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Engineering proteins that bind, move, make and break DNA
665
Erratum
Engineering proteins that bind, move, make and break DNA$ Cynthia H Collins, Yohei Yokobayashi, Daisuke Umeno and Frances H Arnold Current Opinion in Biotechnology 2003, 14:371–378 0958-1669/$ – see front matter ß 2003 Elsevier Ltd. All rights reserved.
methods, whereas a third described the substitution of an entire a-helix to alter the DNA-binding specificity of the 434 repressor protein [3]. The authors regret this significant oversight.
DOI 10.1016/j.copbio.2003.10.002
References In this article, published in the August 2003 issue of Current Opinion in Biotechnology, the author’s discussion of engineering a-helical DNA-binding domains failed to note earlier reports of engineering protein–DNA interactions [1–3]. In particular, two studies reported altering the DNA-binding specificities of the Mnt repressor [1] and catabolite activator protein [2], using genetic selection
$
1.
Youderian P, Vershon A, Bouvier S, Sauer RT, Susskind MM: Changing the DNA-binding specificity of a repressor. Cell 1983, 35:777-783.
2.
Ebright RH, Cossart P, Gicquelsanzey B, Beckwith J: Mutations that alter the DNA-sequence specificity of the catabolite gene activator protein of E. coli. Nature 1984, 311:232-235.
3.
Wharton RP, Brown EL, Ptashne M: Substituting an a-helix switches the sequence-specific DNA interactions of a repressor. Cell 1984, 38:361-369.
PII of original article: S0958-1669(03)00091-0
www.current-opinion.com
Current Opinion in Biotechnology 2003, 14:665
Erratum
Engineering proteins that bind, move, make and break DNA$ Cynthia H Collins, Yohei Yokobayashi, Daisuke Umeno and Frances H Arnold Current Opinion in Biotechnology 2003, 14:371–378 0958-1669/$ – see front matter ß 2003 Elsevier Ltd. All rights reserved.
methods, whereas a third described the substitution of an entire a-helix to alter the DNA-binding specificity of the 434 repressor protein [3]. The authors regret this significant oversight.
DOI 10.1016/j.copbio.2003.10.002
References In this article, published in the August 2003 issue of Current Opinion in Biotechnology, the author’s discussion of engineering a-helical DNA-binding domains failed to note earlier reports of engineering protein–DNA interactions [1–3]. In particular, two studies reported altering the DNA-binding specificities of the Mnt repressor [1] and catabolite activator protein [2], using genetic selection
$
1.
Youderian P, Vershon A, Bouvier S, Sauer RT, Susskind MM: Changing the DNA-binding specificity of a repressor. Cell 1983, 35:777-783.
2.
Ebright RH, Cossart P, Gicquelsanzey B, Beckwith J: Mutations that alter the DNA-sequence specificity of the catabolite gene activator protein of E. coli. Nature 1984, 311:232-235.
3.
Wharton RP, Brown EL, Ptashne M: Substituting an a-helix switches the sequence-specific DNA interactions of a repressor. Cell 1984, 38:361-369.
PII of original article: S0958-1669(03)00091-0
www.current-opinion.com
Current Opinion in Biotechnology 2003, 14:665