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ELISPOT assay: Is it a suitable alternative for PPD skin test?
S140
Abstracts
Keywords: Caloric restricion, MDA, 8-isoPGF2, Protein carbonyl, 8-OHdGua doi:10.1016/j.clinbiochem.2011.08.338
Poster — [A-10-312-1] Paraoxonase phenotype distribution in exudative age related macular degeneration and its relationship with homocysteine and oxidized low density lipoprotein Elham Bahreini, Amir Ghorbanihaghjo, Alireza Javadzadeh, Nadereh Rashtchizadeh, Samira Alizadeh Biotechnology Research Center and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran E-mail addresses: [email protected] (E. Bahreini), [email protected] (A. Ghorbanihaghjo), [email protected] (A. Javadzadeh), [email protected] (N. Rashtchizadeh), [email protected] (S. Alizadeh) Introduction: Disequilibrium between body oxidants and antioxidants status has been proposed as one of the main risk factors of the disease. The aim of the present study was to investigate homocysteine (Hcy) levels within paraoxonase 1 (PON1) phenotypes in the patients with exudative Age Related Macular Degeneration (ARMD). Materials and methods: Serum PON1 activity and plasma Hcy and OX-LDL levels were analyzed in 45 exudative ARMD patients and 45 healthy controls. The PON1 phenotype was determined using double substrate method. Hcy and OX-LDL levels were determined by ELISA method. Results: The distribution of PON1 phenotypes was significantly different between the patients with exudative ARMD and control subjects (chi-square = 6.17, P = 0.01). The frequencies of PON1 phenotypes in the patients compared with controls were as AAphenotype ( low activity ) 62.2% vs. 35.6% , AB-phenotype (intermediate activity) 28.9% vs. 46.7% and BB-phenotype (high activity) 8.9% vs. 17.8%, respectively. Except in BB, patients with AA and AB phenotypes had higher Hcy levels (P < 0.05). The mean OXLDL levels, in all three phenotypes (P < 0.05), and OX-LDL/HDL ratio, in AA and AB phenotypes (P < 0.05), were significantly higher in the patients. No significant differences were found in Hcy and OX-LDL levels (P > 0.05) among three phenotypes. Conclusion: Increased plasma oxidized LDL levels as an index of oxidative stress in exudative ARMD patients may be due to higher levels of homocysteine. Moreover paraoxonase activity, even in highactivity phenotype, is not sufficient in prevention of exudative ARMD disease; because exudative ARMD is a multifactorial disease. Keywords: Exudative age-related macular degeneration, Paraoxonase, Homocysteine, Oxidized LDL doi:10.1016/j.clinbiochem.2011.08.339
Poster – [A-10-341-1] ELISPOT assay: Is it a suitable alternative for PPD skin test? Mina Hemmatia, Atefeh Seghatoleslama, Mojgan Rastia, Saeedeh Ebadata, Nader Mosavarib, Mojtaba Habibagahia, Mohammad Taheria, Ahmad-reza Sardariana, Zohreh Mostafavi-Poura a Shiraz University of Medical Sciences, Iran b Razi Vaccine, Serum Research institute, Tehran, Iran E-mail addresses: [email protected] (M. Hemmati), [email protected] (A. Seghatoleslam), [email protected] (M. Rasti), [email protected] (S. Ebadat),
[email protected] (N. Mosavari), [email protected] (M. Habibagahi), [email protected] (M. Taheri), [email protected] (A.-r. Sardarian), [email protected] (Z. Mostafavi-Pour) Introduction: One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M.tb) genome sequence has led to the identification of several antigens that can be utilized for accurate diagnosis and control of TB. The aim of this study is evaluation of produced recombinant M.tb antigens, from native strain in Iran, in TB diagnosis. Materials and methods: To investigate the antigen specific responses of the recombinant secretory antigens, ESAT-6, CFP-10 and ESAT-6/CFP-10 which were produced by PCR and cloning methods, ex vivo enzyme linked immunospot (ELISPOT) test in 30 clinically diagnosed TB patients to compare with the controls was evaluated. Results: Throughout this work, the selected M.tb antigens were produced. ELISPOT assay for detection of TB, showed sensitivity of 93, 90 and 100% and specificity of 100, 96 and 100% for recombinant ESAT-6, CFP-10 and ESAT-6/CFP-10 proteins, respectively, which is significantly higher than conventional TST. Conclusion: According to our results, these recombinant antigens can be used as an accurate means of detecting TB in Iran. Keywords: Mycobacterium tuberculosis, ELISPOT, CFP-10, ESAT-6, TB diagnosis doi:10.1016/j.clinbiochem.2011.08.340
