Detection of Cellular Retinol-Binding Protein messenger RNA in the somatic cells of the rat seminiferous tubules

Vol.

154,

August

No. 15,

3,

1988

BIOCHEMICAL

IETECTION

CELLULAR SOMAT

RETINOL-BINDING

IC

CELLS

OF

lstituto

di

THE

lstologia

ed

lstituto

di

SC i enze Chi

Received

cDNA

as

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June

28,

clone to

these

specific

mRNA.

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effective

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mammals

Sertoli

because

cells

are

(FSH)

Napol

i,

di

di

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Medicina

e

Naples

Protein gene

in ccl

gene

and two

when

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this

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paper

the

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testosterone. times

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FSH

was

somatic

Is). in

is

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the

involved

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maturation

(1,2).

from

the many

lular

Moreover, concentration i

ccl

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cells the

the

such their and

$1.50

0 1988 by Academic Press, Inc. of reproduction in any form reserved.

1174

of

action their

fol

this

but

process.

Sertoli hormone

these

cells

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Istologia

only

epithelium

act

necessary

di

are

not

I icle-stimulating on

which

tubules

spermatogenesis

of as

where in

the

seminioferous

microenvironment

Gal dieri, Istituto ROMA 00161, Italy

tubules

compartment

regulation

hormones

the

seminiferous

adluminal

of

hormonal

exert

the

somatic

metabolism

create

Scarpa

the

These

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to: A.

in

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place

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the

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approximately

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TUBULES

and

Universith

a,

for

study

of CRBP steady-state 0 1988 Academic press,

SEMINIFEROUS

1174-1181

1988

tubules

demonstrate

RAT

Biochimiche,

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coding

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seminiferous

In

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MESSENGER

Embriologia

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BIOPHYSICAL

Pages

OF

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1988

directly, iv i ty

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Vol.

154,

No.

Retinol

is

(3,4,5)

and

for

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the

cellular

the

cells

3,

1988


essential in

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Vol.

154,

No.

3, 1988

monolayer

overlaid

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Vol.

154,

No.

Fig.1.

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Dot

Five

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1988

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Vol.

154,

No.

Cyclic ex

a

BIOCHEMICAL

1988

AMP

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on.

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at

3,

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Vol.

154,

effect,

No.

3,

in

line

secretory

proteins

In

to

order

present

in

blot

with

compare cell

cells

are

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i

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days

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monolayer.

at RNA

and cells

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that

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in

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these

two

influence

could low

and

(fig.4,

protein

time

Sertoli

blot

mRNA mRNA,

plated

than materials

two

culture

tubules

RNA

the

that

cells.

Sertoli

CRBP

of

were

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CRBP,

(see

approximately

if

of

seminiferous

prepare

the

abundance

evaluate

gene, get

ative

with

peritubular

confluency.

the

compared

higher

to

synthesize

this

the

isolated

reached

of

rel

from

concentrations were

levels

express

(17).

at

they

level

of

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u nt i I

Sertoli

high

component

animals

the

of ten

mRNA

COMMUNICATIONS

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cells

these

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expression

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contain

detected

same days

cells

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RESEARCH

the

RNAs

somatic

tubular

Comparing

peritubular

for

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to on

been peri

CRBP

known

other

has

The

cultured

BIOPHYSICAL

in

of

performed the

from

level

types

was

methods)

reported

the

other

analysis

Is

data

AND

(9,10,12).

These

ccl

BIOCHEMICAL

1988

density

extracted

the

and from

these

cultured cells

-28s

N-18

1

Fig The

.4.

CRBP RNA5

experimental

mRNA were

levels

in

extracted conditions

are

2

Sertoli from the

s

and

peritubular

Sertol

i

same

as

1179

(1) those

and

cells. peritubular described

(2) in

fig.2

cells. legend.

The

Vol.

154,

was cul

No.

A

in

not

conclusion

som

at i c

of

the

and

intensity

did

not

modify

BIOPHYSICAL

RESEARCH

compared

to

was

obtained,

the

level

COMMUNICATIONS

that

from

short-time

indicating of

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that mRNA

the

synthesis

shown).

from

the

the

RNA is

shown

the

mirror by

spermatogenetic

coding

protein

some

we

tubules,

gene

the

regulated

here

seminiferous

express

steady-state gene

experiments

of

actively

the

same

culture

components

Is,

AND

blot

Northern

band

time

In

in

BIOCHEMICAL

by

tures.

(data

the

1988

analyzed

longer

ccl

3,

of

the

establish

Sertol for

levels

can i

CRBP.

in

hormones

and

peri

The

both

that

cell

tubul

levels

to

ar

of

types.

known

the

the

Moreover,

affect

other

genes

process.

ACKNOWLEDGMENT This

work

was

supported

by

a

grant

from

the

MPI

(40%).

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