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Effect of pH and aeration on trehalose and biomass production by Propionibacterium freudenreichii ssp. shermanii 1
Abstracts / New Biotechnology 33S (2016) S1–S213
Reference [1] Wang TW, Sun JS, Wu HC, Tsuang YH, et al. The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice. Biomaterials 2006;27:5689–97.
http://dx.doi.org/10.1016/j.nbt.2016.06.1379
P32-6 Development of a method for the construction of artificial genes coding for bioactive peptide-based biopolymers 1,∗ , Piotr Skowron 1 , Olga ˙ Agnieszka Zylicz-Stachula 2 , Małgorzata Skowron 1 , Joanna ˙ Zołnierkiewicz 2 , Daria Krefft 3 , Dawid Nidzworski 1 , Kasjan ˙ ˛ Jezewska Frackowiak ˙ 2 , Aneta Szemiako 2 , Natalia Maciejewska 1 , Agnieszka Ozóg 3 , Łukasz Janus 3 , Alicja Wiercinska-Drapało 3, ´ ´ Szymanska 1 ˛ Grzegorz Wegrzyn
S191
The aim of this study was to examine the effect of pH and aeration on trehalose and biomass production by Propionibacterium freudenreichii ssp. shermanii 1. To evaluate the effect of pH on the trehalose and biomass production, the cultures were carried out at four different pH values (5.0, 6.0, 7.0, 8.0). To verify the effect of aeration on trehalose and biomass production, the cultures were carried out under aerobic conditions (30% air saturation in a batch reactor). The highest amount of trehalose – more than 10% of dry biomass, was achieved in cultures with the lowest pH value. Whereas the maximal biomass was reached under aerobic conditions and it was two times higher than that obtained in cultures with no aeration. The results indicate that the aeration and pH of the culture have significant influence on bacterial growth and trehalose accumulation. http://dx.doi.org/10.1016/j.nbt.2016.06.1381
University of Gdansk, Poland Faculty of Chemistry, Poland 3 Bioventures Institute Ltd, Poland
P32-8
An improved version of a method for peptide-based biopolymers synthesis was further developed. The method recruits Type IIS restriction endonuclease SapI, which recognizes 5 -GCTCTTC-3 DNA sequence and cleaves at a distance of 1/4 nucleotides. A series of specially designed vectors was constructed, enabling the efficient amplification of any DNA sequence. The vectors were designed to create artificial long Open reading Frames, coding for biopolymers with potential biological activity, which do not occur in nature. The usefulness of the method has been tested for eight different constructs. The method could be useful for a number of scientific and industrial applications, such as development of recombinant vaccines or regenerative medicine research. The technology is protected by an international patent application. Acknowledgments: Project was supported by National Center for Research and Development, Warsaw, Poland, grant no STRATEGMED1/235077/9/NCBR/2014 and POIG.01.04.00-22140/12; Jagiellonian Center for Innovation, Krakow, Poland; SATUS VC, Warsaw, Poland and BioVentures Institute Ltd, Poznan, Poland.