Poster — [A-10-347-1] Association of pro oxidant–antioxidant balance with the severity of coronary artery disease Hossein Pourghadamyaria, Majid Ghayour-Mobarhanb, Mohammad Reza Parizadeha, Mohsen Mohebatic, Amir Hossein Sahebkard, Shima Tavalaeia, Roghayeh Paydara, Amir Ali Rahsepare a Departmant of Biochemistry of Mashhad University of Medical Sciences, Iran b Biochemical and Nutritional Research Centre, Departmant of Nutrition of Mashhad University of Medical Sciences, Mashhad, Iran c Department of Cardiology, Faculty of Medicine, MUMS, Mashhad, Iran d Pharmacy Faculty, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran e Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran E-mail addresses: [email protected] (H. Pourghadamyari), [email protected] (M. Ghayour-Mobarhan), [email protected] (M.R. Parizadeh), [email protected] (M. Mohebati), [email protected] (A.H. Sahebkar), [email protected] (S. Tavalaei), [email protected] (R. Paydar), [email protected] (A.A. Rahsepar) Introduction: Oxidative stress is an imbalance between the production of pro-oxidants and antioxidant defenses in favor of pro-oxidants. It is usually related to the increased formation of reactive oxygen species (ROS), and is thought to play a pivotal role in the pathogenesis and development of CVD and its complications. It is suggested that oxidative stress may be a strong and independent prognostic predictor of cardiovascular events and has been recently proposed as a significant risk factor for cardiovascular diseases. The purpose of the this study was to investigate the relationship between the oxidative stress and severity of atherosclerosis in patients with cardiovascular disease (CAD) using a pro-oxidant–antioxidant balance (PAB) assay.
Abstracts
Keywords: Caloric restricion, MDA, 8-isoPGF2, Protein carbonyl, 8-OHdGua doi:10.1016/j.clinbiochem.2011.08.338
Poster — [A-10-312-1] Paraoxonase phenotype distribution in exudative age related macular degeneration and its relationship with homocysteine and oxidized low density lipoprotein Elham Bahreini, Amir Ghorbanihaghjo, Alireza Javadzadeh, Nadereh Rashtchizadeh, Samira Alizadeh Biotechnology Research Center and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran E-mail addresses: [email protected] (E. Bahreini), [email protected] (A. Ghorbanihaghjo), [email protected] (A. Javadzadeh), [email protected] (N. Rashtchizadeh), [email protected] (S. Alizadeh) Introduction: Disequilibrium between body oxidants and antioxidants status has been proposed as one of the main risk factors of the disease. The aim of the present study was to investigate homocysteine (Hcy) levels within paraoxonase 1 (PON1) phenotypes in the patients with exudative Age Related Macular Degeneration (ARMD). Materials and methods: Serum PON1 activity and plasma Hcy and OX-LDL levels were analyzed in 45 exudative ARMD patients and 45 healthy controls. The PON1 phenotype was determined using double substrate method. Hcy and OX-LDL levels were determined by ELISA method. Results: The distribution of PON1 phenotypes was significantly different between the patients with exudative ARMD and control subjects (chi-square = 6.17, P = 0.01). The frequencies of PON1 phenotypes in the patients compared with controls were as AAphenotype ( low activity ) 62.2% vs. 35.6% , AB-phenotype (intermediate activity) 28.9% vs. 46.7% and BB-phenotype (high activity) 8.9% vs. 17.8%, respectively. Except in BB, patients with AA and AB phenotypes had higher Hcy levels (P < 0.05). The mean OXLDL levels, in all three phenotypes (P < 0.05), and OX-LDL/HDL ratio, in AA and AB phenotypes (P < 0.05), were significantly higher in the patients. No significant differences were found in Hcy and OX-LDL levels (P > 0.05) among three phenotypes. Conclusion: Increased plasma oxidized LDL levels as an index of oxidative stress in exudative ARMD patients may be due to higher levels of homocysteine. Moreover paraoxonase activity, even in highactivity phenotype, is not sufficient in prevention of exudative ARMD disease; because exudative ARMD is a multifactorial disease. Keywords: Exudative age-related macular degeneration, Paraoxonase, Homocysteine, Oxidized LDL doi:10.1016/j.clinbiochem.2011.08.339
