- Email: [email protected]
Bioremediation of TBT-contaminated marine waters by bacteria and ecotoxicological evaluation of its by-products
S38
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
OP17
OP19
Evaluation of pH, ORP and conductivity profiles in an Anammox reactor started-up using municipal activated sludge seed
Bioremediation of TBT-contaminated marine waters by bacteria and ecotoxicological evaluation of its byproducts
Bilge Alpaslan Kocamemi, Duygu Dityapak, Neslihan Semerci
Hugo R Monteiro 1 , Ana C Esteves 2 , António Correia 2 , Amadeu MVM Soares 2 , Marco FL Lemos 3
Department of Environmental Engineering, Marmara University, Istanbul, Turkey
1
ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal CESAM and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal 3 ESTM and GIRM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal 2
E-mail address: [email protected] (B.A. Kocamemi) Similar to the other biological processes, on-line monitoring of Anammox process through real-time measurement of process constituents (NH4 + , NO2 − , NO3 − and N2 ) is very difficult and costly. But, the process dynamics can easily and cheaply be monitored by real-time pH, ORP and conductivity measurements if the process is in a way correlated to profiles of pH, ORP and conductivity. This study evaluates the possible use of pH, ORP and conductivity profiles to monitor and control Anammox process. An SBR system was started-up by seeding with municipal activated sludge and operated for 240 days via stepwise loading increase. pH, ORP and conductivity values were continuously monitored and recorded. pH, ORP and conductivity profiles were well related to the process. With the beginning of degradation, pH exhibited an increasing trend while the conductivity and ORP decreased gradually. With NO3 production, conductivity values remained almost constant while the ORP values increased gradually. At the end of the process, a slight decrease in pH values were observed. The findings demonstrated that the status of Anammox process in a bioreactor can successfully be monitored and controlled through pH, ORP and conductivity profiles. doi:10.1016/j.copbio.2011.05.088
OP18 Influence of thermal pretreatment and pH in eucalypt sulphite spent liquor as substrate fermentation by Pichia stipitis and Pachysolen tannophilus Abstract not supplied. doi:10.1016/j.copbio.2011.05.089
E-mail address: [email protected] (H.R. Monteiro) Tributyltin (TBT) is an organotin commonly used as an antifouling agent in marine paint formulations. Due to its wide industrial application and its consequent discharge into the environment, TBT pollution is recognized as major environmental problem at a global scale. Microorganisms from historically contaminated sites are due to tolerate pollutants and even degrade them, which is a key factor in the restoration of contaminated environments. Nevertheless, byproducts resulting from the degradation process might be more or less toxic than the parent compound. The determination of the substance presence by analytical chemistry, although essential, may not present ecological relevance as it might not be related to its ecotoxicity. In this study, TBT-resistant bacteria collected from 7 Portuguese ports were isolated in increasing concentrations of the toxicant (0.1, 1, and 3 mM of TBT) and those growing at the highest concentration were characterized by REP-PCR and tested as potential bioremediation tool in laboratory contaminated media. The potential of these selected microorganisms to bioremediate waters was assessed through ecotoxicological testing with the amphipod Echinogammarus marinus. The concentration of TBT resistant isolates varied between 0.08% (in Setúbal harbor) and 7.67% (Peniche) and differ in diversity, offering a wide application in TBT remediation. doi:10.1016/j.copbio.2011.05.090
OP20 Computer-assisted enzyme engineering by modification of tunnels, channels and gates Artur Gora, Jan Brezovsky, Jiri Damborsky Loschmidt Laboratories, Masaryk University, Czech Republic E-mail address: [email protected] (A. Gora) Enzymes are natural biocatalysts evolved for high selectivity and activity towards wide range of substrates. Protein engineering makes a use of the knowledge gained from protein structure/reactivity/selectivity relationships studies for the construction of improved biocatalysts for practical applications. Continuous efforts in protein engineering projects resulted in hundreds of successful constructs with higher activity and selectivity, better protein stability or broadened substrate specificity. Modifications in the residues laying the first or second shell of the active site are currently the most commonly used for rational engineering of enzymes. Here we present several success stories on enzyme redesigns targeting the residues making-up the tunnels, channels and gates. Enzyme engineering focused on these structural futures is a new strategy for efficient control of enzyme properties. These examples allow us to understand the principles of the control of ligand transport, reaction coupling and other pre-biocatalytic
