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Aneurysmal bone cyst-plus lesions (report of three cases)
Poster I / T i s s u e Engineering ~-'~]
ANEURYSMAL BONE CYST-PLUS LESIONS (REPORT OF THREE CASES)
M. Ghazi, B. Rahsepar, B. Zamiri. Shiraz University of Medical Sciences, Iran Aneurysmal bone cyst is an uncommon but well recognized bone lesion. The cause and pathogenesis of ABC of jaws is not known. Several theories have been proposed, but the exact nature of this lesion remains obscure. The association of ABC with other primary bone pathology such as fibrous dysplasia, ossifying fibroma or giant cell tumor has been well documented in medical literature, but not so many in the oral and maxillofacial one. This is a retrospective report of 10 cases of ABC on the Oral Pathology department of Shiraz University of Medical Sciences during 7 years period. Three cases are well documented ABCplus lesions. This study has produced evidence to support the ABC may occur as a secondary phenomenon in a pre-existent lesion. The central giant cell granuloma appears to be particularly predisposed as primary lesion for ABC and occasionally these changes may also occur in other lesions such as fibrous dysplasia and (cemento) ossifying fibroma. The association of the other lesion with ABC can influences the recurrence rate. ~-'~-]
DERMOID CYST - CASE REPORT
L.M. Sassi, J.L. Dissenha, R.L. Simette, L.A. Corbari, G.H.A. Ramos, P.A.G. Pedruzzi, O. Cervantes, B.V. de Oliveira. Hospital Erasto
Gaertner- Liga Paranaense de Combate ao Cancer, PR, Brazil
139 significant change in 1316 genes, with 18 genes being up-regulated, 10 genes being down-regulated and 6 genes being house keeping.The list of these genes includes the categorization of squamous cell carcinoma antigen I, cell cycle, cell proliferation, cell apoptisis, protein metabolism, redox enzyme, transport factor and inflammation. We used immunohistochemistry staining method to identification these proteins and used real time PCR (Polymerase Chain Reaction) to identify the high expression genes. Form Independent-Samples t-test analysis the Col I & Col III over normal people's and OSF Patient's connective tissue had statistically significant difference (P=0.000). From P450 mRNA result, We found the OSF lesions side P450 genes expression less than 4 times for normal tissue and normal side OSF genes expression less than 2 times for normal tissue. For same OSF Patient, we compared the lesion side normal side and using the antibody Col I Col III PDK and P450. The results from Paired-Samples t-test analysis, revealed a statistically significant difference (P=0.015) from P450 antibody in normal side connective, tissue mean ± SD (2.40±0.52) higher than lesion side connective tissue mean ± SD (1.90±0.32). These findings provided genes expression profiled and proteins identification associated with OSF pathogenesis and may provided diagnostic, preventive and therapeutic utilization in OSR
[ - P - ' ~ ] MAXILLARY NECROSIS ASSOCIATED WITH BISPHOSPHONATES. PRESENTATION OF FOUR NEW CASES J. Acero, J. Calvo de Mora, R. Pujol, H. Herencia, I. Garcia Recuero, J.L. Crespo, C. Navarro Vila. Oral and Maxilofacial Surgery Department
Hospital General Universitario Gregorio Mara#6n, Madrid, Spain
Dermoid cyst is one kind of cystic teratoma mainly stemmed from embryonic germinative ephithelium, but in some cases there is also structures from other germinatives layers. In the floor of the mouth, dermoid cysts are rarely found on birth time, most of them occurred in young adults and they don't show gender predominance. Some cysts are just compound for connective tissue's wall lined in its internal surface by fine layer of squamous stratified epithelium, that normally present keratinization, these lesions are appropriated named "epidermic cysts". In other cases several sebaceous glands and pilose follicles as well as sweat glands might be present, these are dermoid cysts that should be surgically removed, however they generally didn't present recurrence. This inflammation can lead to a stimulation and proliferation of embryonic epithelium remnant that can result in a development of cyst structure. It's presented a mandible dermoid cyst and its theraphy. The aim of this study was to raise some data about this pathogeny, which is very rare. It was evaluated the medical records from a patient registered in Erasto Gaertner HospitaI-PR-Brazil. This patient presented osteolytic and well-delimitated images in lower jaw. It was considered clinical and histopathological information as well as the patient treatment and follow up. Case report: Patient C.R.B., female, 25 years old, Caucasian, presented osteolytic painless lesions in mandible. The lesion was identified in a panoramic radiograph that showed an osteolytic image in the region of teeth 36, 37 and 38. The lesion diameter was 3cm approximately. A second lesion was observed in apical region of lower incisors, the diameter was around 2 cm. It was planed a bone biopsy. It was performed bone biopsy in mandible. It was found a bone lodge like a cist, however the substance inside had a "milked" aspect. The histophatological exam revealed a squamous epithelium fragment compatible with epidermoid cyst fragment. It was planned lesions surgical removal, under general anesthesia. It was performed the lesions enucleation. The patient is in follow up for one year, the panoramic radiography present bone neoformation. There was a recurrence of the lesion in lower-anterior region and it was curetted. The patient is in follow up with no clinical recurrence and presenting bone neoformation in radiographic images.