Tsutomu Tanaka ∗ , Hiroki Nishikawa, Naoki Ikeda, Akihiko Kondo
1 2
Putrescine production from cellobiose by cell surface- and metabolically-engineered E. coli
Kobe University, Japan Putrescine (1,4-diaminobutane) has many industrial applications as a component of polymers, agrochemicals, surfactants, pharmaceuticals, and other additives. Here, we demonstrated direct putrescine production using beta-glucosidase displaying and metabolically engineered E. coli strain. Ornithine decarboxylase (SpeC) derived from E. coli and BGL from Thermobifida fusca YX fused to the anchor protein Blc from E. coli were co-expressed using E. coli as a host. To improve putrescine production, putrescine degradation pathways were deleted, and feedback inhibition by arginine was deregulated by argI disruption. The engineered strain produced 1.37 g/L of putrescine from 10 g/L of glucose with a yield of 1.37 gputrescine/g-glucose. Finally, 0.41 g/L of putrescine production was achieved from 10 g/L of cellobiose. http://dx.doi.org/10.1016/j.nbt.2016.06.1382
http://dx.doi.org/10.1016/j.nbt.2016.06.1380 P32-9 P32-7 Effect of pH and aeration on trehalose and biomass production by Propionibacterium freudenreichii ssp. shermanii 1 ˛ Joanna Pawlicka-Kaczorowska ∗ , Ilona Madrawska, Katarzyna Czaczyk Poznan University of Life Sciences, Poland Propionic acid bacteria are Gram-positive, facultative anaerobic, pleomorphic rods – belonging to the genus Propionibacterium. Traditionally, they are used as starters in the manufacture of Swisstype cheeses. A growing number of studies are concerned with their potential as probiotics. Dairy propionibacteria are able to improve the health of animals and humans by modulating gut microbiota, regulating immune system or causing colorectal carcinoma cells apoptosis. They can also produce functional biomolecules e.g. B group vitamins, propionic acid or trehalose. The latter metabolite, trehalose is sugar with nutraceutical value which possesses unique chemical and/or physical properties.
A novel systems dynamics model for simulation of yeast batch, fed-batch and continuous cultures Elisabetta de Alteriis 1,∗ , Palma Parascandola 2 , Stefano Mazzoleni 3 , Francesco Giannino 3 , Fabrizio Cartenì 1 1
University of Naples, Italy University of Salerno, Italy 3 University of Naples “Federico II”, Italy 2
Modelling of microbial cell cultures is essential for design, optimization and control of processes of biotechnological interest. Models can vary from the simple “black box” descriptions to more complex “cybernetic” approaches, but they usually lack flexibility in representing the microbial population dynamics including feedbacks from environment. On the contrary, the innovative model recently proposed [1] and based on the principle of Systems Dynamics, highlights how the decline and arrest of cell proliferation depends on the accumulation of self-produced inhibitory compounds in the medium. The model (developed in SIMILE and MATLAB R2012b) focused on the
Reference [1] Wang TW, Sun JS, Wu HC, Tsuang YH, et al. The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice. Biomaterials 2006;27:5689–97.
http://dx.doi.org/10.1016/j.nbt.2016.06.1379
P32-6 Development of a method for the construction of artificial genes coding for bioactive peptide-based biopolymers 1,∗ , Piotr Skowron 1 , Olga ˙ Agnieszka Zylicz-Stachula 2 , Małgorzata Skowron 1 , Joanna ˙ Zołnierkiewicz 2 , Daria Krefft 3 , Dawid Nidzworski 1 , Kasjan ˙ ˛ Jezewska Frackowiak ˙ 2 , Aneta Szemiako 2 , Natalia Maciejewska 1 , Agnieszka Ozóg 3 , Łukasz Janus 3 , Alicja Wiercinska-Drapało 3, ´ ´ Szymanska 1 ˛ Grzegorz Wegrzyn
S191
The aim of this study was to examine the effect of pH and aeration on trehalose and biomass production by Propionibacterium freudenreichii ssp. shermanii 1. To evaluate the effect of pH on the trehalose and biomass production, the cultures were carried out at four different pH values (5.0, 6.0, 7.0, 8.0). To verify the effect of aeration on trehalose and biomass production, the cultures were carried out under aerobic conditions (30% air saturation in a batch reactor). The highest amount of trehalose – more than 10% of dry biomass, was achieved in cultures with the lowest pH value. Whereas the maximal biomass was reached under aerobic conditions and it was two times higher than that obtained in cultures with no aeration. The results indicate that the aeration and pH of the culture have significant influence on bacterial growth and trehalose accumulation. http://dx.doi.org/10.1016/j.nbt.2016.06.1381
University of Gdansk, Poland Faculty of Chemistry, Poland 3 Bioventures Institute Ltd, Poland
P32-8
An improved version of a method for peptide-based biopolymers synthesis was further developed. The method recruits Type IIS restriction endonuclease SapI, which recognizes 5 -GCTCTTC-3 DNA sequence and cleaves at a distance of 1/4 nucleotides. A series of specially designed vectors was constructed, enabling the efficient amplification of any DNA sequence. The vectors were designed to create artificial long Open reading Frames, coding for biopolymers with potential biological activity, which do not occur in nature. The usefulness of the method has been tested for eight different constructs. The method could be useful for a number of scientific and industrial applications, such as development of recombinant vaccines or regenerative medicine research. The technology is protected by an international patent application. Acknowledgments: Project was supported by National Center for Research and Development, Warsaw, Poland, grant no STRATEGMED1/235077/9/NCBR/2014 and POIG.01.04.00-22140/12; Jagiellonian Center for Innovation, Krakow, Poland; SATUS VC, Warsaw, Poland and BioVentures Institute Ltd, Poznan, Poland.
