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C198 EXPRESSION OF IGFBP3 DIFFERS CLEAR CELL RENAL CELL CARCINOMA FROM OTHER KIDNEY CANCER
C198
Expression of IGFBP3 differs clear cell renal cell carcinoma from other kidney cancer
Braczkowski R.S.1, Białożyt M.2, Duda W.2 Silesian Medical University, Dept. of Public Health, Bytom, Poland, 2Prof. E. Michalowski Hospital, Dept. of Urology, Katowice, Poland
1
Introduction & Objectives: According to recent data number of cases of kidney cancer rising steadily and represents 3-4% all human malignant neoplasms. 3 766 new cases of kidney cancer have been noted in Poland in 2006. 64 770 cases of kidney and renal pelvis cancer is estimated in the USA for 2012 Renal cell carcinoma is the most common type of kidney cancer in adults, it is responsible for approximately 80% of cases. Clear cell renal cell carcinoma is a distinct and the most frequent subtype of renal cell carcinoma.Insulin like growth factors I and II (IGFs) are peptides with strong promitotic and antiapoptotic effect. The action of both is mediated through the activation of IGF –I receptor (IGF-IR) a tetrameric transmembrane receptor with tyrosine kinase activity and in a small part through the activation of insulin receptor.Activity of IGFs is regulating by their binding proteins (IGFBP) – most important of which is IGFBP-3. There are number of evidence indicative that bindig IGFs ligands and subsequent activation of IGF-IR can promote growth of many cancersIGFs have strong mitogenic and antiapoptopic effects on normal and transformed kidney cell. All these facts suggests that IGFs may promote the development and growth of kidny cancers. To find the differences in the presence of IGFs, their receptors and IGFBP-3 gene expression between renal cell carcinoma and other kidney cancers is the aim of ou study. Materials & Methods: 62 patients qualified to radical nephrectomy because o kidney cancer, in age 25 – 65 were included to the study. After histophological examination 2 groups has been separated. 1. - ca clarocellulare (50) and 2. others (12 - this group includes: angiolipoma, ca papillare, ca urotheliale). Expression of genes for IGFs, IGF -1 receptor and IGFBP-3 were evaluated by QRT-PCR. Statistical analyses were performed by using STATISTICA 8 software. Results: There wewre no statistically significant differences in expression of IGF-1, IGF-2 and IGF-1R, gene expression between group 1 and 2. Expression of IGFBP3 gene in clear renal cell carcinoma specimens is higher than in other renal cancers. Conclusions: Expression of IGFBP3 gene in clear renal cell carcinoma is higher in clear renal cell carcinoma than in other kidney cancers.
Eur Urol Suppl 2012;11(4):130
Expression of IGFBP3 differs clear cell renal cell carcinoma from other kidney cancer
Braczkowski R.S.1, Białożyt M.2, Duda W.2 Silesian Medical University, Dept. of Public Health, Bytom, Poland, 2Prof. E. Michalowski Hospital, Dept. of Urology, Katowice, Poland
1
Introduction & Objectives: According to recent data number of cases of kidney cancer rising steadily and represents 3-4% all human malignant neoplasms. 3 766 new cases of kidney cancer have been noted in Poland in 2006. 64 770 cases of kidney and renal pelvis cancer is estimated in the USA for 2012 Renal cell carcinoma is the most common type of kidney cancer in adults, it is responsible for approximately 80% of cases. Clear cell renal cell carcinoma is a distinct and the most frequent subtype of renal cell carcinoma.Insulin like growth factors I and II (IGFs) are peptides with strong promitotic and antiapoptotic effect. The action of both is mediated through the activation of IGF –I receptor (IGF-IR) a tetrameric transmembrane receptor with tyrosine kinase activity and in a small part through the activation of insulin receptor.Activity of IGFs is regulating by their binding proteins (IGFBP) – most important of which is IGFBP-3. There are number of evidence indicative that bindig IGFs ligands and subsequent activation of IGF-IR can promote growth of many cancersIGFs have strong mitogenic and antiapoptopic effects on normal and transformed kidney cell. All these facts suggests that IGFs may promote the development and growth of kidny cancers. To find the differences in the presence of IGFs, their receptors and IGFBP-3 gene expression between renal cell carcinoma and other kidney cancers is the aim of ou study. Materials & Methods: 62 patients qualified to radical nephrectomy because o kidney cancer, in age 25 – 65 were included to the study. After histophological examination 2 groups has been separated. 1. - ca clarocellulare (50) and 2. others (12 - this group includes: angiolipoma, ca papillare, ca urotheliale). Expression of genes for IGFs, IGF -1 receptor and IGFBP-3 were evaluated by QRT-PCR. Statistical analyses were performed by using STATISTICA 8 software. Results: There wewre no statistically significant differences in expression of IGF-1, IGF-2 and IGF-1R, gene expression between group 1 and 2. Expression of IGFBP3 gene in clear renal cell carcinoma specimens is higher than in other renal cancers. Conclusions: Expression of IGFBP3 gene in clear renal cell carcinoma is higher in clear renal cell carcinoma than in other kidney cancers.
Eur Urol Suppl 2012;11(4):130