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Ameloblastic fibrodentinoma: A rare case in maxilla
OOOO Volume 114, Number 4 grading (p⫽0.000). The immunoreactivity of CA-IX exhibited correlation with histological grading (p⫽0.005). Conclusion: it can be concluded that there is a broad participation of these markers of hypoxia in oral carcinogenesis.
INTERCALATED DUCT LESIONS OF SALIVARY GLAND: CLINICOPATHOLOGICAL CHARACTERIZATION Montalli VAM1, Costa AF, Martinez EF2, Altemani A1 - 1UNIVERSIDADE ESTADUAL DE CAMPINAS - FACULDADE DE CIÊNCIAS MÉDICAS - ANATOMIA PATOLÓGICA, 2 INTITUTO E CENTRO DE PESQUISAS SÃO LEOPOLDO MANDIC - PATOLOGIA Background/objective: We aimed to characterize the clinicopathologic features of intercalated duct lesions (IDLs) of salivary glands. Study design: Seven patients with IDLs are presented. Nonencapsulated ductal proliferations were classified as hyperplastic IDL (HIDL) and those completely encapsulated as adenoma. Results: The patients ranged in age from 19 to 88 years. All IDLs were parotid lesions ranging from 1 to 9mm. In six cases (3 HIDLs and 3 adenomas), the IDLs were unifocal lesions. In only one case, the lesion (adenoma) was symptomatic leading to surgical resection. In the remaining 6 cases, the parotid gland had been removed for other reasons and basal cell adenoma (BCA) was the main disease in two of them. Conclusion: Owing possibly to its small size, IDLs are usually asymptomatic lesions. The association between IDLs and BCAS reinforces the hypothesis that IDLs could be precursor lesions of BCA. FAPESP grants: 2011/10366-7 and 11/51112-8.
DYSPLASIA AND MICROINVASIVE CARCINOMA IN A BRAZILIAN ORAL MEDICINE SERVICE Valente VB1,2,3,4,5 - 1ARARAQUARA SCHOOL OF DENTISTRY - UNESP DEPARTMENT OF DIAGNOSIS AND SURGERY, 2ELAINE MARIA SGAVIOLI MASSUCATO - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY, 3MIRIAN APARECIDA ONOFRE - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY, 4CLEVERTON ROBERTO DE ANDRADE - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY, 5CLÁUDIA MARIA NAVARRO - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY Objective: Clinicopathological evaluation of patients with dysplasia (D) and microinvasive oral carcinoma (MC). Study design: Retrospective study with files of patients with D and MC attended at the Oral Medicine Service, Araraquara School of Dentistry (2009 to 2011). Results: Of 19 cases, 4 were MCs and 15 Ds, being the patients predominantly men (13), white (19) with an average age of 55 years. The main anatomical sites were: lips (7), alveolar ridge (5) and buccal mucosa (4). It was observed exposure to tobacco (9), alcohol (6) and solar radiation (7). Six patients had a familiar history for cancer (breast, prostate, skin and mouth). The main systemic diseases were hypertension (4), depression (3) and diabetes (2). Leukoplakias (8), Actinic Cheilitis (4) and Lichen Planus (3) were the most frequent clinical diagnosis. Conclusion: It is emphasized, thus, the importance of early diagnoses of cancer avoiding mutilating treatments and poor quality of life.
ORAL ABSTRACTS Abstracts e73 KOLSCHÜTTER SYNDROME – REPORT OF A NEW CASE González-Arriagada WA1, Carlos-Bregni R2, Contreras E2, Vargas PA1, Lopes MA1 - 1FOP-UNICAMP - ESTOMATOPATOLOGIA, 2CENTRO CLÍNICO DE CABEZA Y CUELLO - CIUDAD DE GUATEMALA, GUATEMALA Introduction: Kolschütter Syndrome is a rare disorder clinically characterized by amelogenesis imperfecta, epilepsy and progressive mental deterioration. The aim of this article is to describe an additional clinical case of this syndrome. Case report: A 9-year-old boy was referred for evaluation of teeth pigmentation. With 8 years of age he presented generalized tonic-clonic seizure, treated with valproic acid. The boy also manifested mental deterioration and hyperactivity. On physical examination he showed a symmetrical face with reduction in size of the lower third, slightly palpebral fissures, low ear and hair implantation, coarse hair and hypertrichosis. The intraoral examination displayed crowding teeth with generalized enamel defects, suggesting the clinical diagnosis of amelogenesis imperfecta with a yellow-brownish coloration, a high incidence of caries. Conclusions: It is important that dentists be able to recognize this syndrome for better management of the patients.
