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Antioxidant activity of Agaricus blazei
Fitoterapia 78 (2007) 263 – 264 www.elsevier.com/locate/fitote
Short report
Antioxidant activity of Agaricus blazei O.M.M.F. Oliveira a,⁎, J.C.R. Vellosa a,b , A.S. Fernandes a , W. Buffa-Filho c , R.A. Hakime-Silva a , M. Furlan c , I.L. Brunetti b a
Unesp, Sao Paulo State University, Biochemistry and Technology Chemistry Department, Instituto de Química, Araraquara-SP, Brazil b Unesp, Sao Paulo State University, Clinical Analysis Department, Faculdade de Ciências Farmacêuticas, Araraquara-SP, Brazil c Unesp, Sao Paulo State University, Organic Chemistry Department, Instituto de Química, Araraquara-SP, Brazil Received 30 November 2005, accepted 8 January 2007 Available online 6 February 2007
Abstract The ethanolic extract of Agaricus blazei and ethyl acetate and hydroalcoholic fractions were evaluated for their antioxidant activity. © 2007 Elsevier B.V. All rights reserved. Keywords: Agaricus blazei; Antioxidant activity; DPPH radical; ABTS radical
1. Plant A. blazei fruiting bodies, collected on “Estancia Valemar” (Sao Jose do Rio Preto — SP) were identified by Dr. Arailde Fontes Urben (EMBRAPA researcher). 2. Uses in traditional medicine The infusion of the fruiting body has traditionally been used in Brazil for prevention of cancer [1–4], and diabetes, hyperlipidemia, arteriosclerosis and chronic hepatitis activities [5]. 3. Previously isolated classes of constituents Ergosterol [5], polysaccharides [2,6,7] and phenolic compounds [8].
⁎ Corresponding author. UNESP Instituto de Química, Diretoria, R. Prof. Francisco Degni, s/n., Quitandinha, Araraquara-SP, Brazil. Tel.: +55 16 33016680; fax: +55 16 33222308. E-mail addresses: [email protected] (O.M.M.F. Oliveira), [email protected] (A.S. Fernandes). 0367-326X/$ - see front matter © 2007 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2007.01.002
264
O.M.M.F. Oliveira et al. / Fitoterapia 78 (2007) 263–264
Table 1 Scavenger effect of A. blazei ethanol extract, ethyl acetate fraction and hydroalcoholic fraction on DPPH (60 μM) and ABTS•+ (0.1 mM)a Material
DPPH
ABTS•+
Ethanol extract Ethanol fraction Hydroalcoholic fraction Trolox Uric acid
7.9 ± 1.87 7.61 ± 2.81 7.24 ± 1.58 2.53 ± 1.53 n.r.b
22.82 ± 2.40 21.61 ± 1.50 20.73 ± 1.76 0.7 ± 2.17 1.05 ± 1.73
Values expressed as median ± standard deviation. N = 3. Expressed by IC50 values (μg/ml). b n.r. = not reached IC50. a
4. Tested material Ethanol extract (yield: 4.43%) of the dried (40 °C) material and ethylacetate fraction (yield: 2.25%) and hydroxyalcoholic soluble fraction (0.54%). 5. Studied activity Bleaching of the DPPH [9,10] and ABTS•+ radicals [11]. 6. Results Reported in Table 1. 7. Conclusions The ethanolic extract and the ethyl acetate and hydroalcoholic fractions from A. blazei have a powerful antioxidant activity useful for the treatment of various diseases mediated by radicals species. Acknowledgements This work was supported by grants provided by FAPESP. Adriano Silva Fernandes is grateful to CAPES for scholarship. References [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Mizuno T, Inagaki R, Kanno T, Hagiwara T, Nakamura T, Itoh T, et al. Agric Biol Chem 1990;54:2889. Itoh H, Ito H, Amano H, Noda H. Jpn J Pharmacol 1994;66:265. Kawagishi H, Kanao T, Inagaki R, Mituno T, Shimura K, Itoh H, et al. Carbohydr Polym 1990;12:393. Fujimiya Y, Suzuki T, Oshiman K, Kobori H, Moriguchi K, Nakashima H, et al. Cancer Immunol Immunother 1998;46:147. Takaku T, Kimura Y, Okuda H. J Nutr 2001;131:1409. Mizuno M, Morimoto M, Minato K, Tsuchia H. Biosci Biotechnol Biochem 1998;62:434. Hashimoto T, Nonaka Y, Minato K, Kawakami S, Mizuno M, Fukuda I, et al. Biosci Biotechnol Biochem 2002;66:1610. Kasuga A, Aoyagi Y, Sugahara T. J Food Sci 1995;60:1113. Dall'Acqua S, Innocenti G. Fitoterapia 2004;75:592. Chun SS, Vattem DA, Lin YT, Shetty K. Proc Biochem 2005;40:809. Pellegrini N, Re R, Yang M, Evans CR. Methods Enzymol 1998;299:379.
