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Albumin administration in clinical practice, Ghaem Teaching Hospital, Mashhad
S232
Abstracts
maternal age showed a relation (r = 0.194, P = 0.023) and with gestational age showed an inverse relation(r = −0.263, P = 0.002). There were no other differences between TAC with other variables. Conclusions: Findings showed that the reduction of plasma TAC in sixth month pregnancy is as a prediction factor stress oxidative in pregnant women. Keywords: Total antioxidant capacity, Pregnant, Women doi:10.1016/j.clinbiochem.2011.08.561
Poster – [A-10-787-1] Albumin administration in clinical practice, Ghaem Teaching Hospital, Mashhad Siadat Zahraa, Norouzy Abdolrezab, Nematy Mohsenb, Safarian Mohammadb a Masshad University of Medical Sciences, Mashhad, Iran b Mashhad, Iran E-mail addresses: [email protected] (S. Zahra), [email protected] (N. Abdolreza), [email protected] (N. Mohsen), [email protected] (S. Mohammad) Introduction: Albumin is a protein derived from blood which accounts for 60%–80% of normal plasma oncotic pressure. Albumin administration is an expensive therapy, the use of which is subject to controversy and uncertainty. We designed this study to evaluate the administration of albumin in Ghaem hospital. Material and method: All of the 18–70 years old patients in the hospital who received albumin were identified. Thirteen out of the 18 albumin receivers were hospitalized in ICU wards. In 5 cases, serum albumin was not measured before its administration. Result: According to lab results, only 2 of the patients had albumin less than 2.5 g/dl. Pitting edema in ankle was observed in 14 patients and only one patient had CRP test results. Malnutrition was assessed according to criteria in MUST form. Conclusion: Five patients were slightly, 11 moderately and 2 severely malnourished. Our results indicate that albumin administration in Ghaem hospital was not according to suggested protocols in all cases. One of the major problems in Ghaem hospital is inappropriate prescription of albumin which can exert serious consequences on patients' health outcome. We suggest clinical practice guidelines should be established and used for albumin administration in hospitals. Keywords: Albumin administration, Ghaem hospital doi:10.1016/j.clinbiochem.2011.08.562
Poster – [A-10-792-1] Effects of tartrazine colorant on DNA structure Kashanian Soheila Razi University, Iran E-mail address: [email protected] Tartrazine is one of the most widely used artificial foods, drugs and cosmetic dyes. It is a nitrous derivative and is known to cause allergic reactions such as asthma and urticaria. To elucidate a possible tartrazine binding to DNA base pairs an experiment was performed by monitoring the spectroscopic changes in the UV–Vis. Hyperchromism in UV absorption bands of tartrazine and DNA was observed. Amino and hydroxyl groups of tartrazine could form hydrogen bonds
with the DNA base pairs, contributing to the overall hyperchromism and damage of the DNA double-helix structure. These results prove that tartrazine interacts with CT-DNA via groove binding mode with the intrinsic binding constant of 3.75 × 104. We conclude that, more care must be done to prevent our children from eating or drinking large amounts of food containing this colorant. Keywords: DNA, Tartrazine, Groove binding doi:10.1016/j.clinbiochem.2011.08.563
Poster – [A-10-827-1] DNA binding studies of tartrazine food additive Kashanian Soheila, Heidary Zeidali Sahar Razi University, Kermanshah, Iran E-mail addresses: [email protected] (K. Soheila), [email protected] (H.Z. Sahar) The interaction of native calf thymus DNA with tartrazine in 10 mM Tris–HCl aqueous solution at neutral pH 7.4 has been investigated. Tartrazine is a nitrous derivative which induces oxidative stress and DNA damage. Its DNA-binding properties were studied by means of CD spectra, competitive binding with Hoechst 33258 and viscosity measurements. It is found that tartrazine molecules can bind to DNA via groove mode nature as evidenced by: decrease in Hoechst–DNA solution fluorescence, unchanged viscosity of DNA and certain conformational changes, such as the conversion from a more B-like to a more C-like in the circular dichroism spectra of DNA. Our results indicate that this food colorant has a toxic potential to CT-DNA in vitro and it seems that it binds directly to DNA. We conclude that, more care must be done to prevent our children from eating or drinking large amounts of food containing this colorant. Keywords: DNA, Tartrazine, Groove binding mode doi:10.1016/j.clinbiochem.2011.08.564
Poster – [A-10-827-2] Circular dichroism (CD) and viscometric studies on tartrazine bound to calf thymus DNA Kashanian Soheila, Heidary Zeidali Sahar Razi University, Kermanshah, Iran E-mail addresses: [email protected] (K. Soheila), [email protected] (H.Z. Sahar) The interaction of native calf thymus DNA with tartrazine in 10 mM Tris–HCl aqueous solution at neutral pH 7.4 has been investigated. Tartrazine is a nitrous derivative which induces oxidative stress and DNA damage. Its DNA-binding properties were studied by means of CD spectra, competitive binding with Hoechst 33258 and viscosity measurements. It is found that tartrazine molecules can bind to DNA via groove mode nature as evidenced by decreasing in Hoechst–DNA solution fluorescence, unchanged viscosity of DNA and certain conformational changes, such as the conversion from a more B-like to a more C-like in the circular dichroism spectra of DNA. Our results indicate that this food colorant has a toxic potential to CT-DNA in vitro and it seems that it binds directly to DNA. We conclude that, more attention must be done to prevent our children from eating or drinking large amounts of food containing this colorant. Keywords: Groove binding, Circular dichroism doi:10.1016/j.clinbiochem.2011.08.565
