- Email: [email protected]
5366891 Biochemical solubilization of toxic salts from residual geothermal brines and waste waters
87
PATENT ABSTRACTS Through use of the present method, site-directed cleavage of an RNA molecule is effected, followed by excision of the selected or target segment of the RNA molecule.
5366882 M E T H O D FOR P R O D U C I N G THE BGLI RESTRICTION ENDONUCLEASE AND METHYLASE
bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi TLDNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lecfin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.
Lunnen Keith; Wilson Geoffrey - New England Biolabs In accordance with the present invention there is provided an isolated DNA coding for the Bgfl restriction endonuclease and modification methylase derived from Bacillus globigii RUB561 stain, as well as related methods for cloning said recombinant DNA. The present invention also relates to clones which express recombinant Bg/I restriction endonuclease and recombinant modification methylase produced from Bg/I recombinant DNA and to methods for producing said enzymes.
5366883 A L P H A - A M Y L A S E GENE Asada Kiyozo; Uemori Takash; Mukai Hiroyuk; Kato Ikunoshi; Laderman Kenneth; Anfinsen Christian B - Takara Shuzo Co Ltd; The Johns Hopkins Universi The present invention relates, in general, to a cloned alpha-amylase gene, and, in particular, to a cloned hyperthermophilic alpha-amylase gene and to methods of producing alpha-amylase using same.
5366887 RI T-DNA P R O M O T E R S
5366889 DNA E N C O D I N G A PROTEINC O U P L E D R E C E P T O R KINASE MacDonald Marcy E; Gusella James; Ambrose Christine - The General Hospital Corporation This invention relates to a DNA segment which encodes at least one member of the family of mammalian G protein-coupled receptor kinases, which maps to the Huntington's disease region within the terminal cytogenic subband of the short arm of chromosome 4, 4p16.3, between DNA marker D4S10 and the telomere region. Further, this invention provides the substantially purified expression product of a gene from the Huntington's disease region of the chromosome, as well as related diagnostic and therapeutic uses.
5366891 BIOCHEMICAL S O L U B I L I Z A T I O N OF TOXIC SALTS F R O M R E S I D U A L G E O T H E R M A L BRINES AND W A S T E WATERS
Slightom Jerry L; Tepfer David, Kalamazoo, MI, UNITED STATES - Mycogen Plant Sciences Inc
Premuzic Eugene; Lin Mow S~ East Moriches, NY, UNITED STATES - Associated Universities Inc
The sequence of the TL-DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames
A method of solubilizing metal salts such as metal sulfides in a geothermal sludge using mutant Thiobacilli selected for their ability to metabolize
88
PATENT ABSTRACTS
metal sails at high temperature is disclosed, The method includes the introduction of mutated Thiobacillus ferrooxidans and Thiobacillus thiooxidans to a geothermal sludge or brine. The microorganisms catalyze the solubilization of metal salts, For instance, in the case of metal sulfides, the microorganisms catalyze the solubilization to form soluble metal sulfates.
5366892 GENE ENCODING A C O L E O P T E R A N - A C T I V E TOXIN Foncerrada Luis; Sick August J; Payne Jewel M Mycogen Corporation The subject invention concerns a novel microbe and gene encoding a novel toxin protein with activity against insect pests of the order Coleoptera. Pests in the order Coleoptera do heavy damage to crops, e.g.,corn. The novel Bacillus thuringiensis microbe of the invention is referred to as B.t. PSSOC. The spores or crystals of this microbe, or mutants thereof, are useful to control coleopteran pests in various environments. The novel gene of the invention can be used to transform various hosts wherein the novel toxic protein can be expressed.
