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5354943 Methods of high frequency tissue regeneration, regeneration of herbicide-tolerant populus plants therewith, and the herbicide-tolerant plants m
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PATENT ABSTRACTS
such as Rhodobacter sphaeroides grown either chemoheterotrophically or photoheterotrophically.
5354555 VACCINE TO CONTROL THE VIRAL INFECTION OF FISH Jo-Ann C Leong assigned to State of Oregon Acting By and Through The State Board of Higher Education on Behalf of Oregon State University Subunit vaccines and their use for immuni~6ng fish against infection by viruses are disclosed. In particular, plasmid pG8 is constructed by joining, with the plasmid pUC8, DNA which encodes the glycoprotein of infectious hematopoietic necrosis virus (IHNV). E. coil cells are transformed by pGS, whereby pu~ viral antigen is produced to provide a vaccine for the control of IHNV in fish.
5354684 AGROBACTERIUM RADIOBACTER K84 CARRYING TRANSFER-DEFICIENT PAGK84 PLASMIDS Allen Kerr, David A Jones, Bruce G Clare, Maarten H Ryder, Stephen Farrand, Adelaide, Australia assigned to Luminis Pry Ltd The present invention provides transfcrdeficient pA~K84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasndds include genes encoding the synthesis of the antibiotic agrocin 84 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop nonpathogenic strains of A. radiobacter K84 that stably produce agrocin 84 and inhibit the development of crown gall disease.
5354943 METHODS OF HIGH FREQUENCY TISSUE REGENERATION, REGENERATION OF HERBICIDE-TOLERANT POPULUS PLANTS THEREWITH, AND THE HERBICIDE-TOLERANT PLANTS MADE THEREBY Charles H Michler, Bruce Haissig assigned to The United States of America as represented by the Secretary of the Agriculture Methods of in vitro culturing of tree tissue are disclosed in which high frequency tissue regeneration is achieved thereby increasing the likefihood of regenerating somaclonal variants having desirable traits. These methods include: culturing with a ratio of naphthaleneacetic acid and benzyladenine, or of benzyladeniue and zeatin, in a tissue regeneration medium, the ratio being selected to induce a high frequency of tissue regeneration; culturing on a tissue regeneration medium having a high auxin concentration with a relatively low cytokinin concentration for a short period of time, e.g. up to ten days, to regenerate a high number of morphogenic callus cells; and, regenerating somatic embyros using a medium comprising 2,4-dichlorophenoxyacetic acid, benzyladenine, sucrose and glutamiue, for high frequency regeneration of emboyogenic calli which are then developed into somatic embryos. These high frequency tissue regeneration methods are used to develop plants having tolerance to toxic chemicals by combining the methods with in vitro exposure to toxic chemical thereby to challenge somaclonal variants with the toxic chemical to test for and recover tolerant variants.
5356623 BACILLUS THURINGIENSIS CRYET1 TOXIN GENE AND PROTEIN TOXIC TO LEPIDOPTERAN INSECTS Michael A v o n Tersch, Jose M G-onzalez assigned to Ecogen Inc A Bacillus thuringiensis strain isolate, designated EG5092, exhibits insecticidal activity against lepidopteran insects. A purified and isolated novel cryET1 toxin geneproduct from B.t. strain EG5092 exhibits specific insecticidal activity against Plutella xylostella (Diamond-
PATENT ABSTRACTS
such as Rhodobacter sphaeroides grown either chemoheterotrophically or photoheterotrophically.
5354555 VACCINE TO CONTROL THE VIRAL INFECTION OF FISH Jo-Ann C Leong assigned to State of Oregon Acting By and Through The State Board of Higher Education on Behalf of Oregon State University Subunit vaccines and their use for immuni~6ng fish against infection by viruses are disclosed. In particular, plasmid pG8 is constructed by joining, with the plasmid pUC8, DNA which encodes the glycoprotein of infectious hematopoietic necrosis virus (IHNV). E. coil cells are transformed by pGS, whereby pu~ viral antigen is produced to provide a vaccine for the control of IHNV in fish.
5354684 AGROBACTERIUM RADIOBACTER K84 CARRYING TRANSFER-DEFICIENT PAGK84 PLASMIDS Allen Kerr, David A Jones, Bruce G Clare, Maarten H Ryder, Stephen Farrand, Adelaide, Australia assigned to Luminis Pry Ltd The present invention provides transfcrdeficient pA~K84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasndds include genes encoding the synthesis of the antibiotic agrocin 84 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop nonpathogenic strains of A. radiobacter K84 that stably produce agrocin 84 and inhibit the development of crown gall disease.
5354943 METHODS OF HIGH FREQUENCY TISSUE REGENERATION, REGENERATION OF HERBICIDE-TOLERANT POPULUS PLANTS THEREWITH, AND THE HERBICIDE-TOLERANT PLANTS MADE THEREBY Charles H Michler, Bruce Haissig assigned to The United States of America as represented by the Secretary of the Agriculture Methods of in vitro culturing of tree tissue are disclosed in which high frequency tissue regeneration is achieved thereby increasing the likefihood of regenerating somaclonal variants having desirable traits. These methods include: culturing with a ratio of naphthaleneacetic acid and benzyladenine, or of benzyladeniue and zeatin, in a tissue regeneration medium, the ratio being selected to induce a high frequency of tissue regeneration; culturing on a tissue regeneration medium having a high auxin concentration with a relatively low cytokinin concentration for a short period of time, e.g. up to ten days, to regenerate a high number of morphogenic callus cells; and, regenerating somatic embyros using a medium comprising 2,4-dichlorophenoxyacetic acid, benzyladenine, sucrose and glutamiue, for high frequency regeneration of emboyogenic calli which are then developed into somatic embryos. These high frequency tissue regeneration methods are used to develop plants having tolerance to toxic chemicals by combining the methods with in vitro exposure to toxic chemical thereby to challenge somaclonal variants with the toxic chemical to test for and recover tolerant variants.
5356623 BACILLUS THURINGIENSIS CRYET1 TOXIN GENE AND PROTEIN TOXIC TO LEPIDOPTERAN INSECTS Michael A v o n Tersch, Jose M G-onzalez assigned to Ecogen Inc A Bacillus thuringiensis strain isolate, designated EG5092, exhibits insecticidal activity against lepidopteran insects. A purified and isolated novel cryET1 toxin geneproduct from B.t. strain EG5092 exhibits specific insecticidal activity against Plutella xylostella (Diamond-