- Email: [email protected]
Autoantibody specificities in the human early B cell repertoire
610
Abstracts
diseaee eeverity or other clinical or immunological disease to manifeetations. These patient6 also fulfilled a larger average number of ARA criteria. Patients with a history of thyroid disease also had significantly lower values. In contrast, patients with >20 mm on Schirmer'e test had a lower incidence of disease flares and wers taking lower mean prednieolone dosage, suggesting mild disease. Serositis was less common and discoid lupus more common in this group. Sequential tests in individual patients showed great variations in Schinner's test with time, and related to disease flares and treatment. Reversible impairment of glandular functions seams to be common in active SLE.
ANTIKDIESAND~YlHFMA IN PATIEN'IS RELATICi$HIP BE?wEENANII-Ro/SSAAND/oRANII-La/SSH WIlHSJoGqI'ssyNcRcME. H.Yamagata,_T.'JXojo, Y.Ckano,M.Heyakawati M.HomM, NationalMurayamsHospital*and Keio University,'Itokyo (Japan) As sane kind of erythemnsare found in association with anti-Ia/SSH and/orantiRo/SSAanti~iesinpatientswith SLZ (anti-Raq-draws) and the SimilarerythgMs are also found in Sjb;gren's SW (SjS),relationship betweenerythenmand the presence of these two antibodieswere examined,154 patientswith SjS were diagnosedby the presenceof at leastone of the follcwingitemswith subjectivesioca symptans;keratooonjunotivitis sioca,xerostaniaand laxer lip biopsy. Patientswitn S&E were used as a controlwho fulfilledthe difinitecriteriaby AmericanPhemtisn Association.AntiI.~/sSH axI anti-Ro/SSAantibodieswere deteotedby double inrmmodiffusion tests using rabbitthymusextractand calf spleenextractas the antigens. Inoidenceof non-scaring eqthems was higherin patientswith SjS (22.7%)than those with SLS (19.7%),and incidence of antibadiesamong patientswith eqthems were as follows;in SjS, anti-Ia/SSH(+) ard anti-Ro/SSA(+) 51.4%,anti-Ia/SSH(-) and anti-Ro/SSA(+) 37.1%,both negative11.4% while in SLR, 4.2%, 59.5%ti 36.7% respeotively.Widespreadannularery+&mawasonly fcn.uxl inSjSwithanti-Ia/SSHantibody. Hydivisicnof SjSintoprismryand secondary Sjs (mainlywithSLR),indFoenoeofsqthemawashigherin seccn&q SjS (32.1%). F'urther classification with the specificities of their antibodiesdisclosedhigherinoidence of erytlmmin patientswith secondarySjS arxlwith anti-Ia/SSH(g/14,64.3%). Howaver, widespread annulareqthemawas fcund inbothprimary (3 patients)arKIseconeqihenmwith theantibodies dary SjS (4). Thesedata suggestedcloeeassociationof espeoiallywithanti-Ia/SSH, whatever the diagnosis is andalsoirdioated the anmcn pathogenesis under the abnarmalities.
AUTOANTIBODY SPECIFICITIES IN THE HUMAN EARLY B CELL REPERTOIRE. PM Lydyard, L Mackenzie, DI Isenberg et P Youinou. The Middlesex Hospital Medical School, London UK, and Brest University Medical School, Brest FRANCE. The number of CD5-positive-B cells is significantly higher in patients with Sjdgren’s syndrome than in normal. In order to analyse the autoreactivity repertoire of cord blood and foetal liver B cells at the cellular level, we used the polyclonal B cell activator Epstein-Barr virus to produce lines and clones. Umbilical cord blood from (CB) 7 donors and one foetal liver cells were used in the study. Antibody assays included IgM rheumatoid factor (RF), IgM antibodies to ss and ds DNA, collagen types I and II, thyroglobulin, cytoskeleton. The 16/6 idiotype and PR4 were detected by direct and blocking assays. Of 100 IgM-containing CB supernatants tested, all but 16 bound to one or a number of different autoantigens and similar findings were obtained with the putative clones. Some IgM supernatants bound also to microorganisms (various bacteria and candidal. Four lines were positive for the 16/6 idiotype in the direct and competition assays. Addition of the 4 Ig M supernatants resulted in a dose-dependent inhibition of binding of the anti-16/6 antibodies. None of the supernatants were positive for the PR4 idiotype.