AUTOFLUORESCENCE PATTERNS OF ORAL LESIONS: PRELIMINARY RESULTS Barcelos JS1, Azevedo RS1, Pereira LB1, Kurachi C2, Fontes KBFC1, Camisasca DR1, Takahama Júnior A1 - 1UNIVERSIDADE FEDERAL FLUMINENSE - FACULDADE DE ODONTOLOGIA - PÓLO UNIVERSITÁRIO DE NOVA FRIBURGO, 2UNIVERSIDADE DE SÃO PAULO - INSTITUTO DE FÍSICA DE SÃO CARLOS Background: The autofluorescence is a non-invasive diagnostic tool that has been used to facilitate the detection of premalignant or malignant changes of the oral mucosa. Different patterns of fluorescence can be related to differences in biochemical composition of the tissue and malignant changes usually show loss of fluorescence. Objectives: The objective of this study is to determinate the patterns of autofluorescence of various types of oral lesions. Study design: For this, all lesions indicated for surgical biopsy are visualized and photographed using a wild field device which emits blue light of 400 to 460 nm to excite green fluorescence in the oral tissues. The images were classified according to the retention or emission of green fluorescence. Results: Preliminary results indicate that most of lesions showed loss of fluorescence compared to normal tissue. Conclusion: We can conclude that the loss of fluorescence is not specific for malignant changes.
AMELOBLASTIC FIBRODENTINOMA: A RARE CASE IN MAXILLA Gomes TG1, Silva RV2, Medeiros N2, Spyrides KS2, Resende RFB2, Oliveira SP3, Alves ATNN2 - 1UNIVERSIDADE GAMA FILHO - ODONTOLOGIA GRADUAÇÃO, 2 UNIVERSIDADE GAMA FILHO - ODONTOLOGIA, 3 OCEX - ESTOMATOLOGIA Ameloblastic fibrodentinoma (AFD) is a rare benign odontogenic tumor, is composed of proliferating odontogenic epithelium in a cellular ectomesenchyme resembling dental papilla with dentin or dentin-like formation. The present case report a 9-yearold male reported in dental school of Universidade Gama Filho.
OOOO
e74
Abstracts
On extraoral examination we notice a painless swelling in right maxilla. Intraoral examination, an expansive swelling in buccal vestibule was notice and teeth 26 were missing. The Orthopantomograph revealed a radiolucency ill-defined and displaced of unerupted permanents teeth. CT revealed a expansile lesion in maxilla with diminute calcifications. Incisional biopsy was performed and submit for histopathological analysis. The epithelial component of the tumour consists of anastomosing epithelial strands and nest, resembles ameloblastoma. The stromal component is an immature cell-rich myxoid tissue. Juxtaepithelial hyalinization was evident and amount of dentin or dentin-like matrix also was seen. The proposed treatment consisted of enucleation and curettage.
A HIGH HIF-1〈 EXPRESSION GENOTYPES INCREASE RISK OF ORAL CANCER Sousa AA1, Fraga CAC2, Alves LR1, Bandeira GA3, Paula AMB1, Guimarães ALS - 1UNIMONTES - DEPARTMENT OF DENTISTRY, 2UNIMONTES - DEPARTMENT OF BIOLOGY, 3UNIMONTES - DEPARTMENT OF MEDICINE Background: A single nucleotide polymorphism of C1772T and G1790A of HIF-1␣ was reported to modulate susceptibility to cancer. Objective: We investigated the role of these polymorphic variants in relation to the risk of oral cancer. Study design: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the HIF-1␣ polymorphisms in 42 patients with oral squamous cell carcinoma (OSCC), 48 with oral leukoplakia (OL), as well as in 88 healthy control subjects. Results: TT and AA genotypes increased the risk of cancer. CT and GA genotypes were associated with OL risk, but not with epithelial dysplasia grading. We did not observe any difference in allelic frequencies between HNSCC and tumor size, lymph node metastasis and survival. Conclusion: In conclusion, the presence of T and A alleles might indicate a higher risk of leukoplakia progressing to cancer.
