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Analysis of staphylococcal alpha toxin action in the taenia coli of the guinea pig
Abstracts for Card Indexes
543
Immunofluorescent detection ofenterotoxin B from S. aureus: U. Foxsum and A. FORSGREN, Toxicon, 1972, 10, 451 . (Institute of Medical Microbiology, University of Uppsala, Uppsala, Sweden) . Abstract-The well-known fact that many Staphylococcus aureus strains react with antisera to other bacteria and normal sera has been a major problem in immunofluorescent detection of various bacterial antigens. A reaction between protein A and the Fc-portion of IgG from different species is now well documented. Staphylococcal strains with a high content of protein A reacted strongly with FTTC-labelled IgG from nonimmunized rabbits and rabbits immunized against various bacterial antigens . Pepsin digestion of the IgG eliminated the nonspecific reaction thus showing that the Fc-part of the IgG is involved . The specific immunological activity of the F(abl),-fragments was intact . FITC-coAjugated IgG and F(abl), from rabbits immunized with purified enterotoxin B was used for detection of enterotoxin B with immunofluorescence . S. aureus strains with a high production of enterotoxin B and protein A, reacted in immunofluorescence with IgG but not F(ab'),. Enterotoxin B positive but protein A negative strains failed to react with 1gG or F(abl)6. In all experiments antigonococcal IgG were included before pepsin digestion and conjugation as a control. In this system the same titre was recorded with both IgG and F(ab'),. Thefindings suggest that enterotoxin B is not detectable on thesurfaceof S. aureus with immunofluorescence methods in current use.
Transport of drugs across the intestinal barrier of rats intoxicated with streptolysin O and staphylococcal alpha toxin : E. HADA§ovk, Toxicon, 1972, 10, 457. (Department of Pharmacology, School of Medicine, Brno, Czechoslovakia). Abstract--The influence of streptolysin O and staphylococcal alpha toxin on the in vitro transport of water and sulfamethoxydiazine from the lumen of rat intestine has been studied. Streptolysin O inhibited the transport of water and sulfamethoxydiazine, when the lysin was applied into the solution circulating in the lumen of intestine and when it was injected intravenously . The staphylococcal toxin had no effect on the transports of water or sulfamethoxydiazinewhen administered in vitro into thesolution in thelumen. In rats intoxicated by previous injection of staphylococcal toxin the transport ofwater and sulfamethoxydiazine wasinfluenced biphasically. In the fast phase the transports were slightly increased, after which inhibition of transportwas observed . The dependence of the in vitro water transport on the concentrations of glucose in the solution is described.
Analysis ofstaphylococcal alpha toxin action in the taenia coli ofthe guinea pig : O. KADLEc and 1. SEFERNA, Toxicon, 1972, 10, 465 . (Institute of Pharmacology, Czechoslovak Academy of Sciences and Department of Pharmacology, Faculty of Pediatrics, Àlbertov 4, Prague 2, Czechoslovakia) . AbstractThe effects of staphylococcal alpha toxin wero studied in taenia coli of the guinea pig by the sucrose gap method . The toxin evoked a dose-dependent depolarization, increase in action potential frequency and contraction during its contact time. Following removal of the toxin there was a block in responsiveness of the taenia toward 80 mM KCl or acetylcholine whereas the responsiveness toward histamine and BaC1, was only slightly reduced. Partial restoration of the excitability of the preparation was achieved by application of higher calcium concentrations . The effects of the toxin were similar to that of ouabain. On the depolarized preparation the toxin had no effect, the loosely bound fraction of membrane calcium being already released by depolarisation. It is concluded that the toxin may release the loosely bound fraction of membrane calcium. TOXICON1972 Vol. 10.
543
Immunofluorescent detection ofenterotoxin B from S. aureus: U. Foxsum and A. FORSGREN, Toxicon, 1972, 10, 451 . (Institute of Medical Microbiology, University of Uppsala, Uppsala, Sweden) . Abstract-The well-known fact that many Staphylococcus aureus strains react with antisera to other bacteria and normal sera has been a major problem in immunofluorescent detection of various bacterial antigens. A reaction between protein A and the Fc-portion of IgG from different species is now well documented. Staphylococcal strains with a high content of protein A reacted strongly with FTTC-labelled IgG from nonimmunized rabbits and rabbits immunized against various bacterial antigens . Pepsin digestion of the IgG eliminated the nonspecific reaction thus showing that the Fc-part of the IgG is involved . The specific immunological activity of the F(abl),-fragments was intact . FITC-coAjugated IgG and F(abl), from rabbits immunized with purified enterotoxin B was used for detection of enterotoxin B with immunofluorescence . S. aureus strains with a high production of enterotoxin B and protein A, reacted in immunofluorescence with IgG but not F(ab'),. Enterotoxin B positive but protein A negative strains failed to react with 1gG or F(abl)6. In all experiments antigonococcal IgG were included before pepsin digestion and conjugation as a control. In this system the same titre was recorded with both IgG and F(ab'),. Thefindings suggest that enterotoxin B is not detectable on thesurfaceof S. aureus with immunofluorescence methods in current use.
Transport of drugs across the intestinal barrier of rats intoxicated with streptolysin O and staphylococcal alpha toxin : E. HADA§ovk, Toxicon, 1972, 10, 457. (Department of Pharmacology, School of Medicine, Brno, Czechoslovakia). Abstract--The influence of streptolysin O and staphylococcal alpha toxin on the in vitro transport of water and sulfamethoxydiazine from the lumen of rat intestine has been studied. Streptolysin O inhibited the transport of water and sulfamethoxydiazine, when the lysin was applied into the solution circulating in the lumen of intestine and when it was injected intravenously . The staphylococcal toxin had no effect on the transports of water or sulfamethoxydiazinewhen administered in vitro into thesolution in thelumen. In rats intoxicated by previous injection of staphylococcal toxin the transport ofwater and sulfamethoxydiazine wasinfluenced biphasically. In the fast phase the transports were slightly increased, after which inhibition of transportwas observed . The dependence of the in vitro water transport on the concentrations of glucose in the solution is described.
Analysis ofstaphylococcal alpha toxin action in the taenia coli ofthe guinea pig : O. KADLEc and 1. SEFERNA, Toxicon, 1972, 10, 465 . (Institute of Pharmacology, Czechoslovak Academy of Sciences and Department of Pharmacology, Faculty of Pediatrics, Àlbertov 4, Prague 2, Czechoslovakia) . AbstractThe effects of staphylococcal alpha toxin wero studied in taenia coli of the guinea pig by the sucrose gap method . The toxin evoked a dose-dependent depolarization, increase in action potential frequency and contraction during its contact time. Following removal of the toxin there was a block in responsiveness of the taenia toward 80 mM KCl or acetylcholine whereas the responsiveness toward histamine and BaC1, was only slightly reduced. Partial restoration of the excitability of the preparation was achieved by application of higher calcium concentrations . The effects of the toxin were similar to that of ouabain. On the depolarized preparation the toxin had no effect, the loosely bound fraction of membrane calcium being already released by depolarisation. It is concluded that the toxin may release the loosely bound fraction of membrane calcium. TOXICON1972 Vol. 10.