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5100794 Amylolytic enzymes producing microorganisms, constructed by recombinant DNA technology and their use for permentation processes
PATENTABSTRACTS
5100794 AMYLOLYTIC ENZYMES PRODUCING MICROORGANISMS, CONSTRUCTED BY RECOMBINANT DNA TECHNOLOGY AND THEIR USE FOR PERMENTATION PROCESSES Alexander Strasser, Feodor B Martens, Jurge Dohmen, Cornelius P Hollenberg, Dusseldorf, Federal Republic Of Germany The cloning of Schwanniomyces glucoamytase and alpha-amylase genes is taught. The genes are expressed in recombinant host cells.
5100798 METHOD FOR THE EXTRACTION OF A VITAMIN-K-DEPENDENT CARBOXYLASE COMPLEX AND THE USE THEREOF Cornelis Vermeer, Berry A Soute, Maastricht, Netherlands assigned to Rijksuniversiteit Liraburg Method for the extraction and insolubilization of a vitamin-K-dependent carboxylase complex from biological material, for instance a bovine liver homogenate, which can be used for the conversion of, for example, precursors of the blood coagulation factors II, VII, X and especially IX prepared via recombinant DNA-technotogy, the insolubilized carboxylase complex, as well as the prepared biologically active blood coagulation factors to be used in pharmaceutical compositions.
5101013
455
insulin and/or its derivatives of natural, semisynthetic or genetic engineering origin by ion exchanger chromatography on strongly acid cation exchangers.
5101024 HUMAN IMMUNOGLOBULIN AND ENHANCER GENE FRAGMENTS Akira Kudo, Satoshi Nakamura, Yoshihiko Sumi, Yataro Ichikawa, Takeshi Watanabe, Saga, Japan assigned to Teijin Limited The present invention provides an enhancer DNA segment, a gene fragment having (a) the enhancer DNA segment and (b) a structural gene such as human D, V and J gene, and a promoter. A hybrid DNA (1) having a phage DNA fragment from Charon 4A phage vector and human DNA fragments, a Charon 4A phage containing the hybrid DNA (1), a hybrid DNA (2) having a PBR322 fragment and human DNA fragment, a transformant of E. coil C600 strain transformed with the hybrid DNA (2), plasmid pSV2-H1GI obtained by inserting a human DNA fragment into a vector pSV-2gpt, a transformant of E. coli MC 1000 strain transformed with the plasmid pSV2-H1G1 and a transformant of mouse myeloma cells J558L or NS-1 transformed with the plasmid pSV2-HIG1 are also provided.
5104650 USES OF RECOMBINANT COLONY STIMULATING FACTOR-1
PROCESS FOR ISOLATING BASIC PROTEINS FROM PROTEIN MIXTURES
Peter Ralph, Mary K Warren, Kong T Chong, James J Devlin, Robert Zimmerman, Ada H C Kung assigned to Cetus Corporation
Michael Dorschug, Ranier Obermeier, Bochum, Federal Republic Of Germany assigned to Hoechst Aktiengesellschaft
A colony stimulating factor, CSF- 1, is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-I is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein.
Basic proteins are isolated from protein mixtures which contain such basic proteins and which are obtained by enzymatic clevage of pro-
5100794 AMYLOLYTIC ENZYMES PRODUCING MICROORGANISMS, CONSTRUCTED BY RECOMBINANT DNA TECHNOLOGY AND THEIR USE FOR PERMENTATION PROCESSES Alexander Strasser, Feodor B Martens, Jurge Dohmen, Cornelius P Hollenberg, Dusseldorf, Federal Republic Of Germany The cloning of Schwanniomyces glucoamytase and alpha-amylase genes is taught. The genes are expressed in recombinant host cells.
5100798 METHOD FOR THE EXTRACTION OF A VITAMIN-K-DEPENDENT CARBOXYLASE COMPLEX AND THE USE THEREOF Cornelis Vermeer, Berry A Soute, Maastricht, Netherlands assigned to Rijksuniversiteit Liraburg Method for the extraction and insolubilization of a vitamin-K-dependent carboxylase complex from biological material, for instance a bovine liver homogenate, which can be used for the conversion of, for example, precursors of the blood coagulation factors II, VII, X and especially IX prepared via recombinant DNA-technotogy, the insolubilized carboxylase complex, as well as the prepared biologically active blood coagulation factors to be used in pharmaceutical compositions.
5101013
455
insulin and/or its derivatives of natural, semisynthetic or genetic engineering origin by ion exchanger chromatography on strongly acid cation exchangers.
5101024 HUMAN IMMUNOGLOBULIN AND ENHANCER GENE FRAGMENTS Akira Kudo, Satoshi Nakamura, Yoshihiko Sumi, Yataro Ichikawa, Takeshi Watanabe, Saga, Japan assigned to Teijin Limited The present invention provides an enhancer DNA segment, a gene fragment having (a) the enhancer DNA segment and (b) a structural gene such as human D, V and J gene, and a promoter. A hybrid DNA (1) having a phage DNA fragment from Charon 4A phage vector and human DNA fragments, a Charon 4A phage containing the hybrid DNA (1), a hybrid DNA (2) having a PBR322 fragment and human DNA fragment, a transformant of E. coil C600 strain transformed with the hybrid DNA (2), plasmid pSV2-H1GI obtained by inserting a human DNA fragment into a vector pSV-2gpt, a transformant of E. coli MC 1000 strain transformed with the plasmid pSV2-H1G1 and a transformant of mouse myeloma cells J558L or NS-1 transformed with the plasmid pSV2-HIG1 are also provided.
5104650 USES OF RECOMBINANT COLONY STIMULATING FACTOR-1
PROCESS FOR ISOLATING BASIC PROTEINS FROM PROTEIN MIXTURES
Peter Ralph, Mary K Warren, Kong T Chong, James J Devlin, Robert Zimmerman, Ada H C Kung assigned to Cetus Corporation
Michael Dorschug, Ranier Obermeier, Bochum, Federal Republic Of Germany assigned to Hoechst Aktiengesellschaft
A colony stimulating factor, CSF- 1, is a lymphokine useful in overcoming the immunosuppression induced by chemotherapy or resulting from other causes. CSF-I is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein.
Basic proteins are isolated from protein mixtures which contain such basic proteins and which are obtained by enzymatic clevage of pro-