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5192675 Cloned KpnI restriction modification system
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PATENT ABSTRACTS
genetic analyses are disclosed herein. Also disclosed is a method for obtaining Variable Tandem Repeat polymorphism at a single genetic locus as well as other genetic analyses.
5192659 INTRON SEQUENCE ANALYSIS METHOD FOR DETECTION OF ADJACENT AND REMOTE LOCUS ALLELES AS HAPLOTYPES Malcolm J Simons, Fryerstown, Australia assigned to GeneType AG The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic D N A with a primer pair that spans an intron sequence and defines a D N A sequence in genetic linkage with an allele to be detected. The primer-defined D N A sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified DNA sequence characteristic of the allele. The amplified DNA sequence is analyzed to detect the presence of a genetic variation in the amplified D N A sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic of the allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described. 5192669 METHOD TO PRODUCE RECOMBINANT PROTEINS USING AN EXPRESSION VECTOR COMPRISING TRANSCRIPTIONAL AND TRANSLATIONAL ACTIVATING SEQUENCES Brigitte E Schoner, Ronald Schoner, Fryerstown assigned to Eli Lilly and Company The present invention is composed of novel recombinant D N A expression vectors which contain a transcriptional activating sequence, a translational activating sequence and a DNA sequence coding for a functional polypeptide,
especially bovine growth hormone. The aforementioned translational activating sequences contain a ribosome binding site and are designed to provide high level expression of DNA that codes for virtually any functional polypeptide. The invention further provides transformed microbial host cells capable of producing bovine growth hormone and other functional polypeptides at high levels. 51~6~ M U T A N T S T R A I N O F C. ACETOBUTYLICUM AND PROCESS FOR MAKING BUTANOL Mahendra Jain, Daniel Beacom, Rathin Datta assigned to Michigan Biotechnology Institute A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which as been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation process, and produces high concentrations of butanol and total solvents. 5192675 CLONED KPNI RESTRICTION MODIFICATION SYSTEM Deb K Chatterjee, Alan Hammond assigned to Life Technologies Inc The present invention discloses the cloning and expression in a host such as Escberichia coli of the KpnI restriction-modification system from Klebsiella pneumoniae, utilizing a two step protocol. Initial protection of the E. coli host with methylase expressed on a vector was required to stabilize a compatible vector carrying both the endonuclease and the methylase genes on a single DNA fragment. A chromosomal map was generated localizing the genes for Kpnl methylase and endonuclease. An E. coli strain was constructed which produced several thousand-fold higher levels of KpnI endonuclease than the level produced by Klebsiella pneumoniae. This invention is also directed to cloning and expression of genes encoding for restriction endonuclease isosehizomers of KpnI and/or modification methylase isosehizomers of KpnI methylase.
PATENT ABSTRACTS
genetic analyses are disclosed herein. Also disclosed is a method for obtaining Variable Tandem Repeat polymorphism at a single genetic locus as well as other genetic analyses.
5192659 INTRON SEQUENCE ANALYSIS METHOD FOR DETECTION OF ADJACENT AND REMOTE LOCUS ALLELES AS HAPLOTYPES Malcolm J Simons, Fryerstown, Australia assigned to GeneType AG The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic D N A with a primer pair that spans an intron sequence and defines a D N A sequence in genetic linkage with an allele to be detected. The primer-defined D N A sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified DNA sequence characteristic of the allele. The amplified DNA sequence is analyzed to detect the presence of a genetic variation in the amplified D N A sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic of the allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described. 5192669 METHOD TO PRODUCE RECOMBINANT PROTEINS USING AN EXPRESSION VECTOR COMPRISING TRANSCRIPTIONAL AND TRANSLATIONAL ACTIVATING SEQUENCES Brigitte E Schoner, Ronald Schoner, Fryerstown assigned to Eli Lilly and Company The present invention is composed of novel recombinant D N A expression vectors which contain a transcriptional activating sequence, a translational activating sequence and a DNA sequence coding for a functional polypeptide,
especially bovine growth hormone. The aforementioned translational activating sequences contain a ribosome binding site and are designed to provide high level expression of DNA that codes for virtually any functional polypeptide. The invention further provides transformed microbial host cells capable of producing bovine growth hormone and other functional polypeptides at high levels. 51~6~ M U T A N T S T R A I N O F C. ACETOBUTYLICUM AND PROCESS FOR MAKING BUTANOL Mahendra Jain, Daniel Beacom, Rathin Datta assigned to Michigan Biotechnology Institute A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which as been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation process, and produces high concentrations of butanol and total solvents. 5192675 CLONED KPNI RESTRICTION MODIFICATION SYSTEM Deb K Chatterjee, Alan Hammond assigned to Life Technologies Inc The present invention discloses the cloning and expression in a host such as Escberichia coli of the KpnI restriction-modification system from Klebsiella pneumoniae, utilizing a two step protocol. Initial protection of the E. coli host with methylase expressed on a vector was required to stabilize a compatible vector carrying both the endonuclease and the methylase genes on a single DNA fragment. A chromosomal map was generated localizing the genes for Kpnl methylase and endonuclease. An E. coli strain was constructed which produced several thousand-fold higher levels of KpnI endonuclease than the level produced by Klebsiella pneumoniae. This invention is also directed to cloning and expression of genes encoding for restriction endonuclease isosehizomers of KpnI and/or modification methylase isosehizomers of KpnI methylase.