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Addendum to Volume 90, Issue 2
Journal of Reproductive Immunology 93 (2012) 123
Contents lists available at SciVerse ScienceDirect
Journal of Reproductive Immunology journal homepage: www.elsevier.com/locate/jreprimm
Addendum
Addendum to Volume 90, Issue 2
The following abstract was presented as a poster presentation at the 9th Congress of the European Society for Reproductive Immunology and European Society for Human Reproduction and Embryology Early Pregnancy Special interest Group, August 23–26, Copenhagen, Denmark. P39 Gene panel in human cumulus cells as a biomarker for successful in vitro procedures K. Todorova, S. Hayrabedyan, J. Dineva, I. Vangelov, M. Ivanova, M. Mollova Institute of Biology and Immunology of Reproduction “Akad. K. Bratanov”, Bulgarian Academy of Sciences, 73 Tsarigardsko shosse, 1113 Sofia, Bulgaria
Introduction: Selecting oocytes with normal developmental competence would affect further embryo implantation potential and is of great importance in assisted reproductive technology. Based on the importance of cumulus–oocyte interplay, we suspect that the expression of specific genes in cumulus cells would affect the developmental competence of the oocyte and these genes might be used as prognostic indicators for a successful in vitro procedure. Methods: We investigated cumulus cell samples (n = 39) from women with reproductive problems who
DOI of the original article:10.1016/j.jri.2011.06.059. 0165-0378/$ – see front matter doi:10.1016/j.jri.2012.02.001
were participating in ICSI procedures, as well as cumulus cell samples (n = 9) from women from male-factor sterility couples. The total number of women was 16. In our study we estimated the differential gene expression levels of selected genes in the cumulus cells surrounding the acquired oocytes from ICSI-targeted primary and secondary sterility IVF patients and healthy women. The mRNA transcripts of enzyme and scavenger receptor lysil oxidase (LOX) and the Ig-superfamily member basigin (BSG), which is known to be important in embryo implantation, were assessed using reverse-transcriptase quantitative real-time PCR and data were normalized to phosphoglycerate kinase (PGK1). Results: We investigated for the first time in human the expression levels of lysil oxidase in cumulus cells. We observed a 4.32-fold increase in median LOX mRNA transcript levels and a 6.50-fold increase in median BSG mRNA transcript levels in women with primary sterility compared to healthy women. The difference observed in women with secondary sterility was also increased compared to the healthy women—3.85-fold for median LOX mRNA and 2.50fold for median BSG mRNA transcript levels. The coefficient of determination (r2 ) between LOX and BSG mRNAs was 0.5. Conclusions: Our study suggests that LOX and BSG genes need further evaluation as putative candidate markers for non-invasive assessment of oocyte competence. Keywords: Oocytes competence; Human cumulus cells; LOX; BSG
Contents lists available at SciVerse ScienceDirect
Journal of Reproductive Immunology journal homepage: www.elsevier.com/locate/jreprimm
Addendum
Addendum to Volume 90, Issue 2
The following abstract was presented as a poster presentation at the 9th Congress of the European Society for Reproductive Immunology and European Society for Human Reproduction and Embryology Early Pregnancy Special interest Group, August 23–26, Copenhagen, Denmark. P39 Gene panel in human cumulus cells as a biomarker for successful in vitro procedures K. Todorova, S. Hayrabedyan, J. Dineva, I. Vangelov, M. Ivanova, M. Mollova Institute of Biology and Immunology of Reproduction “Akad. K. Bratanov”, Bulgarian Academy of Sciences, 73 Tsarigardsko shosse, 1113 Sofia, Bulgaria
Introduction: Selecting oocytes with normal developmental competence would affect further embryo implantation potential and is of great importance in assisted reproductive technology. Based on the importance of cumulus–oocyte interplay, we suspect that the expression of specific genes in cumulus cells would affect the developmental competence of the oocyte and these genes might be used as prognostic indicators for a successful in vitro procedure. Methods: We investigated cumulus cell samples (n = 39) from women with reproductive problems who
DOI of the original article:10.1016/j.jri.2011.06.059. 0165-0378/$ – see front matter doi:10.1016/j.jri.2012.02.001
were participating in ICSI procedures, as well as cumulus cell samples (n = 9) from women from male-factor sterility couples. The total number of women was 16. In our study we estimated the differential gene expression levels of selected genes in the cumulus cells surrounding the acquired oocytes from ICSI-targeted primary and secondary sterility IVF patients and healthy women. The mRNA transcripts of enzyme and scavenger receptor lysil oxidase (LOX) and the Ig-superfamily member basigin (BSG), which is known to be important in embryo implantation, were assessed using reverse-transcriptase quantitative real-time PCR and data were normalized to phosphoglycerate kinase (PGK1). Results: We investigated for the first time in human the expression levels of lysil oxidase in cumulus cells. We observed a 4.32-fold increase in median LOX mRNA transcript levels and a 6.50-fold increase in median BSG mRNA transcript levels in women with primary sterility compared to healthy women. The difference observed in women with secondary sterility was also increased compared to the healthy women—3.85-fold for median LOX mRNA and 2.50fold for median BSG mRNA transcript levels. The coefficient of determination (r2 ) between LOX and BSG mRNAs was 0.5. Conclusions: Our study suggests that LOX and BSG genes need further evaluation as putative candidate markers for non-invasive assessment of oocyte competence. Keywords: Oocytes competence; Human cumulus cells; LOX; BSG