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Complement and the heart: C5b-9 directly modifies calcium content and contractility of isolated cardiac myocytes
J Mol p-05-11
Cell
Cardiol
24 (Supplement
I) (1992)
RENAL CA*++MG*+AlPASE IS LINKED TO ATRIAL NAlRlUREllC PEF’TIDE-RECEPTCR SYSTEM: A PARADIGM IN CONGESTWE HEART FAILURE. Adme& Sahai and PaHab K Ganguly. Dtv. Cardbvasc. Scl. St. Sonlface Rw. Ctr. and Dept. of Anatomy, Univ. cf Manitoba, Winntpag,Canada R2H 2At3 TheCa**+Md*ATPasewhlchaervesasaCaZ*w~Inthekidnevbesdeteralmembrene~LMIisessanrislto Since atrial r&i&& peptlde the maintenan& of an lntracelluiar Ca*’ xmcent&on bptirnal for k&y functi&. and Is involved in the dlracl stimulaUon of Ca” wtma In fANP) Is knmm to Dartkbate in the Ca” homeosta& The ATFase a&&&s l&G BLh4, we fl& inve&gated C Ca” +M$ATPase is linked to ANP-receptor system. measured by the release d =P from (r-*]ATP in the medium. lncubaticn of ELM v by the differential centtifugatbn procedure in rat kidney cortex In the presence of ANP increased Ca ‘+Md’ATPase in a doseHowever, adoration of guanylate cyclase, a well-known receptor for ANP. by GTP and Mg” dependent manner. inhibited this AT&se activity; such a response was mversed by antlbcdy raked against both AN; and guanylate c$$E& Non hydrdysable GTP such as 0TP-y-S as well as cydk GMP had no effect on the Ca pump system. est that ANP mediates its cellular effects in part by changes In Ca” hcmewtasls In kidney cortex and Ca’++MT+ ATPase Is linked to ANP receptors being reclprocaWy modulated by guanylate cydase system. In the second phase, Ca*+ + Mg*+ ATPase and guanyiate cyclase were tested In hypertensfvedlabetk rats (D + H) which develop congestive heart failure (CHF) at 10 weeks fdlowhrg streptozotocin (65 mg/kg) Injection and alxbmlnal aortk constrlctl~. While the guanylate cydase activity was decreased very rapklly in this experlmentai group, the senskMty of Ca” pump to ANP was Increased at ea$ stage 43 weeks) &xi~de&ased at.!ate stage (10 weeks) of CHF. We conclude that a defect In couplkrg betwean CE ’ pump and ANP-receotor svstem as O~WIWLI In the 0 +H group may ~oMbute to the de&pm&t of nephropattiy and CHF.(Suppo&d by &tad&n Diabetes Association)
P45~2HIGR ATRIAL NATRIURETIC PEPTIDES(ANP) LEVELS IN THE PERICARDIAL FLUID IN PATIANTS WITH CARDIAC DISEASE Jun Amano, Akio Suzuki, Makoto Sunamori, Toshizumi Shirai, Hitoshi Fujiwara, Masayoshi Shichiri, Fumiaki Marumo. Tokyo Medical and Dental University, Tokyo 113, and Hokushin General Hospital, Nagano 387, JAPAN The present study was aimed to measure the ANP levels in the pericardial fluid in patients with aquired heart disease. We examined 20 patients who underwent cardiac surgery and 19 healthy volunteers. ANP leve1(319.2*49.6pg/ml) was significantly higher than the corresponding plasma level (125*29.5pg/ml), and this plasma level was hiaher than that of the healthv volunteers No difference was observed betwee: pericardial <46.5*4.31pg/ml). and plasma c-GMP levels. Pericardial and plasma ANP levels showed significant positive correlation, however no correlations were observed between pericardial ANP levels and hemodynamic parameters. In conclusion, high ANP levels in the pericardial fluid were demonstrated, and there were no evidences that pericardial release of ANP was specifically coupled to hemodynamics.
p-&j3
z;;oPEylI$ Central
AND 9 Drug
R sssarch
CALCIUM
PARADOX
Instltute,
Luoknow,
IN THE
RAT HEART
A 31P-NMR
STUDY.