CENTRAL GIANT CELL GRANULOMA: REPORT OF TWO CLINICAL CASES Delgado AM1, Brandão AAH1, Alves MGO1, Marsi G1, Almeida JD1, Anbinder AL1, Cavalcante ASR1 - 1UNESP - DEPARTAMENTO DE DIAGNÓSTICO E BIOCIÊNCIAS Central giant cell granuloma (CGCG) is a benign proliferation of fibroblasts and multinucleated giant cells. It is usually seen in young adults. Mandible, anterior to the first molar, where the teeth have had deciduous predecessors, is the usual site. Clinical, histopathological and imaging features of two well documented cases are presented. Case 1- A Caucasian boy, 10 years-old, came with his mother complaining of swelling on the right side of mandible, asymptomatic, with about three years of evolution. Panoramic radiograph showed multilocular radiolucent area. Case 2 – A young Caucasian woman, 24 years-old, attended the service, reporting that after the extraction of teeth 35 and 37, she noticed a swelling in the region, with a pain and bleeding exophytic growth. Radiographic and tomographic images of a radiolucent region, deepened toward to the mandible base. Histopathological diagnosis was CGCG. Exams excluded hyperparathyroidism in both cases. Surgery was the chosen treatment.
October 2012 HUMAN PAPILLOMAVIRUS (HPV) FREQUENCY IN ORAL SQUAMOUS CELL CARCINOMA (OSCC) IN AMAZON POPULATION Jesus AS1, Abreu MC1, Silva BTC1, Pontes HAR1, Coracin FL2, Mello WA3, Silvestre RVD3 1 FEDERAL UNIVERSITY OF PARÁ - JOÃO DE BARROS BARRETO UNIVERSITY HOSPITAL, 2UNIVERSITY OF SÃO PAULO - DEPARTMENT OF STOMATOLOGY (ORAL PATHOLOGY), 3EVANDRO CHAGAS INSTITUTE - PAPILLOMAVIRUS LABORATORY Background/objective: The oral squamous cell carcinoma (OSCC) is the leading malignancy in the head and neck, with smoking as the major etiologic risk factor. The role of Human papillomavirus (HPV) in the etiology of this lesion is controversial, with some studies finding an important association. The purpose of this study was to investigate HPV DNA types in this disease. Study design: The presence of HPV was investigated by polymerase chain reaction in 74 specimens of OSCC paraffinembedded from a group of individuals diagnosed in amazon population. Results: From 74 tissue samples of OSCC, only one (1.35%) was HPV 16 positive. The sample was from a lesion in the oropharynx of a young female patient (⬍45 years) with no smoking habit. Conclusions: The results obtained in our specific population suggest that the HPV takes no part in the carcinogenesis of the OSCC. Future investigations will be needed to confirm our results.
ORAL SQUAMOUS CELL CARCINOMA PROLIFERATION AND INVASION UNDER LOW-LEVEL LASER IRRADIATION: IN VITRO STUDY Henriques ACG1, Pereira JS1, Ginani F1, Oliveira RM2, Barboza CAG1, Rocha HAO2, Freitas RA1 - 1UFRN - ODONTOLOGIA, 2UFRN - BIOQUÍMICA Objectives: to evaluate the effect of low-level laser (LLL) on oral squamous cell carcinoma cell line (SCC25) proliferation and invasion. Study design: Cultured cells were irradiated with an InGaAIP diode laser, 660nm, 30mW using two energy densities (0.5J/cm2 and 1.0J/cm2). Cell proliferation was assessed through Trypan Blue staining method and cyclin D1 expression by immunofluorescence at 0, 24, 48, and 72 hours after irradiation. An invasion assay was performed for 72 hours and the invasive cells were counted under light microscopy. Results: Proliferation curve demonstrated that SCC25 irradiated with 1.0J/cm2 had the highest proliferative rate when compared to the control group (P⬍.05) and the group irradiated with 0.5J/cm2 (P⬍.05) at the studied intervals. In addition, this same group expressed higher levels of cyclin D1 and significantly higher potential for invasion (P⬍.001). Conclusion: Our results indicate that LLL has an important stimulatory effect on proliferation and invasion of oral SCC cells.
