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Effects of autologous human serum on culturing primary osteoblast cells
212 Autologous demineralised tooth matrix used as a biomaterial for alveolar ridge preservation W. Ouyyamwong ∗ , S. Suttapreyasri, N. Leepong, B. Samruajbenjakun Prince of Songkla University, Songkhla, Thailand Background: Various bone substitutes are employed for ridge preservation. Tooth has been attracting attentions as a material for alveolar bone regeneration. Several studies showed that tooth possessed osteoconductive and osteoinductive properties similar to bone. Objective: To determine the efficacy of autologous demineralised tooth matrix (auto-DTM) in the preservation of alveolar ridge shape after tooth extraction. Methods: 40 symmetrical premolar extraction sockets using split-mouth design were randomly filled with auto-DTM and covered with platelet-rich fibrin (PRF) [DTM group] or PRF alone (control group). The evaluation of alveolar ridge dimensional changes, marginal bone resorption and bone healing density were performed in two-dimensional computer-based analysis and periapical radiographs (immediate, two weeks, four weeks, six weeks and eight weeks). Findings: Auto-DTM was well tolerated in all sites without incidences of postoperative complication. The dimensions of the ridges were significantly better preserved in DTM group than in control group. The radiographic resorption of marginal bone on the mesial side, distal side, as well as height of the sockets in the DTM group were not significantly different from those of the control group. Conclusions: Auto-DTM can be a useful alternative graft material for ridge preservation. Extraction socket grafted with auto-DTM and covered with PRF can reduce buccal bone collapse and facilitate bone-healing density as shown clinically and radiographically for short term ridge preservation. http://dx.doi.org/10.1016/j.ijom.2017.02.719 Effects of autologous human serum on culturing primary osteoblast cells M. Phopetch ∗ , N. Thuaksuban, T. Nuntanaranont, N. Leepong, S. Vongvatcharanon Prince of Songkhla University, Songkhla, Thailand Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Songkhla, Thailand Background: Using engineered bone tissue for clinical trials encounters contamination of xenogenic components of fetal bovine serum (FBS) used for cell culture procedure. This study investigated the feasibility of using autologous human serum (AHS) for human cell culture instead of FBS. Objectives: To comparatively evaluate growth and differentiation of human osteoblast cells cultured in mediums supplemented with AHS, FBS and serum free mediums (SFM). Methods: Primary osteoblast cells harvested during orthognathic surgeries from six patients (two males, four females; age 36.25 ± 16.04 years) were cultured in three different groups of mediums including AHS group: Alpha-MEM mediums with 10% AHS from 50 mL venous blood of each patient; FBS group: the medium with 10% FBS; and SFM group: STEMPRO, United
States of America. Proliferation and differentiation of the cells among the groups were comparatively assessed over 30 days using ELISA. Findings: Growth of the cells in AHS group was comparable to FBS group, whereas the cells in SFM group had significantly lower proliferation (P 0.05), whereas those in SFM group were stable over the observation period. Conclusion: The AHS used as the supplement in the culture medium was comparable to the FBS in terms of supporting growth and differentiation of the autogenous primary osteoblast cells. http://dx.doi.org/10.1016/j.ijom.2017.02.720 The ultrastructural features of re-epithelialisation process of surgical wounds on oral mucosa and facial skin C.R. Rahimov ∗ , E. Qasimov, T.R. Quliyev, I.M. Farzaliyev Azerbaijan Medical University, Baku, Azerbaijan Background: The question concerning migration process of keratinocytes within re-epithelialisation of surgical wounds is still debating. The aim of the present study was to investigate the dynamic relationship between keratinocytes itself and with connective tissue elements on ultrastructural level during reepithelialisation process of surgical wound on facial skin and oral mucosa. Methods: The model of full-thickness surgical wounds on skin and oral mucosa were created in domestic pigs under the general anaesthesia. The tissue taken from the wound was kept in fixating solution containing 2.5% paraformaldehyde, 2.0% glutaraldehyde and 0.1% picric acid in 0.1 M phosphate-buffered saline for at least 4 h and then processed using standard electron microscopy. Ultrathin sections were examined by using transmission electron microscopy. Results: The “epithelial regeneration lingua” composed from different number of keratinocytes is forming on the wound edges with a further tendency to migration toward the bottom of the wound. In the area of beginning of epithelial lingua as well on the part directed toward bottom of the wound the main bulk of keratinocytes are losing their basoapical polarisation and because of their fattening are localising parallel to the edges of surround connective tissue elements. It is character by the forming of cytoplasmatic bridges on the background of existing desmosomes inbetween. Conclusions: This novel study demonstrates that reepithelialisation of surgical wound in oral mucosa and skin is associating with migration of keratinocytes not as single cell migration but as cell layer. These findings could be applicable in surgical wounds management. http://dx.doi.org/10.1016/j.ijom.2017.02.721
States of America. Proliferation and differentiation of the cells among the groups were comparatively assessed over 30 days using ELISA. Findings: Growth of the cells in AHS group was comparable to FBS group, whereas the cells in SFM group had significantly lower proliferation (P 0.05), whereas those in SFM group were stable over the observation period. Conclusion: The AHS used as the supplement in the culture medium was comparable to the FBS in terms of supporting growth and differentiation of the autogenous primary osteoblast cells. http://dx.doi.org/10.1016/j.ijom.2017.02.720 The ultrastructural features of re-epithelialisation process of surgical wounds on oral mucosa and facial skin C.R. Rahimov ∗ , E. Qasimov, T.R. Quliyev, I.M. Farzaliyev Azerbaijan Medical University, Baku, Azerbaijan Background: The question concerning migration process of keratinocytes within re-epithelialisation of surgical wounds is still debating. The aim of the present study was to investigate the dynamic relationship between keratinocytes itself and with connective tissue elements on ultrastructural level during reepithelialisation process of surgical wound on facial skin and oral mucosa. Methods: The model of full-thickness surgical wounds on skin and oral mucosa were created in domestic pigs under the general anaesthesia. The tissue taken from the wound was kept in fixating solution containing 2.5% paraformaldehyde, 2.0% glutaraldehyde and 0.1% picric acid in 0.1 M phosphate-buffered saline for at least 4 h and then processed using standard electron microscopy. Ultrathin sections were examined by using transmission electron microscopy. Results: The “epithelial regeneration lingua” composed from different number of keratinocytes is forming on the wound edges with a further tendency to migration toward the bottom of the wound. In the area of beginning of epithelial lingua as well on the part directed toward bottom of the wound the main bulk of keratinocytes are losing their basoapical polarisation and because of their fattening are localising parallel to the edges of surround connective tissue elements. It is character by the forming of cytoplasmatic bridges on the background of existing desmosomes inbetween. Conclusions: This novel study demonstrates that reepithelialisation of surgical wound in oral mucosa and skin is associating with migration of keratinocytes not as single cell migration but as cell layer. These findings could be applicable in surgical wounds management. http://dx.doi.org/10.1016/j.ijom.2017.02.721