Short report
Antioxidant activity of Agaricus blazei O.M.M.F. Oliveira a,⁎, J.C.R. Vellosa a,b , A.S. Fernandes a , W. Buffa-Filho c , R.A. Hakime-Silva a , M. Furlan c , I.L. Brunetti b a
Unesp, Sao Paulo State University, Biochemistry and Technology Chemistry Department, Instituto de Química, Araraquara-SP, Brazil b Unesp, Sao Paulo State University, Clinical Analysis Department, Faculdade de Ciências Farmacêuticas, Araraquara-SP, Brazil c Unesp, Sao Paulo State University, Organic Chemistry Department, Instituto de Química, Araraquara-SP, Brazil Received 30 November 2005, accepted 8 January 2007 Available online 6 February 2007
Abstract The ethanolic extract of Agaricus blazei and ethyl acetate and hydroalcoholic fractions were evaluated for their antioxidant activity. © 2007 Elsevier B.V. All rights reserved. Keywords: Agaricus blazei; Antioxidant activity; DPPH radical; ABTS radical
1. Plant A. blazei fruiting bodies, collected on “Estancia Valemar” (Sao Jose do Rio Preto — SP) were identified by Dr. Arailde Fontes Urben (EMBRAPA researcher). 2. Uses in traditional medicine The infusion of the fruiting body has traditionally been used in Brazil for prevention of cancer [1–4], and diabetes, hyperlipidemia, arteriosclerosis and chronic hepatitis activities [5]. 3. Previously isolated classes of constituents Ergosterol [5], polysaccharides [2,6,7] and phenolic compounds [8].
⁎ Corresponding author. UNESP Instituto de Química, Diretoria, R. Prof. Francisco Degni, s/n., Quitandinha, Araraquara-SP, Brazil. Tel.: +55 16 33016680; fax: +55 16 33222308. E-mail addresses: [email protected] (O.M.M.F. Oliveira), [email protected] (A.S. Fernandes). 0367-326X/$ - see front matter © 2007 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2007.01.002
264
O.M.M.F. Oliveira et al. / Fitoterapia 78 (2007) 263–264
Table 1 Scavenger effect of A. blazei ethanol extract, ethyl acetate fraction and hydroalcoholic fraction on DPPH (60 μM) and ABTS•+ (0.1 mM)a Material
DPPH
ABTS•+
Ethanol extract Ethanol fraction Hydroalcoholic fraction Trolox Uric acid
7.9 ± 1.87 7.61 ± 2.81 7.24 ± 1.58 2.53 ± 1.53 n.r.b
22.82 ± 2.40 21.61 ± 1.50 20.73 ± 1.76 0.7 ± 2.17 1.05 ± 1.73
Values expressed as median ± standard deviation. N = 3. Expressed by IC50 values (μg/ml). b n.r. = not reached IC50. a
4. Tested material Ethanol extract (yield: 4.43%) of the dried (40 °C) material and ethylacetate fraction (yield: 2.25%) and hydroxyalcoholic soluble fraction (0.54%). 5. Studied activity Bleaching of the DPPH [9,10] and ABTS•+ radicals [11]. 6. Results Reported in Table 1. 7. Conclusions The ethanolic extract and the ethyl acetate and hydroalcoholic fractions from A. blazei have a powerful antioxidant activity useful for the treatment of various diseases mediated by radicals species. Acknowledgements This work was supported by grants provided by FAPESP. Adriano Silva Fernandes is grateful to CAPES for scholarship. References [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Mizuno T, Inagaki R, Kanno T, Hagiwara T, Nakamura T, Itoh T, et al. Agric Biol Chem 1990;54:2889. Itoh H, Ito H, Amano H, Noda H. Jpn J Pharmacol 1994;66:265. Kawagishi H, Kanao T, Inagaki R, Mituno T, Shimura K, Itoh H, et al. Carbohydr Polym 1990;12:393. Fujimiya Y, Suzuki T, Oshiman K, Kobori H, Moriguchi K, Nakashima H, et al. Cancer Immunol Immunother 1998;46:147. Takaku T, Kimura Y, Okuda H. J Nutr 2001;131:1409. Mizuno M, Morimoto M, Minato K, Tsuchia H. Biosci Biotechnol Biochem 1998;62:434. Hashimoto T, Nonaka Y, Minato K, Kawakami S, Mizuno M, Fukuda I, et al. Biosci Biotechnol Biochem 2002;66:1610. Kasuga A, Aoyagi Y, Sugahara T. J Food Sci 1995;60:1113. Dall'Acqua S, Innocenti G. Fitoterapia 2004;75:592. Chun SS, Vattem DA, Lin YT, Shetty K. Proc Biochem 2005;40:809. Pellegrini N, Re R, Yang M, Evans CR. Methods Enzymol 1998;299:379.