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5401632 Triple helix purification and sequencing
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PATENT ABSTRACTS
5401630 SPECIES.SPECIFIC DNA-DNA HYBRIDIZATION PROBE PREPARED USING CHROMOSOME SIZE DNA Torok Tama; King A Douglas: Rockhold David R; Royer Christin Albany, CA, UNITED STATES Assigned to The United States of America as represented by the Secretary of Agriculture
A species-specific DNA-DNA hybridization probe prepared using an isolated whole chromosome of a lower eukaryote as template. The probe is useful for the detection of conspecificity in lower eukaryotes selected from the group consisting of yeast and molds.
5401632 TRIPLE HELIX PURIFICATION AND SEQUENCING Wang Renfeng; Smith Lloyd M; Tong Xinchun Dublin, CA, UNITED STATES Assigned to Wisconsin Alumni Research Foundation Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.
5401635 NUCLEIC ACIDS ENCODING HUMAN PROHIBITIN MUTANTS AND DETECTION THEREOF Nakamura Yusuk; Sato Takaaki Tokyo, JAPAN Assigned to Cancer Institute; Eisai Co Ltd
A human prohibitin gene, a protein coded for by said gene, a gene analysis reagent to be used with them, and a quantitative determination of prohibitin in a biological sample by an immunological technique with the use of an antihuman prohibitin antibody and a method for analyzing a prohibitin gene of a human tissue for the occurrence of mutation by the PCR method with the use of oligonucleotides having partial base sequences of said gene as primers.
5401638 DETECTION AND QUANTIFICATION OF NEU RELATED PROTEINS IN THE BIOLOGICAL FLUIDS OF HUMANS Carney Walter P; McKenzie Sara J North Andover, MA, UNITED STATES Assigned to Oncogene Science Inc This invention relates to a substantially purified pl00 which is a human neu related protein having a molecular weight in the range from about 97,000 daltons to about 115,000 daltons which corresponds substantially to the extracelhilar domain of the human neu gene product, said protein being detectable in a biological fluid. In another embodiment this invention relates to assays for detecting this protein. Finally, this invention also concerns monoelonal antibodies which are capable of binding to pl00.
5401642 VECTORS AND METHODS FOR MAKING SUCH VECTORS AND FOR EXPRESSING CLONED GENES Fiers Walter C; Remaut Erik R Destelbergen, BELGIUM Assigned to Biogen Inc Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from lambda phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are
PATENT ABSTRACTS
5401630 SPECIES.SPECIFIC DNA-DNA HYBRIDIZATION PROBE PREPARED USING CHROMOSOME SIZE DNA Torok Tama; King A Douglas: Rockhold David R; Royer Christin Albany, CA, UNITED STATES Assigned to The United States of America as represented by the Secretary of Agriculture
A species-specific DNA-DNA hybridization probe prepared using an isolated whole chromosome of a lower eukaryote as template. The probe is useful for the detection of conspecificity in lower eukaryotes selected from the group consisting of yeast and molds.
5401632 TRIPLE HELIX PURIFICATION AND SEQUENCING Wang Renfeng; Smith Lloyd M; Tong Xinchun Dublin, CA, UNITED STATES Assigned to Wisconsin Alumni Research Foundation Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.
5401635 NUCLEIC ACIDS ENCODING HUMAN PROHIBITIN MUTANTS AND DETECTION THEREOF Nakamura Yusuk; Sato Takaaki Tokyo, JAPAN Assigned to Cancer Institute; Eisai Co Ltd
A human prohibitin gene, a protein coded for by said gene, a gene analysis reagent to be used with them, and a quantitative determination of prohibitin in a biological sample by an immunological technique with the use of an antihuman prohibitin antibody and a method for analyzing a prohibitin gene of a human tissue for the occurrence of mutation by the PCR method with the use of oligonucleotides having partial base sequences of said gene as primers.
5401638 DETECTION AND QUANTIFICATION OF NEU RELATED PROTEINS IN THE BIOLOGICAL FLUIDS OF HUMANS Carney Walter P; McKenzie Sara J North Andover, MA, UNITED STATES Assigned to Oncogene Science Inc This invention relates to a substantially purified pl00 which is a human neu related protein having a molecular weight in the range from about 97,000 daltons to about 115,000 daltons which corresponds substantially to the extracelhilar domain of the human neu gene product, said protein being detectable in a biological fluid. In another embodiment this invention relates to assays for detecting this protein. Finally, this invention also concerns monoelonal antibodies which are capable of binding to pl00.
5401642 VECTORS AND METHODS FOR MAKING SUCH VECTORS AND FOR EXPRESSING CLONED GENES Fiers Walter C; Remaut Erik R Destelbergen, BELGIUM Assigned to Biogen Inc Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from lambda phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site for cloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are