Osteonecrosis of the jaws is an important complication in patients following radiotherapy of the head and neck tumors. Sometimes it's aetiology remains unclear when it appears in patients that are not under such a treatment. Bisphosphonates are synthetic analogues of pyrophosphates and they join to hydroxyapatite inhibiting the osteoclastic action. They are used in postmenopausal women to prevent bone fractures and in myeloma, Paget disease and in tumoral hypercalcemia. In this poster we present a series case of four patients that showed a maxillary necrosis and infection and that were being treated with bisphosphonates due to different reasons. We show the treatment methods we used in the different cases and discuss the evolution in every patient. Maxillary necrosis associated with biphosphonates administration could be a new secondary effect related to this drug. There are still few references regarding this situation in the literature. This is a potential severe complication and should be taken into account in case of a maxillary necrosis of unknown aetiology.
~ - ' ~ - ~ THE MICRO-ARRAY ANALYSIS OF GENETIC CHANGE AND PROTEINS IDENTIFICATION OF ORAL SUBMUCOUS FIBROSIS
The purpose of this study was to develop and characterize the threedimensional culture system of synovium-derived stem cells (SSC) in an atelocollagen scaffold with atelocollagen gel for the use of tissue engineering and/or reproductive medicine. A human synovial membrane from TMJ, knee joint was dissected and digested with 0.2% collagenase at 37°C for 1 hour. The librated cells were expanded in monolayer in DMEM supplemented with 10% FCS. After passages 6, collected cells were suspended and embedded in type 1 atelocollagen solution. Three different mixing methods, which are pippetting, injection of collagen solution, and use of three way stopcock, were tested to make a reproducibly, evenly suspended cell solution. Cell density and distribution of cells embedded in gel were assessed by histological sections stained with
W.B. Kao, "~D. Shieh, Y.J. Hsia, £'~ Shieh. Oral & Maxillofacial Surgery
Department, Kaohsiung Military General Hospital, Kaohsiung, Taiwan From micro-array analysis of genetic change of the oral submucous fibrosis (OSF). We compared the gene expression profile between the normal oral mucosa tissue and OSF tissue, fifty four cases of normal tissue and seventy two cases of clinical diagnosed OSF with evidence of histopthological proof were included in this study. The result revealed
Tissue Engineering
•
DEVELOPMENT AND CHARACTERIZATION OF THREE-DIMENSIONAL CULTURE SYSTEM OF THE SYNOVIUM-DERIVED STEM CELLS IN A COLLAGEN SCAFFOLD WITH COLLAGEN GEL FOR TISSUE ENGINEERING AND REPRODUCTIVE MEDICINE
Y. Muroi 1, K. Nakata 2, N. Nakamura 2, W. Ando2, K. Tateishi 2, H. Yoshikawa 2, K. Kakudo 1. 1Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Japan; 2Department of Orthopedics, Osaka University Med. School, Osaka, Japan
ANEURYSMAL BONE CYST-PLUS LESIONS (REPORT OF THREE CASES)
M. Ghazi, B. Rahsepar, B. Zamiri. Shiraz University of Medical Sciences, Iran Aneurysmal bone cyst is an uncommon but well recognized bone lesion. The cause and pathogenesis of ABC of jaws is not known. Several theories have been proposed, but the exact nature of this lesion remains obscure. The association of ABC with other primary bone pathology such as fibrous dysplasia, ossifying fibroma or giant cell tumor has been well documented in medical literature, but not so many in the oral and maxillofacial one. This is a retrospective report of 10 cases of ABC on the Oral Pathology department of Shiraz University of Medical Sciences during 7 years period. Three cases are well documented ABCplus lesions. This study has produced evidence to support the ABC may occur as a secondary phenomenon in a pre-existent lesion. The central giant cell granuloma appears to be particularly predisposed as primary lesion for ABC and occasionally these changes may also occur in other lesions such as fibrous dysplasia and (cemento) ossifying fibroma. The association of the other lesion with ABC can influences the recurrence rate. ~-'~-]
DERMOID CYST - CASE REPORT
