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A copper detoxification complex and a copper metalloregulatory protein from an E. Coli copper resistance system
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Abstrmts
MO15
A COPPER DETOXIFICATION COMPLEX AND A COPPER METALLOFKEGULATORY PROTEIN FROM AN E. COLZ COPPER RESISTANCE SYSTEM
es W. Brvsona, Diana M. Ralstonb, Thomas V. O’Hall~ran~~ bDepartment of Biochemistry, aDepartment of Chemistry; Molecular Biology, Cell Biology; Northwestern University, Evanston, IL 60208 USA We have observed a soluble -5kD copper complex in the culture supernatants of the copper resistant E. coli strain ED8739/pRJ1004, suggesting that this plasmid-based copper resistance system involves cellular modification of the extracellular soluble copper pool. This result is consistent with predictions made from genetic studies that the pco plasmidencoded resistance operates by enhanced effl ux and Purification and initial modification of copper. I characterization of the copper detoxification complex will be presented. The genes responsible for this copper resistance mechanism are inducible by sub-toxic concentrations of copper, suggesting the presence of copper responsive regulatory proteins. Genetic evidence suggests that the PcoR gene one component of the regulatory apparatus.2 Overproduction and purification of the plasmid-encoded metalloregulatory protein PcoR will be described. PcoR’s ability to regulate transcription in response to changes in copper concentration is being investigated by in vitro run-off transcription reactions and gel shift mobility assays. Initial characterization of PcoR and it’s ability to bind copper and DNA will be presented. 1. D. Rouch, B. T. 0. Lee and J. Camakaris, in Metal Ion Homeostasis: Molecular Biology and Chemistry, UCLA Symposium on Molecular and Cellular Biology, New Series, D. Winge, D. Warner, Eds., Alan R. Liss Inc., New York, 1989, Vol. 98, p. 449. 2. D. Rouch, J. Camakaris, B. T. 0. Lee and R. K. J. Luke, J. Gen. Microbial. 131,939 (1985).
Abstrmts
MO15
A COPPER DETOXIFICATION COMPLEX AND A COPPER METALLOFKEGULATORY PROTEIN FROM AN E. COLZ COPPER RESISTANCE SYSTEM
es W. Brvsona, Diana M. Ralstonb, Thomas V. O’Hall~ran~~ bDepartment of Biochemistry, aDepartment of Chemistry; Molecular Biology, Cell Biology; Northwestern University, Evanston, IL 60208 USA We have observed a soluble -5kD copper complex in the culture supernatants of the copper resistant E. coli strain ED8739/pRJ1004, suggesting that this plasmid-based copper resistance system involves cellular modification of the extracellular soluble copper pool. This result is consistent with predictions made from genetic studies that the pco plasmidencoded resistance operates by enhanced effl ux and Purification and initial modification of copper. I characterization of the copper detoxification complex will be presented. The genes responsible for this copper resistance mechanism are inducible by sub-toxic concentrations of copper, suggesting the presence of copper responsive regulatory proteins. Genetic evidence suggests that the PcoR gene one component of the regulatory apparatus.2 Overproduction and purification of the plasmid-encoded metalloregulatory protein PcoR will be described. PcoR’s ability to regulate transcription in response to changes in copper concentration is being investigated by in vitro run-off transcription reactions and gel shift mobility assays. Initial characterization of PcoR and it’s ability to bind copper and DNA will be presented. 1. D. Rouch, B. T. 0. Lee and J. Camakaris, in Metal Ion Homeostasis: Molecular Biology and Chemistry, UCLA Symposium on Molecular and Cellular Biology, New Series, D. Winge, D. Warner, Eds., Alan R. Liss Inc., New York, 1989, Vol. 98, p. 449. 2. D. Rouch, J. Camakaris, B. T. 0. Lee and R. K. J. Luke, J. Gen. Microbial. 131,939 (1985).