- Email: [email protected]
A novel model of pig-to-monkey kidney xenotransplantation
A Novel Model of Pig-to-Monkey Kidney Xenotransplantation X.M. Wang, G. Chen, S. Chen, S.Q. Shen, T. Zhu, H. Wang, Y. Wu, L.J. Chen, and J.G. Zhu
I
N the last few years, important progress in xenotransplantation has been stimulated by a shortage of organs available for clinical use. Xenotransplantation is thought to be a solution to the shortage in organ donors. Many considerations favor pigs as the most suitable donor species for clinical xenotransplantation. The pig-to-nonhuman primate experiments are a prelude to clinical xenotransplantation, and prolonged survival has been achieved in this model. However, for kidney transplantation, vascular complications and EPO incompatibility represent major problems. Therefore, we established a novel stable pig-tomonkey kidney transplantation model.
METHODS Animals All donors were 3– 4-week-old Hubei White pigs, weighing 3.5 to 4.5 kg. The recipients were 3–5-year-old monkeys from Kunming China (Macaca mulata) 3 to 5 kg weight.
tion (HAR), 4/10 survived for up to 4 days, 1/10 died of gastric hemorrhage, and 1/10 died of massive intra-abdominal bleeding. DISCUSSION
The relative absence of HAR inevitably leads to the doubt about whether the cynomolgus monkey is a suitable recipient in a preclinical model. In our opinion, since HAR can be prevented, the occurence of HAR in the model is not important. The pig-to-monkey kidney xenotransplantation model can be used in research if surgical complications can be avoided. Although there is some prior experience in pig-to-nonhuman primate kidney xenotransplantation,1,2 we found that end-to-side anastomosis between abdominal aorta of donor and recipient improved outcomes using the right native kidney bed. REFERENCES
Surgical Procedure According to the different manner of vascular anastomosis, recipients were divided into two groups. In group I the abdominal aorta of donor with the right renal artery attached, was anastomosed end-to-side to the abdominal aorta of the recipient. In group II, an end-to-side anastomosis was constructed between the renal artery of the donor and the abdominal aorta of the recipient. The right native kidney was removed, and the left kidney remained. The left ureter of the recipient was ligated. It was regarded as a successful case if the graft recovered with good urine output.
RESULTS
Among group I hosts 12/14 recipients experienced a vascular complication. Among the remaining animals, 1/14 underwent hyperacute rejection; 1/14 survived for 8 days. Among group II, 4/10 subjects underwent hyperacute rejec-
© 2001 by Elsevier Science Inc. 655 Avenue of the Americas, New York, NY 10010 Transplantation Proceedings, 33, 3859 (2001)
1. Qi S, Peng J, Xu D, et al: Microsurgery 19:335, 1999 2. Cozzi E, Bhatti F, Schmoeckel M, et al: Transplantation 70:15, 2000 From the Organ Transplantation Institute, Tongji Medical College of Huazhong University of Science and Technology (G.C., S.C., S.Q.S., T.Z., H.W., Y.W., L.J.C., J.G.Z.) and Department of Surgery, Tianjin Second Central Hospital (X.M.W.), Wuhan, China. This work was supported by a grant from the National Natural Science Foundation of China and by the National High-Technology Research and Development Program (863 Program) of China. Address reprint requests to Shi Chen, PhD, Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang St, Wuhan 430030, China.
0041-1345/01/$–see front matter PII S0041-1345(01)02635-5 3859
I
N the last few years, important progress in xenotransplantation has been stimulated by a shortage of organs available for clinical use. Xenotransplantation is thought to be a solution to the shortage in organ donors. Many considerations favor pigs as the most suitable donor species for clinical xenotransplantation. The pig-to-nonhuman primate experiments are a prelude to clinical xenotransplantation, and prolonged survival has been achieved in this model. However, for kidney transplantation, vascular complications and EPO incompatibility represent major problems. Therefore, we established a novel stable pig-tomonkey kidney transplantation model.
METHODS Animals All donors were 3– 4-week-old Hubei White pigs, weighing 3.5 to 4.5 kg. The recipients were 3–5-year-old monkeys from Kunming China (Macaca mulata) 3 to 5 kg weight.
tion (HAR), 4/10 survived for up to 4 days, 1/10 died of gastric hemorrhage, and 1/10 died of massive intra-abdominal bleeding. DISCUSSION
The relative absence of HAR inevitably leads to the doubt about whether the cynomolgus monkey is a suitable recipient in a preclinical model. In our opinion, since HAR can be prevented, the occurence of HAR in the model is not important. The pig-to-monkey kidney xenotransplantation model can be used in research if surgical complications can be avoided. Although there is some prior experience in pig-to-nonhuman primate kidney xenotransplantation,1,2 we found that end-to-side anastomosis between abdominal aorta of donor and recipient improved outcomes using the right native kidney bed. REFERENCES
Surgical Procedure According to the different manner of vascular anastomosis, recipients were divided into two groups. In group I the abdominal aorta of donor with the right renal artery attached, was anastomosed end-to-side to the abdominal aorta of the recipient. In group II, an end-to-side anastomosis was constructed between the renal artery of the donor and the abdominal aorta of the recipient. The right native kidney was removed, and the left kidney remained. The left ureter of the recipient was ligated. It was regarded as a successful case if the graft recovered with good urine output.
RESULTS
Among group I hosts 12/14 recipients experienced a vascular complication. Among the remaining animals, 1/14 underwent hyperacute rejection; 1/14 survived for 8 days. Among group II, 4/10 subjects underwent hyperacute rejec-
© 2001 by Elsevier Science Inc. 655 Avenue of the Americas, New York, NY 10010 Transplantation Proceedings, 33, 3859 (2001)
1. Qi S, Peng J, Xu D, et al: Microsurgery 19:335, 1999 2. Cozzi E, Bhatti F, Schmoeckel M, et al: Transplantation 70:15, 2000 From the Organ Transplantation Institute, Tongji Medical College of Huazhong University of Science and Technology (G.C., S.C., S.Q.S., T.Z., H.W., Y.W., L.J.C., J.G.Z.) and Department of Surgery, Tianjin Second Central Hospital (X.M.W.), Wuhan, China. This work was supported by a grant from the National Natural Science Foundation of China and by the National High-Technology Research and Development Program (863 Program) of China. Address reprint requests to Shi Chen, PhD, Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang St, Wuhan 430030, China.
0041-1345/01/$–see front matter PII S0041-1345(01)02635-5 3859