Effects of gemfibrozil administration on very low density lipoprotein receptor mRNA levels in rabbits N
M N
H S
J
J T
H
M
T
M
T
a S N
M K a
Y
i
“Departmentof Medicineand ClinicalScience, Kyoto UniversityGraduateSchool of Medicine, 54 Shogoin-Kawahara -cho, Sakyo-kte, Kyoto 606-01,Japan bDepartmentof Internal Medicine, Osaka Red CrossHospital, Osaka, Japan ‘Gene Research Center, Tohoku University,Sendai,Japan R
7N
1
r
2 F
1
a
1 M
1
e
A
o
b T e
r p
f
1 p
r m 3 r w V o a g m l m c a m
t d
r
o v l a f r
g
d a
V i r w g t r i m h e a L w a i i p t t i o t
m
l
Keywords: V
r
G
(
l d
a
a w e
lr
a l
rl el
e a i g n m ec I t l V r l ( a ld i a t T e l c t b
e g
e d
w t a
i
a oa
a i i
b N b e m w ie w o . i te h m w n d e a c A s e o V r i rp o t s a u
R
e
t
e
e a
m r
T Ve 6 a at t o ri s e eb x i ( m nm a t in
e
1 I *C s
r +
a I
B o M
D
S
O
5
J
E B + 8 6 8
T
o 6
R F f
[ l
6
0
PZZS0021-
5
Q 1 1
E
0
S
I
L
A
r
Vn
a
n l d e i am (
i
rn
l
o t m r s 8
2 r
1 0
(
o
l
l
u r e
d u [
i
m p
s 7
i -
c
r
222
N. Ma(suoka et a[./ Alherosc[erosis126 (1996)221-226
T I o V r a a E l ( ~ V j a i d l p ( [ R a p ( w w d d p o l d l r p i ( a b t V r m [ T m l o V r i c w L r i n d l b s [ 6 Am f o V r i t t b u o t ( l f b i a t m a o p t [ S i h r1 t o o u o m l o V r b h ( [ i [ t h [ c ( t a [ c [ a mc t ( [ i t e G i a l a [ w i g c a a f a d t i a i a p p [ I p e a r r o t s o V p a a o c o V p i p t [ I 1 i t H H S a p t w g i m m w d i w f t g c p a r i p a a d t i d o c h d [ T d h b i u a o t w f a t d b t p m o t e f h n y b c O s w d t e t e o g t o V r m l i r a t g s i i t m n o t e o t c i d
2 M
a
m
2
Animals
a
c
m o
J w
T w (
J
w o T
r f r
( J w
f
k S a l
7
pC i d ( T J a i t w a h i a- r n ’ f t a h I s Er w p f 6 t 1 f 7 o 1 d 2i mu o p i 1 i s A g w a w a f s t 1 w r w a t 1 A e G o a a v f t s o p w k p b W J a S (e r J [ iw p T f N E N e oi ( J E a w an r g , b p 2 Blood sampling and measurement of serum e n lipids nl x f o a O d O 7 a 1 a o p i oe r f t g p a e a c g w wl s p t ae b w s f a o e s l hl T w d f ot r f i l e n a ys a – u u T t a T c8 ac l w m w e k ( e E W a T W rt i G W e C Ti J y H d l ( c e w d b m ( d tt p r he k D C T J o r -g c n m g o C g b ( ‘ c c
2
c
e c
f
I
T R t a g f ( N b s p l o r [ 6 b P f [ c a c f [ l l w u R D M c o St
o R w
a i r
m a [
N f
e
be l
e lr
eo
e de e u h
u
oe hh G e n a a c
a i
i r
a t
r b h
e
t o C w p A p
e
e
a[ 2
la k
d f
r c f Ir L r c b aE r l A s ( c a 1 b E h h ( [ c f / ( p a b C [ w u a a i b m l A
m f
V
1 9
e
i
a
223
N. A4atsuokaet al. ] Atherosclerosis126 (1996)221-226
m
VLDL-R 1 N
b d
t
a o i
o c V
r a R
r r s
a T
o m o ~
a
LPL
7
ACS
o V r . L a A m l i a t o o g n e r a o e m l ( F l t rc t R ( ,n f rt r 7 a 1 4 r w a -T m d w ha d o w fi c p ( a d i S 2 T s w r ew l e la e ( a r ~ ( p cs y M c a 2 a 1 r t o R l f V r ( L ( A (e w d b d m w u a a i c T c m a w s c t t - o