India
Isolated rat hearts were perfused rstrogradely (Langandorf’f) at 37’C ure of 75 mm Hg. ATP and phosphocrsatine (PCr) were monitored prT? P-NMR. by APtret- 30 min. control psrpusion the hsarts were perfused Ca2’-free buffer and the reperfussd 15 min. with normal buffer. Trifluoperzene 10% wae added in the buffer during calcium free and calcium repeusion group. A second group was exposed to TFP only during calcium tree repert’usion. The ATP and PCr peeks were close to normal levels. The prutscted by trifluoperazine Pran the ePl%cts aP ‘Calcium Paradox’. 5.205
at a constant continuously 10 min. with (TFP) in one and calcium
hear-te
were
Cell
Cardiol
24 (Supplement
I) (1992)
RENAL CA*++MG*+AlPASE IS LINKED TO ATRIAL NAlRlUREllC PEF’TIDE-RECEPTCR SYSTEM: A PARADIGM IN CONGESTWE HEART FAILURE. Adme& Sahai and PaHab K Ganguly. Dtv. Cardbvasc. Scl. St. Sonlface Rw. Ctr. and Dept. of Anatomy, Univ. cf Manitoba, Winntpag,Canada R2H 2At3 TheCa**+Md*ATPasewhlchaervesasaCaZ*w~Inthekidnevbesdeteralmembrene~LMIisessanrislto Since atrial r&i&& peptlde the maintenan& of an lntracelluiar Ca*’ xmcent&on bptirnal for k&y functi&. and Is involved in the dlracl stimulaUon of Ca” wtma In fANP) Is knmm to Dartkbate in the Ca” homeosta& The ATFase a&&&s l&G BLh4, we fl& inve&gated C Ca” +M$ATPase is linked to ANP-receptor system. measured by the release d =P from (r-*]ATP in the medium. lncubaticn of ELM v by the differential centtifugatbn procedure in rat kidney cortex In the presence of ANP increased Ca ‘+Md’ATPase in a doseHowever, adoration of guanylate cyclase, a well-known receptor for ANP. by GTP and Mg” dependent manner. inhibited this AT&se activity; such a response was mversed by antlbcdy raked against both AN; and guanylate c$$E& Non hydrdysable GTP such as 0TP-y-S as well as cydk GMP had no effect on the Ca pump system. est that ANP mediates its cellular effects in part by changes In Ca” hcmewtasls In kidney cortex and Ca’++MT+ ATPase Is linked to ANP receptors being reclprocaWy modulated by guanylate cydase system. In the second phase, Ca*+ + Mg*+ ATPase and guanyiate cyclase were tested In hypertensfvedlabetk rats (D + H) which develop congestive heart failure (CHF) at 10 weeks fdlowhrg streptozotocin (65 mg/kg) Injection and alxbmlnal aortk constrlctl~. While the guanylate cydase activity was decreased very rapklly in this experlmentai group, the senskMty of Ca” pump to ANP was Increased at ea$ stage 43 weeks) &xi~de&ased at.!ate stage (10 weeks) of CHF. We conclude that a defect In couplkrg betwean CE ’ pump and ANP-receotor svstem as O~WIWLI In the 0 +H group may ~oMbute to the de&pm&t of nephropattiy and CHF.(Suppo&d by &tad&n Diabetes Association)
P45~2HIGR ATRIAL NATRIURETIC PEPTIDES(ANP) LEVELS IN THE PERICARDIAL FLUID IN PATIANTS WITH CARDIAC DISEASE Jun Amano, Akio Suzuki, Makoto Sunamori, Toshizumi Shirai, Hitoshi Fujiwara, Masayoshi Shichiri, Fumiaki Marumo. Tokyo Medical and Dental University, Tokyo 113, and Hokushin General Hospital, Nagano 387, JAPAN The present study was aimed to measure the ANP levels in the pericardial fluid in patients with aquired heart disease. We examined 20 patients who underwent cardiac surgery and 19 healthy volunteers. ANP leve1(319.2*49.6pg/ml) was significantly higher than the corresponding plasma level (125*29.5pg/ml), and this plasma level was hiaher than that of the healthv volunteers No difference was observed betwee: pericardial <46.5*4.31pg/ml). and plasma c-GMP levels. Pericardial and plasma ANP levels showed significant positive correlation, however no correlations were observed between pericardial ANP levels and hemodynamic parameters. In conclusion, high ANP levels in the pericardial fluid were demonstrated, and there were no evidences that pericardial release of ANP was specifically coupled to hemodynamics.
p-&j3
z;;oPEylI$ Central
AND 9 Drug
R sssarch
CALCIUM
PARADOX
Instltute,
Luoknow,
IN THE
RAT HEART
A 31P-NMR
STUDY.
India
Isolated rat hearts were perfused rstrogradely (Langandorf’f) at 37’C ure of 75 mm Hg. ATP and phosphocrsatine (PCr) were monitored prT? P-NMR. by APtret- 30 min. control psrpusion the hsarts were perfused Ca2’-free buffer and the reperfussd 15 min. with normal buffer. Trifluoperzene 10% wae added in the buffer during calcium free and calcium repeusion group. A second group was exposed to TFP only during calcium tree repert’usion. The ATP and PCr peeks were close to normal levels. The prutscted by trifluoperazine Pran the ePl%cts aP ‘Calcium Paradox’. 5.205
at a constant continuously 10 min. with (TFP) in one and calcium
hear-te
were