Poster – [A-10-341-1] ELISPOT assay: Is it a suitable alternative for PPD skin test? Mina Hemmatia, Atefeh Seghatoleslama, Mojgan Rastia, Saeedeh Ebadata, Nader Mosavarib, Mojtaba Habibagahia, Mohammad Taheria, Ahmad-reza Sardariana, Zohreh Mostafavi-Poura a Shiraz University of Medical Sciences, Iran b Razi Vaccine, Serum Research institute, Tehran, Iran E-mail addresses: [email protected] (M. Hemmati), [email protected] (A. Seghatoleslam), [email protected] (M. Rasti), [email protected] (S. Ebadat),
[email protected] (N. Mosavari), [email protected] (M. Habibagahi), [email protected] (M. Taheri), [email protected] (A.-r. Sardarian), [email protected] (Z. Mostafavi-Pour) Introduction: One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M.tb) genome sequence has led to the identification of several antigens that can be utilized for accurate diagnosis and control of TB. The aim of this study is evaluation of produced recombinant M.tb antigens, from native strain in Iran, in TB diagnosis. Materials and methods: To investigate the antigen specific responses of the recombinant secretory antigens, ESAT-6, CFP-10 and ESAT-6/CFP-10 which were produced by PCR and cloning methods, ex vivo enzyme linked immunospot (ELISPOT) test in 30 clinically diagnosed TB patients to compare with the controls was evaluated. Results: Throughout this work, the selected M.tb antigens were produced. ELISPOT assay for detection of TB, showed sensitivity of 93, 90 and 100% and specificity of 100, 96 and 100% for recombinant ESAT-6, CFP-10 and ESAT-6/CFP-10 proteins, respectively, which is significantly higher than conventional TST. Conclusion: According to our results, these recombinant antigens can be used as an accurate means of detecting TB in Iran. Keywords: Mycobacterium tuberculosis, ELISPOT, CFP-10, ESAT-6, TB diagnosis doi:10.1016/j.clinbiochem.2011.08.340
Poster — [A-10-347-1] Association of pro oxidant–antioxidant balance with the severity of coronary artery disease Hossein Pourghadamyaria, Majid Ghayour-Mobarhanb, Mohammad Reza Parizadeha, Mohsen Mohebatic, Amir Hossein Sahebkard, Shima Tavalaeia, Roghayeh Paydara, Amir Ali Rahsepare a Departmant of Biochemistry of Mashhad University of Medical Sciences, Iran b Biochemical and Nutritional Research Centre, Departmant of Nutrition of Mashhad University of Medical Sciences, Mashhad, Iran c Department of Cardiology, Faculty of Medicine, MUMS, Mashhad, Iran d Pharmacy Faculty, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran e Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran E-mail addresses: [email protected] (H. Pourghadamyari), [email protected] (M. Ghayour-Mobarhan), [email protected] (M.R. Parizadeh), [email protected] (M. Mohebati), [email protected] (A.H. Sahebkar), [email protected] (S. Tavalaei), [email protected] (R. Paydar), [email protected] (A.A. Rahsepar) Introduction: Oxidative stress is an imbalance between the production of pro-oxidants and antioxidant defenses in favor of pro-oxidants. It is usually related to the increased formation of reactive oxygen species (ROS), and is thought to play a pivotal role in the pathogenesis and development of CVD and its complications. It is suggested that oxidative stress may be a strong and independent prognostic predictor of cardiovascular events and has been recently proposed as a significant risk factor for cardiovascular diseases. The purpose of the this study was to investigate the relationship between the oxidative stress and severity of atherosclerosis in patients with cardiovascular disease (CAD) using a pro-oxidant–antioxidant balance (PAB) assay.