e
h a e b m e m
-e c
n
a
O
R a o a r m a w d b w a t l r e b a ( S P1 ( t 2 t 6 o 3 R w 1 t t c T m p l v o e p o m l r w m A 1 g a s a t p t T l d w I t g m r t 5 m c t t t m l o t s d T ( m i t c o s H c l o d Ow 1 m a h O d 1 t w n c o d 1 T t c ( F 2 l o d Ow 2 m a d n a a o e t 1 d a d
w g O t d r
O
❑D
❑ Day 1
#
F s a l ( ( s d
■D
p
J
o w
X f i T J o e m s i U
(
F
s
T
K
( T
a n r e t i a i p A r
(
, tn o g
eo (
f
e o g P c e f m l b N e I t r a nc p t V r m l o t c v o d 7 d 1 T l o L m o d 7 a 2 c e o d 1 2 i o w o o d 1 T ea i F I e m m t V r a e o d 7w s b d a 3 o t i t c w s a a t s l h h I t g m ( k w o m I p
f
t a
t
w
a
o
o
N. Matsuoka er al. / Atherosclerosis126 (1996)221–226
2
Day O
A
7
n
9
14 -O
k
VLDL-R 6“0kb-
3.6kb“
- 28S rRNA
‘“”
-1
D g
B
rRNA
+
~
VLDL-R
9 k . 3 ~
F 2 N b a ( ~ o t g R ( //g!lane) o l
a
v
o m m o t
l w h
f
t
h f
g w
3 N R w (
b (
y h a
a
o m o t l f w V r A ( c
l g
i (
p
m V a
r
g
r
o t 2t o 3 k a t o 9 k w o I t l v c m a o V r m l b N b a f t r a d b g a c r ( 2 I t l V r m w n d e b o a a ( 3 T L r ad A m l i t l d n c w g t
F T r r
-
t
l L
4 D T
a l v (/ a J w he
o
c ( w
V e
amn c r
r b r
(
T
sy p
( c m
R T p
i e
m f o V n i p u t b t u o T l o i n t T o o t s w i m i t p c o Va t t T l r e o g e T e i m d t t md T s o V p f i t [e a t t i r o T l f b [ O r d a d o s T l a i V r m l i a t s m a w g T l m i t r a i s d f t i t h i t r e e o V m w a o i t h ( a e a t g S e r ( t 4 i d n s T e s p t f t b s i t 5 r o t h V r g s a S b s s r e s D m f h t f a a h s o e r e [ N p s o t g h b r y W p p I a o t 5 r o h V r o n g N s i g w o e m b g t T y ai c ec a ( m e lm c l o i C b
l e p t r b o l c m o a t m o r m o 2
e
e e
f r
be
N. Matsuoka et al. / Atherosclerosis126 (1996)221-226
c t w t 5 r c i c ( n s W c d t t r b t r rb s o t r h t d m r t a o d p p r e F t e o g o V r m s o h l h n b e A p t m o u o V r m l b g i s u L i a k e i T l h a w h o t p i o L a V r i o p s W a w o i t t i f c o L w V r i u o T l A i a a r e i b t s a u o T a f f a a e s T e o g o L a A h b r e [ I o s b L a A m l i a t w f t b u b g T i c w t u t o V r m a i t c o T l f t b S e a r u o A i t l k a a t b f a d [ T d b t r a o m b d t c c s a f t d o t d o o R W e a r t V r e w e i J c ( c c l b c w i a af a d [ T u o V r m m b ac e o f a d b t e o o p p o V r m h n y r b r I t l i o s V r w n e i c r M g d n i VLDL r t n d i c e L o o A m l T d b t r i t V r m i n e i v b i e i H c l [ s h s r t m t m o H T d o T l s b w n c b t s
225