Tsutomu Tanaka ∗ , Hiroki Nishikawa, Naoki Ikeda, Akihiko Kondo
1 2
Putrescine production from cellobiose by cell surface- and metabolically-engineered E. coli
Kobe University, Japan Putrescine (1,4-diaminobutane) has many industrial applications as a component of polymers, agrochemicals, surfactants, pharmaceuticals, and other additives. Here, we demonstrated direct putrescine production using beta-glucosidase displaying and metabolically engineered E. coli strain. Ornithine decarboxylase (SpeC) derived from E. coli and BGL from Thermobifida fusca YX fused to the anchor protein Blc from E. coli were co-expressed using E. coli as a host. To improve putrescine production, putrescine degradation pathways were deleted, and feedback inhibition by arginine was deregulated by argI disruption. The engineered strain produced 1.37 g/L of putrescine from 10 g/L of glucose with a yield of 1.37 gputrescine/g-glucose. Finally, 0.41 g/L of putrescine production was achieved from 10 g/L of cellobiose. http://dx.doi.org/10.1016/j.nbt.2016.06.1382
http://dx.doi.org/10.1016/j.nbt.2016.06.1380 P32-9 P32-7 Effect of pH and aeration on trehalose and biomass production by Propionibacterium freudenreichii ssp. shermanii 1 ˛ Joanna Pawlicka-Kaczorowska ∗ , Ilona Madrawska, Katarzyna Czaczyk Poznan University of Life Sciences, Poland Propionic acid bacteria are Gram-positive, facultative anaerobic, pleomorphic rods – belonging to the genus Propionibacterium. Traditionally, they are used as starters in the manufacture of Swisstype cheeses. A growing number of studies are concerned with their potential as probiotics. Dairy propionibacteria are able to improve the health of animals and humans by modulating gut microbiota, regulating immune system or causing colorectal carcinoma cells apoptosis. They can also produce functional biomolecules e.g. B group vitamins, propionic acid or trehalose. The latter metabolite, trehalose is sugar with nutraceutical value which possesses unique chemical and/or physical properties.
A novel systems dynamics model for simulation of yeast batch, fed-batch and continuous cultures Elisabetta de Alteriis 1,∗ , Palma Parascandola 2 , Stefano Mazzoleni 3 , Francesco Giannino 3 , Fabrizio Cartenì 1 1
University of Naples, Italy University of Salerno, Italy 3 University of Naples “Federico II”, Italy 2
Modelling of microbial cell cultures is essential for design, optimization and control of processes of biotechnological interest. Models can vary from the simple “black box” descriptions to more complex “cybernetic” approaches, but they usually lack flexibility in representing the microbial population dynamics including feedbacks from environment. On the contrary, the innovative model recently proposed [1] and based on the principle of Systems Dynamics, highlights how the decline and arrest of cell proliferation depends on the accumulation of self-produced inhibitory compounds in the medium. The model (developed in SIMILE and MATLAB R2012b) focused on the