MULTIPLE MYELOMA WITH EXTENSIVE MANDIBLE AND MAXILLARY INVOLVEMENT - A CASE REPORT Miranda AMMA, Pires FR, Almeida OP, Araújo AC, Baptista RL, Lucena SB, Queiroz C Multiple myeloma is a malignant neoplasic proliferation of immunoglobulin-producing plasma cells, which can rarely pres-
INTERCALATED DUCT LESIONS OF SALIVARY GLAND: CLINICOPATHOLOGICAL CHARACTERIZATION Montalli VAM1, Costa AF, Martinez EF2, Altemani A1 - 1UNIVERSIDADE ESTADUAL DE CAMPINAS - FACULDADE DE CIÊNCIAS MÉDICAS - ANATOMIA PATOLÓGICA, 2 INTITUTO E CENTRO DE PESQUISAS SÃO LEOPOLDO MANDIC - PATOLOGIA Background/objective: We aimed to characterize the clinicopathologic features of intercalated duct lesions (IDLs) of salivary glands. Study design: Seven patients with IDLs are presented. Nonencapsulated ductal proliferations were classified as hyperplastic IDL (HIDL) and those completely encapsulated as adenoma. Results: The patients ranged in age from 19 to 88 years. All IDLs were parotid lesions ranging from 1 to 9mm. In six cases (3 HIDLs and 3 adenomas), the IDLs were unifocal lesions. In only one case, the lesion (adenoma) was symptomatic leading to surgical resection. In the remaining 6 cases, the parotid gland had been removed for other reasons and basal cell adenoma (BCA) was the main disease in two of them. Conclusion: Owing possibly to its small size, IDLs are usually asymptomatic lesions. The association between IDLs and BCAS reinforces the hypothesis that IDLs could be precursor lesions of BCA. FAPESP grants: 2011/10366-7 and 11/51112-8.
DYSPLASIA AND MICROINVASIVE CARCINOMA IN A BRAZILIAN ORAL MEDICINE SERVICE Valente VB1,2,3,4,5 - 1ARARAQUARA SCHOOL OF DENTISTRY - UNESP DEPARTMENT OF DIAGNOSIS AND SURGERY, 2ELAINE MARIA SGAVIOLI MASSUCATO - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY, 3MIRIAN APARECIDA ONOFRE - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY, 4CLEVERTON ROBERTO DE ANDRADE - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY, 5CLÁUDIA MARIA NAVARRO - DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OF DENTISTRY Objective: Clinicopathological evaluation of patients with dysplasia (D) and microinvasive oral carcinoma (MC). Study design: Retrospective study with files of patients with D and MC attended at the Oral Medicine Service, Araraquara School of Dentistry (2009 to 2011). Results: Of 19 cases, 4 were MCs and 15 Ds, being the patients predominantly men (13), white (19) with an average age of 55 years. The main anatomical sites were: lips (7), alveolar ridge (5) and buccal mucosa (4). It was observed exposure to tobacco (9), alcohol (6) and solar radiation (7). Six patients had a familiar history for cancer (breast, prostate, skin and mouth). The main systemic diseases were hypertension (4), depression (3) and diabetes (2). Leukoplakias (8), Actinic Cheilitis (4) and Lichen Planus (3) were the most frequent clinical diagnosis. Conclusion: It is emphasized, thus, the importance of early diagnoses of cancer avoiding mutilating treatments and poor quality of life.
ORAL ABSTRACTS Abstracts e73 KOLSCHÜTTER SYNDROME – REPORT OF A NEW CASE González-Arriagada WA1, Carlos-Bregni R2, Contreras E2, Vargas PA1, Lopes MA1 - 1FOP-UNICAMP - ESTOMATOPATOLOGIA, 2CENTRO CLÍNICO DE CABEZA Y CUELLO - CIUDAD DE GUATEMALA, GUATEMALA Introduction: Kolschütter Syndrome is a rare disorder clinically characterized by amelogenesis imperfecta, epilepsy and progressive mental deterioration. The aim of this article is to describe an additional clinical case of this syndrome. Case report: A 9-year-old boy was referred for evaluation of teeth pigmentation. With 8 years of age he presented generalized tonic-clonic seizure, treated with valproic acid. The boy also manifested mental deterioration and hyperactivity. On physical examination he showed a symmetrical face with reduction in size of the lower third, slightly palpebral fissures, low ear and hair implantation, coarse hair and hypertrichosis. The intraoral examination displayed crowding teeth with generalized enamel defects, suggesting the clinical diagnosis of amelogenesis imperfecta with a yellow-brownish coloration, a high incidence of caries. Conclusions: It is important that dentists be able to recognize this syndrome for better management of the patients.
