- Email: [email protected]
Circulating tumor cells (CTCs) in association to breast cancer subtypes and inflammatory pattern
S98
Abstracts / Journal of Biotechnology 185S (2014) S37–S125
acid (C22:63, DHA), play multifunctional roles in human health and diseases. The traditional source of these fatty acids is fish. Since fish stocks are in global decline, new sources of omega-3 LC-PUFA have to be found. The aim of the present study was to find potential alternative sources of these fatty acids in the territory of The Republic of Kazakhstan. Twelve strains of filamentous fungi isolated from soil and water were screened for lipids and LCPUFA: Penicillium sp., Penicillium restrictum, Penicillium aculeatum, Penicillium anatolicum, Penicillium bilaiae, Penicillium commune, Penicillium raistrikii, Penicillium crustosum, Fusarium sp. (2 strains), Aspergillus sp. and Mucor circinelloides. These fungi were grown in Sabouraud medium for 10 days. Lipid contents of these strains ranged from 1.5% to 7.4% of dry weight. No LC-PUFA were found in P. commune, P. crustosum, Aspergillus sp., M. circinelloides, whereas other strains contained EPA ranging from 1.0% to 3.5% of the total fatty acids, P. restrictum accumulated the largest amount. The cultural conditions that can increase the production of EPA in P. restrictum (e.g. the addition of glucose, glycerin and zinc) are currently under investigation. http://dx.doi.org/10.1016/j.jbiotec.2014.07.332 Circulating tumor cells (CTCs) in association to breast cancer subtypes and inflammatory pattern Angela Toss 1,∗ , Jocelyn A. Sendecki 2 , Tiffany P. Avery 2 , James M. Reuben 3 , Massimo Cristofanilli 2 1 University of Modena and Reggio Emilia, Modena, Italy 2 Thomas Jefferson University, Philadelphia, PA, USA 3 The University of Texas MD Anderson Cancer Center, Huston, TX, USA
The phytochemical, antibacterial and antioxidant activity of wild plants growing in Kazakhstan against diabetic foot ulcer Tatyana Kustova 1,∗ , Tatyana Karpenyuk 1 , Alla Goncharova 1 , Samir Ross 2 1 Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan 2 School of Pharmacy, NCNPR, The University of Mississippi, University, USA
E-mail address: kus [email protected] (T. Kustova). Diabetic foot infection (DFI) is one of the most frequent and complex problems among patients suffering from diabetes. DFI is polymicrobial in nature. Moreover most of the bacteria have multidrug resistance. Therefore, much attention has been paid to find biological active substances from plants that help to solve this problem. Thus, the aim of this study was to evaluate phytochemical, antibacterial and antioxidant activity of crude extracts isolated from plants growing in Kazakhstan: Epilobium hirsutum L. (Onagraceae), Rumex confertus Wiild. (Polygonaceae), Vexibia alopecuroides (L.) Jakovl. (Fabaceae). The results of the present research clearly indicate that the antibacterial and antioxidant activities vary with the plant species. Among the different crude extract that were evaluated, the dichloromethane extract of roots of V. alopecuroides showed the strongest activity against Staphylococcus aureus (IC50 3.05 g/mL) and methicillin-resistant S. aureus (IC50 2.9 g/mL). Despite the fact that the extract of V. alopecuroides has not shown high antioxidant activity, it is still highly promising for the study because it showed high antimicrobial activity. Highest antioxidant activity (<85% inhibition of DPPH) was shown by E. hirsutum L. and R. confertus Wiild. Based on the research results plants were selected to produce drugs from their extracts. http://dx.doi.org/10.1016/j.jbiotec.2014.07.334
E-mail address: [email protected] (A. Toss). Circulating tumor cells (CTCs) are an independent prognostic factor for progression-free (PFS) and overall survival (OS) in metastatic breast cancer (MBC). The present analysis evaluates the prognostic value of CTCs in HER-2 positive disease receiving targeted therapies. This is a retrospective analysis of 516 patients with MBC from the MDACC database. The PFS and OS were evaluated in association to baseline CTCs (<5 vs. ≥5) within the different subtypes (HR+/HER2−, HR+/HER2+, HR−/HER2+, TNBC) and inflammatory breast cancer (IBC). Among 516 patients, 56% had HR+/HER2− tumor, 10% HR+/HER2+, 10% HR−/HER2+, and 24% were TNBC, including 25% with IBC. Overall, patients