5366894 PEPTIDE PRODUCTION Clark Anthony; Lathe Richard, Edinburgh, UNITED KINGDOM - Pharmaceutical Proteins Limited A method of producing a substance comprising a peptide, involves incorporating a DNA sequence coding for the peptide into a gene of a mammal (such as a sheep) coding for a milk whey protein in such a way that the DNA sequence is expressed in the mammary gland of the adult female mammal. The substance may be an (optionally modified) protein such as a blood coagulation factor. The DNA sequence is preferably inserted into the first exon of a gene coding for a whey protein such as beta-lactoglobulin. The substance will generally be recovered from milk of the female mammal, but may (for example if it is an enzyme) be used in situ.
5367052 AMYLIN PEPTIDES Cooper Garth J S; Willis Antony C, Solana Beach, CA, UNITED STATES - Amylin Pharmaceuticals Inc A biologically active peptide associated with diabetes and designated herein amylin and processes for preparing it and assaying for it and for Type 2 diabetes are disclosed. The invention includes peptides having the amino acid sequence KCNTATCATQRLANFLVHSSNNFGAILSSTN VGSNTY, substantially homologous sequences of amino acids, proamylin, as well as biologically active subfragments and conservative mutations. The peptide may be prepared from diabetic pancreata by solubilization of amyloid and isolation of the peptide by gel filtration and reverse phase chromotography. Amylin may also be synthesized, or it may be produced by recombinant DNA techniques using the disclosed nucleic acid sequences.
5367059 CYS-SER-VAL-THR-CYS-GLY SPECIFIC T U M O R CELL ADHESION RECEPTOR Tuszynski George P; Eyal Jacob; Hamilton Bruce W R Grace & Co -Conn; The Medical College of The present invention provides a cell matrix receptor specific for the Cys Ser Val Thr Cys Gly (SEQ ID NO: 1) region of thrombospondin. Also provided are purification, cloning and expression methods. Antibodies directed against the receptor protein are useful in numerous diagnostic, prophylactic and therapeutic areas.
5367060 STRUCTURE, PRODUCTION AND USE OF HEREGULIN Vandlen Richard Genentech lnc
L;
Holmes
William
E
-
A novel polypeptide with binding affinity for the p185HER2 receptor, designated heregulin- alpha,
PATENT ABSTRACTS Through use of the present method, site-directed cleavage of an RNA molecule is effected, followed by excision of the selected or target segment of the RNA molecule.
5366882 M E T H O D FOR P R O D U C I N G THE BGLI RESTRICTION ENDONUCLEASE AND METHYLASE
bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi TLDNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lecfin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.
Lunnen Keith; Wilson Geoffrey - New England Biolabs In accordance with the present invention there is provided an isolated DNA coding for the Bgfl restriction endonuclease and modification methylase derived from Bacillus globigii RUB561 stain, as well as related methods for cloning said recombinant DNA. The present invention also relates to clones which express recombinant Bg/I restriction endonuclease and recombinant modification methylase produced from Bg/I recombinant DNA and to methods for producing said enzymes.
5366883 A L P H A - A M Y L A S E GENE Asada Kiyozo; Uemori Takash; Mukai Hiroyuk; Kato Ikunoshi; Laderman Kenneth; Anfinsen Christian B - Takara Shuzo Co Ltd; The Johns Hopkins Universi The present invention relates, in general, to a cloned alpha-amylase gene, and, in particular, to a cloned hyperthermophilic alpha-amylase gene and to methods of producing alpha-amylase using same.
5366887 RI T-DNA P R O M O T E R S
5366889 DNA E N C O D I N G A PROTEINC O U P L E D R E C E P T O R KINASE MacDonald Marcy E; Gusella James; Ambrose Christine - The General Hospital Corporation This invention relates to a DNA segment which encodes at least one member of the family of mammalian G protein-coupled receptor kinases, which maps to the Huntington's disease region within the terminal cytogenic subband of the short arm of chromosome 4, 4p16.3, between DNA marker D4S10 and the telomere region. Further, this invention provides the substantially purified expression product of a gene from the Huntington's disease region of the chromosome, as well as related diagnostic and therapeutic uses.