AUTOFLUORESCENCE PATTERNS OF ORAL LESIONS: PRELIMINARY RESULTS Barcelos JS1, Azevedo RS1, Pereira LB1, Kurachi C2, Fontes KBFC1, Camisasca DR1, Takahama Júnior A1 - 1UNIVERSIDADE FEDERAL FLUMINENSE - FACULDADE DE ODONTOLOGIA - PÓLO UNIVERSITÁRIO DE NOVA FRIBURGO, 2UNIVERSIDADE DE SÃO PAULO - INSTITUTO DE FÍSICA DE SÃO CARLOS Background: The autofluorescence is a non-invasive diagnostic tool that has been used to facilitate the detection of premalignant or malignant changes of the oral mucosa. Different patterns of fluorescence can be related to differences in biochemical composition of the tissue and malignant changes usually show loss of fluorescence. Objectives: The objective of this study is to determinate the patterns of autofluorescence of various types of oral lesions. Study design: For this, all lesions indicated for surgical biopsy are visualized and photographed using a wild field device which emits blue light of 400 to 460 nm to excite green fluorescence in the oral tissues. The images were classified according to the retention or emission of green fluorescence. Results: Preliminary results indicate that most of lesions showed loss of fluorescence compared to normal tissue. Conclusion: We can conclude that the loss of fluorescence is not specific for malignant changes.
AMELOBLASTIC FIBRODENTINOMA: A RARE CASE IN MAXILLA Gomes TG1, Silva RV2, Medeiros N2, Spyrides KS2, Resende RFB2, Oliveira SP3, Alves ATNN2 - 1UNIVERSIDADE GAMA FILHO - ODONTOLOGIA GRADUAÇÃO, 2 UNIVERSIDADE GAMA FILHO - ODONTOLOGIA, 3 OCEX - ESTOMATOLOGIA Ameloblastic fibrodentinoma (AFD) is a rare benign odontogenic tumor, is composed of proliferating odontogenic epithelium in a cellular ectomesenchyme resembling dental papilla with dentin or dentin-like formation. The present case report a 9-yearold male reported in dental school of Universidade Gama Filho.
OOOO
e74
Abstracts
On extraoral examination we notice a painless swelling in right maxilla. Intraoral examination, an expansive swelling in buccal vestibule was notice and teeth 26 were missing. The Orthopantomograph revealed a radiolucency ill-defined and displaced of unerupted permanents teeth. CT revealed a expansile lesion in maxilla with diminute calcifications. Incisional biopsy was performed and submit for histopathological analysis. The epithelial component of the tumour consists of anastomosing epithelial strands and nest, resembles ameloblastoma. The stromal component is an immature cell-rich myxoid tissue. Juxtaepithelial hyalinization was evident and amount of dentin or dentin-like matrix also was seen. The proposed treatment consisted of enucleation and curettage.
A HIGH HIF-1〈 EXPRESSION GENOTYPES INCREASE RISK OF ORAL CANCER Sousa AA1, Fraga CAC2, Alves LR1, Bandeira GA3, Paula AMB1, Guimarães ALS - 1UNIMONTES - DEPARTMENT OF DENTISTRY, 2UNIMONTES - DEPARTMENT OF BIOLOGY, 3UNIMONTES - DEPARTMENT OF MEDICINE Background: A single nucleotide polymorphism of C1772T and G1790A of HIF-1␣ was reported to modulate susceptibility to cancer. Objective: We investigated the role of these polymorphic variants in relation to the risk of oral cancer. Study design: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to determine the HIF-1␣ polymorphisms in 42 patients with oral squamous cell carcinoma (OSCC), 48 with oral leukoplakia (OL), as well as in 88 healthy control subjects. Results: TT and AA genotypes increased the risk of cancer. CT and GA genotypes were associated with OL risk, but not with epithelial dysplasia grading. We did not observe any difference in allelic frequencies between HNSCC and tumor size, lymph node metastasis and survival. Conclusion: In conclusion, the presence of T and A alleles might indicate a higher risk of leukoplakia progressing to cancer.