with CTCs ≥ 5 had a significantly lower median survival time (HR = 2.00). In TNBC, HR+/HER2− and HR+/HER2+ tumors, CTC ≥ 5 were significantly associated with lower survival times (HR = 1.96, 2.56 and 3.15 respectively). HR−/HER2+ subtype and IBC demonstrated no differences in survival according to baseline CTCs enumeration. Baseline enumeration have strong prognostic value except for HR−/HER2+ subtype treated with targeted therapy and IBC, suggesting that CTCs are more homogeneous in expression of HER2 before treatment, while patients with resistant disease may have cells with epithelial–mesenchymal transition that are undetectable by CellSearch® , suggesting the need to introduce new detection methods. http://dx.doi.org/10.1016/j.jbiotec.2014.07.333
Molecular cloning, over-expression, purification, and functional characterization of the meningococcal HrpA protein C-terminal domain Graziano Pizzolante ∗ , Roberta Romano, Carla Palumbo, Pietro Alifano Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy E-mail address: [email protected] (G. Pizzolante). The two-partner secretion (TPS) systems of Gram-negative bacteria consist of a large secreted exoprotein (TpsA) and a transporter protein (TpsB) located in the outer membrane. The functions identified for the TpsA proteins are numerous and contribute to bacterial fitness or virulence. Three different TPS systems in Neisseria meningitidis (meningococcus) are present. A previous study demonstrated that the meningococcal HrpB–HrpA TPS system was essential for intracellular growth/survival and escape from infected cells. Interestingly, the hrpA genes display significant C-terminal sequence variation. In this study, we highlighted by bioinformatics tools the presence of a putative hemolysin/hemagglutinin at the Cterminal domain of the HrpA protein. We cloned, over-expressed, purified and tested the protein activity on the human erythrocytes. The hemolytic activity assay showed that the C-terminal moiety induced erythrocytes lysis with MnCl2 . Instead, no hemolytic activity was recorded either in absence of ion supplement or in the presence of magnesium. Our data suggest that the HrpA protein Cterminal domain is a manganese-dependent lysin directly used by
Abstracts / Journal of Biotechnology 185S (2014) S37–S125
acid (C22:63, DHA), play multifunctional roles in human health and diseases. The traditional source of these fatty acids is fish. Since fish stocks are in global decline, new sources of omega-3 LC-PUFA have to be found. The aim of the present study was to find potential alternative sources of these fatty acids in the territory of The Republic of Kazakhstan. Twelve strains of filamentous fungi isolated from soil and water were screened for lipids and LCPUFA: Penicillium sp., Penicillium restrictum, Penicillium aculeatum, Penicillium anatolicum, Penicillium bilaiae, Penicillium commune, Penicillium raistrikii, Penicillium crustosum, Fusarium sp. (2 strains), Aspergillus sp. and Mucor circinelloides. These fungi were grown in Sabouraud medium for 10 days. Lipid contents of these strains ranged from 1.5% to 7.4% of dry weight. No LC-PUFA were found in P. commune, P. crustosum, Aspergillus sp., M. circinelloides, whereas other strains contained EPA ranging from 1.0% to 3.5% of the total fatty acids, P. restrictum accumulated the largest amount. The cultural conditions that can increase the production of EPA in P. restrictum (e.g. the addition of glucose, glycerin and zinc) are currently under investigation. http://dx.doi.org/10.1016/j.jbiotec.2014.07.332 Circulating tumor cells (CTCs) in association to breast cancer subtypes and inflammatory pattern Angela Toss 1,∗ , Jocelyn A. Sendecki 2 , Tiffany P. Avery 2 , James M. Reuben 3 , Massimo Cristofanilli 2 1 University of Modena and Reggio Emilia, Modena, Italy 2 Thomas Jefferson University, Philadelphia, PA, USA 3 The University of Texas MD Anderson Cancer Center, Huston, TX, USA
The phytochemical, antibacterial and antioxidant activity of wild plants growing in Kazakhstan against diabetic foot ulcer Tatyana Kustova 1,∗ , Tatyana Karpenyuk 1 , Alla Goncharova 1 , Samir Ross 2 1 Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan 2 School of Pharmacy, NCNPR, The University of Mississippi, University, USA