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
OP17
OP19
Evaluation of pH, ORP and conductivity profiles in an Anammox reactor started-up using municipal activated sludge seed
Bioremediation of TBT-contaminated marine waters by bacteria and ecotoxicological evaluation of its byproducts
Bilge Alpaslan Kocamemi, Duygu Dityapak, Neslihan Semerci
Hugo R Monteiro 1 , Ana C Esteves 2 , António Correia 2 , Amadeu MVM Soares 2 , Marco FL Lemos 3
Department of Environmental Engineering, Marmara University, Istanbul, Turkey
1
ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal CESAM and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal 3 ESTM and GIRM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal 2
E-mail address: [email protected] (B.A. Kocamemi) Similar to the other biological processes, on-line monitoring of Anammox process through real-time measurement of process constituents (NH4 + , NO2 − , NO3 − and N2 ) is very difficult and costly. But, the process dynamics can easily and cheaply be monitored by real-time pH, ORP and conductivity measurements if the process is in a way correlated to profiles of pH, ORP and conductivity. This study evaluates the possible use of pH, ORP and conductivity profiles to monitor and control Anammox process. An SBR system was started-up by seeding with municipal activated sludge and operated for 240 days via stepwise loading increase. pH, ORP and conductivity values were continuously monitored and recorded. pH, ORP and conductivity profiles were well related to the process. With the beginning of degradation, pH exhibited an increasing trend while the conductivity and ORP decreased gradually. With NO3 production, conductivity values remained almost constant while the ORP values increased gradually. At the end of the process, a slight decrease in pH values were observed. The findings demonstrated that the status of Anammox process in a bioreactor can successfully be monitored and controlled through pH, ORP and conductivity profiles. doi:10.1016/j.copbio.2011.05.088
OP18 Influence of thermal pretreatment and pH in eucalypt sulphite spent liquor as substrate fermentation by Pichia stipitis and Pachysolen tannophilus Abstract not supplied. doi:10.1016/j.copbio.2011.05.089
E-mail address: [email protected] (H.R. Monteiro) Tributyltin (TBT) is an organotin commonly used as an antifouling agent in marine paint formulations. Due to its wide industrial application and its consequent discharge into the environment, TBT pollution is recognized as major environmental problem at a global scale. Microorganisms from historically contaminated sites are due to tolerate pollutants and even degrade them, which is a key factor in the restoration of contaminated environments. Nevertheless, byproducts resulting from the degradation process might be more or less toxic than the parent compound. The determination of the substance presence by analytical chemistry, although essential, may not present ecological relevance as it might not be related to its ecotoxicity. In this study, TBT-resistant bacteria collected from 7 Portuguese ports were isolated in increasing concentrations of the toxicant (0.1, 1, and 3 mM of TBT) and those growing at the highest concentration were characterized by REP-PCR and tested as potential bioremediation tool in laboratory contaminated media. The potential of these selected microorganisms to bioremediate waters was assessed through ecotoxicological testing with the amphipod Echinogammarus marinus. The concentration of TBT resistant isolates varied between 0.08% (in Setúbal harbor) and 7.67% (Peniche) and differ in diversity, offering a wide application in TBT remediation. doi:10.1016/j.copbio.2011.05.090
OP20 Computer-assisted enzyme engineering by modification of tunnels, channels and gates Artur Gora, Jan Brezovsky, Jiri Damborsky Loschmidt Laboratories, Masaryk University, Czech Republic E-mail address: [email protected] (A. Gora) Enzymes are natural biocatalysts evolved for high selectivity and activity towards wide range of substrates. Protein engineering makes a use of the knowledge gained from protein structure/reactivity/selectivity relationships studies for the construction of improved biocatalysts for practical applications. Continuous efforts in protein engineering projects resulted in hundreds of successful constructs with higher activity and selectivity, better protein stability or broadened substrate specificity. Modifications in the residues laying the first or second shell of the active site are currently the most commonly used for rational engineering of enzymes. Here we present several success stories on enzyme redesigns targeting the residues making-up the tunnels, channels and gates. Enzyme engineering focused on these structural futures is a new strategy for efficient control of enzyme properties. These examples allow us to understand the principles of the control of ligand transport, reaction coupling and other pre-biocatalytic