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Erratum to “Amplification, cloning and sequencing of the σC-encoded gene of avian reovirus” [J. Virol. Methods 63 (1997) 206]
ELSEVIER
Journal of Virological Methods Journal of Virological Methods 65 (1997) 318
Erratum
Erratum to "Amplification, cloning and sequencing of the ~rC-encoded gene of avian reovirus" [J. Virol. Methods 63 (1997) 206] 1 Hung-Jen Liu, J.J.
Giambrone *
Department of Poultry Science, Alabama Agriculture Experiment Station, Poultry Annex, Woodfield Drive, Auburn University, Auburn, AL 36849-5416, USA Accepted 11 September 1996
T h e p u b l i s h e r r e g r e t s t h a t t h e l e g e n d f o r Fig. 2 w a s i n c o r r e c t l y p r i n t e d in t h e a b o v e p a p e r . T h e c o r r e c t c a p t i o n is r e p r o d u c e d b e l o w : Fig. 2. Characterization of PCR amplification products, cloning, and identification of the ~rC-encoding cDNA of ARVs. A - D . PCR amplification products of ARV ~rC gene. A. PCR products using primers SIC and S1D. B. PCR products using primers S1E and S1F. C. PCR products using primers SIG and SIH. D. PCR products using primers SII and S1H. Sizes in bp of each product is indicated on the right of each panel. DNA shown in lanes 1, 21, 3 and 23 was amplified from ARV Sl133 cDNA. DNA shown in lanes 2, 22, 4 and 24 was amplified from ARV 1733 cDNA. E - F . EcoRI-cleaved plasmid DNA of PCR products cloned into the pUC18 vector. E. Plasmids containing PCR products using primers SIC and S1D. F. Plasmids containing PCR products using primers S1E and S1F. G. Plasmids containing PCR products using primers S1G and S1H. H. Plasmids containing PCR products using primers SII and S1H. Arrows indicate cDNA inserts. Lanes are same as in panels A - D . I. Restriction enzyme analysis of PCR products. Lane 1, PCR product of ARV S1133 cDNA using primers SIC and S1D, cleaved with TaqI; Lane 2, PCR product of ARV 1733 cDNA using primers SIC and S1D, cleaved with TaqI; Lanes 3, PCR product of ARV S1133 cDNA using primers S1G and S1H, cleaved with PstI; Lanes 4, PCR product of ARV 1733 cDNA using primers S1G and S1H, cleaved with PstI. Sizes of fragments are indicated in bp. J. Southern blot of PCR products. Lanes correspond to lanes in panels A and C. Molecular weight markers (M) include DNA marker 50-1000 bp and EcoRI digested SPP1-DNA.
* Corresponding author. 1 PII of original article: S0166-0934(96)02129-5 0166-0934/97/$17.00 © 1997 Elsevier Science B.V. All rights reserved. PII SO 166-09 34(97)02209-X
Journal of Virological Methods Journal of Virological Methods 65 (1997) 318
Erratum
Erratum to "Amplification, cloning and sequencing of the ~rC-encoded gene of avian reovirus" [J. Virol. Methods 63 (1997) 206] 1 Hung-Jen Liu, J.J.
Giambrone *
Department of Poultry Science, Alabama Agriculture Experiment Station, Poultry Annex, Woodfield Drive, Auburn University, Auburn, AL 36849-5416, USA Accepted 11 September 1996
T h e p u b l i s h e r r e g r e t s t h a t t h e l e g e n d f o r Fig. 2 w a s i n c o r r e c t l y p r i n t e d in t h e a b o v e p a p e r . T h e c o r r e c t c a p t i o n is r e p r o d u c e d b e l o w : Fig. 2. Characterization of PCR amplification products, cloning, and identification of the ~rC-encoding cDNA of ARVs. A - D . PCR amplification products of ARV ~rC gene. A. PCR products using primers SIC and S1D. B. PCR products using primers S1E and S1F. C. PCR products using primers SIG and SIH. D. PCR products using primers SII and S1H. Sizes in bp of each product is indicated on the right of each panel. DNA shown in lanes 1, 21, 3 and 23 was amplified from ARV Sl133 cDNA. DNA shown in lanes 2, 22, 4 and 24 was amplified from ARV 1733 cDNA. E - F . EcoRI-cleaved plasmid DNA of PCR products cloned into the pUC18 vector. E. Plasmids containing PCR products using primers SIC and S1D. F. Plasmids containing PCR products using primers S1E and S1F. G. Plasmids containing PCR products using primers S1G and S1H. H. Plasmids containing PCR products using primers SII and S1H. Arrows indicate cDNA inserts. Lanes are same as in panels A - D . I. Restriction enzyme analysis of PCR products. Lane 1, PCR product of ARV S1133 cDNA using primers SIC and S1D, cleaved with TaqI; Lane 2, PCR product of ARV 1733 cDNA using primers SIC and S1D, cleaved with TaqI; Lanes 3, PCR product of ARV S1133 cDNA using primers S1G and S1H, cleaved with PstI; Lanes 4, PCR product of ARV 1733 cDNA using primers S1G and S1H, cleaved with PstI. Sizes of fragments are indicated in bp. J. Southern blot of PCR products. Lanes correspond to lanes in panels A and C. Molecular weight markers (M) include DNA marker 50-1000 bp and EcoRI digested SPP1-DNA.
* Corresponding author. 1 PII of original article: S0166-0934(96)02129-5 0166-0934/97/$17.00 © 1997 Elsevier Science B.V. All rights reserved. PII SO 166-09 34(97)02209-X