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5595871 Detection and prevention of mycoplasma hominis infection
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PATENT ABSTRACTS
The sequence of the protein coding regions of the bcl-2 gene are provided as well as bacterial clones which produce the proteins. Assays are provided for detecting a class of B-cell neoplasms associated with a chromosome translocation between chromosomes 14 and 18. This translocation is involved in the majority of cases of human follicular lymphomas. One assay employs an antibody which is immunoreactive with a human protein which is over-expressed due to the chromosome translocation. Another assay involves measurement of the amount of mRNA which hybridizes to the gene proximal to the translocation break-point.
5595870 IDENTIFYING NUCLEIC ACIDS BY RESTRICTION DIGESTION A N D H Y B R I D I Z A T I O N WITH R A N D O M OR P S E U D O R A N D O M OLIGONUCLEOTIDES Knapp Michael R; Goelet Philip New York, NY, UNITED STATES Assigned to The Trustees of Columbia University in the City of New York This invention provides a method of determining whether a nucleic acid molecule having a sequence of interest is present within a nucleic acid-containing sample which comprises the steps: (A) subjecting said nucleic acid of said sample to a treatment sufficient to fragment said nucleic acid and to produce a plurality of fragments derived from said nucleic acid; (B) fractionating said plurality of fragments; (C) contacting said fractionated plurality of fragments, under hybridizing conditions, with a reagent comprising a mixture of random or pseudorandom oligonucleotides, said oligonucleotides having a length a > or =3 and < or =1000 nucleotides; (D) detecting the pattern of hybrids that form from said contacting of said fractionated nucleic acid molecules and said oligonucleotides of the mixture of random or pseudorandom oligonucleotides; and (E) determining whether said nucleic acid molecule having said sequence of interest is present in said sample by determining whether said pattern is indicative of the presence of said molecule. This invention provides a method of obtaining a signature for a nucleic acid of interest and for constructing a restriction map of a nucleic acid of
interest. This invention finally provides a method of identifying a nucleic acid sequence which encodes, directly or indirectly, a particular biological activity of interest.
5595871 DETECTION A N D PREVENTION OF M Y C O P L A S M A HOMINIS INFECTION DelVecchio Vito G; Gallia Gary; McCieskey Ferne K Scranton, PA, UNITED STATES Assigned to University of Scranton; Research Corporation Technologies Inc The present invention is directed to a rapid and sensitive method for detecting Mycoplasma hominis using M. hominis-specific probes, oligonucleotides or antibodies. In particular a target sequence can be arnplified by in vitro nucleic acid amplification techniques, detected by nucleic acid hybridization using the subject probes and oligonucleotides or detected by immunoassay using M. hominis-specific antibodies. M. hominis-specific nucleic acids which do not recognize or hybridize to genomic nucleic acid of other Mycoplasma species are also provided.
5595872 NUCLEIC ACIDS ENCODING M I C R O S O M A L TRIGYCERIDE TRANSFER PROTEIN Wetterau John; Sharp Daru Y; Gregg Richard E; Biller Scott A; Dickson John K; Lawrence R Michael; Lawson John E; Holava Henry M; Partyka Richard A Langhorne, PA, UNITED STATES Assigned to Bristol-Myers Squibb Company Nucleic acid sequences, particularly DNA sequences, coding for all or part of the high molecular weight subunit of microsomal triglyceride transfer protein, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of the high molecular weight subunit of
PATENT ABSTRACTS
The sequence of the protein coding regions of the bcl-2 gene are provided as well as bacterial clones which produce the proteins. Assays are provided for detecting a class of B-cell neoplasms associated with a chromosome translocation between chromosomes 14 and 18. This translocation is involved in the majority of cases of human follicular lymphomas. One assay employs an antibody which is immunoreactive with a human protein which is over-expressed due to the chromosome translocation. Another assay involves measurement of the amount of mRNA which hybridizes to the gene proximal to the translocation break-point.
5595870 IDENTIFYING NUCLEIC ACIDS BY RESTRICTION DIGESTION A N D H Y B R I D I Z A T I O N WITH R A N D O M OR P S E U D O R A N D O M OLIGONUCLEOTIDES Knapp Michael R; Goelet Philip New York, NY, UNITED STATES Assigned to The Trustees of Columbia University in the City of New York This invention provides a method of determining whether a nucleic acid molecule having a sequence of interest is present within a nucleic acid-containing sample which comprises the steps: (A) subjecting said nucleic acid of said sample to a treatment sufficient to fragment said nucleic acid and to produce a plurality of fragments derived from said nucleic acid; (B) fractionating said plurality of fragments; (C) contacting said fractionated plurality of fragments, under hybridizing conditions, with a reagent comprising a mixture of random or pseudorandom oligonucleotides, said oligonucleotides having a length a > or =3 and < or =1000 nucleotides; (D) detecting the pattern of hybrids that form from said contacting of said fractionated nucleic acid molecules and said oligonucleotides of the mixture of random or pseudorandom oligonucleotides; and (E) determining whether said nucleic acid molecule having said sequence of interest is present in said sample by determining whether said pattern is indicative of the presence of said molecule. This invention provides a method of obtaining a signature for a nucleic acid of interest and for constructing a restriction map of a nucleic acid of
interest. This invention finally provides a method of identifying a nucleic acid sequence which encodes, directly or indirectly, a particular biological activity of interest.
5595871 DETECTION A N D PREVENTION OF M Y C O P L A S M A HOMINIS INFECTION DelVecchio Vito G; Gallia Gary; McCieskey Ferne K Scranton, PA, UNITED STATES Assigned to University of Scranton; Research Corporation Technologies Inc The present invention is directed to a rapid and sensitive method for detecting Mycoplasma hominis using M. hominis-specific probes, oligonucleotides or antibodies. In particular a target sequence can be arnplified by in vitro nucleic acid amplification techniques, detected by nucleic acid hybridization using the subject probes and oligonucleotides or detected by immunoassay using M. hominis-specific antibodies. M. hominis-specific nucleic acids which do not recognize or hybridize to genomic nucleic acid of other Mycoplasma species are also provided.
5595872 NUCLEIC ACIDS ENCODING M I C R O S O M A L TRIGYCERIDE TRANSFER PROTEIN Wetterau John; Sharp Daru Y; Gregg Richard E; Biller Scott A; Dickson John K; Lawrence R Michael; Lawson John E; Holava Henry M; Partyka Richard A Langhorne, PA, UNITED STATES Assigned to Bristol-Myers Squibb Company Nucleic acid sequences, particularly DNA sequences, coding for all or part of the high molecular weight subunit of microsomal triglyceride transfer protein, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of the high molecular weight subunit of