5366891 BIOCHEMICAL S O L U B I L I Z A T I O N OF TOXIC SALTS F R O M R E S I D U A L G E O T H E R M A L BRINES AND W A S T E WATERS
Slightom Jerry L; Tepfer David, Kalamazoo, MI, UNITED STATES - Mycogen Plant Sciences Inc
Premuzic Eugene; Lin Mow S~ East Moriches, NY, UNITED STATES - Associated Universities Inc
The sequence of the TL-DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames
A method of solubilizing metal salts such as metal sulfides in a geothermal sludge using mutant Thiobacilli selected for their ability to metabolize
88
PATENT ABSTRACTS
metal sails at high temperature is disclosed, The method includes the introduction of mutated Thiobacillus ferrooxidans and Thiobacillus thiooxidans to a geothermal sludge or brine. The microorganisms catalyze the solubilization of metal salts, For instance, in the case of metal sulfides, the microorganisms catalyze the solubilization to form soluble metal sulfates.
5366892 GENE ENCODING A C O L E O P T E R A N - A C T I V E TOXIN Foncerrada Luis; Sick August J; Payne Jewel M Mycogen Corporation The subject invention concerns a novel microbe and gene encoding a novel toxin protein with activity against insect pests of the order Coleoptera. Pests in the order Coleoptera do heavy damage to crops, e.g.,corn. The novel Bacillus thuringiensis microbe of the invention is referred to as B.t. PSSOC. The spores or crystals of this microbe, or mutants thereof, are useful to control coleopteran pests in various environments. The novel gene of the invention can be used to transform various hosts wherein the novel toxic protein can be expressed.
5366894 PEPTIDE PRODUCTION Clark Anthony; Lathe Richard, Edinburgh, UNITED KINGDOM - Pharmaceutical Proteins Limited A method of producing a substance comprising a peptide, involves incorporating a DNA sequence coding for the peptide into a gene of a mammal (such as a sheep) coding for a milk whey protein in such a way that the DNA sequence is expressed in the mammary gland of the adult female mammal. The substance may be an (optionally modified) protein such as a blood coagulation factor. The DNA sequence is preferably inserted into the first exon of a gene coding for a whey protein such as beta-lactoglobulin. The substance will generally be recovered from milk of the female mammal, but may (for example if it is an enzyme) be used in situ.
5367052 AMYLIN PEPTIDES Cooper Garth J S; Willis Antony C, Solana Beach, CA, UNITED STATES - Amylin Pharmaceuticals Inc A biologically active peptide associated with diabetes and designated herein amylin and processes for preparing it and assaying for it and for Type 2 diabetes are disclosed. The invention includes peptides having the amino acid sequence KCNTATCATQRLANFLVHSSNNFGAILSSTN VGSNTY, substantially homologous sequences of amino acids, proamylin, as well as biologically active subfragments and conservative mutations. The peptide may be prepared from diabetic pancreata by solubilization of amyloid and isolation of the peptide by gel filtration and reverse phase chromotography. Amylin may also be synthesized, or it may be produced by recombinant DNA techniques using the disclosed nucleic acid sequences.
5367059 CYS-SER-VAL-THR-CYS-GLY SPECIFIC T U M O R CELL ADHESION RECEPTOR Tuszynski George P; Eyal Jacob; Hamilton Bruce W R Grace & Co -Conn; The Medical College of The present invention provides a cell matrix receptor specific for the Cys Ser Val Thr Cys Gly (SEQ ID NO: 1) region of thrombospondin. Also provided are purification, cloning and expression methods. Antibodies directed against the receptor protein are useful in numerous diagnostic, prophylactic and therapeutic areas.
5367060 STRUCTURE, PRODUCTION AND USE OF HEREGULIN Vandlen Richard Genentech lnc
L;
Holmes
William
E
-
A novel polypeptide with binding affinity for the p185HER2 receptor, designated heregulin- alpha,