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5354943 Methods of high frequency tissue regeneration, regeneration of herbicide-tolerant populus plants therewith, and the herbicide-tolerant plants made thereby
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PATENT ABSTRACTS
The invention relates to Proteobacteria that show unusually high level resistance to a wide range of metal oxides and oxyanions and to methods using selected subgroups for efficient reduction of certain metal oxides and oxyanions to the free metal. High level resistance was shown to be affected by growth conditions, and was observed in facultative photoheterotrophs such as Rhodobacter sphaeroides grown either chemoheterotrophically or photoheterotrophically.
5354555 VACCINE TO CONTROL THE VIRAL INFECTION OF FISH Leong Jo-Ann C Albany, OR, UNITED STATES Assigned to State of Oregon Acting By and Through The State Board of Higher Education on Behalf of Oregon State University Subunit vaccines and their use for immunizing fish against infection by viruses ate disclosed. In particular, plasmid pG8 is constructed by joining, with the plasmid pUC8, DNA which encodes the glycoprotein of infectious hematopoietic necrosis virus (IHNV). E. coli ceils are transformed by pGg, whereby pure viral antigen is produced to provide a vaccine for the control of IHNV in fish.
5354684 AGROBACTERIUM RADIOBACTER K84 CARRYING TRANSFER-DEFICIENT PAGK84 PLASMIDS Kerr Allen; Jones David A; Clare Bruce G; Ryder Maarten H;Farrand Stephen Adelaide, AUSTRALIA Assigned to Luminis Pry Lid The present invention provides transfer-deficient pAgK84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasmids include genes encoding the synthesis of the antibiotic agrocin g4 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop nonpathogenic strains of A. radiobaeter Kg4 that stably produce agrocin 84 and inhibit the development of crown gall disease.
5354943 METHODS OF HIGH FREQUENCY TISSUE REGENERATION, REGENERATION OF HERBICIDETOLERANT POPULUS PLANTS THEREWITH, AND THE HERBICIDE-TOLERANT PLANTS MADE THEREBY Michler Charles H; Haissig Bruce Rhinelander, WI, UNITED STATES Assigned to The United States of America as represented by the Secretary of the Agriculture Methods of in vitro culturing of tree tissue axe disclosed in which high frequency tissue regeneration is achieved thereby increasing the likelihood of regenerating somaclonal variants having desirable traits. These methods include: culturing with a ratio of naphthaleneacetic acid and benzyladenine, or of benzyladenine and zeatin, in a tissue regeneration medium, the ratio being selected to induce a high frequency of tissue regeneration; culturing on a tissue regeneration medium having a high auxin concentration with a relatively low cytokinin concentration for a short period of time, e.g. up to ten days, to regenerate a high number of morphogenic callus ceils; and, regenerating somatic embyros using a medium comprising 2,4-dichlorophenoxyacetic acid, benzyladenine, sucrose and glutamine, for high frequency regeneration of emboyogenic calli which are then developed into somatic embryos. These high frequency tissue regeneration methods axe used to develop plants having tolerance to toxic chemicals by combining the methods with in vitro exposure to toxic chemical thereby to challenge somaelonal variants with the toxic chemical to test for and recover tolerant variants.
5356623 BACILLUS THURINGIENSIS CRYET1 TOXIN GENE AND PROTEIN TOXIC TO LEPIDOPTERAN INSECTS yon Tersch Michael A; Gonzalez Jose M Ewing Township, NJ, UNITED STATES Assigned to Ecogen lnc A Bacillus thuringiensis strain isolate, designated EG5092, exhibits insecticidal activity against
PATENT ABSTRACTS
The invention relates to Proteobacteria that show unusually high level resistance to a wide range of metal oxides and oxyanions and to methods using selected subgroups for efficient reduction of certain metal oxides and oxyanions to the free metal. High level resistance was shown to be affected by growth conditions, and was observed in facultative photoheterotrophs such as Rhodobacter sphaeroides grown either chemoheterotrophically or photoheterotrophically.
5354555 VACCINE TO CONTROL THE VIRAL INFECTION OF FISH Leong Jo-Ann C Albany, OR, UNITED STATES Assigned to State of Oregon Acting By and Through The State Board of Higher Education on Behalf of Oregon State University Subunit vaccines and their use for immunizing fish against infection by viruses ate disclosed. In particular, plasmid pG8 is constructed by joining, with the plasmid pUC8, DNA which encodes the glycoprotein of infectious hematopoietic necrosis virus (IHNV). E. coli ceils are transformed by pGg, whereby pure viral antigen is produced to provide a vaccine for the control of IHNV in fish.
5354684 AGROBACTERIUM RADIOBACTER K84 CARRYING TRANSFER-DEFICIENT PAGK84 PLASMIDS Kerr Allen; Jones David A; Clare Bruce G; Ryder Maarten H;Farrand Stephen Adelaide, AUSTRALIA Assigned to Luminis Pry Lid The present invention provides transfer-deficient pAgK84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasmids include genes encoding the synthesis of the antibiotic agrocin g4 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop nonpathogenic strains of A. radiobaeter Kg4 that stably produce agrocin 84 and inhibit the development of crown gall disease.
5354943 METHODS OF HIGH FREQUENCY TISSUE REGENERATION, REGENERATION OF HERBICIDETOLERANT POPULUS PLANTS THEREWITH, AND THE HERBICIDE-TOLERANT PLANTS MADE THEREBY Michler Charles H; Haissig Bruce Rhinelander, WI, UNITED STATES Assigned to The United States of America as represented by the Secretary of the Agriculture Methods of in vitro culturing of tree tissue axe disclosed in which high frequency tissue regeneration is achieved thereby increasing the likelihood of regenerating somaclonal variants having desirable traits. These methods include: culturing with a ratio of naphthaleneacetic acid and benzyladenine, or of benzyladenine and zeatin, in a tissue regeneration medium, the ratio being selected to induce a high frequency of tissue regeneration; culturing on a tissue regeneration medium having a high auxin concentration with a relatively low cytokinin concentration for a short period of time, e.g. up to ten days, to regenerate a high number of morphogenic callus ceils; and, regenerating somatic embyros using a medium comprising 2,4-dichlorophenoxyacetic acid, benzyladenine, sucrose and glutamine, for high frequency regeneration of emboyogenic calli which are then developed into somatic embryos. These high frequency tissue regeneration methods axe used to develop plants having tolerance to toxic chemicals by combining the methods with in vitro exposure to toxic chemical thereby to challenge somaelonal variants with the toxic chemical to test for and recover tolerant variants.
5356623 BACILLUS THURINGIENSIS CRYET1 TOXIN GENE AND PROTEIN TOXIC TO LEPIDOPTERAN INSECTS yon Tersch Michael A; Gonzalez Jose M Ewing Township, NJ, UNITED STATES Assigned to Ecogen lnc A Bacillus thuringiensis strain isolate, designated EG5092, exhibits insecticidal activity against