- Email: [email protected]
Comparative study of liver functional characteristics in combined pathologies by the method of electron paramagnetic resonance
JOURNAL OF CLINICAL AND EXPERIMENTAL HEPATOLOGY
Corresponding author: Shiran Shetty. E-mail: [email protected]
COMPARATIVE STUDY OF LIVER FUNCTIONAL CHARACTERISTICS IN COMBINED PATHOLOGIES BY THE METHOD OF ELECTRON PARAMAGNETIC RESONANCE Salome Kiparoidze, Eaterine Bakuradze, Irina Modebadze, Tamar Sanikidze Diana Dzidziguri Division of Morphology, Department of Biology, Faculty of Exact and Natural Sciences, Iv. Javakhishvili Tbilisi State University, Tbilisi Georgia
Background and Aims: It's established that in combined pathologies the ways and mechanisms of liver tissue restoration is determined by pathology and degree of damage. Up to date, in biological and medical research in order to study the changes of functional activity of organs or tissue different methods are widely implemented. By this point of view electron paramagnetic resonance (EPR) is especially useful, it gives detailed information about functional changes of intact and pathologically modified tissues and organs.The aim of the work was to determine functional characteristics of liver in combined pathologies by the EPR method. Methods: The investigations were carried out on 50 adult white rats (130-140 g). EPR signal changes from liver of intact and experimental animals were determined by the EPR method. Results and Discussion: In the cholestatic liver (the first four days) the nitrosyl complexes (HbNO) of mitochondrial heme-containing proteins were detected; free radicals EPR-signal intensity increased and its line widths reduced. This data indicate on the disorder of the hepatocytes mitochondrial electron transport chain on the NADH-ubiquinone-oxidoreductase segment. This is accompanied by development of hypoxia, decreasing of energogenesis (Mo5+ EPR signal was increased), enhanced formation of reactive oxygen species and disruption of membrane structure (Mn2+-containing complexes was increased). In the hormone imbalance conditions (bilateral adrenaleqtomy) mitochondrial electron transport chain's free radical signal
intensity was decreased which indicates impairment activity of the mitochondrial respiratory chain. After blocking the common bile duct in adrenaleqtomic rats, EPR- spectral changed in cholestatic liver are expressed more sharply. Results indicate that the degree of organ destruction in combined pathologies (bilateral adrenaleqtomy and cholestatisis) is stronger. Corresponding author: Salome Kiparoidze. E-mail: [email protected]
CLINICAL TRANSLATABLE EFFICIENCY OF A DENDRITIC CELL ENGINEERED VACCINE FOR GASTROINTESTINAL MALIGNANCIES Pradyumna Kumar Mishra Tata Memorial Centre, ACTREC, Navi Mumbai, India
The work attempts to overcome tumor associated immune tolerance using a surface modified solid lipid nanoparticles (SLNP) delivery system for dendritic cell (DC) immunotherapy. Different formulations of SLNP (SLNP-alone, cationic-SLNP and mannosylated-SLNP) were prepared using tumor cell lysates. Prepared nanoparticles were characterized and their ability to activate DCs for inducing a tumor cell specific response was assessed. SLNPs induced a strong phagocytic signal to DCs without any significant toxicity. Comparatively, mannosylated-SLNP evoked an optimum and effective cell mediated immune response with no significant toxicity. Surface modified SLNP may play a pivotal role in designing a clinically-translatable DC based immunotherapy for gastrointestinal malignancies. This novel approach might also facilitate the treatment of residual disease following standard therapy. Corresponding author: Pradyumna Kumar Mishra. E-mail: [email protected]
INTRAPERITONEAL PHOTODYNAMIC THERAPY FOR LOCALLY-ADVANCED CHOLANGIOCARCINOMA Kai-Wen Huang National Taiwan University, Taiwan
Background and Aim: Photodynamic therapy (PDT) is a procedure that combines a photosensitizer (PS), oxygen, and visible light to produce cytotoxic reactive oxygen species that kill cancers. This study was designed to demonstrate PDT a novel interventional treatment for locally advanced cholangiocarcinoma. Methods: We created an orthotropic animal model by injection of cholangiocarcinoma cells (HuCC-T1) over hilum area to assess tumor response after PDT. Photofrin was
Journal of Clinical and Experimental Hepatology | March 2013 | Vol. 3 | No. 1S | S118–S124
S121
Miscellaneous
liver enzymes (>3 times upper limit) along with jaundice, only 2 had liver enzymes more than 10 times the normal level., 4 patients had multiorgan failure. Conclusion: Hepatic dysfunction ranges from a mild elevation of liver enzymes to the range of acute hepatitis.The presence of hepatitis in patients with malaria indicates a more severe illness with a higher incidence of complications, further studies are required to elucidate the factors associated with malarial hepatopathy and to prevent the complications and mortality.