Af i h r ’ o i 3 n r t o Vo i a L r m [ G e a re a t t p i a t i d o [ I o e o o s t w 2 b s ier a ai b t o eb w u3 a l t o 9 T ce o t t b s t t e m p v i p i o t tx r F b w o o u p a6 ee i t g a n i o a n em s m s a t s m ( i n s I t g t y o i t h b d q o t 6 nb ae c i p w t o t o t t rn t t w s t s ou c i t m p m I s c i ( r ec [ A ar v s m c l L t r m c tl 9 t w ot l T t a et ob s nf o V r m O c t c le o N b v i t s b t e o t d a n pc d w c t l o p m o e I c ot V r m a i b t a e n i o g f l i ir T e G e oc V rp m i i c e t w L a A p r t t i i c o T e il r o a e A c i e x c n W g a o a o l i P T K f h h a A w e p a w t t M M S M S e I a M M K f t e e e r l s w T r w s i e o e p b r g f t H C e R R F e x e o r D R i e [ T S K Y eN T S J Y a e a m T R vx l d l rx a l d l r p w d ea ri e l s P N A S U 1 p e a 9 i y y l p g [ G M C M B D J C P D S
I
J
C
o a c
e
a p
2
N. Matsuoka et al. / Atherosclerosis126(1996)221-226
h v l d l i E e p Bp R C 1 o 2 t a a o t g t c 9 s p [ W F G M B h M L S C M G 1 1 a r o t 9h v l d l [ S a r p J H A T S e a S c at t e op s l a e o t h v l d a r f to r x i p l t E l r g JB C 1 2 i e p d 1 16 o [ J E L K W K H Y F a J o [ M H H Y T N K E P H H R o t v l d o o v el d l r m f l r b t h i r s i p h l i r h F Le 1 [ I 9 T Y K S u J e a P c –9 m JB C 1 1 K C M M v s ,r [ O K l t e b eh g eh s r ( c i c t t e po y V c f i r i o l s e a e r w t x v o m p e e f a a i i n T l ro Eur J B w e A d m f -V r 1 2 2 4 1 1 4 [ S R O J K P E o g o J P D J M K B S A [ W C a nh o t h d il r s a t a at m l M ‘ l d l ( r m i e e 1 9 [ L M I D O J C H M G 1 3 e o g a c 1a h M P L B C e a C o a ~ i t [ G p A c d a e t om e o 1m 1 nr t [ T Pp W A G h v l d l io oar mo i p pe d ps a t e p a a o t g a h Er eax p u i dhn D 1 3 y t m c 1 E 1 1 d 3 [ R G U P M R T h e o i l f P aR H H M K M e a C o ea [ B g i b b r m y s a r9 S M 1 6 [ I I G S A o t sa o t L r f E J 1 1 6 1 e o am h r s pe H Y T T V r w abi p [ J 1 3 b o t L C O L 1 DN r 9 L [ F M E O H K e a H H S 1 p wa g a r i mo T H A T S K Y a t [10] Y m hw d NE J M 1i 3 I H S J T v l d l i e y [ l c i N S r i t m o p e P eS C m o R h i b a g h i a A N A S 1 1 9 t f e A B 1 J H U R S M O G H y1 a a [11] H [ M H O Y I N te a o H S K S l a h a o c o u Obese g f e a e e a r d a 5 l m p c r t t 0d rx f t D L s a p r a l D h i t a i 1L u y [ Y T B R9 B M G9 J r E J 1 1 e R D i c ra o L Drosophila S C L S M A T c D [ r ii to t c s i Wn re e y g e av r b t S 2 3 t l d l r s P N i 1 u e e [ A S U 1 9 1 4 Y K S H K J e a S a r o r il a J B [ S A H C R L e a V e as C i 1 2 6 p l d I r f m g a e e [ W Kp K T La A S M Ln e m e c l b a m e R H l c e D sr s o M 4 r a p F I sI q S 1 25 1 3 2 [30] H D W P M~ N a [ B F G M F D e a T 3 iD o t r a a / r p r e I b b t c s e e r a m G 1 1 v l d l r J B C i e e [31] S K S9 B G P A J A 1 2 6 : s m a e i c b f u [ S J T S K L a a c , d f o a l p E J B he f c f i C H o c o a 1 1 p v l d l r B ri e e