CENTRAL GIANT CELL GRANULOMA: REPORT OF TWO CLINICAL CASES Delgado AM1, Brandão AAH1, Alves MGO1, Marsi G1, Almeida JD1, Anbinder AL1, Cavalcante ASR1 - 1UNESP - DEPARTAMENTO DE DIAGNÓSTICO E BIOCIÊNCIAS Central giant cell granuloma (CGCG) is a benign proliferation of fibroblasts and multinucleated giant cells. It is usually seen in young adults. Mandible, anterior to the first molar, where the teeth have had deciduous predecessors, is the usual site. Clinical, histopathological and imaging features of two well documented cases are presented. Case 1- A Caucasian boy, 10 years-old, came with his mother complaining of swelling on the right side of mandible, asymptomatic, with about three years of evolution. Panoramic radiograph showed multilocular radiolucent area. Case 2 – A young Caucasian woman, 24 years-old, attended the service, reporting that after the extraction of teeth 35 and 37, she noticed a swelling in the region, with a pain and bleeding exophytic growth. Radiographic and tomographic images of a radiolucent region, deepened toward to the mandible base. Histopathological diagnosis was CGCG. Exams excluded hyperparathyroidism in both cases. Surgery was the chosen treatment.
October 2012 HUMAN PAPILLOMAVIRUS (HPV) FREQUENCY IN ORAL SQUAMOUS CELL CARCINOMA (OSCC) IN AMAZON POPULATION Jesus AS1, Abreu MC1, Silva BTC1, Pontes HAR1, Coracin FL2, Mello WA3, Silvestre RVD3 1 FEDERAL UNIVERSITY OF PARÁ - JOÃO DE BARROS BARRETO UNIVERSITY HOSPITAL, 2UNIVERSITY OF SÃO PAULO - DEPARTMENT OF STOMATOLOGY (ORAL PATHOLOGY), 3EVANDRO CHAGAS INSTITUTE - PAPILLOMAVIRUS LABORATORY Background/objective: The oral squamous cell carcinoma (OSCC) is the leading malignancy in the head and neck, with smoking as the major etiologic risk factor. The role of Human papillomavirus (HPV) in the etiology of this lesion is controversial, with some studies finding an important association. The purpose of this study was to investigate HPV DNA types in this disease. Study design: The presence of HPV was investigated by polymerase chain reaction in 74 specimens of OSCC paraffinembedded from a group of individuals diagnosed in amazon population. Results: From 74 tissue samples of OSCC, only one (1.35%) was HPV 16 positive. The sample was from a lesion in the oropharynx of a young female patient (⬍45 years) with no smoking habit. Conclusions: The results obtained in our specific population suggest that the HPV takes no part in the carcinogenesis of the OSCC. Future investigations will be needed to confirm our results.
ORAL SQUAMOUS CELL CARCINOMA PROLIFERATION AND INVASION UNDER LOW-LEVEL LASER IRRADIATION: IN VITRO STUDY Henriques ACG1, Pereira JS1, Ginani F1, Oliveira RM2, Barboza CAG1, Rocha HAO2, Freitas RA1 - 1UFRN - ODONTOLOGIA, 2UFRN - BIOQUÍMICA Objectives: to evaluate the effect of low-level laser (LLL) on oral squamous cell carcinoma cell line (SCC25) proliferation and invasion. Study design: Cultured cells were irradiated with an InGaAIP diode laser, 660nm, 30mW using two energy densities (0.5J/cm2 and 1.0J/cm2). Cell proliferation was assessed through Trypan Blue staining method and cyclin D1 expression by immunofluorescence at 0, 24, 48, and 72 hours after irradiation. An invasion assay was performed for 72 hours and the invasive cells were counted under light microscopy. Results: Proliferation curve demonstrated that SCC25 irradiated with 1.0J/cm2 had the highest proliferative rate when compared to the control group (P⬍.05) and the group irradiated with 0.5J/cm2 (P⬍.05) at the studied intervals. In addition, this same group expressed higher levels of cyclin D1 and significantly higher potential for invasion (P⬍.001). Conclusion: Our results indicate that LLL has an important stimulatory effect on proliferation and invasion of oral SCC cells.
MULTIPLE MYELOMA WITH EXTENSIVE MANDIBLE AND MAXILLARY INVOLVEMENT - A CASE REPORT Miranda AMMA, Pires FR, Almeida OP, Araújo AC, Baptista RL, Lucena SB, Queiroz C Multiple myeloma is a malignant neoplasic proliferation of immunoglobulin-producing plasma cells, which can rarely pres-