- Email: [email protected]
Erythritol production from raw materials by Yarrowia lipolytica
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Abstracts / Journal of Biotechnology 231S (2016) S4–S109
fruit feeding water. The useful markers to differentiate fruits origin were ␦18 O, ␦2 H, ␦13 C, Li, Cr and Cu. The observed differences between stable isotopes content of fruit juices are explained by the altitude and precipitation values associated with each geographic origin. Therefore, isotopic and elemental fingerprinting may be an adequate approach to discriminate natural fruit juices. http://dx.doi.org/10.1016/j.jbiotec.2016.05.280 Erythritol production from raw materials by Yarrowia lipolytica Anita Rywinska ∗ , Ludwika Tomaszewska Hetman, Waldemar Rymowicz Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland
The study was conducted on 15 samples of meat products (smoked pork sausages, smoked pork, smoked thick pork sausages, smoked bacon and thin pork smoked sausages), taken under the self-control type program, analyzed and approved for Listeria monocytogenes and Salmonella germs identification. During laboratory tests the presence of Listeria monocytogenes was not found in any sample and at the sample represented by thick smoked pork sausages was Salmonella spp. Identified. As conclusions, in any sample analyzed we have not found the presence of Listeria monocytogenes. Given the presence of Salmonella germs in an analyzed sample the withdrawal of consumer incriminated lot was ordered, the implementation of corrective actions that consisted of analyzing and improving the HACCP procedures, retraining staff, reconsideration traceability, cleaning operations and reanalyzing the samples. The positive obtained result requires corrective measures for the entire technological flow. http://dx.doi.org/10.1016/j.jbiotec.2016.05.282
E-mail address: [email protected] (A. Rywinska). Erythritol is the only polyol which is manufactured on a commercial scale from glucose or sucrose exclusively by a microbial fermentation. In order to reduce the manufacturing costs a novel strategy for erythritol production was developed on the basis of raw glycerol derived from biodiesel production and corn steep liquor (CSL), a major by-product of the corn wet milling industry. The Yarowia lipolytica A-6 strain ability of glycerol to erythritol conversion was investigated in the media with different concentrations of glycerol, mineral components, salt and CSL, which was used as source of nutritional ingredients. During the cultivations concentration of erythritol and by-products, activities of enzymes involved in the erythritol biosynthesis as well as osmotic pressure were monitored. The obtained results indicated that low-cost substrates such as raw glycerol and CSL might be used successfully for erythritol production by yeast without supplementation with expensive ingredients, which enable to reduce the production costs. Comparison of the erythritol production parameters and the activities of transketolase and erythrose reductase in a low-cost and in a complex media have shown that the applied kind of glycerol was more important than the presence of minerals. In the low-cost medium in bioreactor batch cultures yeast were able to produce 108 g/l of erythritol from 220 g/l of glycerol within 104 h of cultivation. http://dx.doi.org/10.1016/j.jbiotec.2016.05.281 Study regarding the microbiological quality of smoked meat products obtained in a meat processing type unit Nistor Elena 1,∗ , Enache Valter 2 , Savu Ovidiu 1 , Savu Constantin 1 1 University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 D.S.V.S.A. Brasov, Romania
E-mail address: [email protected] (N. Elena). The conducted study focused on highlighting the importance of microbiological quality analysis of smoked meat products obtained in a specialized butcher type establishment. Concerning the importance of these products for human nutrition and because the food placed on the market must be safe for consumption, microbiological monitoring is an essential step because of the multitude of risk factors that may arise and jeopardize consumers’ health.
Transfusion and post-transfusion reactions in dogs undergoing hemodialysis Catalin Constantin Ivascu ∗ , Alexandru Bogdan Vitalaru, Stefanita Bogdan Balascau, Alexandru Nicolae Bociu, Alexandru Sonea Department of Clinical Science, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (C.C. Ivascu). The objective of the study is to determine if dogs undergo hemodialysis present more transfusion risks depending on the blood product that was used. Six dogs suffering from acute anemia, three dogs were managed by administrating 5 ml/kg of hole blood, and another three dogs that received 5 ml/kg erythro-concentrate (packed red cells), that was re-suspended in 100 ml physiological saline, after dialysis, were monitored during and after transfusion for 48 h. All six dogs at the beginning were suffering of acute renal failure and anemia provoked by a large infestation with Babesia Canis spp. The anemic syndrome was worsened by repeated dialysis in a 48-h interval, and appeared after the 5th session. During the 48 h post-transfusions there were monitored the attitude, rectal temperature, puls rate and quality, respiratory rate and character, mucous membrane color, capillary refill time, blood pressure. The tree dogs that received packed red cell experienced during transfusion various reactions, elevated blood pressure, vomiting, moderate to severe dyspnea, increased respiratory rate, and one dog experienced blood thickening, and after 24 h a hemorrhagic stroke. The three dogs that received hole blood only one experienced mild reactions during transfusion with vomiting, mild dyspnea, and were stopped by reducing the rate of transfusion. The evidence provided by these cases determined that the usage of blood product on dialysis patients should be monitored more than 24 h after transfusion and pre-medicated with steroids to decrease the immunological transfusion reactions. http://dx.doi.org/10.1016/j.jbiotec.2016.05.283