IS THERE A PARTICULAR TARGET FOR ANTI-COLGI COMPLEXAUTOANTIBODIES IN PATIENTSWITH PRIMARY SSOGREN’S SYNDROME? MA Blaschek, YL Pennec and P Youinou, Brest University Medical School, Erest (FRANCE) Tests for anti-Golgi complex (AGC) antibodies were performed on 393 disease-associated and normal control sera by indirect irmnunofluorescence on gerbil fibrosarcoma, 373 and HepZ cells. The incidence was significantly raised (37.5 versus 9.1 % and versus
Abstracts
diseaee eeverity or other clinical or immunological disease to manifeetations. These patient6 also fulfilled a larger average number of ARA criteria. Patients with a history of thyroid disease also had significantly lower values. In contrast, patients with >20 mm on Schirmer'e test had a lower incidence of disease flares and wers taking lower mean prednieolone dosage, suggesting mild disease. Serositis was less common and discoid lupus more common in this group. Sequential tests in individual patients showed great variations in Schinner's test with time, and related to disease flares and treatment. Reversible impairment of glandular functions seams to be common in active SLE.
ANTIKDIESAND~YlHFMA IN PATIEN'IS RELATICi$HIP BE?wEENANII-Ro/SSAAND/oRANII-La/SSH WIlHSJoGqI'ssyNcRcME. H.Yamagata,_T.'JXojo, Y.Ckano,M.Heyakawati M.HomM, NationalMurayamsHospital*and Keio University,'Itokyo (Japan) As sane kind of erythemnsare found in association with anti-Ia/SSH and/orantiRo/SSAanti~iesinpatientswith SLZ (anti-Raq-draws) and the SimilarerythgMs are also found in Sjb;gren's SW (SjS),relationship betweenerythenmand the presence of these two antibodieswere examined,154 patientswith SjS were diagnosedby the presenceof at leastone of the follcwingitemswith subjectivesioca symptans;keratooonjunotivitis sioca,xerostaniaand laxer lip biopsy. Patientswitn S&E were used as a controlwho fulfilledthe difinitecriteriaby AmericanPhemtisn Association.AntiI.~/sSH axI anti-Ro/SSAantibodieswere deteotedby double inrmmodiffusion tests using rabbitthymusextractand calf spleenextractas the antigens. Inoidenceof non-scaring eqthems was higherin patientswith SjS (22.7%)than those with SLS (19.7%),and incidence of antibadiesamong patientswith eqthems were as follows;in SjS, anti-Ia/SSH(+) ard anti-Ro/SSA(+) 51.4%,anti-Ia/SSH(-) and anti-Ro/SSA(+) 37.1%,both negative11.4% while in SLR, 4.2%, 59.5%ti 36.7% respeotively.Widespreadannularery+&mawasonly fcn.uxl inSjSwithanti-Ia/SSHantibody. Hydivisicnof SjSintoprismryand secondary Sjs (mainlywithSLR),indFoenoeofsqthemawashigherin seccn&q SjS (32.1%). F'urther classification with the specificities of their antibodiesdisclosedhigherinoidence of erytlmmin patientswith secondarySjS arxlwith anti-Ia/SSH(g/14,64.3%). Howaver, widespread annulareqthemawas fcund inbothprimary (3 patients)arKIseconeqihenmwith theantibodies dary SjS (4). Thesedata suggestedcloeeassociationof espeoiallywithanti-Ia/SSH, whatever the diagnosis is andalsoirdioated the anmcn pathogenesis under the abnarmalities.
AUTOANTIBODY SPECIFICITIES IN THE HUMAN EARLY B CELL REPERTOIRE. PM Lydyard, L Mackenzie, DI Isenberg et P Youinou. The Middlesex Hospital Medical School, London UK, and Brest University Medical School, Brest FRANCE. The number of CD5-positive-B cells is significantly higher in patients with Sjdgren’s syndrome than in normal. In order to analyse the autoreactivity repertoire of cord blood and foetal liver B cells at the cellular level, we used the polyclonal B cell activator Epstein-Barr virus to produce lines and clones. Umbilical cord blood from (CB) 7 donors and one foetal liver cells were used in the study. Antibody assays included IgM rheumatoid factor (RF), IgM antibodies to ss and ds DNA, collagen types I and II, thyroglobulin, cytoskeleton. The 16/6 idiotype and PR4 were detected by direct and blocking assays. Of 100 IgM-containing CB supernatants tested, all but 16 bound to one or a number of different autoantigens and similar findings were obtained with the putative clones. Some IgM supernatants bound also to microorganisms (various bacteria and candidal. Four lines were positive for the 16/6 idiotype in the direct and competition assays. Addition of the 4 Ig M supernatants resulted in a dose-dependent inhibition of binding of the anti-16/6 antibodies. None of the supernatants were positive for the PR4 idiotype.
IS THERE A PARTICULAR TARGET FOR ANTI-COLGI COMPLEXAUTOANTIBODIES IN PATIENTSWITH PRIMARY SSOGREN’S SYNDROME? MA Blaschek, YL Pennec and P Youinou, Brest University Medical School, Erest (FRANCE) Tests for anti-Golgi complex (AGC) antibodies were performed on 393 disease-associated and normal control sera by indirect irmnunofluorescence on gerbil fibrosarcoma, 373 and HepZ cells. The incidence was significantly raised (37.5 versus 9.1 % and versus