L.M. Sassi, J.L. Dissenha, R.L. Simette, L.A. Corbari, G.H.A. Ramos, P.A.G. Pedruzzi, O. Cervantes, B.V. de Oliveira. Hospital Erasto
Gaertner- Liga Paranaense de Combate ao Cancer, PR, Brazil
139 significant change in 1316 genes, with 18 genes being up-regulated, 10 genes being down-regulated and 6 genes being house keeping.The list of these genes includes the categorization of squamous cell carcinoma antigen I, cell cycle, cell proliferation, cell apoptisis, protein metabolism, redox enzyme, transport factor and inflammation. We used immunohistochemistry staining method to identification these proteins and used real time PCR (Polymerase Chain Reaction) to identify the high expression genes. Form Independent-Samples t-test analysis the Col I & Col III over normal people's and OSF Patient's connective tissue had statistically significant difference (P=0.000). From P450 mRNA result, We found the OSF lesions side P450 genes expression less than 4 times for normal tissue and normal side OSF genes expression less than 2 times for normal tissue. For same OSF Patient, we compared the lesion side normal side and using the antibody Col I Col III PDK and P450. The results from Paired-Samples t-test analysis, revealed a statistically significant difference (P=0.015) from P450 antibody in normal side connective, tissue mean ± SD (2.40±0.52) higher than lesion side connective tissue mean ± SD (1.90±0.32). These findings provided genes expression profiled and proteins identification associated with OSF pathogenesis and may provided diagnostic, preventive and therapeutic utilization in OSR
[ - P - ' ~ ] MAXILLARY NECROSIS ASSOCIATED WITH BISPHOSPHONATES. PRESENTATION OF FOUR NEW CASES J. Acero, J. Calvo de Mora, R. Pujol, H. Herencia, I. Garcia Recuero, J.L. Crespo, C. Navarro Vila. Oral and Maxilofacial Surgery Department
Hospital General Universitario Gregorio Mara#6n, Madrid, Spain
Dermoid cyst is one kind of cystic teratoma mainly stemmed from embryonic germinative ephithelium, but in some cases there is also structures from other germinatives layers. In the floor of the mouth, dermoid cysts are rarely found on birth time, most of them occurred in young adults and they don't show gender predominance. Some cysts are just compound for connective tissue's wall lined in its internal surface by fine layer of squamous stratified epithelium, that normally present keratinization, these lesions are appropriated named "epidermic cysts". In other cases several sebaceous glands and pilose follicles as well as sweat glands might be present, these are dermoid cysts that should be surgically removed, however they generally didn't present recurrence. This inflammation can lead to a stimulation and proliferation of embryonic epithelium remnant that can result in a development of cyst structure. It's presented a mandible dermoid cyst and its theraphy. The aim of this study was to raise some data about this pathogeny, which is very rare. It was evaluated the medical records from a patient registered in Erasto Gaertner HospitaI-PR-Brazil. This patient presented osteolytic and well-delimitated images in lower jaw. It was considered clinical and histopathological information as well as the patient treatment and follow up. Case report: Patient C.R.B., female, 25 years old, Caucasian, presented osteolytic painless lesions in mandible. The lesion was identified in a panoramic radiograph that showed an osteolytic image in the region of teeth 36, 37 and 38. The lesion diameter was 3cm approximately. A second lesion was observed in apical region of lower incisors, the diameter was around 2 cm. It was planed a bone biopsy. It was performed bone biopsy in mandible. It was found a bone lodge like a cist, however the substance inside had a "milked" aspect. The histophatological exam revealed a squamous epithelium fragment compatible with epidermoid cyst fragment. It was planned lesions surgical removal, under general anesthesia. It was performed the lesions enucleation. The patient is in follow up for one year, the panoramic radiography present bone neoformation. There was a recurrence of the lesion in lower-anterior region and it was curetted. The patient is in follow up with no clinical recurrence and presenting bone neoformation in radiographic images.