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
fruit feeding water. The useful markers to differentiate fruits origin were ␦18 O, ␦2 H, ␦13 C, Li, Cr and Cu. The observed differences between stable isotopes content of fruit juices are explained by the altitude and precipitation values associated with each geographic origin. Therefore, isotopic and elemental fingerprinting may be an adequate approach to discriminate natural fruit juices. http://dx.doi.org/10.1016/j.jbiotec.2016.05.280 Erythritol production from raw materials by Yarrowia lipolytica Anita Rywinska ∗ , Ludwika Tomaszewska Hetman, Waldemar Rymowicz Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland
The study was conducted on 15 samples of meat products (smoked pork sausages, smoked pork, smoked thick pork sausages, smoked bacon and thin pork smoked sausages), taken under the self-control type program, analyzed and approved for Listeria monocytogenes and Salmonella germs identification. During laboratory tests the presence of Listeria monocytogenes was not found in any sample and at the sample represented by thick smoked pork sausages was Salmonella spp. Identified. As conclusions, in any sample analyzed we have not found the presence of Listeria monocytogenes. Given the presence of Salmonella germs in an analyzed sample the withdrawal of consumer incriminated lot was ordered, the implementation of corrective actions that consisted of analyzing and improving the HACCP procedures, retraining staff, reconsideration traceability, cleaning operations and reanalyzing the samples. The positive obtained result requires corrective measures for the entire technological flow. http://dx.doi.org/10.1016/j.jbiotec.2016.05.282
E-mail address: [email protected] (A. Rywinska). Erythritol is the only polyol which is manufactured on a commercial scale from glucose or sucrose exclusively by a microbial fermentation. In order to reduce the manufacturing costs a novel strategy for erythritol production was developed on the basis of raw glycerol derived from biodiesel production and corn steep liquor (CSL), a major by-product of the corn wet milling industry. The Yarowia lipolytica A-6 strain ability of glycerol to erythritol conversion was investigated in the media with different concentrations of glycerol, mineral components, salt and CSL, which was used as source of nutritional ingredients. During the cultivations concentration of erythritol and by-products, activities of enzymes involved in the erythritol biosynthesis as well as osmotic pressure were monitored. The obtained results indicated that low-cost substrates such as raw glycerol and CSL might be used successfully for erythritol production by yeast without supplementation with expensive ingredients, which enable to reduce the production costs. Comparison of the erythritol production parameters and the activities of transketolase and erythrose reductase in a low-cost and in a complex media have shown that the applied kind of glycerol was more important than the presence of minerals. In the low-cost medium in bioreactor batch cultures yeast were able to produce 108 g/l of erythritol from 220 g/l of glycerol within 104 h of cultivation. http://dx.doi.org/10.1016/j.jbiotec.2016.05.281 Study regarding the microbiological quality of smoked meat products obtained in a meat processing type unit Nistor Elena 1,∗ , Enache Valter 2 , Savu Ovidiu 1 , Savu Constantin 1 1 University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania 2 D.S.V.S.A. Brasov, Romania
E-mail address: [email protected] (N. Elena). The conducted study focused on highlighting the importance of microbiological quality analysis of smoked meat products obtained in a specialized butcher type establishment. Concerning the importance of these products for human nutrition and because the food placed on the market must be safe for consumption, microbiological monitoring is an essential step because of the multitude of risk factors that may arise and jeopardize consumers’ health.
Transfusion and post-transfusion reactions in dogs undergoing hemodialysis Catalin Constantin Ivascu ∗ , Alexandru Bogdan Vitalaru, Stefanita Bogdan Balascau, Alexandru Nicolae Bociu, Alexandru Sonea Department of Clinical Science, Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (C.C. Ivascu). The objective of the study is to determine if dogs undergo hemodialysis present more transfusion risks depending on the blood product that was used. Six dogs suffering from acute anemia, three dogs were managed by administrating 5 ml/kg of hole blood, and another three dogs that received 5 ml/kg erythro-concentrate (packed red cells), that was re-suspended in 100 ml physiological saline, after dialysis, were monitored during and after transfusion for 48 h. All six dogs at the beginning were suffering of acute renal failure and anemia provoked by a large infestation with Babesia Canis spp. The anemic syndrome was worsened by repeated dialysis in a 48-h interval, and appeared after the 5th session. During the 48 h post-transfusions there were monitored the attitude, rectal temperature, puls rate and quality, respiratory rate and character, mucous membrane color, capillary refill time, blood pressure. The tree dogs that received packed red cell experienced during transfusion various reactions, elevated blood pressure, vomiting, moderate to severe dyspnea, increased respiratory rate, and one dog experienced blood thickening, and after 24 h a hemorrhagic stroke. The three dogs that received hole blood only one experienced mild reactions during transfusion with vomiting, mild dyspnea, and were stopped by reducing the rate of transfusion. The evidence provided by these cases determined that the usage of blood product on dialysis patients should be monitored more than 24 h after transfusion and pre-medicated with steroids to decrease the immunological transfusion reactions. http://dx.doi.org/10.1016/j.jbiotec.2016.05.283