Abstracts
maternal age showed a relation (r = 0.194, P = 0.023) and with gestational age showed an inverse relation(r = −0.263, P = 0.002). There were no other differences between TAC with other variables. Conclusions: Findings showed that the reduction of plasma TAC in sixth month pregnancy is as a prediction factor stress oxidative in pregnant women. Keywords: Total antioxidant capacity, Pregnant, Women doi:10.1016/j.clinbiochem.2011.08.561
Poster – [A-10-787-1] Albumin administration in clinical practice, Ghaem Teaching Hospital, Mashhad Siadat Zahraa, Norouzy Abdolrezab, Nematy Mohsenb, Safarian Mohammadb a Masshad University of Medical Sciences, Mashhad, Iran b Mashhad, Iran E-mail addresses: [email protected] (S. Zahra), [email protected] (N. Abdolreza), [email protected] (N. Mohsen), [email protected] (S. Mohammad) Introduction: Albumin is a protein derived from blood which accounts for 60%–80% of normal plasma oncotic pressure. Albumin administration is an expensive therapy, the use of which is subject to controversy and uncertainty. We designed this study to evaluate the administration of albumin in Ghaem hospital. Material and method: All of the 18–70 years old patients in the hospital who received albumin were identified. Thirteen out of the 18 albumin receivers were hospitalized in ICU wards. In 5 cases, serum albumin was not measured before its administration. Result: According to lab results, only 2 of the patients had albumin less than 2.5 g/dl. Pitting edema in ankle was observed in 14 patients and only one patient had CRP test results. Malnutrition was assessed according to criteria in MUST form. Conclusion: Five patients were slightly, 11 moderately and 2 severely malnourished. Our results indicate that albumin administration in Ghaem hospital was not according to suggested protocols in all cases. One of the major problems in Ghaem hospital is inappropriate prescription of albumin which can exert serious consequences on patients' health outcome. We suggest clinical practice guidelines should be established and used for albumin administration in hospitals. Keywords: Albumin administration, Ghaem hospital doi:10.1016/j.clinbiochem.2011.08.562
Poster – [A-10-792-1] Effects of tartrazine colorant on DNA structure Kashanian Soheila Razi University, Iran E-mail address: [email protected] Tartrazine is one of the most widely used artificial foods, drugs and cosmetic dyes. It is a nitrous derivative and is known to cause allergic reactions such as asthma and urticaria. To elucidate a possible tartrazine binding to DNA base pairs an experiment was performed by monitoring the spectroscopic changes in the UV–Vis. Hyperchromism in UV absorption bands of tartrazine and DNA was observed. Amino and hydroxyl groups of tartrazine could form hydrogen bonds
with the DNA base pairs, contributing to the overall hyperchromism and damage of the DNA double-helix structure. These results prove that tartrazine interacts with CT-DNA via groove binding mode with the intrinsic binding constant of 3.75 × 104. We conclude that, more care must be done to prevent our children from eating or drinking large amounts of food containing this colorant. Keywords: DNA, Tartrazine, Groove binding doi:10.1016/j.clinbiochem.2011.08.563
Poster – [A-10-827-1] DNA binding studies of tartrazine food additive Kashanian Soheila, Heidary Zeidali Sahar Razi University, Kermanshah, Iran E-mail addresses: [email protected] (K. Soheila), [email protected] (H.Z. Sahar) The interaction of native calf thymus DNA with tartrazine in 10 mM Tris–HCl aqueous solution at neutral pH 7.4 has been investigated. Tartrazine is a nitrous derivative which induces oxidative stress and DNA damage. Its DNA-binding properties were studied by means of CD spectra, competitive binding with Hoechst 33258 and viscosity measurements. It is found that tartrazine molecules can bind to DNA via groove mode nature as evidenced by: decrease in Hoechst–DNA solution fluorescence, unchanged viscosity of DNA and certain conformational changes, such as the conversion from a more B-like to a more C-like in the circular dichroism spectra of DNA. Our results indicate that this food colorant has a toxic potential to CT-DNA in vitro and it seems that it binds directly to DNA. We conclude that, more care must be done to prevent our children from eating or drinking large amounts of food containing this colorant. Keywords: DNA, Tartrazine, Groove binding mode doi:10.1016/j.clinbiochem.2011.08.564
Poster – [A-10-827-2] Circular dichroism (CD) and viscometric studies on tartrazine bound to calf thymus DNA Kashanian Soheila, Heidary Zeidali Sahar Razi University, Kermanshah, Iran E-mail addresses: [email protected] (K. Soheila), [email protected] (H.Z. Sahar) The interaction of native calf thymus DNA with tartrazine in 10 mM Tris–HCl aqueous solution at neutral pH 7.4 has been investigated. Tartrazine is a nitrous derivative which induces oxidative stress and DNA damage. Its DNA-binding properties were studied by means of CD spectra, competitive binding with Hoechst 33258 and viscosity measurements. It is found that tartrazine molecules can bind to DNA via groove mode nature as evidenced by decreasing in Hoechst–DNA solution fluorescence, unchanged viscosity of DNA and certain conformational changes, such as the conversion from a more B-like to a more C-like in the circular dichroism spectra of DNA. Our results indicate that this food colorant has a toxic potential to CT-DNA in vitro and it seems that it binds directly to DNA. We conclude that, more attention must be done to prevent our children from eating or drinking large amounts of food containing this colorant. Keywords: Groove binding, Circular dichroism doi:10.1016/j.clinbiochem.2011.08.565