (ii) wherein x is 1 or 2, Y is carbobenzoxy or benzoyl, and XR is See Patent for Chemical Structure have use as elastase enzyme inhibitors. Particularly potent are the L-proline diastereomers. Elastase Enzyme inhibitory compositions comprise a carrier and an elastase enzyme inhibiting amount of the compounds of the invention. A method of selectively inhibiting the enzyme elastase in an animal or a human in need of such
PROCESS FOR OBTAINING SARCOSINE OXIDASE FROM MICROORGANISMS Ulrich Mayr, Helmgar Gauhl, Hans Seidel, Rosenheim, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH The present invention provides a process for obtaining sarcosine oxidase from micro-organisms by culturing thereof and obtaining the enzyme from the biomass or from the culture broth, wherein a Pseudomonas strain is cultured.