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ASSOCIATION OF ADIPONECTIN RECEPTORS EXPRESSION ON PERIPHERAL BLOOD MONONUCLEAR CELLS WITH CORONARY PLAQUE VULNERABILITY
E939 JACC April 5, 2011 Volume 57, Issue 14
MYOCARDIAL ISCHEMIA AND INFARCTION ASSOCIATION OF ADIPONECTIN RECEPTORS EXPRESSION ON PERIPHERAL BLOOD MONONUCLEAR CELLS WITH CORONARY PLAQUE VULNERABILITY ACC Poster Contributions Ernest N. Morial Convention Center, Hall F Sunday, April 03, 2011, 10:00 a.m.-11:15 a.m.
Session Title: Unstable Ischemic Syndrome -- Clinical: Novel Markers and Therapies Abstract Category: 2. Unstable Ischemic Syndrome—Clinical Session-Poster Board Number: 1003-374 Authors: Toru Geshi, Hiroyasu Uzui, Akira Nakano, Yasuhiko Mitsuke, Jong-Dae Lee, University of Fukui, Fukui, Japan Background: Adiponectin is an adipose tissue secreted protein known for its insulin sensitising and anti-atherogenic actions. Recently, two adiponectin receptors, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2), have been discovered. The aim of this study was to investigate the association of adiponectin receptors expression on peripheral blood mononuclear cells (PBMNCs) with coronary plaque components in patients with coronary artery disease (CAD). Methods: Twenty-five CAD patients (mean 68.7 years, 16 men) undergoing percutaneous coronary intervention (PCI) were enrolled in this study. Expression of AdipoR1 and AdipoR2 in circulating CD14+ monocytes were determined using flow cytometry. High molecular weight adiponectin (HMW-APN) was measured by enzyme-linked immunosorbent assay. Virtual histology intravascular ultrasound imaging was performed before PCI to assess coronary plaque components in the culprit lesion. Results: Frequencies of CD14+/AdipoR1+ and CD14+/AdipoR2+ monocytes were significantly lower in patients with acute coronary syndrome (ACS) (n = 8) than without ACS (n = 17), respectively (AdipoR1+; 2.25 ± 1.21 % vs. 4.85 ± 3.09 %, p = 0.0326, AdipoR2+; 2.07 ± 1.82 % vs. 3.63 ± 2.75 %, p = 0.0441), whereas plasma HMW-APN levels did not differ between the two groups. The expression levels of AdipoR1 on PBMNCs were inversely correlated with the percentage of fibro-fatty and necrotic core volume (r = -0.527, p = 0.0068), the ratio of necrotic core to dense calcium (r = -0.406, p = 0.044) and the remodeling index (r = -0.384, p = 0.0859). Plasma HMW-APN levels did not correlated with the expression levels of adiponectin receptors on monocytes. Conclusions: Down-regulated adiponectin receptors, especially AdipoR1, on PBMNCs were associated with coronary plaque vulnerability, suggesting that adiponectin sensitivity in PBMNCs might be a surrogate marker for stratifying “vulnerable patients”.
MYOCARDIAL ISCHEMIA AND INFARCTION ASSOCIATION OF ADIPONECTIN RECEPTORS EXPRESSION ON PERIPHERAL BLOOD MONONUCLEAR CELLS WITH CORONARY PLAQUE VULNERABILITY ACC Poster Contributions Ernest N. Morial Convention Center, Hall F Sunday, April 03, 2011, 10:00 a.m.-11:15 a.m.
Session Title: Unstable Ischemic Syndrome -- Clinical: Novel Markers and Therapies Abstract Category: 2. Unstable Ischemic Syndrome—Clinical Session-Poster Board Number: 1003-374 Authors: Toru Geshi, Hiroyasu Uzui, Akira Nakano, Yasuhiko Mitsuke, Jong-Dae Lee, University of Fukui, Fukui, Japan Background: Adiponectin is an adipose tissue secreted protein known for its insulin sensitising and anti-atherogenic actions. Recently, two adiponectin receptors, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2), have been discovered. The aim of this study was to investigate the association of adiponectin receptors expression on peripheral blood mononuclear cells (PBMNCs) with coronary plaque components in patients with coronary artery disease (CAD). Methods: Twenty-five CAD patients (mean 68.7 years, 16 men) undergoing percutaneous coronary intervention (PCI) were enrolled in this study. Expression of AdipoR1 and AdipoR2 in circulating CD14+ monocytes were determined using flow cytometry. High molecular weight adiponectin (HMW-APN) was measured by enzyme-linked immunosorbent assay. Virtual histology intravascular ultrasound imaging was performed before PCI to assess coronary plaque components in the culprit lesion. Results: Frequencies of CD14+/AdipoR1+ and CD14+/AdipoR2+ monocytes were significantly lower in patients with acute coronary syndrome (ACS) (n = 8) than without ACS (n = 17), respectively (AdipoR1+; 2.25 ± 1.21 % vs. 4.85 ± 3.09 %, p = 0.0326, AdipoR2+; 2.07 ± 1.82 % vs. 3.63 ± 2.75 %, p = 0.0441), whereas plasma HMW-APN levels did not differ between the two groups. The expression levels of AdipoR1 on PBMNCs were inversely correlated with the percentage of fibro-fatty and necrotic core volume (r = -0.527, p = 0.0068), the ratio of necrotic core to dense calcium (r = -0.406, p = 0.044) and the remodeling index (r = -0.384, p = 0.0859). Plasma HMW-APN levels did not correlated with the expression levels of adiponectin receptors on monocytes. Conclusions: Down-regulated adiponectin receptors, especially AdipoR1, on PBMNCs were associated with coronary plaque vulnerability, suggesting that adiponectin sensitivity in PBMNCs might be a surrogate marker for stratifying “vulnerable patients”.