Corresponding author: Shiran Shetty. E-mail: [email protected]
COMPARATIVE STUDY OF LIVER FUNCTIONAL CHARACTERISTICS IN COMBINED PATHOLOGIES BY THE METHOD OF ELECTRON PARAMAGNETIC RESONANCE Salome Kiparoidze, Eaterine Bakuradze, Irina Modebadze, Tamar Sanikidze Diana Dzidziguri Division of Morphology, Department of Biology, Faculty of Exact and Natural Sciences, Iv. Javakhishvili Tbilisi State University, Tbilisi Georgia
Background and Aims: It's established that in combined pathologies the ways and mechanisms of liver tissue restoration is determined by pathology and degree of damage. Up to date, in biological and medical research in order to study the changes of functional activity of organs or tissue different methods are widely implemented. By this point of view electron paramagnetic resonance (EPR) is especially useful, it gives detailed information about functional changes of intact and pathologically modified tissues and organs.The aim of the work was to determine functional characteristics of liver in combined pathologies by the EPR method. Methods: The investigations were carried out on 50 adult white rats (130-140 g). EPR signal changes from liver of intact and experimental animals were determined by the EPR method. Results and Discussion: In the cholestatic liver (the first four days) the nitrosyl complexes (HbNO) of mitochondrial heme-containing proteins were detected; free radicals EPR-signal intensity increased and its line widths reduced. This data indicate on the disorder of the hepatocytes mitochondrial electron transport chain on the NADH-ubiquinone-oxidoreductase segment. This is accompanied by development of hypoxia, decreasing of energogenesis (Mo5+ EPR signal was increased), enhanced formation of reactive oxygen species and disruption of membrane structure (Mn2+-containing complexes was increased). In the hormone imbalance conditions (bilateral adrenaleqtomy) mitochondrial electron transport chain's free radical signal
intensity was decreased which indicates impairment activity of the mitochondrial respiratory chain. After blocking the common bile duct in adrenaleqtomic rats, EPR- spectral changed in cholestatic liver are expressed more sharply. Results indicate that the degree of organ destruction in combined pathologies (bilateral adrenaleqtomy and cholestatisis) is stronger. Corresponding author: Salome Kiparoidze. E-mail: [email protected]
CLINICAL TRANSLATABLE EFFICIENCY OF A DENDRITIC CELL ENGINEERED VACCINE FOR GASTROINTESTINAL MALIGNANCIES Pradyumna Kumar Mishra Tata Memorial Centre, ACTREC, Navi Mumbai, India
The work attempts to overcome tumor associated immune tolerance using a surface modified solid lipid nanoparticles (SLNP) delivery system for dendritic cell (DC) immunotherapy. Different formulations of SLNP (SLNP-alone, cationic-SLNP and mannosylated-SLNP) were prepared using tumor cell lysates. Prepared nanoparticles were characterized and their ability to activate DCs for inducing a tumor cell specific response was assessed. SLNPs induced a strong phagocytic signal to DCs without any significant toxicity. Comparatively, mannosylated-SLNP evoked an optimum and effective cell mediated immune response with no significant toxicity. Surface modified SLNP may play a pivotal role in designing a clinically-translatable DC based immunotherapy for gastrointestinal malignancies. This novel approach might also facilitate the treatment of residual disease following standard therapy. Corresponding author: Pradyumna Kumar Mishra. E-mail: [email protected]
INTRAPERITONEAL PHOTODYNAMIC THERAPY FOR LOCALLY-ADVANCED CHOLANGIOCARCINOMA Kai-Wen Huang National Taiwan University, Taiwan
Background and Aim: Photodynamic therapy (PDT) is a procedure that combines a photosensitizer (PS), oxygen, and visible light to produce cytotoxic reactive oxygen species that kill cancers. This study was designed to demonstrate PDT a novel interventional treatment for locally advanced cholangiocarcinoma. Methods: We created an orthotropic animal model by injection of cholangiocarcinoma cells (HuCC-T1) over hilum area to assess tumor response after PDT. Photofrin was
Journal of Clinical and Experimental Hepatology | March 2013 | Vol. 3 | No. 1S | S118–S124
S121
Miscellaneous
liver enzymes (>3 times upper limit) along with jaundice, only 2 had liver enzymes more than 10 times the normal level., 4 patients had multiorgan failure. Conclusion: Hepatic dysfunction ranges from a mild elevation of liver enzymes to the range of acute hepatitis.The presence of hepatitis in patients with malaria indicates a more severe illness with a higher incidence of complications, further studies are required to elucidate the factors associated with malarial hepatopathy and to prevent the complications and mortality.