Osteonecrosis of the jaws is an important complication in patients following radiotherapy of the head and neck tumors. Sometimes it's aetiology remains unclear when it appears in patients that are not under such a treatment. Bisphosphonates are synthetic analogues of pyrophosphates and they join to hydroxyapatite inhibiting the osteoclastic action. They are used in postmenopausal women to prevent bone fractures and in myeloma, Paget disease and in tumoral hypercalcemia. In this poster we present a series case of four patients that showed a maxillary necrosis and infection and that were being treated with bisphosphonates due to different reasons. We show the treatment methods we used in the different cases and discuss the evolution in every patient. Maxillary necrosis associated with biphosphonates administration could be a new secondary effect related to this drug. There are still few references regarding this situation in the literature. This is a potential severe complication and should be taken into account in case of a maxillary necrosis of unknown aetiology.
~ - ' ~ - ~ THE MICRO-ARRAY ANALYSIS OF GENETIC CHANGE AND PROTEINS IDENTIFICATION OF ORAL SUBMUCOUS FIBROSIS
The purpose of this study was to develop and characterize the threedimensional culture system of synovium-derived stem cells (SSC) in an atelocollagen scaffold with atelocollagen gel for the use of tissue engineering and/or reproductive medicine. A human synovial membrane from TMJ, knee joint was dissected and digested with 0.2% collagenase at 37°C for 1 hour. The librated cells were expanded in monolayer in DMEM supplemented with 10% FCS. After passages 6, collected cells were suspended and embedded in type 1 atelocollagen solution. Three different mixing methods, which are pippetting, injection of collagen solution, and use of three way stopcock, were tested to make a reproducibly, evenly suspended cell solution. Cell density and distribution of cells embedded in gel were assessed by histological sections stained with
W.B. Kao, "~D. Shieh, Y.J. Hsia, £'~ Shieh. Oral & Maxillofacial Surgery
Department, Kaohsiung Military General Hospital, Kaohsiung, Taiwan From micro-array analysis of genetic change of the oral submucous fibrosis (OSF). We compared the gene expression profile between the normal oral mucosa tissue and OSF tissue, fifty four cases of normal tissue and seventy two cases of clinical diagnosed OSF with evidence of histopthological proof were included in this study. The result revealed
Tissue Engineering
•
DEVELOPMENT AND CHARACTERIZATION OF THREE-DIMENSIONAL CULTURE SYSTEM OF THE SYNOVIUM-DERIVED STEM CELLS IN A COLLAGEN SCAFFOLD WITH COLLAGEN GEL FOR TISSUE ENGINEERING AND REPRODUCTIVE MEDICINE
Y. Muroi 1, K. Nakata 2, N. Nakamura 2, W. Ando2, K. Tateishi 2, H. Yoshikawa 2, K. Kakudo 1. 1Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, Japan; 2Department of Orthopedics, Osaka University Med. School, Osaka, Japan