E-mail address: kus [email protected] (T. Kustova). Diabetic foot infection (DFI) is one of the most frequent and complex problems among patients suffering from diabetes. DFI is polymicrobial in nature. Moreover most of the bacteria have multidrug resistance. Therefore, much attention has been paid to find biological active substances from plants that help to solve this problem. Thus, the aim of this study was to evaluate phytochemical, antibacterial and antioxidant activity of crude extracts isolated from plants growing in Kazakhstan: Epilobium hirsutum L. (Onagraceae), Rumex confertus Wiild. (Polygonaceae), Vexibia alopecuroides (L.) Jakovl. (Fabaceae). The results of the present research clearly indicate that the antibacterial and antioxidant activities vary with the plant species. Among the different crude extract that were evaluated, the dichloromethane extract of roots of V. alopecuroides showed the strongest activity against Staphylococcus aureus (IC50 3.05 g/mL) and methicillin-resistant S. aureus (IC50 2.9 g/mL). Despite the fact that the extract of V. alopecuroides has not shown high antioxidant activity, it is still highly promising for the study because it showed high antimicrobial activity. Highest antioxidant activity (<85% inhibition of DPPH) was shown by E. hirsutum L. and R. confertus Wiild. Based on the research results plants were selected to produce drugs from their extracts. http://dx.doi.org/10.1016/j.jbiotec.2014.07.334
E-mail address: [email protected] (A. Toss). Circulating tumor cells (CTCs) are an independent prognostic factor for progression-free (PFS) and overall survival (OS) in metastatic breast cancer (MBC). The present analysis evaluates the prognostic value of CTCs in HER-2 positive disease receiving targeted therapies. This is a retrospective analysis of 516 patients with MBC from the MDACC database. The PFS and OS were evaluated in association to baseline CTCs (<5 vs. ≥5) within the different subtypes (HR+/HER2−, HR+/HER2+, HR−/HER2+, TNBC) and inflammatory breast cancer (IBC). Among 516 patients, 56% had HR+/HER2− tumor, 10% HR+/HER2+, 10% HR−/HER2+, and 24% were TNBC, including 25% with IBC. Overall, patients with CTCs ≥ 5 had a significantly lower median survival time (HR = 2.00). In TNBC, HR+/HER2− and HR+/HER2+ tumors, CTC ≥ 5 were significantly associated with lower survival times (HR = 1.96, 2.56 and 3.15 respectively). HR−/HER2+ subtype and IBC demonstrated no differences in survival according to baseline CTCs enumeration. Baseline enumeration have strong prognostic value except for HR−/HER2+ subtype treated with targeted therapy and IBC, suggesting that CTCs are more homogeneous in expression of HER2 before treatment, while patients with resistant disease may have cells with epithelial–mesenchymal transition that are undetectable by CellSearch® , suggesting the need to introduce new detection methods. http://dx.doi.org/10.1016/j.jbiotec.2014.07.333
Molecular cloning, over-expression, purification, and functional characterization of the meningococcal HrpA protein C-terminal domain Graziano Pizzolante ∗ , Roberta Romano, Carla Palumbo, Pietro Alifano Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Lecce, Italy E-mail address: [email protected] (G. Pizzolante). The two-partner secretion (TPS) systems of Gram-negative bacteria consist of a large secreted exoprotein (TpsA) and a transporter protein (TpsB) located in the outer membrane. The functions identified for the TpsA proteins are numerous and contribute to bacterial fitness or virulence. Three different TPS systems in Neisseria meningitidis (meningococcus) are present. A previous study demonstrated that the meningococcal HrpB–HrpA TPS system was essential for intracellular growth/survival and escape from infected cells. Interestingly, the hrpA genes display significant C-terminal sequence variation. In this study, we highlighted by bioinformatics tools the presence of a putative hemolysin/hemagglutinin at the Cterminal domain of the HrpA protein. We cloned, over-expressed, purified and tested the protein activity on the human erythrocytes. The hemolytic activity assay showed that the C-terminal moiety induced erythrocytes lysis with MnCl2 . Instead, no hemolytic activity was recorded either in absence of ion supplement or in the presence of magnesium. Our data suggest that the HrpA protein Cterminal domain is a manganese-dependent lysin directly used by